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Preparation method of umbilical cord mesenchymal stem cell liquid for beautifying and maintenance

A technology of umbilical cord stem cells and mesenchymal stem cells, which is applied in the field of preparation of umbilical cord mesenchymal stem cell liquid, can solve the problems of not being able to effectively promote the metabolism of epidermal cells, having no biologically active substances, and many harmful substances remaining, so as to promote skin wound healing , prevent stains, and easy to operate

Inactive Publication Date: 2018-11-06
丁彬彬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the existing beauty care products are made by chemical methods, and there are many harmful substances in the products, and long-term use will have adverse effects on the skin
Moreover, most of the existing products do not have biologically active substances, which cannot effectively promote the metabolism of epidermal cells and improve the luster of the skin.
Moreover, some substances contained in chemical skin care products will cause allergic reactions to the human body, so they are not suitable for all people.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The invention provides a method for preparing an umbilical cord mesenchymal stem cell liquid for cosmetic maintenance, comprising the following steps:

[0025] Step 1: Obtain umbilical cord stem cells, obtain umbilical cord dermal tissue and umbilical cord blood, wash 2 to 5 times with normal saline at room temperature, cut the umbilical cord dermal tissue into small pieces, add phosphate buffered saline to the small pieces and mix, Centrifuge to remove the lower liquid, add umbilical cord blood, wash with phosphate buffered saline solution and centrifuge again, the centrifugation speed is 800rpm, centrifugation time is 5min, remove the supernatant, add collagenase, the collagenase in this step is 0.1% type Ⅰ collagenase , placed in a constant temperature shaking box at 37°C for 60 minutes, centrifuged again to remove the supernatant, then added DMEM culture solution containing fetal bovine serum, filtered through a cell sieve, centrifuged to remove the supernatant, and ...

Embodiment 2

[0030] The invention provides a method for preparing an umbilical cord mesenchymal stem cell liquid for cosmetic maintenance, comprising the following steps:

[0031] Step 1: Obtain umbilical cord stem cells, obtain umbilical cord dermal tissue, wash 4 times with normal saline at room temperature, cut the umbilical cord dermal tissue into small pieces, add phosphate buffered saline solution to the small pieces, centrifuge at room temperature to remove the lower liquid, add Umbilical cord blood was washed with phosphate buffered saline solution and then centrifuged again at a centrifugation speed of 1000rpm for 3 minutes. The supernatant was removed and collagenase was added. In this step, the collagenase was 0.1% type Ⅰ collagenase and placed at a constant temperature of 37°C. 30min in the shaking box, centrifuged again to remove the supernatant, then added the DMEM culture solution containing fetal bovine serum, filtered through a cell sieve and centrifuged to remove the super...

Embodiment 3

[0035] The invention provides a method for preparing an umbilical cord mesenchymal stem cell liquid for cosmetic maintenance, comprising the following steps:

[0036]Step 1: Obtain umbilical cord stem cells, obtain umbilical cord dermal tissue, wash 2 to 5 times with normal saline at room temperature, cut the umbilical cord dermal tissue into small pieces, add phosphate buffered saline solution to the small pieces, and centrifuge at room temperature to remove the lower layer Liquid, add umbilical cord blood, rinse with phosphate buffered saline and then centrifuge again, the centrifugation speed is 1500rpm, centrifugation time is 3min, remove the supernatant, add collagenase, the collagenase in this step is 0.1% type Ⅰ collagenase, place in Place in a constant temperature shaking box at 37°C for 20 minutes, centrifuge again to remove the supernatant, then add the DMEM culture solution containing fetal bovine serum, filter through a cell sieve, remove the supernatant by centrifu...

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PUM

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Abstract

The invention relates to a preparation method of umbilical cord mesenchymal stem cell liquid for beautifying and maintenance, and belongs to the technical field of medical beautifying. The method comprises the following steps of 1, obtaining umbilical cord stem cells; 2, conducting subculture; 3, preparing the umbilical cord stem cell liquid. According to the technical scheme, umbilical cord stemcells can secrete various cell growth factors to provide a good microenvironment for skin epidermal cells, wherein the cell growth factors include the fibroblastoma growth factor, the fibroblast growth factor, the hepatocyte growth factor, the insulin growth factor, angiogenin and the like; the in-vitro proliferation and migration of human keratinocytes can be activated, skin regeneration and skinwound healing are promoted when the factors enter the skin, the maturity of the skin epidermal cells can be promoted, the skin elasticity is improved, the skin is moisturized, wrinkles are eliminated, pigmentation is prevented, and the liquid has a good beautifying effect.

Description

technical field [0001] The invention relates to a preparation method of umbilical cord mesenchymal stem cell fluid for beauty maintenance, belonging to the technical field of medical cosmetology. Background technique [0002] In the modern society where the life expectancy of human beings continues to prolong and the population is aging day by day, aging and longevity issues have attracted much attention from all sides, and slowing down the aging of the skin has gradually become the goal pursued by people. The aging of the human body and the appearance of wrinkles are essentially the aging and reduction of cells. The senescence and reduction of cells is caused by the aging of stem cells. [0003] Umbilical cord mesenchymal stem cells refer to mesenchymal-like cells isolated from umbilical cord dermis and cord blood by researchers. These cells have the ability to differentiate into fat, cartilage, osteoblast and other tissue cells. They are mesenchymal stem cell family It h...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K8/99A61Q19/02A61Q19/08A61Q19/00
CPCA61K8/99A61Q19/00A61Q19/02A61Q19/08C12N5/0665C12N2501/10
Inventor 丁彬彬
Owner 丁彬彬
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