Porcine circovirus type 3 cap protein, nucleic acid, virus-like particle, vaccine, preparation method and application

Abstract

The invention provides a porcine circovirus type 3 Cap protein, nucleic acid, virus-like particles, vaccines and preparation methods and applications, and relates to the technical field of molecular biology. The porcine circovirus type 3 Cap protein provided by the invention has such as SEQ The protein with the amino acid sequence shown in ID NO.2 can be expressed solublely in a yeast expression system, and effectively improves the yeast expression efficiency of porcine circovirus type 3 Cap protein. Moreover, the porcine circovirus type 3 Cap protein provided by the present invention can be self-assembled into virus-like particles, and the vaccine prepared by using the virus-like particles has the characteristics of both cellular immunity and humoral immunity. Immunological related experiments show that the immune effect is very good. it is good. The use of yeast expression system to express porcine circovirus type 3 Cap protein has the advantages of low production cost, simple production process, a large amount of target protein can be obtained through high-density fermentation, and no endotoxin pollution, and can be used for large-scale production.

Description

Porcine circovirus type 3 Cap protein, nucleic acid, virus-like particle, vaccine and preparation method and application technical field The present invention relates to molecular biology technical field, relate in particular to a kind of porcine circovirus type 3 Cap protein, nucleic acid, Virus-like particle, vaccine and preparation method and application. Background technique Porcine circovirus (PCV) is a single-stranded circular DNA virus with a gene length of about 1.7kb, which is the smallest animal DNA disease One of the poisons, mainly causing multisystem collapse syndrome, pneumonia, dermatitis, nephrotic syndrome, reproductive disorders in piglets, Colonization brought heavy losses. PCV3 is a new type of circovirus, and there is no commercial vaccine against PCV3 in China. PCV3 and PCV2 The homology of the cap protein gene is very low, only 30% identical, which also implies that the existing PCV2 vaccine cannot protect pigs against immune Vulnerable t...

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Problems solved by technology

However, the PCVcap protein expressed by Escherichia coli has low solubility and is polluted by endotoxin, and the endotoxin removal process in the later stage is complex and expensive; the VLP expressed by the insect baculovirus system has a good immune effect, but the insect baculovirus produced VLP expression is low, production cost is expensive, the production process is complicated, and it is difficult to produce large-scale production as an animal vaccine

As an efficient protein expression system, the yeast expression system has many advantages, but due to the influence of the PCV3 cap protein's own nuclear localization sequence, it is easy to form insoluble protein aggregation when directly expressing the protein, so the soluble expression of the PCV3 cap protein in the yeast system challenging

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