398results about How to "High extraction purity" patented technology

The invention provides a magnetic bead process nucleic acid extraction and transformation kit, and a using method thereof. The kit includes a lysate, a protease K, a magnetic bead solution, a transforamtion reagent, a nucleic acid protection agent, a binding solution, a washing solution I, a washing solution II and an eluent. The using method of the kit includes the following steps: (1) lysis; (2)transformation; (3) layering; (4) binding; (5) primary washing; (6) secondary washing; (7) drying; and 8) elution. The kit and the using method thereby can complete the transformation while achievingautomatic extraction of nucleic acid from a sample. The sample is lysed, releases the nucleic acid, and then begins to transform, the nucleic acid is separated from proteins by the binding solution after the transformation is completed, the methylated nucleic acid is purified by a magnetic bead process, and a pre-denaturation step in subsequent qPCR detection of the sample is used to replace a previously omitted sulfo group removal step, so a purification process in the original nucleic acid extraction step is omitted in the entire extraction and transformation process.

The invention relates to a preparation method of a gel containing stem cell exosomes for repairing skin wounds. The method comprises the following steps: 1) primary extraction and culture of human umbilical cord mesenchymal stem cells: 1.1) primary extraction of human umbilical cord mesenchymal stem cells, 1.2) subculture, and 1.3) collection of the culture supernatant; 2) extraction of human umbilical cord mesenchymal stem cell exosomes: 2.1) primary centrifugation, 2.2) secondary centrifugation, 2.3) removal of organelles by centrifugation, 2.4) coarse extraction of exosomes, and 2.5) finalextraction of exosomes; 3) preparation of a gel material: 3.1) preparation of chitosan, 3.2) configuration of beta-glycerol phosphate (beta-GP), and 3.3) preparation of the gel material; and 4) gel loading of the exosomes. The gel containing stem cell exosomes can promote repair of skin wounds, shorten the healing time of wounds and reduce scar formation.

The invention discloses a production method for separation and purification of salidroside from rhodiola rosea. The method comprises the following steps: after being smashed, raw materials are thrown in from a throwing hopper; a mainframe of an extraction tank set rotates; the materials are slowly propelled from the front end of the set to the back end; and at the same time, extraction solvent is introduced in the extraction tank from a liquid inlet pipe at the tail end of the set, and penetrates the movable materials from the back end of the tank to flow to the front end; and the solid-phase and liquid-phase materials are fully contacted with each other in the inverse movement so as to extract the effective components of medicinal materials. Medicine dregs are forcedly pushed to a dreg outlet through a discharge conveyer for discharging, and are extruded by using a special juice extruder to extrude residual medicine juice from medicinal material organizations, so that the content of the residual medicine juice of the medicine dregs is reduced, the extraction efficiency is higher, and the extraction is more thorough. The method chooses macroreticular resins and combines with the silica column chromatography to purify, so that better separation effect is achieved. Finished products of salidroside with the highest yield of 1% and the content above 95% can be obtained.

The inulin extracting process includes the following steps: washing, crushing, water leaching, filtering in a primary filter, a tubular filter and an ultrafiltering and decolorizing machine successively, nanometer filtering to concentrate, decolorizing with active carbon, secondary filtering, electrodialysis, evaporating concentration and spray drying. The process has high inulin product purity and yield, short extracting period, simple operation and low production cost.

Belonging to the field of green tea polyphenol extraction processes, the invention discloses a preparation process for extracting green tea polyphenols from tea. The preparation process includes the following six steps: pretreatment of tea, nitrogen protection, ultrasonic extraction, separation and filtration, resin adsorption, concentration and drying. The process provided by the invention solves the problems of serious destruction of effective components, low product yield and purity, high production cost and pollution in existing green tea polyphenol extraction. The preparation method disclosed in the invention not only has the advantages of high recovery rate, time saving, high extraction rate and the like, but also avoids the use of toxic solvents, and is conducive to reducing energy consumption and lowering the production cost, thus having good industrial promotion value.

The invention discloses a method for extracting isoflavone from kudzuvine root efficiently, and belongs to the technical field of the processing of agricultural products and food. The method comprises the following steps of: (1) crushing, namely crushing kudzuvine root to form 60 to 80-mesh kudzuvine root powder; (2) performing enzymolysis, namely adding 70 percent of ethanol into the kudzuvine root powder, adding cellulase and protease and performing the enzymolysis to obtain kudzuvine root extract, wherein 1 to 2 grams of the cellulase and the protease are added into each 100 milliliters of solution; and (3) extracting, namely performing ultrasonic-microwave processing on the obtained kudzuvine root extract from the step (2), concentrating under reduced pressure, freezing and drying to obtain kudzuvine root isoflavone. In the method, the kudzuvine root isoflavone is extracted by a compound enzymolysis technology of the cellulase and the protease and combining an ultrasonic-microwave assisted extraction technology, so the technical advantages of chemical enzymolysis and physical extraction are expressed fully, the cost is reduced, and the dissolution rate and extraction rate of the kudzuvine root isoflavone are improved, so that the extraction purity is greatly higher than that by conventional extraction method.

The invention discloses a technique for extracting a rare earth component from waste slag from the crystal industry by using a reselection technique, in particular a method for separating rare earth from other components of crystal waste slag. The technical process of the technique comprises: coarsely selecting and grading the crystal waste slag, pulping the graded crystal waste slag, performing reselection and sorting by using reselecting equipment, separating to obtain a heavy component which is high-content rare earth polishing powder waste and a sub-heavy component which is rare earth-containing coexisting component, performing ball-milling and reselection and sorting to obtain a high-rare-earth-content component, wherein the high-rare-earth-content component can be regenerated to produce rare earth polishing powder or be used in other fields, so that the recycling of solid waste is realized. The invention provides a method for easily, practically and reliably extracting a rare earth component from crystal waste slag, which belongs to a physical separation method and is an environment-friendly, high-efficiency and low-cost treatment method.

The invention discloses a method for preparing glucoraphanin from broccoli, which comprises the following steps: cleaning the raw material, chopping, adding into an extraction tank set host, and sufficiently contacting solid-phase and liquid-phase substances in the extraction tank set in a reverse motion process, thereby extracting effective components from the medicinal material. The medicine slag are forcedly pushed to a slag outlet to be discharged by a discharge conveyer, and a special squeezing machine extrudes the medicine slag to obtain medicinal material tissues from the residual liquor in the medicine slag, thereby reducing the residual liquor content in the medicine slag. The method has the advantages of higher extraction efficiency and more complete extraction. By adopting the macroporous adsorbent resin combined with silica gel column chromatography for purification, the separating effect is better. The method can be used for preparing the glucoraphanin finished product of which the maximum yield is 1% and the content is higher than 95%.

The invention belongs to the field of natural organic chemistry, and relates to a method for enriching agaric polysaccharide in purified black agaric by using macroporous absorption resin. The invention is characterized in that a macroporous absorption technology is applied to separate the purified black agaric polysaccharide, the absorption selectivity to the agaric polysaccharide is good, the absorption and analysis are both fast, and the absorption capacity is larger; and the extraction is convenient and fast, the raw material source is rich, the production is low, the separating effect is obvious, the extracting purity is high, semi-finished products of agaric polysaccharide with the content of more than 35 percent and final products of agaric polysaccharide with the content of more than 98 percent can be obtained, and the defects that the conventional method has lower extraction rate and low extraction purity are overcome. The method selects macroporous absorption resin with stable physical-chemical properties, larger surface area, faster exchange velocity, high mechanical strength, strong anti-pollution capability and good heat stability, can absorb the agaric polysaccharide selectively from solution by physical absorption, and has fast absorption and analysis and larger absorption capacity.

The invention relates to a fucoidan extract method, in particular to a method for extracting fucoidan for brown seaweed, comprising the following steps: 1. desalting: adopting fresh brown seaweed or unfreezed frozen brown seaweed to soak in pure water to remove salt; 2. enzymolysis: adding enzyme in the desalted brown seaweed to perform enzymolysis at 40-60 DEG C for 1-3h; 3. abstraction: adding calcium chloride in enzymatic hydrolyzate obtained by enzymolysis, and heating the solution to 90-100 DEG C for 1-4h to obtain the fucoidan extract. The invention is characterized of simple operation, high application security, high extraction efficiency, high extraction purity and the like and the extraction process is applicable to factory mass production.

The invention provides a method for extracting undenatured type II collagen from squid cartilage. The method includes: freeze-drying squid cartilage; crushing the freeze-dried squid cartilage, and sieving to obtain cartilage powder; suspending the cartilage powder in HCL solution, EDTA solution and NaOH solution to obtain precipitate; adding acetic acid solution and protease into the precipitate, stirring, centrifuging and collecting supernate; adding saturated NaCl solution into the supernate until NaCl concentration is 1-1.5M, allowing standing for 16-24h, centrifuging, and removing supernate to obtain first precipitate; dissolving the first precipitate by using acid, performing dialysis, and performing freeze-drying to obtain the undenatured type II collagen. The purity of the undenatured type II collagen acquired by extracting is not lower than 95%.

The invention discloses a purple sweet potato anthocyanin extraction process and relates to the technical field of plant extraction. The purple sweet potato anthocyanin extraction process comprises the following steps: (1) purple sweet potato pretreatment; (2) extraction: taking a mixed liquor comprising methyl alcohol, water and acetic acid according to the weight ratio of methyl alcohol to water to acetic acid being (60-80): (10-20):(0.15-0.18) as an extractant, adding the extractant and purple sweet potato powder in a container according to the weight ratio of extractant to purple sweet potato powder being 1: (5-20), and soaking the mixture at 30-40 DEG C for 1-2 h to obtain a crude extract; (3) auxiliary extraction through ultrasonic wave; (4) purification: adsorbing the mixture obtained in the step (3) through macroporous resin, eluting the mixture with ethanol after adsorption, collecting the eluent, performing concentration and drying on the eluent at 40-50 DEG C until that the solid content is greater than 10%, and then performing vacuum drying to obtain an anthocyanin finished product.

The invention discloses a method for extracting total DNA from an environmental sample with high efficiency for removing humus, comprising the following steps: (1) taking 0.3 g of sieved soil sample in a 2 mL centrifuge tube equipped with three 2.5 mm-3.0 mm sterilized glass beads, Add 1 mL of decomposing buffer, Vortex-Genie2 (Mobio Laboratories Inc) for 1 min, centrifuge at 12000 r / min for 2 min, discard the supernatant; (2) add 1 mL of 0.5 mol / L calcium chloride solution, Vortex-Genie2 (Mobio Laboratories Inc) ) Vortex for 2 min, centrifuge at 12,000 r / min for 1 min, and discard the supernatant; (3) Add 800 μL of DNA extraction buffer, and Vortex-Genie2 (Mobio Laboratories Inc) vortex for 5 s. Rapid and efficient extraction of total soil DNA, providing high-purity genomic DNA for soil metagenomics research.

The invention relates to an extraction method of inonotus obliquus polysaccharide. A high-voltage pulse electric field-assisted composite enzyme extraction method is mainly adopted for extracting inonotus obliquus powder multiple times, and the influence of the high-temperature environment of a traditional extraction method on the biological activity of the inonotus obliquus polysaccharide is overcome, and the extraction rate of the inonotus obliquus polysaccharide is improved.

The invention discloses a cordycepin extractive and application thereof in preparing hypoglycemic drugs or health-care foods. The cordycepin extractive is prepared by loading a liquid cordyceps culture solution on macroporous resin D-101 and adopting alcohol with the volume percent of 55% as an eluant. A preparation method of the cordycepin extractive comprises the steps of selecting the macroporous resin D-101 for absorption processing and optimally selecting parameters in the absorption processing process. Compared with the prior art, the preparation method not only has easy operation and technological condition control, but also has high cordycepin extraction purity. The cordycepin extractive is derived from the liquid cordyceps culture solution without poisoning human bodies by adding no harmful components in the purifying process and has good solvability and hypoglycemic effect.

The present invention belongs to the field of medical technology, and relates to a series of new iridoid compounds Loniceranan A, Loniceranan B, and Loniceranan C extracted and separated from dried buds of caprifoliaceae plants lonicera japonica, wherein the compounds comprise the same secoiridoid glucoside nucleus. The present invention also relates to the new compounds, hypoglycemic physiological activity of drugs prepared by using the compounds, and medical applications of the compounds . The compounds may be combined with a pharmaceutically acceptable carrier to form a clinically acceptable dosage form to be used for treatment of various diabetes. The preparation method of the present invention is simple and good in reproducibility, and the compounds are high in purity. The compounds obtained have good hypoglycemic activity.

The invention discloses a method for selectively removing Fe<2+> and / or Fe<3+> from industrial waste water through an electric adsorption technology. The industrial waste water contains Fe<2+> and / or Fe<3+> and other metal ions except the Fe<2+> and the Fe<3+>. The method uses nitrogen-sodium doped microporous carbon materials as electric adsorption electrode materials and comprises the following steps: (a) an adsorption process, wherein a voltage is applied to electrodes, so that the electric adsorption electrode materials adsorb various metal ions in the industrial waste water; (b) a desorption process, wherein the voltage applied to the electrodes is removed or reversely connected, so that the Fe<2+> and the Fe<3+> which are adsorbed on the electric adsorption electrode materials are not desorbed, and other metal ions are desorbed. The method disclosed by the invention has the advantages of being simple in operation, low in cost, low in energy consumption, free of secondary pollution and the like.

The invention relates to an industrialization production technology for extracting ginkgolic acid from Ginkgo testa. The technology comprises the following steps: leaching Ginkgo testa; separating, concentrating and fractionating leaching extracts; adjusting a pH value of the concentrated liquid; isolating a crude extract of ginkgolic acid; crystallizing, recrystallizing, and decolorizing by alkali liquor and acid precipitation; and further purifying the ginkgolic acid. The invention employs acid solution to regulate the pH value of the solution containing ginkgolic acid, and precipitates the ginkgolic acid by a common ion effect. The technology has the advantages of simple process, convenient operation, low cost, high extraction rate and high extraction purity, can rapidly extract ginkgolic acid in large amount from Ginkgo testa, and is suitable for requirements of industrial production.

The invention relates to a peptide containing 5-20 amino acids, in particular a glossy ganoderma polysaccharide protein G1-PP which is prepared by using glossy ganoderma as raw material precursor for extraction, condensing the extraction liquid, carrying out ultrafiltration or dialysis to keep the polysaccharide protein with high molecular weight. The invention also provides a process for industrial production of glossy ganoderma polysaccharide protein and the use of glossy ganoderma polysaccharide protein G1-PP in preparing medicament with antineoplastic action.

The invention belongs to the field of natural organic chemistry, relating to a method for enriching and purifying procyanidin in pine bark. The method is characterized in that an intermittent distillation and macropore adsorbent resin coupling technology is used to separate and purify the procyanidin, and the technology is good for absorption selectivity of the procyanidin; and by using the intermittent distillation and macropore adsorbent resin coupling technology, absorption is quick and deabsorption is also quick, absorption capacity is large, extraction is convenient and quick, raw material source is rich, production cost is low, separation effect is obvious, extraction purity is high, above 50% of semi-finished product procyanidin and above 95% of final product procyanidin can be achieved, and the defects of low conventional extraction rate and low extraction purity are overcome. In the method, the macropore adsorbent resin which has the advantages of stable physicochemical property, large surface area, fast exchange speed, high mechanical strength, strong pollution resistance capability and good heat stability is selected, the procyanidin is selectively absorbed from solution through physical absorption, absorption is quick and deabsorption is also quick, and absorption capacity is large.

The present invention discloses an integrated reaction separation method for preparing chitosan oligosaccharide and its equipment. After the chitosan is degraded by electrochemical reaction, said invention adopts analog moving bed to implement integrated separation and extraction of chitosan oligosaccharide. Besides, said invention also provides the electrochemical degradation reaction condition and concrete steps of integrated separation and extraction of said analog moving bed.

The invention discloses a kit for extracting genome DNA of an oral sample based on a magnetic bead method and an application method of the kit. The kit comprises a preservation liquid, a protease K solution, a lysate, a magnetic bead dispersion liquid, a cleaning liquid I, a cleaning liquid II and an eluent. The kit is simple and convenient to operate, and the work efficiency of extracting saliva or swab genome DNA can be greatly improved; and saliva does not need to be pretreated, and a saliva or swab sample can be split by adopting the lysate directly, and the magnetic bead dispersion liquid is added to be combined with the genome DNA, so that the extraction yield of nucleic acid is high and the kit is suitable for a high-throughput experiment.

The invention discloses an ultrasonic-microwave assisted enzymatic extraction method of green tea polyphenol. The method comprises the following steps: grinding weighed green tea leaves to obtain powder of which the particle size is 45 to 80 meshes, preparing the green tea powder into suspension liquid with water, at room temperature, using an ultrasonic-microwave extraction instrument of which the ultrasonic power is 45 to 60W, and the microwave power is 70 to 90W, after processing for 10 to 20 min, adding 1% to 3% of cellulase, carrying out ultrasound assisted enzymatic hydrolysis for 20 to 30 min, enzyme deactivation for 10 to 20 min under the conditions of a 100 DEG C-water bath, and centrifugation for 20 to 30 min at 5000 to 10000 r / min, performing pumping filtration after cooling, collecting filtrates, carrying out vacuum concentration to obtain a concentrated solution, then adding ethyl acetate to the concentrated solution to be extracted, and carrying out vacuum concentration, freeze concentration and freeze drying on organic phases, thereby being capable of obtaining the green tea polyphenol. The invention further provides shower gel containing the green tea polyphenol and a preparation method of the shower gel. The extraction method of the green tea polyphenol provided by the invention is simple to operate and high in extraction efficiency, furthermore, the green tea polyphenol extracted by the invention is applied to the shower gel, so that free radicals on the skin surface can be removed to play a good skin protective effect.

The invention discloses a method for extracting polyphenol from mango skins, and belongs to the field of plant extraction. The method comprises the following steps: taking the mango skins, washing the mango skins, drying the washed mango skins at 30-40DEG C, crushing the dried mango skins, and sieving the crushed mango skins by a 60 mesh sieve; carrying out ultrasonic extraction with an ethanol solution with the volume fraction of 30-50% as a solvent, carrying out pumping filtration, and drying the obtained extract liquid to obtain crude mango skin polyphenol extract; adding water to the crude polyphenol extract to prepare a solution with the concentration of 9-12mg / ml, and allowing the solution to go through macro-porous resin at a speed of 3.6-6.5BV / h; cleaning the macro-porous resin with water with the amount being greater than 5BV after the crude extract solution goes through the macro-porous resin; eluting with ethanol with the amount of 3-6BV and the concentration of 70-75% (v / v); and collecting the obtained eluate, and drying the collected eluate to obtain mango skin polyphenols. The method is a new method for utilizing the mango skins, changes wastes into valuables, and has the advantages of simple operation and high extraction rate.

The invention provides an extraction and purification method and application of a fermented astragalus polysaccharide. The extraction and purification method of the fermented astragalus polysaccharide comprises the following steps of (1) carrying out warm immersion extraction on fermented astragalus, concentrating an extracting solution by using a membrane intercepting ultrafilter, removing protein through a Sevag method, and carrying out dialysis, alcohol precipitation and washing to obtain a crude fermented astragalus polysaccharide; (2) purifying the fermented astragalus polysaccharide by adopting DEAE Sepharose FastFlow, eluting by using ultrapure water, concentrating and freeze-drying an eluant to obtain a neutral polysaccharide DF-1, eluting by using gradient NaCl, and concentrating, dialyzing, concentrating and freeze-drying the eluant to obtain a weakly acidic polysaccharide; (3) purifying the neutral polysaccharide DF-1 obtained from the step (2) by adopting a Sephacryl S-400 High Reslution preparation chromatographic column, eluting by using ultrapure water, concentrating and freeze-drying the eluant to obtain neutral polysaccharides SF-1 and SF-2. Compared with a crude drug APS, the FAPS (Fermented Astragalus Polysaccharide) extracted by applying the extraction and purification method provided by the invention is outstandingly increased in yield and content.

The invention provides an angelica dahurica coumarin extract, wherein the total content of coumarin is not lower than 44.672mg / g, and the content of imperatorin is not lower than 68.623mg / g. The invention further provides an extraction method and a quality control method of the extract. The angelica dahurica coumarin extract has high extraction purity, high content of coumatin, stable quality andfunction of 5-hydroxytryptamine regulation, is capable of treating diseases caused by 5-hydroxytryptamine besides migraine, has distinguished efficacy and strong pertinency of indications, and provides a new choice for clinical treatment.

The invention belongs to the field of natural organic chemistry, relating to a method for enriching and purifying schizandrol A in schisandra by utilizing macroporous adsorbent resin. The invention is characterized in that the macroporous adsorption technology, which is used for separating and purfying the schizandrol A, has good adsorption selectivity on the schizandrol A, rapid adsorption, rapid resolution and large adsorption capacity; and the extraction is convenient and fast, the source of raw materials is abundant, the production cost is low, the separating effect is obvious, the extraction purity is high, and the semi-finished product of the schizandrol A of which the content is more than 35% and the final product of the schizandrol A of which the content is more than 98% can be obtained, so that the defects that relatively low extraction rate and low extraction purity of routine extraction are overcomed. The macroporous absorbent resin selected by the invention has stable physicochemical property, larger surface area, fast exchange speed, high mechanical strength, strong anti-pollution ability and good thermostability, thereby being capable of selectively adsorbing the schizandrol A from solution by physical adsorption with the characteristics of fast adsorption, fast resolution and larger adsorption capacity.

The invention discloses a method for using subcritical water to extract grifolan. The method comprise the steps of stoving, smashing, subcritical extraction, rough filtration, heat preservation, centrifugation, concentration, purification, decolorization, precipitation, drying, re-washing, drying, secondary extraction and the like. The method specifically comprises the steps of taking advantages of good permeability and solubility of the subcritical water to make cell wall polysaccharide be in full contact with the subcritical water so that the cell wall polysaccharide can be fully dissolved out, conducting concentration on the cell wall polysaccharide through a water bath method, conducting purification on the grifolan by combining an Sevag method, and at the same time recycling a first centrifugation precipitation which can be used for conducting the secondary extraction on the grifolan. In this way, not only extraction content of the grifolan is increased, waste in raw material is also sharply reduced. Compared with existing other extraction methods, both the equipment cost and the operation cost according to the method for using the subcritical water to extract the grifolan are relatively lower, and extraction efficiency and purity of the grifolan are obviously improved.

The invention discloses first-stage rutin extraction equipment. A screw type thrust is connected with a backflow ultrasonic extraction pipe, the backflow ultrasonic extraction pipe is connected with a slag discharge mechanism and a revolving barrel type filter, the revolving barrel type filter is connected with an extract liquid storage tank, the extract liquid storage tank is connected with a revolving disc type filter, and the revolving disc type filter is connected with a gelatin and pigment separating mechanism. Compared with the prior art, the first-stage rutin extraction equipment can realize continuous production, is high in automation degree, and can increase rutin extraction purity and extraction rate.

The invention belongs to the field of natural organic chemistry and relates to a method for enriching and purifying common phellinus fungus general flavone in common phellinus fungus by using a batch distillation-macroporous absorption resin coupling method. The method is characterized in that the common phellinus fungus general flavone is separated and purified by applying a macropore adsorption technology, the adsorption selectivity to the common phellinus fungus general flavone is better, the separation effect is remarkable, the extraction purity is high, the semi-finished product with more than 35 percent of the common phellinus fungus general flavone and the final product with above 90 percent of the common phellinus fungus general flavone can be obtained, and the defects of relatively lower conventional extraction rate and low extraction purity are overcome. Macroporous absorption resin with stable physicochemical property, large surface area, higher switching speed, high mechanical strength, strong contamination resistance and good thermal stability is selected, and the common phellinus fungus general flavone is selectively absorbed from a solution through physical adsorption, thus the method has the advantages of high adsorption, high deabsorption speed and larger adsorption capacity.