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Cordycepin extractive and application thereof in preparing hypoglycemic drugs or health-care foods

A health food and extract technology, applied in the field of biopharmaceuticals, can solve the problems of complex equipment, low extraction rate, and low yield, and achieve the effects of easy control of process conditions, hypoglycemic effect, and simple operation

Inactive Publication Date: 2010-06-02
江西美俪佳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common extraction and purification methods of cordycepin include ion resin adsorption method, activated carbon adsorption method and supercritical extraction technology. However, the extraction and purification effects of these methods are not ideal. Low, the adsorption selectivity of the activated carbon adsorption method is poor, the yield is low, the equipment required for the supercritical extraction method is complicated, and the cost required is relatively high
[0004] At present, although some studies have shown that Cordyceps militaris has the effect of lowering blood sugar and blood lipids, this effect is not conferred by cordycepin, and there is no relevant report on the effect of cordycepin on hypoglycemic effect.

Method used

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  • Cordycepin extractive and application thereof in preparing hypoglycemic drugs or health-care foods
  • Cordycepin extractive and application thereof in preparing hypoglycemic drugs or health-care foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation and parameter optimization of embodiment 1 cordycepin extract

[0021] The cordycepin extract of the present embodiment, its preparation method comprises the steps:

[0022] Firstly, the liquid culture solution of Cordyceps militaris obtained by conventional method was selected as the raw material, and the liquid culture solution of Cordyceps militaris was concentrated into 1 / 5 of the original volume through a rotary evaporator, and the concentration temperature was 60°C; the polysaccharide was precipitated with 4 times the volume of absolute ethanol for 24 hours; A low-speed large-capacity centrifuge was used to separate the precipitate, and the supernatant was collected as a column sample, in which the concentration of cordycepin was 16.09 μg / mL.

[0023] Different macroporous adsorption resins were selected: S-8, NKA-9, D-101 and AB-8 for adsorption and desorption. It was found that the static adsorption capacity of D-101 macroporous resin was the highes...

Embodiment 2

[0032] (1) Kunming SPF mice 20±2g, after three days of adaptive feeding, fasting without food and water for 14 hours, each mouse was intraperitoneally injected with 1% alloxan at a dose of 200 mg / kg once. After 72 hours, the tail was taken for venous blood to measure blood sugar, and fasted for 8 hours before taking blood. The animals with blood glucose concentration greater than 11.1mmol / l were selected as hyperglycemia model animals.

[0033] (2) The hyperglycemia model mice were divided into a hyperglycemia model control group, a positive drug control group and a high-dose cordycepin extract treatment group, with 12 mice in each group. Twelve normal mice were taken as blank control group. The cordycepin extract high-dose treatment group was injected intraperitoneally with 72 mg / kg d of the cordycepin extract prepared in Example 1, the positive drug control group was intraperitoneally injected with phenformin hydrochloride 70 g / kg d, the hyperglycemia model control group an...

Embodiment 3

[0036] (1) Kunming SPF mice 20 ± 2 g, after three days of adaptive feeding, fasting without food and water for 14 hours, each mouse was intraperitoneally injected with 1% alloxan at a dose of 200 mg / kg once. After 72 hours, the tail was taken for venous blood to measure blood sugar, and fasted for 8 hours before taking blood. The animals with blood glucose concentration greater than 11.1mmol / l were selected as hyperglycemia model animals.

[0037] (2) The hyperglycemia model mice were divided into a hyperglycemia model control group, a positive drug control group and a middle-dose cordycepin extract treatment group, with 12 mice in each group. Twelve normal mice were taken as blank control group. Cordycepin extract medium dose treatment group was injected intraperitoneally with 24 mg / kg d of cordycepin extract prepared in Example 1, the positive drug control group was intraperitoneally injected with phenformin hydrochloride 70 g / kg d, hyperglycemia model control group and bla...

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Abstract

The invention discloses a cordycepin extractive and application thereof in preparing hypoglycemic drugs or health-care foods. The cordycepin extractive is prepared by loading a liquid cordyceps culture solution on macroporous resin D-101 and adopting alcohol with the volume percent of 55% as an eluant. A preparation method of the cordycepin extractive comprises the steps of selecting the macroporous resin D-101 for absorption processing and optimally selecting parameters in the absorption processing process. Compared with the prior art, the preparation method not only has easy operation and technological condition control, but also has high cordycepin extraction purity. The cordycepin extractive is derived from the liquid cordyceps culture solution without poisoning human bodies by adding no harmful components in the purifying process and has good solvability and hypoglycemic effect.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to fungal extracts with medicinal value, in particular to a cordycepin extract and its application in the preparation of hypoglycemic drugs or health food. Background technique [0002] Cordycepin (3'-deoxyadenosine) is a crystal purified from Cordyceps militaris. It is a new type of nucleoside antibiotic with antibacterial, antiviral, antitumor and immunomodulatory effects, which has caused global scientific and technological development. The country attaches great importance to it. It has inhibitory effect on both Bacillus subtilis and Candida strains; it also has inhibitory effect on HIV-I reverse transcriptase; especially it has strong inhibitory effect on various malignant tumors. Therefore, it is an inevitable trend to develop cordycepin as a new drug with a wide range of uses. [0003] Cordycepin mainly has two sources: chemical synthesis and extraction and purif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61K31/7076A61P3/10A23L1/29A23L33/00
Inventor 张松杜梅
Owner 江西美俪佳生物科技有限公司
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