30 results about "Endotoxin Contamination" patented technology

The present invention includes recombinant proteins derived from Clostridium botulinum toxins. In particular, soluble recombinant Clostridium botulinum type A, type B and type E toxin proteins are provided. Methods which allow for the isolation of recombinant proteins free of significant endotoxin contamination are provided. The soluble, endotoxin-free recombinant proteins are used as immunogens for the production of vaccines and antitoxins. These vaccines and antitoxins are useful in the treatment of humans and other animals at risk of intoxication with clostridial toxin.

The present invention relates to methods and systems for the continuous analysis (160) and removal (410) of toxins from tobacco. Products, such as tobacco contaminated with mycotoxins, especially aflatoxins and benzopyrene and their precursors, are treated, usually in a solvent medium, to remove the toxin contamination from the tobacco. As an example, all noxious toxins eluted from the wash solvent (150) are continuously monitored (160) by immunoantibody UV fluorescence analysis. A quality control process ensures that harmful toxins are removed from the tobacco before further processing. Purification of extraction solvent streams and readditives ensures safe reuse or disposal of solvents or readditives.

The invention provides a process for the purification recombinantly expressed, self-assembled VLP from the homogenate of a bacterial host, wherein the process can be scaled up to a commercial production scale in a cost effective manner. The process comprises a first chromatography using an anion exchange matrix, a second chromatography using hydroxyapatite and, optionally, a size exclusion chromatography. VLP preparations obtained by the process of the invention are essentially free of endotoxin contaminations.

The present invention provides a method of biosynthesizing a recombinant protein containing the biologically active peptide, the pharmaceutical composition containing the recombinant protein as well as the preparation of the recombinant protein. Simply speaking the consecutively multiple copies of the bioactive peptide are replaced in the amino acid sequence of a recombinant protein (so called peptide-protein). Then, a high concentration and high yield of the peptide-protein containing the consecutively multiple copies of the bioactive peptide is produced by the biosafety and edible strain of yeast without any endotoxin contamination.

Methods for purifying fucoidan in extracts from brown seaweed are disclosed. In particular, methods of purifying fucoidan in the extract to remove heavy metal ions, bacterial and endotoxin contaminants, and other impurities are disclosed. The methods include the use of a chelating agent, selective precipitation, and filtration.

The present invention discloses a DTE immobilized nano microsphere and a preparation method thereof, and a method for producing D-psicose based on the DTE immobilized nano microsphere. Two geneticallyengineered bacteria are constructed in the method for one-step synthesis of the DTE immobilized nano microsphere. Firstly, a D-tagatose-3-epimerase gene (dte) is fused to a 5' end of a PHA synthetasegene (phaC) through a fragment of linker by a gene fusion method to obtain a dte-linker-phaC recombination gene fragment, the gene fragment is respectively transformed into food-grade expression hosts of L.lactis NZ9000 and non-endotoxin-contaminated ClearColi for inducible expression, and a PDE nano microsphere with DTE on surface can be synthesized in recombinant L. lactis or recombinant ClearColi cells, so that the DTE is effectively bonded to the surface of the nano microsphere, forming the immobilized DTE and achieving DTE production and immobilization in one step.

The present invention relates to a membrane based assay method, device and kit for rapid detection and / or quantification of endotoxins in aqueous solutions and test samples. The kit as per the present invention comprises lipopolysaccharide (LPS) affinity ligand conjugated with gold nanoparticles (GNPs); a membrane device comprising an endotoxin affinity membrane positioned parallelly to one or more layer(s) of a hydrophilic material, which are optionally secured in an enclosure; and optionally comprising an indicator chart for quantification of endotoxins in the sample. The method comprises placing the sample suspected of endotoxin contamination on a surface of a membrane comprised in a membrane device; placing once or more a suspension of LPS-affinity ligand conjugated with GNPs over the same area as the sample placed and detecting the presence of endotoxin if the colour signal appears and based on its intensity quantifying the endotoxin levels.

The invention provides a porcine circovirus type 3 Cap protein, nucleic acid, virus-like particles, vaccines and preparation methods and applications, and relates to the technical field of molecular biology. The porcine circovirus type 3 Cap protein provided by the invention has such as SEQ The protein with the amino acid sequence shown in ID NO.2 can be expressed solublely in a yeast expression system, and effectively improves the yeast expression efficiency of porcine circovirus type 3 Cap protein. Moreover, the porcine circovirus type 3 Cap protein provided by the present invention can be self-assembled into virus-like particles, and the vaccine prepared by using the virus-like particles has the characteristics of both cellular immunity and humoral immunity. Immunological related experiments show that the immune effect is very good. it is good. The use of yeast expression system to express porcine circovirus type 3 Cap protein has the advantages of low production cost, simple production process, a large amount of target protein can be obtained through high-density fermentation, and no endotoxin pollution, and can be used for large-scale production.

Methods for purifying fucoidan in extracts from brown seaweed are disclosed. In particular, methods of purifying fucoidan in the extract to remove heavy metal ions, bacterial and endotoxin contaminants, and other impurities are disclosed. The methods include the use of a chelating agent, selective precipitation, and filtration.