242 results about "Metallothionein" patented technology

The invention relates to a fluorescent probe, wherein, the fluorescence is enhanced in the presence of ONOO-, and the fluorescence returns to original state in the presence of reducing matter. The invention provides a reversible fluorescent probe for selective detection of ONOO- in cells, characterized in that: cyanine dye is used as the fluorescent matrix, an organic selenide structure is introduced in the cyanine matrix as the active site of the reaction with ONOO- to realize the selective detection of ONOO-; the properties that organic selenium oxide formed by oxidizing organic selenide is easy to be reduced to organic selenide by reducing micromolecules in biosystem, such as cysteine, reducing glutathione, metallothionein and the like, is utilized to realize the reversibility of the probe molecules; and variations of electronic properties of organic selenide before and after the oxidation and the influence of the variations on the fluorescence property of the whole compound are utilized simultaneously to modulate the fluorescence property of the probe molecules.

Metallothionein is a stress response protein responsible for some forms of stress-related immunosuppression. An anti-metallothionein antibody can stimulate the humoral immune response to T-dependent antigens. An anti-metallothionein antibody is particularly useful when administered with an antigen to stimulate the immune response in a subject. An anti-metallothionein antibody can be administered to a subject with a vaccine to immunize the subject.

The invention belongs to the soil environment management field in the environmental engineering and relates to the construction and application of a new genetically engineered microorganism capable of restoring the soil polluted by heavy metal cadmium. The preparation method of the genetically engineered microorganism comprises the following steps: monkey metallothionein alpha structure domain tetramer is fused in the ice-nucleating protein N-end of Xanthomona campestris ATCC33913 to be used as a main functional unit for constructing a broad host range expression plasmid; then cadmium-specific promoter is used for expression in Pseudomonas putida X4 which can resist various heavy metals efficiently, thus realizing the surface display of monkey metallothionein alpha structure domain tetramer in the bacterium and obtaining genetically engineered microorganism CH01 which can adsorb heavy metal cadmium efficiently and specifically. The engineered microorganism can remarkably adsorb heavy metal cadmium in the presence of various heavy metals; and the result of the symbiotic water culture with wheat, cowpea and corn shows that the engineered microorganism CH01 can colonize in the rhizosphere of plant and fast adsorb cadmium ions. The invention also discloses a construction method of the genetically engineered microorganism CH01 of which preservation number is CGMCC No.M209320.

The invention relates to a liposome sun-screening skin care product, belonging to the technical field of cosmetics. The liposome sun-screening skin care product comprises the following components in parts by weight: 0.5-8.0 parts of epidermal growth factor (EGF), 0.01-0.1 part of human stem cell growth factor (SCGF), 10.0-10,000.0 parts of hyaluronic acid, 0.003-3000.0 parts of phospholipid, 0.001-10.0 parts of metallothionein, 0.001-10.0 parts of collagen, 10.0-5000.0 parts of water-soluble pearl powder the weight percentage of which is 20%, and 0.001-200.0 parts of cholesterol. The sun-screening skin care product provided by the invention has the beneficial effects that the liposome is composed of phospholipid bilayers and contains pure water micro vesicles, and the vesicles can enclose a certain amount of nutrients and essences, are 100 nanometers in mean diameter which is 1/200-1/300 of the human cell diameter, can easily penetrate through the surface layer of human skin and can be fused with cells, thus exerting efficacy. The liposome sun-screening skin care product can deeply humidify horny cells and enhance hydration to improve the skin softness, and can provide sufficient nutrients to ensure skin health and increase skin resistance.

The invention discloses an anti-stress fodder adjusting control agent for milk cows and a fodder containing the adjusting control agent. The fodder adjusting control agent is prepared from a pluralityof Chinese herbal raw materials, metallothionein course extract powder and alfalfa powder according to a certain proportion; and the fodder is prepared from the adjusting control agent and conventional fodder raw materials according to a certain proportion. A preparation method comprises the following steps: firstly, crushing various raw materials; secondly, adding the crushed components into solutions in a certain amount, dissolving the components, filtering the solutions, concentrating the filtrates and drying the concentrated filtrates, and obtaining extracts by separation; and finally, mixing the extracts, adding corn flour, soy bean meal and grass meal in certain proportion into the mixed extract, evenly stirring the mixture, and packing the mixture into a sealed bag for later use. The anti-stress adjusting control fodder for the milk cows has the advantages of reasonable formulation, wide raw material sources, low cost, feasible method and simple and convenient operation, avoidsadding any antibiotics or chemical medicine, has no residue or pollution, can effectively relieve the stress of the milk cows, improve the physiological function of the milk cows, particularly lactating cows, enhance the constitutions of the milk cows and improve the lactating amount of the milk cows and the quality of milk, and is particularly suitable for large-scale milk production.

The invention provides a preparation method of soluble recombinant sinopotamon metallothionein (rcMT), and the preparation method has the advantages of simplicity and convenience in operation, low cost, high purity and good activity. The method comprises the following steps: firstly constructing a phoA-MT recombinant expression vector by taking a phoA plasmid as an expression vector; further transforming escherichia coli BL21, constructing an escherichia coli engineering strain phoA-MT/BL21, and inoculating the engineering strain into an LB (Luria-Bertani) culture medium to prepare seeds, then diluting bacteria to a low-phosphorus culture medium for induction and expression, and utilizing Ni sepharoseTM6 Fast Flow affinity chromatography for purification so as to get the recombinant sinopotamon metallothionein. An anti-sinopotamon metallothionein monoclonal antibody prepared by utilizing the recombinant sinopotamon metallothionein has high sensitivity and strong specificity and can be applied to ELISA (enzyme-linked immunosorbent assay) quantitative assay of the metallothionein.

The invention discloses an anti-acne composition containing epidermal growth factors and tea tree oil and a preparation method thereof. The anti-acne composition disclosed by the invention comprises the following components: epidermal growth factors, tea tree oil, metallothionein, chlorhexidine, humectant, tackifier, alpha-carboxylic acid, pH value regulator, surfactant and deionized water. The preparation method of the anti-acne composition disclosed by the invention comprises the following steps of: mixing and heating the epidermal growth factors, the tea tree oil, the metallothionein, the chlorhexidine and the surfactant to be used as an oil phase; dissolving and heating the humectant, the tackifier and the alpha-carboxylic acid in water to be used as a water phase; adding the oil phase into the water phase, and stirring uniformly; and adding the pH value regulator for regulating the pH value, thereby obtaining the anti-acne composition containing epidermal growth factors and tea tree oil. The epidermal growth factors contained in the anti-acne composition disclosed by the invention can accelerate the metabolism of skin cells, and the natural tea tree oil capable of resisting bacteria and diminishing inflammation can efficiently sterilize bacteria, diminish inflammation and remove acnes, thus the anti-acne composition has good moisture retention and small skin irritation.

The invention discloses a magnetic biological material capable of adsorbing uranium (VI) in an aqueous solution and a preparation method of the magnetic biological material. The magnetic biological material has the particular name of gene recombinant saccharomyces cerevisiae microsphere, which is obtained by mixing human liver metallothionein gene recombinant saccharomyces cerevisiae with a ferromagnetic fluid and immobilizing by sodium alginate and calcium chloride; wherein the mixing ratio is that the mass ratio of gene recombinant saccharomyces cerevisiae bacteria to the ferromagnetic fluid is 10:1. The magnetic gene recombinant saccharomyces cerevisiae microsphere has such characteristics of gene recombinant saccharomyces cerevisiae as high uranium (VI) adsorption capacity, low adsorption cost, recyclable biomass and no secondary pollution, and is relatively convenient and rapid to recycle, thereby greatly enhancing the recycling ratio, reducing the uranium pollution degree, and meanwhile promoting the uranium resource recycling actively.

The invention relates to a method for inducing and extracting metallothionein from housefly larva, which comprises the following steps of: culturing 3-5-day housefly larva in a nonmetal culture dish by using a culture medium of a metal ion biological inductive agent of Cd2+, Zn2+ and Cu2+; and carrying out rinsing, sterilizing, low temperature and high speed homogenate, membrane filtration, chromatographic fractionation, elution and concentration, and vacuum freezing and drying on the housefly larva to obtain Zn/Cu metallothionein which has molecular weight of 5300-6500 dDa and contains 20-27 percent of cysteine and subtypes thereof, wherein the molecular structure of the Zn/Cu metallothionein takes less aromatic amino acids. The yield of the metallothionein in biologically induced larva protein can reach 1mg/g. The production method has low cost, high purity and high yield of metallothionein.

The invention relates to the field of controlled release of biomedical materials and drugs, and particularly relates to a nanoparticle preparation, and a preparation method and application thereof. The nanoparticle preparation is prepared from the following components: (1) oligopeptides and/or oligopeptides derivatives; and (2) metal ions. The nanoparticle preparation is uniform and controllable in particle size, high in drug loading ratio, and good in stability in blood, can quickly release drugs via multiple stimulated response of pH/enzyme/glutathione/metallothionein, and is easy in preparation method and easy for large-scale production.