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Cryptococcus bacteria capsule and preparation thereof

The technology of Cryptococcus and capsule is applied in the field of Cryptococcus capsule and its preparation, can solve the problems of high cost, large energy consumption and the like, and achieve the effects of simplifying operation, saving resources, and facilitating transportation and sales

Inactive Publication Date: 2009-08-12
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in commercial production, freeze-drying requires large-scale equipment, and consumes a lot of energy and costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the preparation of the capsule of yeast antagonistic bacteria (Cryptococcus laurentii)

[0021] 1. Preservation, activation and fermentation of yeast antagonistic bacteria Cryptococcus laurentii

[0022] 1. Activation of strains

[0023] Take the yeast antagonist (Cryptococcus laurentii) CGMCC No.1029 (CN1733898) stored at -20°C, inoculate it in NYDA medium, plate it, and culture it at 28°C for 72 hours; after 72 hours, take a small amount of well-grown yeast antagonist Bacteria were inoculated in NYDB liquid medium for further activation culture; plated and streaked on NYDA medium to obtain a single colony.

[0024] NYDA medium: glucose 10, soybean peptone 5, beef extract 1, yeast extract 7.5, agar 18, unit: gL -1 .

[0025] NYDB liquid medium: glucose 10, soybean peptone 5, beef extract 1, yeast extract 7.5, unit: gL -1 .

[0026] Soybean peptone was purchased from Beijing Shuangxuan Microbial Medium Products Factory, the catalog number was 02-19; ​...

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PUM

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Abstract

The invention discloses Cryptococcus capsules and a method for preparing the same. The method for preparing the Cryptococcus capsules comprises the step of mixing a solution of alginate, Cryptococcus, multivalence metal cations to obtain the Cryptococcus capsules. Experiments show that the viable bacterium rates of the Cryptococcus capsules prepared by the method are all above 70 percent; the highest viable bacterium rate is up to 90 percent; and the activity of bacteria is not reduced. The Cryptococcus capsules of the invention can be stored at both low and normal temperature, the effect of storage for one month at normal temperature is substantially the same as that of the storage at low temperature and the biological activity of the bacteria are not influenced. Therefore, the method has wide application prospect in the preparation of biological agents and the preservation of other organisms.

Description

technical field [0001] The invention relates to a cryptococcus capsule and a preparation method thereof. Background technique [0002] A major problem in the commercial production of biocontrol agents is the need to find a suitable method to maintain sufficient biocontrol efficacy during the shelf life of the product. Freeze-drying is a successful and effective dosage form processing method, and the formed dry powder preparation is conducive to preservation and sales. However, in commercial production, freeze-drying requires large-scale equipment, and consumes a lot of energy and high cost. In order to reduce production costs and improve biocontrol efficacy, it is necessary to find other dosage form processing methods. [0003] Alginic acid is a polysaccharide in the cell wall components of brown algae. It is a polyuronic acid composed of D-mannuronic acid and L-guluronic acid. It is found in marine organisms such as copper algae, wakame, kelp and kelp. Rich in content. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/04C12R1/645
Inventor 田世平李博强秦国政徐勇孟祥红
Owner INST OF BOTANY CHINESE ACAD OF SCI
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