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Colloidal gold immunochromatographic test strip and preparation method and application thereof

A technology of immunochromatographic test paper and colloidal gold, which is applied in the direction of analyzing materials, measuring devices, instruments, etc., can solve the problems such as no detection method for cryptococcus

Pending Publication Date: 2018-03-06
DYNAMIKER BIOTECH TIANJIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically, the present invention relates to a kind of mouse IgG type monoclonal antibody BE-A6 and anti-Cryptococcus neoformans capsular polysaccharide GXM antigen for Cryptococcus neoformans capsular polysaccharide (Glucuronoxylomannan, GXM) antigen. The preparation method and its application in the detection of Cryptococcus neoformans antigen, the patent only provides the preparation method of Cryptococcus monoclonal antibody, and does not provide the detection method of Cryptococcus

Method used

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  • Colloidal gold immunochromatographic test strip and preparation method and application thereof
  • Colloidal gold immunochromatographic test strip and preparation method and application thereof
  • Colloidal gold immunochromatographic test strip and preparation method and application thereof

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Embodiment 1

[0080] The preparation of embodiment 1 colloidal gold immunochromatography test strip

[0081] Colloidal gold immunochromatography test strip of the present invention can be prepared according to the following method, specifically refers to the structure such as figure 1 Shown:

[0082] Paste the sample pad, colloidal gold pad, nitrocellulose membrane, and water-absorbing filter paper on one end of the polyvinyl chloride (PVC) rubber sheet, and then cut the PVC rubber sheet and the attached material into a width of 4± 0.2mm test strip, and put it into the card case, which has a sample adding area and a color developing area (observation area).

[0083] Wherein, the sample pad is a glass fiber membrane; wherein the gold-labeled cryptococcal antibody with a concentration of 2-8 μg / mL is embedded on the colloidal gold pad; the nitrocellulose membrane includes: the blocking line is HAMA blocking The mixture of HAMA inhibitor and HBR blocking agent, the concentration of the HAMA ...

Embodiment 2

[0103] Embodiment 2: the preparation of colloidal gold immunochromatography test strip

[0104] Compared with Example 1, in the preparation of the colloidal gold pad: the separation agent shown is 0.1% casein.

Embodiment 3

[0105] Embodiment 3: the preparation of colloidal gold immunochromatography test strip

[0106] Compared with Example 1, in the preparation of the colloidal gold pad: the separation agent shown is 0.25% casein.

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Abstract

The invention relates to a colloidal gold immunochromatographic test strip and a preparation method and application thereof. The colloidal gold immunochromatographic test strip comprises a colloid strip. The colloid strip comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane and water-absorbing filter paper which are sequentially overlapped. A gold-labeled cryptococcal antibody is embedded in the colloidal gold pad, the nitrocellulose membrane is coated with a cryptococcal antibody as a detection line, is also coated with an antibody against a virus antibody on colloidal goldas a control line. The colloidal gold immunochromatographic test strip has good sensitivity, specificity, repeatability and stability, has a high recovery rate for a target compound, and can providemore accurate and reliable test results.

Description

technical field [0001] The invention belongs to the technical field of immunological detection and analysis, and relates to a colloidal gold immunochromatographic test strip and its preparation method and application, in particular to a colloidal gold immunochromatographic test strip for detecting cryptococcus. Background technique [0002] Cryptococcus belongs to the subphylum Deuteromycota, Bacillus, Cryptococcus, and Cryptococcus family in fungal taxonomy. The cryptococci that cause human infection are mainly Cryptococcus neoformans and Cryptococcus gattii. The asexual propagules of the two cryptococci are all hyphae-free monospore yeast-like bacteria, which have no capsule or only a small capsule in vitro, and soon form a thick capsule after entering the human body. Capsulated cryptococcus The diameter of the bacteria increased significantly, and the pathogenicity was significantly enhanced. [0003] Cryptococcus (Crytococcus) is an important opportunistic pathogen that...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/558G01N33/56911
Inventor 彭洁李昕彤李宁周泽奇粟艳
Owner DYNAMIKER BIOTECH TIANJIN
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