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Method for extracting and purifying sclareol from sclareol fermentation liquor

A technology of sclareol and fermentation liquid, applied in the separation/purification of hydroxyl compounds, organic chemistry, etc., to achieve the effect of simple operation, less process steps, and environmental friendliness

Active Publication Date: 2022-06-03
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for efficient extraction and purification of slavonol by creating an organic liquid that contains it during production process or use after manufacturing. It achieves this through simplified procedures, easy-to-use chemicals, and reused solvents.

Problems solved by technology

This patented technology describes how plants release volatile compounds called slavones during their process of photosynthesis (the breakdown of chlorophyll). These molecules help stimulate certain receptive cells like roots, flowers, fruits, bacteria, fungi, etc., which helps them grow better over time. However, these compound have potential harmful effects when consumed directly without being extracted beforehand due to its strong odor. Therefore, this new technique involves separating pure naturally occurring ones from unsanitary sources such as scorch flora.

Method used

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  • Method for extracting and purifying sclareol from sclareol fermentation liquor
  • Method for extracting and purifying sclareol from sclareol fermentation liquor
  • Method for extracting and purifying sclareol from sclareol fermentation liquor

Examples

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Effect test

Embodiment 1

[0024] 5g of dried bacterial powder was placed in 75mL of petroleum ether, extracted by microwave at 60°C for 10min, filtered with a suction filtration device, the filtrate was collected, and the filtrate was concentrated to obtain 0.74g of extract, with an extraction rate of 14.80%.

Embodiment 2

[0026] 5g of dried bacterial cell powder was placed in 75mL of n-hexane, extracted by microwave at 60°C for 10min, filtered with a suction filtration device, the filtrate was collected, and the filtrate was concentrated to obtain 0.67g of extract, with an extraction rate of 13.40%.

Embodiment 3

[0028] Put 5g of dry bacterial powder in 25mL of absolute ethanol, extract by microwave at 60°C for 10min, filter with suction filtration device, collect the filtrate, and concentrate the filtrate to obtain 0.78g of extract, with an extraction rate of 15.60%.

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Abstract

The invention discloses a method for extracting and purifying sclareol from sclareol fermentation liquor. The method comprises the following steps: (1) centrifuging fermentation liquor to obtain thalli, and drying and crushing the thalli; (2) extracting sclareol in the thalli by using an organic solvent under the assistance of microwaves to obtain an extract; and (3) extracting the extract with an acetone aqueous solution, and recrystallizing to obtain sclareol. After the fermentation liquor is extracted and purified by the method, the purity of the obtained sclareol can reach 95.11%. The recovery rate of sclareol in fermentation liquor can reach 63.95%. The extraction and purification process has few steps, the operation is simple, the used solvent can be recycled, and the method is environment-friendly.

Description

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Claims

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Application Information

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Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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