127 results about "Cryptococcus" patented technology

The invention discloses Cryptococcus capsules and a method for preparing the same. The method for preparing the Cryptococcus capsules comprises the step of mixing a solution of alginate, Cryptococcus, multivalence metal cations to obtain the Cryptococcus capsules. Experiments show that the viable bacterium rates of the Cryptococcus capsules prepared by the method are all above 70 percent; the highest viable bacterium rate is up to 90 percent; and the activity of bacteria is not reduced. The Cryptococcus capsules of the invention can be stored at both low and normal temperature, the effect of storage for one month at normal temperature is substantially the same as that of the storage at low temperature and the biological activity of the bacteria are not influenced. Therefore, the method has wide application prospect in the preparation of biological agents and the preservation of other organisms.

The invention relates to a triple nucleic acid test kit. The kit comprises a thallus lysate, a positive quantitative reaction liquid, a negative control reaction liquid and a thallus test reaction liquid, wherein the thallus test reaction liquid contains specific primers, specific probes and doping fluorescent dye for aspergillus, Cryptococcus neoformans and candida albicans, the three specific oprimers are designed according to areas of ITS sequences of aspergillus, Cryptococcus neoformans and candida albicans, FAM, VIC and CY-5 are selected for mark report groups of the three specific probes, MGB serves as a quenching group, the excitation wavelength of the FAM group is 492 nm, and the emission wavelength is 517 nm; the excitation wavelength of the VIC group is 557 nm and the emission wavelength is 574 nm; the excitation wavelength of the CY-5 group is 646 nm and the emission wavelength is 664 nm. The triple nucleic acid test kit for aspergillus, Cryptococcus neoformans and candida albicans can assist in diagnosing infection caused by aspergillus, Cryptococcus neoformans and candida albicans.

The invention relates to the fungal polysaccharide preparation field, and concretely relates to a preparation method of Cryptococcus Neoformans capsular polysaccharide. The invention provides a method for preparing the Cryptococcus Neoformans capsular polysaccharide through utilizing cetyl trimethyl ammonium bromide. The method comprises the following steps: 1, processing a secretion supernatant containing the capsular polysaccharide through using calcium acetate and glacial acetic acid, and precipitating through using ethanol to prepare a crude capsular polysaccharide; and 2, processing a solution of the crude capsular polysaccharide through using the cetyl trimethyl ammonium bromide to obtain the purified capsular polysaccharide. The method has the advantages of simplicity, high efficiency, low cost, and effective avoiding of the use of toxic and harmful chemical reagents.

Disclosed are a preparation method and application of Anaphalis lactea essential oil. Volatile oil in Anaphalis lacteal is extracted by steam distillation and CO2 supercritical extraction, ingredients of the volatile oil are analyzed initially by gas chromatography/mass spectrography, 57 ingredients in the volatile oil are identified and account for more than 94% of the total volatile oil. Antibacterial and bactericidal activities of the volatile oil are researched by the Oxford cup method, and according to the research, the volatile oil has an evident killing or inhibiting function for bacteria, such as staphylococcus, streptococcus, enterobacter, salmonella, shigella, pseudomonas, bacillus, escherichia coli and the like, and fungi, such as candida, cryptococcus, penicillin, aspergillus, mucor, microsporum, trichophyton, epidermophyton and the like. Testing by DPPH (diphenylpicrylhydrazyl) method shows that the volatile oil has fine antioxidant activity. Owing to the fine activity, the volatile oil is widely applicable to the industries of foods, medicines, cosmetics, health-care products and the like.

The invention discloses a calcineurin catalytic subunit gene which is cloned in a cryptococcus humicolus BSLL1-1 strain. The nucleotide sequence of the calcineurin catalytic subunit gene is shown in SEQ ID NO:1 and is used for coding the protein with an amino acid sequence shown in SEQ ID NO:2; the protein is expressed in escherichia coli, and the calcineurin catalytic subunit gene is further successfully expressed in saccharomyces cerevisiae. According to the calcineurin catalytic subunit gene, the saccharomyces cerevisiae is enhanced in aluminum resistant capacity, genetically engineered bacteria are capable of adsorbing (or absorbing) activated aluminum contained in a culture medium, and the genetically engineered bacteria of the saccharomyces cerevisiae have application prospect of reducing the content of activated aluminum contained in acid soil.

The invention relates herba dracocephalum heterophylli volatile oil possessing the bacteriostatic and germicidal action and its application. The method adopts steam distillation to extract the herba dracocephalum heterophylli volatile oil and adopts gas-chromatography/mass spectra to analysis the volatile oil, identifying the 83 components and 89.83% total content. The volatile oil can kill and suppress the staphylococcus, streptococcus, gonococcus, enterobacter, Escherichia, salmonella, shigella, pseudomonas, bacillus, oidiomycin, cryptococcus, aspergillus, penicillium, mucor, epidermophytin, micromonospora, trichophyton and other bacteriums. The herba dracocephalum heterophylli volatile oil is used for foodstuffs, drugs, health products, cosmetic products and other industries.

The invention relates to staining fluid and a method for rapidly detecting novel Cryptococcus. The staining fluid comprises 0.5-3.0g of AZO-blue, 1.0-10.0g of glacial acetic acid and 90-110g of distilled water. The method for rapidly detecting novel cryptococcus comprises the steps of preparation of the staining fluid, mixing, detection, counting and the like. The invention has the advantages of simple, convenient, rapid, sensitive and accurate detection, high specificity, easy preservation of the staining fluid, short staining time and excellent staining effect.

The invention discloses a primer, molecular beacon and kit for fast detecting various clinically common fungi and identifying strains and belongs to the technical field of microbiological detection. The sequences of the primer is as shown in SEQ ID No. 1-4, and the sequences of the detecting molecular beacon is as shown in SEQ ID No. 5-7. The primer and the molecular beacon have the advantages that the specific primer and the molecular beacon are designed at conserved sites by comparing the gene sequences of various clinically common fungi, and the primer and the molecular beacon can fast detect and identify 8 clinically common candida, 1 cryptococcus, aspergillus, mucor and penicillium. The kit for detecting various fungi contains the primer and the molecular beacon and can fast and accurately detect various clinically common fungi.

The compositions and methods described herein discloses the design, synthesis and testing of compounds that act as inhibitors of DHFR. The basic scaffold of these inhibitors includes a 2,4-diaminopyrimidine ring with a propargyl linker to another substituted aryl, bicyclo or heteroaryl ring. These DHFR inhibitors are potent and selective for many different pathogenic organisms, including the DHFR enzyme from bacteria such as Bacillus anthracis and methicillin-resistant Staphylococcus aureus, fungi such as Candida glabrata, Candida albicans and Cryptococcus neoformans and protozoa such as Cryptosporidium hominis and Toxoplasma gondii. These compounds and other similar compounds are also potent against the mammalian enzyme and may be useful as anti-cancer therapeutics.

The invention provides an oligosaccharide vaccine for preventing invasive fungal infection. The oligosaccharide vaccine comprises chitosan oligosaccharide coupled with an immunogenic carrier protein;the mass ratio of the immunogenic carrier protein to the chitosan oligosaccharide is 0.7-210: 1; the chitosan oligosaccharide has a deacetylation degree of 0.05-98% and an average relative molecular weight smaller than 5000 Da; and the immunogenic carrier protein is a non-human protein. The oligosaccharide vaccine provided by the invention is capable of effectively activating Th17 and Th1 cellularimmunity through a proper acetylation degree so as to prevent broad-spectrum fungal infections. The oligosaccharide vaccine is capable of treating or preventing invasive fungi such as candida albicans, aspergillus, cryptococcus and pneumocystis jeroveci.

It is intended to provide novel compositions usable in improving the flavor of an alcoholic beverage made from grapes typified by wine. Namely, a composition usable in improving the flavor of an alcoholic beverage made from grapes which contains the culture of a strain belonging to a genus Aspergillus, Penicillium, Rhizopus, Rhizomucor, Talaromyces, Mortierella, Cryptococcus, Microbacterium, Corynebacterium or Actinoplanes and being capable of producing diglycosidase.

The invention relates to a soil-environment microorganism disinfectant and a preparing method thereof. The disinfectant is a liquid fertilizer type soil disinfectant prepared from inorganic nutrient substances and microorganism cenobium capable of removing soil toxins in a combined mode. According to the disinfectant and the preparing method, five kinds of bacteria which have bacterial strains such as cryptococcus humicolus, lactobacillus acidophilus, coralline Nocardia amarae, actinomycetes agrobacterium tumefaeiens and trichoderma koningii and have ingredients capable of powerfully removing toxic pollutants in soil are screened out; test surveying indicates that after the microorganism disinfectant is applied, 85% to 90% of remaining hazardous substances such as pesticides can disappear in 30 days to 50 days, and the remaining hazardous substances can be completely removed after the microorganism disinfectant is continuously applied several times.

The invention discloses a cryptococcus detection card. The card comprises a rubber plate, wherein a sample pad, a gold mark pad, a chromatographic film and a first water absorption pad are sequentially arranged on the rubber plate end to end along the sample flowing direction; a second water absorption pad is arranged between the chromatographic film and the rubber plate; a detection area and a quality control area are arranged on the chromatographic film; and a colloidal gold marked first monoclonal antibody for resisting cryptococcus capsular polysaccharide is adsorbed on the gold mark pad.The detection card or detection box can be adopted to quickly and sensitively detect the existence of cryptococcus capsular polysaccharide antigen, and determine whether a detected object is affectedby cryptococcus or not. The detection card or detection box has high sensitivity, strong specificity and high detection speed, can give a detection result in five minutes, and is simple in operation.

The invention relates to a method for biologically synthesizing sclareolide. The method comprises the steps of taking a yeast culture medium and separately adding sclareol and a light white cryptococcus protoplast fluid into the yeast culture medium to obtain a mixture, wherein the sclareol accounts for 3-8% of the total mass of the yeast culture medium, and the light white cryptococcus protoplastfluid accounts for 4-10% of the total mass of the yeast culture medium; placing the mixture in a glass vessel, and then shaking the vessel for fermentation for 10 days on a shaking table at the temperature of 23-28 DEG C and the speed of 140-180 r/min to obtain sclareolide. According to the method, a microorganism conversion method is utilized, and sclareol is used as a substrate and converted bylight white cryptococcus to prepare sclareolide, so that the problems that a synthesis technology process of sclareolide based on a chemical method is environmentally unfriendly, complicated in step,low in safety and the like are solved, and a new way is provided for synthesizing sclareolide.

The invention discloses a C2H2-type transcription factor gene asr1. The C2H2-type transcription factor gene asr1 is cloned in a Cryptococcus humicolus BSLL1-1 strain, has a nucleotide sequence shown in the formula of SEQ ID NO: 1 and codes a protein with an amino acid sequence shown in the formula of SEQ ID NO: 2. An asr1 recombinant fragment blocked by a hygromycin gene is introduced into Cryptococcus humicolus, the Cryptococcus humicolus produces mutation, and the mutant strain increases resistance to various ionic stresses and has the application potential to reduce various ions in soil.

The invention discloses a microbial preparation efficiently converting heavy metal cadmium in contaminated soil and a preparation method thereof. The microbial preparation is obtained by mixing and conducting enrichment cultivation on a flora 1 composed of candida, phialemonium, rhodosporidium toruloides and cryptococcus and a flora 2 composed of acidithiobacillu thiooxidans, acidithiobacillus caldus, leptospirum ferrooxidans, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and ferroplasma thermophilum. By using the microbial preparation for treating the cadmium contaminated soil, after the metal cadmium is converted to be a soluble state from an insoluble state, the removal rate is 70-82%, and the microbial preparation has the advantages of being high in conversion efficiency, short in cycle, low in cost, free of secondary pollution, easy to popularize and the like.

The invention discloses an application of a microbial preparation for efficiently converting heavy metal cadmium in polluted soil. The microbial preparation is prepared by performing enriched cultivation on the following two mixed floras: (1) candida mycoderma bacteria, aspergillus monicus, rhodosporidium toruloides and cryptococcus which form a flora 1; and (2) acidithiobacillus thiooxidans, leptospirillum ferrooxidans, acidithiobacillus caldus, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and thermos ferric bacteria which form a flora 2. By using the microbial preparation to treat the cadmium polluted soil, the metal cadmium is converted from being indissolvable into being soluble and the removal rate is 70-82%. The microbial preparation disclosed by the invention has the advantages of being high in conversion efficiency, short in period, low in cost, free of secondary pollution, easy to popularize and the like.