134 results about "Protein free" patented technology

A peritoneal-based (“bloodless”) artificial kidney processes peritoneal fluid without need for additional fluids (“waterless”). Fluid is separated into a protein-rich stream and a protein-free stream. The protein-rich stream is regenerated using a sorbent assembly, and its protein composition can be modified by removal of selected protein(s) (“dialysate-pheresis”). It is then reconstituted with additives and returned into the peritoneal cavity, thereby reducing protein-loss and providing oncotic-pressure for ultrafiltration. The protein-free stream is used to produce free water, and an alkaline or acid fluid for optimization of the composition of the regenerated stream. The unused protein-free stream can be used to “reverse flush” the separator to maintain its patency and the excess discarded for fluid-balance regulation. Compared to prior art, immobilization of urease allows more protein rich fluid to be regenerated and re-circulated into the peritoneal cavity for toxin removal and allows practicable development of portable and wearable artificial kidneys.

Recombinant Factor VIII can be produced in relatively large quantities on a continuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the cells in a protein free medium supplemented with polyol copolymers, preferably in the presence of trace metals such as copper. In very preferred embodiments, the medium includes a polyglycol known as Pluronic F-68, copper sulfate, ferrous sulfate/EDTA complex, and salts of trace metals such as manganese, molybdenum, silicon, lithium and chromium. With an alternative medium which included trace copper ions alone (without polyol copolymers) we were also able to enhance the productivity of Factor VIII in recombinant cells such as BHK cells that are genetically engineered to express Factor VIII.

Improvements are made to a novel media that replace the requirement for all protein growth factors by the addition to the medium of physiological concentrations of retinyl acetate. The media are serum-free, companion cell or feeder layer-free and organotypic, matrix free solutions for the cultivation of clonally competent basal keratinocytes. The media and methods are useful in the production of epidermal epithelial tissue that is suitable for skin grafting.

Protein-free creamer compositions and stabilizing systems contained therein. The creamer composition includes an emulsifying component of at least two low molecular weight emulsifiers in relative amounts sufficient to provide a stabilized emulsion, a cellulose component including a blend of microcrystalline cellulose and carboxymethylcellulose in an amount sufficient to maintain homogeneity of the composition; and a carageenan gum component present in an amount sufficient to maintain homogeneity of the composition. The creamer composition can be in the form of a shelf stable aseptic liquid creamer that is stable for at least about 9 months, an extended-shelf life (ESL) liquid creamer that is stable for at least about four months at refrigeration, or a powder that is stable for at least 24 months at ambient conditions. The creamer composition provides sufficient whitening capacity and a pleasant mouth feel without discernable feathering and without discernable fat separation when added to liquid beverages.

A peritoneal-based artificial kidney processes peritoneal fluid without need for additional fluids. Spent dialysate is separated into a protein-rich stream and a protein-free stream. The protein-rich stream is regenerated using a sorbent assembly, and its protein composition can be modified by removal of selected protein(s). Alternatively, the spent dialysate is first processed in a sorbent assembly and then separated into the protein-rich and protein-free streams. Immobilization of urease allows more protein rich fluid to be regenerated and re-circulated into the peritoneal cavity for toxin removal and allows practicable development of portable and wearable artificial kidneys.

In one embodiment, the present application discloses a cell culture medium for culturing cell lines suitable for producing a therapeutic protein, comprising an amino acid selected from a group consisting of L-arginine, L-asparagine, L-proline, L leucine and L hydroxyproline and a mixture thereof; a vitamin selected from a group consisting of ascorbic acid Mg²⁺ salt, biotin, pyridoxine HCL, folic acid, riboflavin and D-calcium pantothenate, and a mixture thereof; an element selected from a group consisting of ammonium meta vanadate, sodium meta vanadate, germanium dioxide, barium acetate, aluminum chloride, rubidium chloride, cadmium chloride, ammonium molybedate, stannous chloride, cobalt chloride, chromium sulfate, silver nitrate, sodium metasilicate, zinc sulfate, manganese sulfate H₂O, manganous chloride, ferric nitrate 9H₂O, ferrous sulfate 7H₂O, ferric ammonium citrate, magnesium chloride anhydrous, and magnesium sulfate anhydrous, and a mixture thereof; a nucleoside selected from a group consisting of uridine and cystidine; a sugar selected from a group consisting of galactose, mannose and N-Acetyl-D-Mannosamine; and a triple buffering system comprising sodium carbonate, sodium bicarbonate and HEPES; wherein the cell culture medium is animal component-free, plant component-free, serum-free, growth factors-free, recombinant protein-free, lipid-free, steroid-free, and free of plant or animal hydrolysates and/or extracts.

PCT No. PCT/CA93/00501 Sec. 371 Date Sep. 12, 1995 Sec. 102(e) Date Sep. 12, 1995 PCT Filed Nov. 23, 1993 PCT Pub. No. WO94/12641 PCT Pub. Date Jun. 9, 1994Purified and isolated nucleic acid from specific strains of Haemophilus influenzae is provided which encodes at least a portion of the D15 outer membrane protein of Haemophilus. The nucleic acid is used to produce peptides, polypeptides and proteins free of contaminant associated with Haemophilus for purposes of diagnosis and medical treatment. Furthermore, the nucleic acid may be used in the diagnosis of Haemophilus infection. Antisera obtained following immunization with the nucleic acid D15 outer membrane protein or peptides also may be used for the purpose of diagnosis and medical treatment.

The invention relates to the technical field of biology, specifically discloses a serum-free protein-free cell culture medium, and aims to provide a serum-free protein-free cell culture medium capable of supporting the growth of high density cells. The culture medium comprises amino acids, vitamins, inorganic salts, trace elements, carbohydrates, other chemical compounds, polyamine (or derivatives of polyamine), and hydrolysate. The manufacturing cost is low. The stability is high, the live cell density and cell survival rate are extremely high, and the culture medium is suitable for culture cells for recombinant protein and vaccine production.

The invention discloses an antibiotic-free homologous protein-free creep feed for weaned piglets, comprising corn, puffed corn, flour, puffed soybean, Hamlet protein, yeast nucleotide, super steamed fish meal, cheese plus cheese, sucrose, glucose, whey powder, milk powder, whey protein concentrate, calcium monohydrogen phosphate, calcium formate, edible salt, lysine, methionine, tryptophan, threonine, a compound acidifier, coated zinc oxide, coated plant essential oil, coated sodium butyrate, alkaloids, a vitamin complex, trace element premix and a compound enzyme preparation. The Hamlet protein, yeast nucleotide and whey protein concentrate are used herein to replace plasma protein powder and intestine membrane protein; attracting property, palatability and high digestive utilization areguaranteed for the antibiotic-free homologous protein-free creep feed for weaned piglets; piglets can gain smooth transition from breast milk to feed; the comprehensive utilization of the compound acidifier, coated sodium butyrate, coated zinc oxide, coated plant essential oil and alkaloids helps improve immunity in piglets and control diarrhea in the piglets.

The invention relates to a method for extracting desoxyribonucleic acid from formalin fixed and paraffin embedded tissues, and belongs to the technical field of nucleic acid application in biology. The method comprises the following steps of: preparing special lysis solution, adding the lysis solution into paraffin section tissues, boiling at high temperature for 30 minutes, and centrifuging to take supernate; adding absolute ethanol for uniform mixing, and adding the mixed solution into a silicon membrane absorption column for centrifuging; rinsing by using protein-free liquid and salt-free rinsing liquid; and eluting by using elution buffer. In the method, a toxic reagent of dimethylbenzene is not used for dewaxing, and harm to bodies of experimenters is avoided; and precious protease Kis not needed to perform long-time incubation enzymolysis, the operation is simple and quick, the extracted desoxyribonucleic acid in genome is high in quality and stability, and the cost and time can be saved to the greatest degree. The extracted product is subjected to polymerase chain reaction (PCR) detection, long segments with about 750pb can be obtained through amplification, and the work in the aspects such as scientific research, biomedicine and the like is greatly facilitated.

The invention relates to a serum-free protein-free high-efficiency feed culture medium as well as a preparation method and application thereof. The feed culture medium comprises amino acids, inorganic salts, trace elements, vitamins, carbohydrates and other organic matters. Moreover, the culture medium does not contain any glutamine. Since the feed culture medium contains serum-free protein-free animal-source-free components, the viral pollution risk can be greatly reduced, and downstream purification is facilitated. Meanwhile, since the culture medium is full in types of formula components and balanced in proportion, the culture medium can be applicable to high-density culture and high-protein expression of multiple CHO cell strains.

A natural rubber latex which is substantially free from proteins specified respectively by bands at 14, 31 and 45 kDa in the SDS-PAGE method; and a process for producing the natural rubber latex as described above which comprises saponifying a natural rubber latex with an alkali hydroxide in the presence of a surfactant. Because of substantially being free from the above proteins causative of the expression of type I allergy, the above-described natural rubber latex is appropriately usable in producing various products such as catheters, rubber gloves, condoms and foamed articles.

The invention relates to preparation of a cell culture medium, and particularly provides preparation of a novel serum-free protein-free defined-chemical-component cell culture medium. The culture medium contains multiple amino acids, vitamins, inorganic salts, minor elements, carbohydrates and other supplementary factors. The culture medium is free of any extract or hydrolysate. The culture medium is free of any animal-derived component. The culture medium can well support in-vitro suspension culture of CHO (Chinese hamster ovary) cells, and satisfies the demands for CHO cell culture. The culture medium contains defined chemical components, and is low in cost.

The objective of the invention is to provide amino acid formula powder and preparing method thereof. Effective components of the amino acid formula powder are prepared from, by mass, 15-35% of refined vegetable oil, 30-50% of maltodextrin, 12-20% of compound amino acids, 0.07-0.5% of docosahexoenoic acid (DHA), 6-14% of medium-chain fatty acids, 0.1-1.8% of arachidonic acid (ARA), 0.01-0.1% of vitamins, 1.2-2.4% of mineral compounds, 1-5% of fructooligosaccharide (Fos), 1-10% of galacto-oligosaccharides (Gos), 0.03-1.0% of taurine, 0.05-0.5% of L-carnitine and 0.038-0.240% of choline. Adoption of protein-free formula powder is of great significance for prevention and treatment of infant protein allergy. The amino acids, the vitamins, polysaccharides, and the like are adopted as supplements to form the novel amino acid formula powder, thus overcoming the nutrition issue of protein-allergic infants. The amino acid formula powder is suitable for protein-allergic infants. The objective is realized.