79 results about "Human keratinocyte" patented technology

Enrichment for human Keratinocyte Stem Cells KSCs to a high degree of purity can be successfully achieved on the basis of a cell surface component whose expression is proliferation-related in conjunction with an integrin such as the alpha6beta4 integrin. Transferrin receptor may be used as the cell surface component that is proliferation related. Enrichment of Transit amplifying cells can also be achieved by use of a variation of this method. The enrichment follows on from harvesting of cells from an epithelium, preferably rich in stem cells.

A skin care composition comprising at least one keratinocyte CLOCK or PER1 gene activator and at least one DNA repair enzyme; a method for inhibiting damage to human keratinocytes due to environmental aggressors by applying a composition comprising at least one keratinocyte CLOCK or PER1 gene activator and at least one DNA repair enzyme; and a method for repairing DNA damage in human keratinocytes.

The present invention relates to an ex vivo method for obtaining a population of human keratinocytes derived from human pluripotent stem cells comprising a step of co-culturing human pluripotent stem cells with cells that support ectodermal differentiation in presence of an agent that stimulates epidermal induction and a agent that stimulates terminal differentiation of keratinocytes. A further object of the invention relates to a method for preparing a human skin substitute comprising a step of providing an organotypic culture of the substantially pure homogenous population of human keratinocytes derived from human pluripotent stem cells obtained according to the method of the invention.

A chimeric skin comprising immortalized human keratinocyte cells cocultured with donor keratinocytes is disclosed.

The invention relates to a method for preparing relaxing and face-cleansing cosmetics and application. The cosmetics comprises the following components in percentage by mass: 0.00001 to 0.001 percent of recombinant human keratinocyte growth factors, 0.00001 to 0.001 percent of recombinant human interleukin-1 receptor antagonist, 0.1 to 0.5 percent of low-molecular sodium heparin, 5 to 25 percent of stabilizer, 0.02 to 0.3 percent of hyaluronic acid, 0.5 to 8 percent of vitamin C sodium phosphate, 0.5 to 5 percent of methyl propanediol, 0.3 to 0.7 percent of 1,2 hexanediol, 0.5 to 3.5 percent of lactobacillus / mung bean fermentation liquor, 0.5 to 5 percent of dissolved protease and the balance of water. The relaxing and face-cleansing cosmetics are applied to sensitive skin, can be stored for a long time, and is suitable for long-term use.

The present invention relates to a TGF-β1 binding protein called r150. This protein has a GPI-anchor contained in r150 itself and not on a tightly associated protein and that it binds TGF-β1 with an affinity comparable to those of the signaling receptors. Furthermore, the released (soluble) form of this protein binds TGF-β1 independent of the types I and II receptors. Also, the soluble form inhibits the binding of TGF-β to its receptor. In addition, evidence that r150 is released from the cell surface by an endogenous phospholipase C is provided. Also, the creation of a mutant human keratinocyte cell line with a defect in GPI synthesis which displays reduced expression of r150 is described. Our results using these mutant keratinocytes suggest that the membrane anchored form of r150 is a negative modulator of TGF-beta responses. These findings, taken together with the observation that r150 forms a heteromeric complex with the signaling receptors, suggest that this accessory receptor in either its membrane anchored or soluble form may antagonize TGF-β responses in human keratinocytes. Experiments with mutants confirmed that TGFβ1 activity can be modulated when the expression of the accessory receptor r150 is silenced. The complete nucleic acid and deduced amino acid sequences are now provided. The r150 cloned nucleic acid was used to study overexpression of r150. When r150 gene is overexpressed, TGFβ responses are increased. r150 and its derivatives or precursors (fragments, variants and nucleic acids encoding the same) will find a broad clinical utility, knowing that TGFβ1 is an important cytokine.

The invention discloses a water-soluble microemulsion. The water-soluble microemulsion is characterized in that the microemulsion comprises cannabidiol, a surfactant, a cosurfactant and water, and comprises the following components in percentage by weight of 0.5-5wt% of industrial hemp full-spectrum oil, 2 to 40 wt% of PPG-13-decyl tetradecyl alcohol polyether-24; 2-40 wt% of PEG / PPG / polybutylene glycol-8 / 5 / 3 glycerol, and the balance of water. The microemulsion disclosed by the invention is clear and transparent in color, is uniformly distributed into liquid drops with the particle size of about 10-30nm, is good in thermodynamic stability, and is not layered after being stored for a long time. Meanwhile, the microemulsion can effectively inhibit hyaluronidase activity, inhibit secretion of human keratinocyte IL-8 and promote generation of IL-10, remarkably inhibit growth of staphylococcus aureus and propionibacterium acnes and remove free radicals, and does not generate obvious blood coagulation or hemolysis reaction, so that inflammatory response of skin is relieved, and anti-inflammatory and soothing effects are achieved.

A human keratinocyte cell line immortalized by at least one functional tumor gene of DNA viral origin characterized in that it is: (1) non-tumorigenic, (2) conserves the capacity for differentiation and for the expression of proteins and of enzymes expressed by normal differentiated keratinocytes even after an elevated number of passages in culture; and (3) forms a stratified and polarized epithelium having a stratum corneum ortho-keratinocyte, if cultivated in an organo-typical culture in a medium without serum and without a layer of nourishing cells. An improved process to immortalize human skin cells to obtain immortalized keratinocytes. Also, the use of keratinocytes for immunological, pharmacological, photo- and chemical-toxicological analyzes of skin reaction and for expression of heterologous genes and for the construction of artificial skin.