50 results about "Sclareol" patented technology

The present invention provides a method of producing sclareol, said method comprising a) contacting a particular polypeptide having a LPP synthase activity with GGPP and b) contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP) produced in step a). In particular, said method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptides used in the method. Nucleic acids derived from Salvia sclarea and encoding the polypeptides of the invention, expression vectors containing said nucleic acids, as well as a non-human host organism or a cell transformed to harbor the same nucleic acids, are also part of the present invention.

The present invention provides a method of producing sclareol, the method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, the method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing the nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.

The invention relates to a preparation method of sclareolide, comprising the following steps of: (a). oxidizing sclareol to obtain a crude sclareolide product; and (b) crystallizing the crude sclareolide product obtained in the step (a) to obtain a pure sclareolide product. The preparation method is characterized in that sclareolide is recovered and processed from crystallization mother liquor obtained in the step (b), and the obtained sclareolide is mergered into the step (b) and crystallized and purified together with the synthesized crude sclareolide product. The invention adopts the environment-friendly method with simple operation, realizes the purpose of recovering sclareolide from the sclareolide crystallization mother liquor, improves the total recovery of the sclareolide and establishes a solid foundation for improving the commercial value of the sclareolide.

The invention relates to the technical field of natural perfume separation, in particular to a separation method of sclareol. The invention overcomes the problems in the prior art that the production efficiency is low, the yield is low, the energy consumption is great and the cost is high. Aiming at overcoming the problems in the prior art, the technical proposal of the invention is as follows: the separation method of the sclareol comprises the following steps sequentially: (1) preliminary treatment: the crude extract of sage clary is heated to be fully melted, and centrifugal extraction is carried out to remove heavy phase substances; (2) primary stage of distillation: the crude extract of the sage clary is sent to a molecular still, after continuous distillation, the extract is dividedinto heavy phase and light phase, wherein, the light phase is carried out with secondary distillation; and (3) secondary distillation: the light phase obtained in the step (2) is sent to the molecular still again to obtain the sclareol with the concentration of 90 to 98 percent. Compared with the prior art, the method has the advantages of high production efficiency, high yield reaching more than95 percent, low energy consumption and low cost.