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Siraitia grosvenorii endophyte bacterial strain capable of producing extracellular polysaccharides, method thereof for producing extracellular polysaccharides and application of extracellular polysaccharides

A technology of extracellular polysaccharides and endophytes, applied in the field of microorganisms, can solve problems such as low content of antioxidant substances, destruction of animal and plant resources, loss of biodiversity and ecological environment, etc., and achieve strong antioxidant activity and strong hypoglycemic activity , good in vitro hypoglycemic activity

Active Publication Date: 2018-09-04
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of natural antioxidant substances in animals and plants is low, the extraction process is complicated, the resources are limited, and these animal and plant resources will be severely damaged, resulting in the loss of biodiversity and ecological environment.
It is a good way to obtain antioxidant drugs through microbial fermentation
However, there is no relevant report on the strains of polysaccharides with in vitro hypoglycemic activity and anti-oxidation produced by Bacillus genus obtained from the endophyte of Luo Han Guo.

Method used

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  • Siraitia grosvenorii endophyte bacterial strain capable of producing extracellular polysaccharides, method thereof for producing extracellular polysaccharides and application of extracellular polysaccharides
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  • Siraitia grosvenorii endophyte bacterial strain capable of producing extracellular polysaccharides, method thereof for producing extracellular polysaccharides and application of extracellular polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1: A strain of Luo Han Guo endophytic bacteria producing exopolysaccharide

[0085] A strain of Luo Han Guo endophytic bacteria producing exopolysaccharide, the name of the strain is Bacillus ND-6 (Bacillussp. The number is CGMCC No.15227, and the deposit date is January 16, 2018.

Embodiment 2

[0086] Example 2: Screening method for the above-mentioned exopolysaccharide-producing endophytic strains of Luo Han Guo

[0087] The screening method of the above-mentioned exopolysaccharide-producing endophytic bacteria strains of Luo Han Guo comprises the following steps:

[0088] Step 1: Isolate and purify the endophytic strains of Luo Han Guo from the roots, stems and fruits of Luo Han Guo

[0089] Disinfection of Luo Han Guo materials: On the ultra-clean workbench, the cleaned roots, stems and fruits of Luo Han Guo are subjected to surface disinfection treatment by the following methods.

[0090] Roots: soak in 75% (v / v) alcohol for 2 minutes, then soak in 3.0% NaClO solution for 5 minutes, then soak in 75% (v / v) alcohol for 30 seconds, and finally rinse with sterile water for 5 times. 5 minutes each time.

[0091] Stems: Soak in 75% (v / v) alcohol for 3 minutes, then soak in 3.0% NaClO solution for 8 minutes, then soak in 75% (v / v) alcohol for 30 seconds, and finally r...

Embodiment 3

[0181] Example 3: Identification of the above-mentioned exopolysaccharide-producing endophytic strains of Luo Han Guo

[0182] Such as figure 1 , figure 2 Shown, the Luo Han Guo endophytic bacteria strain that produces exopolysaccharide of the present invention has the following characteristics:

[0183] (1) Individual morphology: the bacterium is rod-shaped, Gram staining is positive, and there are spores, and the spores are located in the middle of the bacterium.

[0184] (2) Colony traits on the solid medium plate: the colony is round, wrinkled in the middle, smooth at the edge, and slightly yellow.

[0185] (3) The fermentation liquid in the liquid fermentation medium is turbid, with a bacterial film on the liquid surface and flocculent sediment at the bottom.

[0186] (4) The optimum temperature for growth is 30-50°C, pH 5.0-6.0, and aerobic.

[0187] The above solid medium is composed of glucose 10g, peptone 5g, yeast extract 5g, K 2 HPO 4 0.2g, MgSO 4 ·7H 2 O0...

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Abstract

The invention discloses a siraitia grosvenorii endophyte bacterial strain capable of producing extracellular polysaccharides, a method thereof for producing the extracellular polysaccharides and application of the extracellular polysaccharides, and belongs to the technical field of microorganisms. The name of the bacterial strain is bacillus sp. ND-6, the preservation unit is the general microbialcenter of China microbial strain preservation management committee, the preservation number is CGMCC No. 15227, and the preservation date is January 16, 2018. The invention further discloses a methodfor producing the extracellular polysaccharides by utilizing the siraitia grosvenorii endophyte bacterial strain capable of producing the extracellular polysaccharides and application. The siraitia grosvenorii endophyte bacterial strain can produce the extracellular polysaccharides, and the extracellular polysaccharides the effects of reducing blood glucose and preventing oxidation in vitro, a new idea is provided for the development of diabetes prevention and treatment drugs, and the siraitia grosvenorii endophyte bacterial strain is very promising bacterial strain.

Description

technical field [0001] The invention relates to a Luo Han Guo endophytic bacteria strain producing extracellular polysaccharides, a method for producing extracellular polysaccharides and an application of the extracellular polysaccharides, and belongs to the technical field of microorganisms. Background technique [0002] Luo Han Guo [Siraitia grosvenorii (Swingle) C. Jeffrey] is the fruit of the perennial rhizome vine Luo Han Guo of Cucurbitaceae Luo Han Guo. It is a unique plant in my country and grows in Guangxi, Guangdong, Jiangxi, Hunan and Guizhou. Yongfu County is the birthplace and main producing area of ​​Luo Han Guo. Luo Han Guo fruit is rich in a variety of essential amino acids, trace elements, and multivitamins. In addition, it contains active ingredients such as flavonoids, polysaccharides, sweet glycosides, and polyphenols. Modern pharmacological research has confirmed that Luo Han Guo has a variety of pharmacological effects such as antitussive and asthma, la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04A61K31/715A61P3/10A61P39/06C08B37/00C12R1/07
CPCA61K31/715A61P3/10A61P39/06C08B37/0003C12P19/04C12N1/205C12R2001/07
Inventor 赵丰丽张昌志范彩琴龙楚媚付强陈静
Owner GUANGXI NORMAL UNIV
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