142results about How to "Good hypoglycemic activity" patented technology

The present invention belongs to the field of medical technology, and relates to a series of new iridoid compounds Loniceranan A, Loniceranan B, and Loniceranan C extracted and separated from dried buds of caprifoliaceae plants lonicera japonica, wherein the compounds comprise the same secoiridoid glucoside nucleus. The present invention also relates to the new compounds, hypoglycemic physiological activity of drugs prepared by using the compounds, and medical applications of the compounds . The compounds may be combined with a pharmaceutically acceptable carrier to form a clinically acceptable dosage form to be used for treatment of various diabetes. The preparation method of the present invention is simple and good in reproducibility, and the compounds are high in purity. The compounds obtained have good hypoglycemic activity.

The invention discloses a composition of probiotic-fermented corn stigma as well as a preparation method of the composition. The composition is prepared by the following steps: drying and crushing the corn stigma, soaking the corn stigma, conducting probiotic fermentation, and adding a sweetening agent. The corn stigma, through the probiotic fermentation, is relatively high in content of corn stigma total saponins and relatively low in molecular weight of corn stigma polysaccharides; and the fermented corn stigma can take such effects on lowering blood glucose, reducing blood fat, relieving swelling and inducing diuresis, clearing away heat and toxic materials, calming liver and benefiting gallbladders, improving constipation and the like. The composition of the probiotic-fermented corn stigma disclosed by the invention has easily acceptable taste and the composition is non-irritant to gastrointestinal tracts; and meanwhile, the composition, which contains probiotics and metabolites thereof, is capable of conditioning the environment of the gastrointestinal tracts and enhancing drug absorption.

The invention belongs to the technical field of active site extraction and purification of traditional Chinese medicines, and relates to a costustoot extractive with a blood sugar reduction activity, which can be prepared by adopting a method comprising the following steps of: reflowing and extracting costustoot by using 70%-85% by volume fraction of alcohol, merging extracting solutions, and concentrating to an extract at a reduced pressure; and then adding water for dispersion, continuously extracting by using petroleum ether and ethyl acetate or n-butyl alcohol, and carrying out reduced-pressure concentration to remove the ethyl acetate or n-butyl alcohol extractive of an organic solvent to obtain the extractive with the blood sugar reduction activity. In the method, two-time extraction is carried out on the alcohol extractive of costustoot by using the optimized organic extracting solvent so that a blood sugar reduction active site is purified, and components irrelevant to the blood sugar reduction activity are reduced or removed so that the purified product has stronger blood sugar reduction activity, exacter curative effect, higher safety, reduction of dosages required for an equivalent activity level and convenience for administration, can be used for preparing medicaments for preventing and curing diabetes mellitus, and has a favorable development and application prospect in the prevention and curing field of the diabetes mellitus.

The present invention relates to the field of biotechnology and particularly relates to a method for preparing a soybean Aglycin family peptide by using acetic acid. The method for preparing the soybean Aglycin family peptide by using acetic acid comprises the following steps: subjecting raw materials to cleaning, soaking, germination, grinding and extraction, separating the slag from the slurry through a ceramic membrane and an organic membrane, and subjecting obtained materials to freeze-drying. According to the technical scheme of the invention, the acetic acid is extracted from soybeans, and the ceramic membrane and the organic membrane are combined to use, wherein the pore size of the ceramic membrane is 0.1 [mu]m and the pore size of the organic membrane is 1500 D to 6000 D. In this way, small protein and polypeptide compounds, with the molecular weight thereof between 1500 D to 6000 D, are obtained. The method is simple in operation, thoroughly in extraction effect, easy in filtration, short in production cycle, greatly improved in yield and greatly reduced in production cost. Therefore, the industrial production is easily realized. A produced Aglycin product is in the form of light yellow powders, and is good in taste and high in purity. The Aglycin product can be used as functional food for the adjuvant therapy of type 2 diabetes for preventing diabetes.

The invention provides a drug composite for curing diabetes and a preparation method and an application thereof, and belongs to the field of medicine. The drug composite for curing diabetes comprises at least one of Inoscavin C, 7,3'-dihydroxyl-5'-methoxyl isoflavone, 7,8-dihydroxy coumarin and 3,4-dihydroxy benzylidene acetone, and medically acceptable carriers or auxiliary materials. The application of the drug composite is wide and comprises that the drug composite is applied to preparing an agonist for promoting glucose uptake, preparing an agonist for using adenosine phosphate to activate protein kinase, preparing an agonist of glucose transport protein 4, and preparing a drug for curing or preventing diseases caused by metabolic disturbance or preparing health care products, a new treatment means and thought are provided for treatment of diseases caused by metabolic disturbance, and meanwhile new medical value of phellinus igniarius is further developed.

The invention belongs to the technical field of health care food and discloses sugarcane polyphenols extract and a preparation method and application thereof.The preparation method includes the steps that sugarcane or bagasse is taken, crushed and then sieved, coarse powder is obtained, then the coarse powder is extracted with an ethanol solution, filtering is conducted to remove filter residues, and filtrate is subjected to decompression and concentrated to extractum; water is added to the obtained extractum for dispersion, petroleum ether is adopted for extraction, a petroleum ether phase and a water phase are obtained, the water phase is extracted with ethyl acetate, an ethyl acetate phase is collected, and the ethyl acetate phase is subjected to decompression and concentrated to extractum; then chromatographic separation is conducted with a polyamide column, ethyl alcohol is subjected to gradient elute, eluate with the alcohol volume concentration being 30% is collected, the eluate is concentrated and dried, and the sugarcane polyphenols extract is obtained.The sugarcane polyphenols extract is made of staple crops, namely sugarcane, multistage extraction and column chromatography gradient elution separation and purification are conducted, and a product has higher hypoglycemic activity and can be used for food additives and functional food.

The invention discloses a method for preparing bitter gourd freeze-dried powder capable of reducing blood glucose. The method comprises the following steps that: A, bitter gourd fruits are washed clean; B, the tissue of bitter gourd fruit is fully broken at a temperature below 30 DEG C so as to fully release intracellular matter and obtain bitter gourd fruit paste with pulp; C, a complex enzyme preparation E1 which has pectinase and cellulose enzyme complexes as components and the bitter gourd fruit paste are mixed in a stir-type enzyme reactor, and react for 1.5 to 2 hours at a temperature of between 45 and 55 DEG C; the pH value is controlled between 4 and 5; a complex enzyme preparation E2 comprising bacterial protease and glucoside hydrolytic complex enzyme is added and reacts with the complex enzyme preparation E1 and the bitter gourd fruit paste for 0.5 to 1 hour at a temperature of between 40 and 60 DEG C; the pH value is controlled to be between 5.5 and 7; and then the temperature is adjusted to be between 75 and 85 DEG C and kept for 15 and 20 minutes so as to inactivate the complex enzyme preparations; and D, the fruit paste after enzyme treatment is concentrated for 3 to 4 hours in a vacuum dryer at the vacuum degree between 1 and 5 kPa and at a temperature lower than or equal to 45 DEG C so as to remove most of moisture, and then is freeze-dried in a freeze dryer under the pressure lower than 10 Pa and at a temperature lower than 20 DEG C so as to obtain the bitter gourd freeze-dried powder. The bitter gourd freeze-dried powder obtained by the method has the advantage of good effect of reducing blood glucose.

The invention discloses a lactobacillus rhamnosus exopolysaccharide, and a preparation method and application thereof, and belongs to the technical field of microorganisms. According to the invention, a lactobacillus rhamnosus strain separated from Xiangshuangpao yoghurt is used as an original strain to produce exopolysaccharide, the exopolysaccharide is a uniform component, and the molecular weight of the exopolysaccharide is 88650 Da; and the lactobacillus rhamnosus exopolysaccharide comprises mannose, glucuronic acid, glucose, xylose, galactose and arabinose. The exopolysaccharide has a good in-vitro hypoglycemic effect, has good in-vitro alpha-glucosidase inhibition capacity through detection, and is simple in process, wide in market prospect, stable in thermodynamic property and suitable for large-scale production. Therefore, the polysaccharide is a potential active ingredient for preventing and treating diabetes mellitus. Besides, as a fermentation product of probiotics, compared with common chemical medicines in the field of blood sugar reduction, the fermentation product has the advantages of simple and easily available active ingredients, low price, no side effect and the like, and has great application value.

The present invention provides a precursor polypeptide or a pharmaceutically acceptable salt of a glucagon-like peptide-1 analogue, wherein the precursor polypeptide has an amino acid sequence represented by the following general formula I HX1X2GTFTSDVSSYLEEX3AAX4EFIX5WLVKX6X7X8X9, and X1, X2, X3, X4, X5, X6, X7, X8 and X9 represent arbitrary amino acids. The present invention further provides a glucagon-like peptide-1 analog or a pharmaceutically acceptable salt thereof. According to the present invention, the glucagon-like peptide-1 analog is obtained by conjugating a polypeptide optimized based on an endogenous GLP-1 (7-36 / 37) sequence and polyethylene glycol having a specific structure, has strong blood glucose lowering activity so as to reduce the dosage, and has significantly-prolonged half-life in vivo so as to be expected to improve clinical compliance; and the precursor polypeptide and the endogenous GLP-1 (7-36 / 37) are highly homologous in the sequence so as to reduce the immunogenicity, such that the precursor polypeptide has good potential in the drug development application.

The invention discloses rubus hirsutus anthocyanin and application thereof. The rubus hirsutus anthocyanin mainly comprises cyanidin-3-O-glucoside, pelargonine 3-O-glucoside and pelargonine 3-O-rutinoside, wherein the pelargonine 3-O-glucoside is main anthocyanin. An animal experiment proves that the rubus hirsutus anthocyanin can significantly reduce fasting blood glucose of db / db diabetic mice and shows excellent hypoglycemic activity in vivo. An in-vitro experiment proves that the rubus hirsutus anthocyanin can play a hypoglycemic role by inhibiting the alpha-glucosidase activity and promoting the glucose consumption of cells. Therefore, the rubus hirsutus anthocyanin can be taken as hypoglycemic functional food or a hypoglycemic drug for the prevention and the treatment of glycometabolism disorder-related diseases. The invention provides a new idea for comprehensive development and utilization of rubus hirsutus resources in China.

The invention discloses a preparation method of balsam pear / green tea sugar-reducing buccal tablets. The balsam pear green tea sugar-reducing buccal tablets are characterized by the following raw materials in parts by weight: 0.2-0.4g of balsam pear educt, 0.1-03g of instant green tea powder, 1.0-1.3g of corn starch, 0.5-0.8g of beta-annular dextrin, and 3.0-4.0 g of xylitol, the right amount ethanol is added to wet for granulation after the raw materials are fully mixed to be uniform, the granules are dried in a baking oven at 50-60 DEG C, and buccal tablets of a certain standard are prepared through pressing. The hardness of the buccal tablets is moderate after being pressed by adopting the technical formulation, The buccal tablets have no an adhesion feel, the sweetness is proper, and the buccal tablets have specific sweetness and bitterness and tea incense relish of balsam pears and green tea. The preparative balsam pear green tea sugar-reducing buccal tablets provided by the invention have an subsidiary sugar reducing pharmacological functions and has a certain food therapy nutritive value, the mouthfeel is good, the carry and consumption are convenient, and the balsam pear green tea sugar-reducing buccal tablets have better market application and popularization value.

The invention discloses a coptis alkaloid and ferulic acid compound with glucose-lowering effect, which comprises the following components in percentage by weight: 0.1 to 99.9 percent of coptis alkaloid and 0.1 to 99.9 percent of ferulic acid, wherein the coptis alkaloid contains berberine or coptisine or palmatine or jatrorrhizine or the sum of four coptis alkaloids, or is total coptis alkaloids extracted from coptis. The compound has the advantages of better glucose-lowering effect compared with that of the coptis alkaloid or ferulic acid, and obvious glucose-lowering effect according to administration in a dosage of 0.5 to 5 grams every person per day.

The invention provides longan pericarp extract as well as an extracting method, a composition and application thereof. The extracting method comprises the following steps: extracting longan pericarp through an alcohol aqueous solution to obtain an extracting solution; concentrating the extracting solution to obtain crude extract; and performing column chromatography on the crude extract to obtain the longan pericarp extract, wherein the extracting is preferably performed by 1-3 times; based on the dried longan pericarp, weight of the alcohol aqueous solution used for every extracting is preferably 10-30 times the weight of the raw materials; the volume concentration of the alcohol aqueous solution is preferably 20-95%. The longan pericarp extract is firstly found to have relatively good blood sugar reducing activity and have advantages that the chemical components are remarkably different from those of kernel, the active substances are relatively rich, the raw materials are easily available, and the like.

The invention discloses phyllostachys heterocycla bamboo shoot shell arabinogalactan as well as preparation and an application thereof. The phyllostachys heterocycla bamboo shoot shell arabinogalactanis prepared from 99% or more by weight of polysaccharide; the polysaccharide is prepared from galactose, arabinose, xylose and galacturonic acid in a mole ratio being (6.80-8.50):(5.50-7.30):(1.10-1.90):(0.70-1.30). The preparation method of the phyllostachys heterocycla bamboo shoot shell arabinogalactan comprises the following steps: extracting phyllostachys heterocycla bamboo shoot shell crudepolysaccharide by water extraction and alcohol precipitation, removing protein with an enzyme-Sevage combined method, performing dialysis, anion exchange chromatography and gel filtration chromatography for purification, and separating and purifying components by vacuum freeze drying to obtain the phyllostachys heterocycla bamboo shoot shell arabinogalactan. According to component analysis, structural identification and immune function research on the purified polysaccharide, the phyllostachys heterocycla bamboo shoot shell arabinogalactan has obvious hypoglycemic activity, can be used as a hypoglycemic functional product, can also be used for preparing a hypoglycemic functional product, and can be widely used in the fields of food, health care products, animal feed, medicine and the like.

The invention relates to the technical field of natural product deep processing, in particular to a novel extraction method of momordica saponins. The novel extraction method comprises the following steps that 1, dry bitter gourd powder is taken to be dissolved into ethyl alcohol, oscillating and shaking are conducted to enable the mixture to be uniform, the mixture is put into a 60-DEG C water bath kettle to be extracted for 1 hour and then centrifuged to obtain a momordica saponins alcohol extracting solution, the momordica saponins alcohol extracting solution is subjected to vacuum concentration in a rotary evaporator until a concentrated solution is free of alcohol smell, and then a momordica saponins extracting solution A is prepared; 2, extraction is conducted by adopting an isopropyl alcohol-(NH4)2SO4 aqueous two-phase system, after partition equilibrium is achieved, phase separation is conducted, an upper phase is take, and a momordica saponins extracting solution B is prepared; 3, the crude momordica saponins extracting solution B is further purified through AB-8 type macroporous adsorption resin, and a fine momordica saponins extract C is prepared; 4, freeze drying is conducted to prepare the finished momordica saponins. Compared with a traditional method, the momordica saponins prepared through ethanol extraction, aqueous two-phase extraction and macroporous resin separation has the advantages that the yield of the momordica saponins is increased, and the blood glucose reducing activity of the momordica saponins is also obviously improved.

The invention discloses a siraitia grosvenorii endophyte bacterial strain capable of producing extracellular polysaccharides, a method thereof for producing the extracellular polysaccharides and application of the extracellular polysaccharides, and belongs to the technical field of microorganisms. The name of the bacterial strain is bacillus sp. ND-6, the preservation unit is the general microbialcenter of China microbial strain preservation management committee, the preservation number is CGMCC No. 15227, and the preservation date is January 16, 2018. The invention further discloses a methodfor producing the extracellular polysaccharides by utilizing the siraitia grosvenorii endophyte bacterial strain capable of producing the extracellular polysaccharides and application. The siraitia grosvenorii endophyte bacterial strain can produce the extracellular polysaccharides, and the extracellular polysaccharides the effects of reducing blood glucose and preventing oxidation in vitro, a new idea is provided for the development of diabetes prevention and treatment drugs, and the siraitia grosvenorii endophyte bacterial strain is very promising bacterial strain.

The invention relates to a phellinus igniarius phenol extract with blood sugar reducing activity, a preparation method for the phellinus igniarius phenol extract and application of the phellinus igniarius phenol extract. According to the preparation method, an ethyl acetate extract with relatively high phenol purity (not lower than 70%) is obtained through ultrasonic ethanol aqueous solution assisted extracting and solvent branched extracting and mainly comprises substances such as osmudacetone, hispidin, davallialactone, 2,5-bis(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)cyclohexa-2,5-diene-1,4-dione, hypholomine B and inoscavin A. The phellinus igniarius phenol extract disclosed by the invention is high in phenol content, has a remarkable inhibiting action on alpha-glycosidase, has anobvious promoting action on glucose consumption of cells HepG2 and has good blood sugar reducing activity and potential diabetes preventing and treating effect. The preparation method is relatively simple in process procedure, free of a fine separating step, relatively high in preparation efficiency and relatively low in cost and is applicable to subsequent pilot trial and industrial production.

The invention relates to the field of medicinal use of anthocyanin and discloses an anthocyanin-contained composition and application thereof. If anthocyanin is extracted from grape skin, the composition is applied to antioxidation drugs, plays a role in clearing away DPPH free radicals, superoxide anion free radicals (O2<->.) and hydroxyl free radicals (.OH) and has a relatively high reducing property; if the anthocyanin is extracted from mulberries, the composition is applied to bacteriostatic drugs and plays a role in inhibiting staphylococcus aureus, Escherichia coli and bacillus subtilis; and the composition can also be applied to blood sugar reducing, blood fat reducing and atherosclerosis inhibiting and plays a role in inhibiting intestinal alpha-glucosidase, the blood sugar reducing activity of acarbose is enhanced obviously, the synthesis of fats is inhibited, the decomposition of the fats is stimulated, and the composition has blood sugar reducing and blood fat reducing actions and can be used for inhibiting expression of adhesion molecules and inhibiting formation of atherosclerosis.

The invention relates to a long-lasting glucagon-like peptide-1 (GLP-1) analogue for treating diabetes. The GLP-1 analogue is obtained by modifying the natural GLP-1 and has longer duration of pharmacologic action; proved by experiments, the GLP-1 analogue can achieve a better diabetes treatment effect.

The invention provides a jackfruit leaf polysaccharide as well as a preparation method and an application thereof. The jackfruit leaf polysaccharide is a polysaccharide with a molecular weight of less than 100kDa and has a blood sugar-reducing function. The polysaccharide is prepared by the method comprising the following steps: extracting jackfruit leaf crude powder by hot water, concentrating the extract and then carrying out column chromatography separation by a macroporous adsorption resin, concentrating a pure water eluate and then filtering by an ultrafiltration membrane with a molecular weight cut-off of 100kDa, and carrying out alcohol precipitation on the ultrafiltrate to obtain the jackfruit leaf polysaccharide. The preparation method for the jackfruit leaf polysaccharide has the characteristics of being simple in process, low in cost, and suitable for industrial production. The jackfruit leaf polysaccharide provided by the invention can be applied to medicines and foods with a blood sugar-reducing effect.

The invention discloses a composition of an Exendin-4 derivative and a preparation method and application thereof. The composition of the Exendin-4 derivative consists of the Exendin-4 derivative and mono-methyl polyethylene glycol activated by maleimide. The composition of the Exendin-4 derivative has high biostability, long half-life in vivo, and high bioavailability. The preparation method is easy and convenient to implement and raw materials are readily available.

The invention discloses an ocean health care capsule having efficacy of lowering blood sugar. The capsule is prepared by scientific compatibility of astaxanthin and raw materials of Chinese herbal medicine, preferably, the capsule is prepared from the following ingredients in parts by weight: 30-40 parts of astaxanthin, 15-20 parts of chitosan, 15-18 parts of medlar, 10-14 parts of corn stigma, 8-10 parts of rehmannia root, 8-10 parts of liquorice, 6-10 parts of semen cassiae, 4-8 parts of rhizoma atractylodis macrocephalae, 5-8 parts of medicated leaven, 4-6 parts of platycodon grandiflorus, 4-6 parts of rhizoma anemarrhenae, 4-6 parts of phyllanthus emblica, 3-5 parts of herb of common seepweed and 2-3 parts of stevia rebaudiana, and then the capsule is prepared according to the process of mixing, blending, sterilizing and filling. The product has the advantages that by the synergistic effect of the chitosan, the astaxanthin and the Chinese herbal medicine, the product has the efficacy of remarkably lowering blood sugar, tonifying Qi, supporting the healthy energy, resisting fatigue, prolonging life and the like, the product is safe, has no side effect, is convenient to eat and has a good market application prospect.

The invention discloses a novel schiff base vanadium oxide compound as well as a preparation method and application thereof. The novel schiff base vanadium oxide compound is named N,N'-bis(salicylidene)-4,5-dichloro-1,2-phenylenediamine shrinked-diamine oxovanadium and has a molecular formula of [C20H12Cl2N2O3V] and molecular weight FW equal to 450.16, and a chemical structural formula of the novel schiff base vanadium oxide compound is as shown in the specification. The novel schiff base vanadium oxide compound disclosed by the invention has excellent hypoglycemic activity and can be used for preparing a drug for treating diabetes.

The invention belongs to a liposome combined with a GLP-1 (Glucagon-Like Peptide-1) analogue and polyethylene glycol and a preparation method of the liposome. The liposome is prepared by combining a polyethylene glycol part on one part of sulfhydrylated liposome on the tail end of lipid through a disulfide bond and combining with the GLP-1 analogue through a disulfide bond. In the terms of 1 mol of sulfhydrylated lipid in the liposome, the combination quantity of PEG (Polyethylene Glycol) and the combination quantity of the GLP-1 analogue are respectively 15-50mol percent and 0.5-10mol percent. According to the liposome, the bioactivity of a GLP-1 polypeptide is kept, the stability of the GLP-1 analogue in blood is improved, and the half-life period of the GLP-1 analogue in vivo is prolonged.

The invention discloses an artemisia rupestris preparation used in medicaments for treating diabetes and a preparation method thereof. The preparation method comprises the following steps: crushing artemisia rupestris herbs; weighing the powder; adding ethyl ether of which the volume is 3 times of the volume of the powder; standing for 8 hours, and then, filtering; ultrasonically extracting precipitates with 80% ethanol at 35 DEG C for 30-40 minutes every time; filtering to obtain an ethanol extracting solution; precipitating with 95% ethanol, centrifuging, and taking a supernatant solution; extracting ethyl acetate; filtering to obtain an ethyl acetate part; carrying out polyamide column chromatography on the ethyl acetate part; eluting by sequentially adopting ethanol solutions with different concentrations of 20%, 40%, 60% and 80%; collecting ethanol eluate obtained by adopting the ethanol solutions with the concentrations of 60% and 80%; concentrating while depressurizing to obtain an extract; proportioning the obtained extract and a milk vetch extract according to the weight ratio of (5-10):1; and preparing a certain amount of pure water to obtain a finished preparation. The prepared artemisia rupestris preparation has the activity of reducing blood sugar, the using dose of the artemisia rupestris preparation is 67.5mg / kg, and the artemisia rupestris preparation has wide application fields in medicaments for treating diabetes.

The invention discloses marine functional compound-prepared wine with the blood sugar lowering effect. The marine functional compound-prepared wine is prepared by scientifically combining with astaxanthin and traditional Chinese medicine raw materials, preferably according to a formula comprising, 10-20 parts of chitosan, 10-20 parts of the astaxanthin, 15-20 parts of wolfberries, 10-15 parts of Chinese yams, 10-20 parts of schisandra chinensis, 5-10 parts of angelica sinensis, 10-15 parts of semen coisis, 5-8 parts of ligusticum wallichii, 3-5 parts of cistanche, 3-5 parts of roots of kudzu vine, 4-6 parts of dandelion, 2-3 parts of herba houttuyniae, 3-5 parts of liquorice and 3-5 parts of stevia rebaudiana, and the wine is prepared by the process of digestion, mixing, blending and filling and sterilizing. The marine functional compound-prepared wine has the advantages of remarkable efficacy of blood sugar level decreasing, qi tonifying, healthy energy strengthening and consolidating, fatigue resisting, life prolonging and the like is achieved through synergistic effect among the chitosan, the astaxanthin and Chinese herbs, and the wine is safe without side effects, convenient to drink and good in market application prospect.

Disclosed is a bis(2-ethyl-3hydroxy-4-pyranone) copper(II) complex and its use in treating diabetes, which relates to heterocyclic compound having oxygen atoms, especially a copper central ionic 2-ethyl-3-hydroxy-4-pyranone chelate and its use. The copper complex has a molecular formula of C14H14CuO6, and a formula weight of FW=341.5. The compound can be made into oral administration medicines against diabetes.