317 results about "Galacturonic acid" patented technology

A nucleic acid formulation for use in gene delivery comprising a nucleic acid and an anionic polymer is disclosed. Examples of the anionic polymer includes anionic amino acid polymer or poly-amino acid (such as poly-L-glutamic acid, poly-D-glutamic acid, poly-L-aspartic acid, poly-D-aspartic acid), poly-acrylic acid, polynucleotides, poly galacturonic acid, and poly vinyl sulfate.

Processes for producing ethanol from citrus waste by reducing the concentration of limonene in citrus waste to allow fermentation are disclosed. In one embodiment a slurry of ground citrus waste 1 is partially hydrolyzed by heating using a jet cooker 2 and then injected into a flash tank 4 to remove limonene 5. The heated citrus waste is then cooled, hydrolyzed with enzymes and fermented to ethanol. An alternative method of limonene removal uses enzymatic hydrolysis followed by centrifugation 27 to separate sugar-containing liquid from residual citrus waste solids containing limonene. Sugars are fermented and ethanol is distilled from the fermented mixture / beer. The remaining solids and liquids may be processed further to yield other byproducts. More particularly, the solids may be dried and pressed for use in cattle feed and the liquids may be further fermented or processed to yield additional ethanol, acetate, galacturonic acid monomers and polymers, five carbon sugars and other products.

The invention relates to a colloid bismuth pectin compound and a quality control method of pharmaceutical compositions of the colloid bismuth pectin compound, and specifically provides a colloid bismuth pectin compound, new quality control indexes of pharmaceutical compositions of the colloid bismuth pectin compound and a detection method of the colloid bismuth pectin compound and the pharmaceutical compositions of the colloid bismuth. In the prior art, the quality of products having poor efficacy can not be well controlled because the efficacy-related indexes are not controlled strictly in the existing quality control methods of colloid bismuth pectin and pharmaceuticals thereof. Based on the existing quality control methods of colloid bismuth pectin and pharmaceuticals thereof, the following detection items and indexes are added: intrinsic viscosity, gel property, uniformity and galacturonic acid content. The invention can effectively ensure clinical efficacy of products, make the product quality standards more scientific, reasonable and controllable, and has great significance in quality control of the colloid bismuth pectin and the pharmaceutical preparation of colloid bismuth pectin.

The invention discloses a Ziyang selenium-rich green tea selenium-containing polysaccharide, which comprises the following components in percentage by mass: 1.5-8.5 ppm selenium and the balance of heterosaccharide. The heterosaccharide comprises the following components in percentage by mol: 3.3-5.3% of mannitose, 1-2% of ribose, 3.5-5% of rhamnose, 2-3.5% of glucuronic acid, 2.6-3.8% of galacturonic acid, 30-34.2% of glucose, 3.6-5.6% of xylose, 19.8-23.2% of galactose and 21.5-25.6% of arabinose. The invention also provides a preparation method and application of the Ziyang selenium-rich green tea selenium-containing polysaccharide. The preparation method is simple and convenient, has the advantage of low cost, and is suitable for popularization; and the selenium-containing polysaccharide has the action of enhancing immunity, has obvious inhibiting actions on growth of colon cancer cells, and has obvious protective actions on hepatic injury.

The invention relates to a method for extracting natural low-ester pectin from sunflower heads. The method comprises the following steps: washing; extracting; carrying out ultrafiltration desalination; carrying out ion exchange to remove residual salt; carrying out spray drying; carrying out reverse osmosis; and recovering calcium citrate, wherein a chelating agent used for extraction is sodium citrate, the concentration is 0.4%-1.0% (sodium citrate: water), the extraction solid-to-liquid ratio is 1:20-1:50 (sunflower head dry powder: water), the pH is regulated to 2-4, then a great part of salt is removed by using an ultrafiltration membrane with the interception molecular weight of 5000-10000 Da, and the residual salt is removed by adopting the ion exchange. The method disclosed by the invention can achieve the pectin extraction rate by 10%-15% (in terms of galacturonic acid on raw materials) and is capable of removing the salt by more than 90% through the ultrafiltration desalination; and as for the prepared low-ester pectin, the esterification degree is 25%-30%, the amidation degree is 3%-10%, the content of the galacturonic acid is 65%-80% (on the pectin), and the weight-average molecular weight is 200-300 kDa. According to the method, the sodium citrate as the chelating agent has very high safety and can realize calcium citrate recovery through the addition of calcium hydroxide after reverse osmosis.

The invention relates to a new application of low molecular citrus pectin, in particular to the application of low molecular citrus pectin for treating and preventing lead and heavy metal poisoning in prepared medicines, foods and health care products. The low molecular citrus pectin is a compound prepared from soluble dietary fiber and galacturonic acid, the polysaccharide derivative is used as a metal ion absorbent (chelating agent), has absorption action on heavy metal ions, can remove heavy ions, such as Zn2+, Cu2+, Cd2+, Ag+, Cr3+, Ni2+, Hg2+, Pb2+ and the like. The low molecular citrus pectin is different from common chemical chelating agents; proved by animal and clinic experiments, lead, mercury and arsenic are discharged out of human bodies during toxin discharge, and the key level of calcium, magnesium, zinc or other minerals can not be influenced. The natural chelating function of the low molecular citrus pectin is a most ideal and successful solution for heavy metal pollution in the human body at present. Thus, the low molecular citrus pectin has the function of preventing various diseases and especially has special functions of clearing away harmful cells and discharging toxins in the human body.

The invention discloses a method for improving yield of salvianolic acid B in savia miltiorrhiza suspension culture cells by inducing, specifically comprising the following steps: 1) cultivating the aseptic seedlings; 2) inducing the callus; 3) sub-cultivating the callus; 4) cultivating the callus by suspending; 5) inducing to produce the salvianolic acid B; and 6) harvesting the culture cells. The method solves the problem that the salvianolic acid B is not produced after the inducing factors, such as yeast extract, galacturonic acid, mycelia extract and the like are added. The content of the salvianolic acid B in the savia miltiorrhiza suspension culture cells is increased by about 5 times by using the method. The technology condition provided by the invention is suitable for industrial production of the salvianolic acid B on a large scale by a cell culture method after being expanded.

The invention discloses a method for treating canned citrus petal pectin wastewater. By employing the method for combining mechanical bar screen and coagulating sedimentation, aerated air floatation and microbial advanced treatment, a mature strain of fungus penicillium simplicissimum is added into a microbial reaction pool in the microbial advanced treatment process, the pectin in the wastewater is degraded into low molecular weight organic matter galacturonic acid and methyl galacturonase under the catalytic action of pectinase generated by the mature strain of penicillium simplicissimum, and the highest degradation efficiency of the penicillium simplicissimum on the pectin is 97.4 percent; meanwhile, the penicillium simplicissimum has a good effect on the degradation of the CODCr in the wastewater, and the highest degradation efficiency on the CODCr is 89.9 percent; moreover, according to the process flow, the wastewater is extremely low in organic content and viscosity, the GB8978-1996 secondary emission standard of the integrated wastewater discharge standard is achieved, and emission on standard is realized. The process has the advantages that the energy consumption is low, the process is easy to operate, secondary pollution is not caused, and the like.

The invention discloses a Chinese magnoliavine fruit polysaccharide extract and a preparation method and an application thereof. Counted by D-glucose, the content of polysaccharides is not lower than 45 percent of the total weight of the polysaccharide extract; a monosaccharide mainly comprises D-glucose, D-galactose and D-galacturonic acid; the mass ratio of the D-glucose to the D-galactose is (3-9):1; and the D-galacturonic acid accounts for 5-40 percent of the total weight of the polysaccharide extract. As proved by mouse ear heterogeneous driven dermal sensitivity test and rat peritoneal mast cell degranulation test, the Chinese magnoliavine fruit polysaccharide extract has remarkable anti-allergic action activity, and can be used for preparing anti-allergic action medicaments, functional foods and cosmetics.

Method for producing ethanol from solid citrus waste by reducing the concentration of limonene in citrus waste to allow fermentation. In one embodiment ground solid citrus waste is partially hydrolyzed and pasteurized by heating using a jet cooker and then injected into a flash tank to remove limonene. The heated citrus waste is then cooled, hydrolyzed with enzymes and fermented to ethanol. The remaining solids and liquids may be processed further to yield other byproducts. More particularly, the solids may be dried and pressed for use in cattle feed and the liquids may be further fermented or processed to yield additional ethanol, acetate, galacturonic acid monomers and polymers, five carbon sugars and other products.

The invention relates to glucose-rich spirulina polysaccharide and an application thereof in a plant green pesticide and an anti-tumor drug. High-sugar spirulina obtained through nutritional regulation is used as a raw material and is subjected to hot water extraction, protein removal with a flocculant, concentration, precipitation and freeze drying to obtain the water-soluble high-sugar spirulina polysaccharide having the molecular weight of 10-200 kDa; the spirulina polysaccharide comprises the monosaccharides mainly comprising rhamnose, fucose, arabinose, xylose, mannose, galactose, glucose, glucuronic acid and galacturonic acid, and is characterized in that the glucose content is more than 50% in the monosaccharide composition. The spirulina polysaccharide can obviously induce plant disease resistance, has obvious anti-tumor effect on tumor, and can be used as the biological pesticide and the anti-tumor drug.

A method of preventing or treating infection by a range of fungal pathogens in an animal or plant or stored plant product comprising administering an effective amount of a sugar acid, in particular mannonic acid, gluconic acid or galacturonic. The sugar acid can be delivered by a biocontrol that can manufacture the sugar acid. An exemplified biocontrol is Pseudomonas strain AN5, which is characterized by the capacity to convert aldose to sugar acid. Also disclosed are nucleotide sequences from enzymes in the Pseudomonas PQQ enzyme pathway which may be used to transform microorganisms and plants so that they produce sugar acids at levels sufficient to control the growth or viability of pathogens.

Disclosed is a method for improving tobacco extractive incense by multiple biological enzymes, which comprises charging pectolase, cellulose, hemicellulase and beta-glucosidase for enzymolysis, thus producing many kinds of scent generation constituents originally non-existent in the tobacco extract, as well as a plurality of micromolecular carbohydrates and beta-galacturonic acid.

The invention discloses an enzymatic preparation method of low molecular pectin. The method comprises the following steps: carrying out enzymolysis and de-esterification on high molecular high ester pectin by virtue of one or more of pectinase, cellulase, galacturonase and pectinesterase; and carrying out alkali liquor neutralization, filtration and drying and grinding on the product to obtain a pectin product with targeted molecular weight and degree of esterification. According to the method disclosed by the invention, the high molecular high ester pectin is hydrolyzed by adopting a multienzyme compound hydrolyzing technology so as to extract pectin with different molecular weights and degrees of esterification, high content of galacturonic acid and excellent taste from natural pectin. The low molecular pectin powder prepared by the preparation method meets the USP30 standard, various chance elements in the prior art are avoided, and the purity and success rate for extracting the low molecular pectin are improved. Meanwhile, the method is short in process flow, strong in capability of industrialization and suitable for being popularized on a large scale.

The invention belongs to the technical field of plant extracts and particularly relates to a method for extracting pectin from pepper skin slag. The method comprises the process flows: acid-extracting, discoloring, alcohol-precipitating, purifying and drying. The method disclosed by the invention not only can adopt inorganic acid, but also can adopt organic acid to extract, and the organic acid is used for overcoming a pollution problem of inorganic acid extraction on environment; hydrogen peroxide or active carbon is adopted to discolor, so that a problem that the pepper pectin extracting liquor is dark in color and difficult to discolor is solved; alcohol, isopropanol, methanol, acetone and ethyl acetate are used for purifying to sufficiently prepare the pectin with higher purity. According to the method disclosed by the invention, process operation conditions are easy to control, purifying method is simple, pectin yield averagely can reach 9.5%, content of galacturonic acid is not less than 65%, and the pectin belongs to high-ester pectin.

The invention belongs to the field of agricultural product deep processing, and discloses a calcium pectinate production method. According to the method, raw materials are composed of agricultural and sideline product wastes or waste materials, namely citrus pericarp, lemon pericarp and imperfect fruits, shaddock peel, silkworm excrement, mangosteen peel after polyphenol is extracted, dragon fruit pomace and peel, apple pomace and peel, pineapple pomace and peel and the like, the raw materials are subjected to processes of crushing, screening, acid extracting, juice processing, neutralization, calcification flocculent precipitate, dewatering, prilling, drying and the like, and the method can be used for producing jellies, pectin, modified pectin, pectin hydrolysate, galacturonic acid and other calcium pectinate products or calcium supplement health-care food. Compared with an alcohol precipitation method, the flocculation precipitate method in the method is low in energy consumption and cost. Compared with calcium pectinate obtained through the alcohol precipitation, the calcium pectinate produced using the production method is high in biosafety degree and free of toxic and side effects.

The invention relates to a phosphated Arillus Longan polysaccharide which is composed of glucose, arabinose, galacturonic acid, levulose and sorbose. The molecular weight of the phosphated Arillus Longan polysaccharide is 112518.5. The synthesis method of the phosphated Arillus Longan polysaccharide comprises the following steps: (1) separating and extracting Arillus Longan polysaccharide; (2) removing proteins and pigments; (3) carrying out graded purification on the Arillus Longan polysaccharide by a diethylaminoethyl cellulose column; (4) purifying the Arillus Longan polysaccharide by a Sephacryl S-300 HR column; and (5) esterifying the Arillus Longan polysaccharide with a phosphating reagent POCl3. According to the invention, an artificial synthesis method can be used for preparing the phosphated Arillus Longan polysaccharide which contains glucose, arabinose, galacturonic acid, levulose and sorbose.

The invention relates to a hotpot base flavoring, in particular relates to a health base flavoring for hotpot, and belongs to the field of food processing. The health base flavoring contains balsam pear which contains balsam pear glycosides, amino acids (such as glutamic acid, alanine, beta-alanine, phenylalanine, proline, alpha-aminobutyric acid, citrulline, galacturonic acid) and pectin. Accordingly, the base flavoring is nutritious and beneficial to health.

The present invention relates to a feed supplement and a feed containing this feed supplement, comprising 1) a homotrimer, heterotrimer, homotetramer and / or heterotetramer of a carbohydrate component selected from the group consisting of a pentose saccharide, a hexose saccharide, glucuronic and galacturonic acid, and 2) a medium-chain fatty acid (MCFA), selected from the group consisting of caproic acid (C6), caprylic (C8), capric (C10) and lauric acid (C12). The invention also refers to the use of the feed supplement or feed in order to improve the efficiency of animal production, such as weight gain, feed conversion, nutritive value, health and wellbeing through the selective elimination of enteropathogens.

The invention discloses codonopsis pilosula uniform polysaccharide CPPib which is mainly composed of, by Mole percentage, 44.28% of rhamnose, 20.42% of galactose, 11.32% of arabinose and 23.97% of galacturonic acid, weight-average molecular weight of the codonopsis pilosula uniform polysaccharide CPPib is 1.45*105Da, and main carbohydrate chains are 1,2 connected rhamnose (rha, 45%), 1,4-connected galacturonic acid (GalA, 25%) and 1,2,6 connected galactose (Gal, 30%). A preparation method of the codonopsis pilosula uniform polysaccharide CPPib has the advantages of being simple, scientific, reasonable, and capable of preparing products with very high purity with only need of passing through an anion exchanging column chromatograph once. The prepared products have the advantages of having anti-tumor effect, educational effect, very high clinical application value and healthcare function.

The invention provides a processing technology for novel white tea and belongs to the technical field of tea leaf processing. According to the white tea treated through the technology provided by the invention, pathogenic bacteria on the surfaces of the tea leaves can be fully killed and pesticides on the tea leaves can be neutralized through treatment by edible acids, and part of cell walls of the tea leaves are broken, so that nutrients of the white tea are more easily soaked. By spraying a decomposing enzyme to the tea leaves which are treated by the edible acids, the cell walls of the tea leaves are further decomposed, and nutrients such as beta-galacturonic acid, glucose, cellobiose and the like are generated. Through quick cooling in four different stages, the phenol ammonia ratio of the content of the tea leaves can be reduced, the proportion of tea polyphenol showing astringency is reduced, the proportion of free state amino acid compounds showing fresh and smooth characteristics is improved, and meanwhile, volatilization of aromatic substances which are easily volatilized in the white tea such as trans-3-hexenyl alcohol, benzyl alcohol and the like, so that the integral quality of the tea leaves is improved.

The invention discloses a gentian acidity uniform polysaccharide and a purification method and application thereof and belongs to the field of gentian acidity uniform polysaccharide purification. The gentian acidity uniform polysaccharide comprises the following components monosaccharide including, by mole percentage content, 1-1.5% of fucose, 11-12% of rhamnose, 22-23% of arabinose, 17-18% of galactose, 3-3.5% of glucose, 3-3.5% of xylose, 0.5-1% of mannose and 39-40% of galacturonic acid. According to the gentian acidity uniform polysaccharide and the purification method and the application thereof, further separation and purification of the gentian polysaccharide are achieved, the gentian acidity uniform polysaccharide with the weight-average molecular weight of 5.8*104 Da is obtained, the anticoagulant activity of the gentian acidity uniform polysaccharide is high, the preparation method is simple, the hidden danger existing in the residual chemical reagent is avoided, and the gentian acidity uniform polysaccharide has good development application prospect.

The ginseng extracting process capable of separating ginseng polyse from ginseng extract includes the following steps:1. extracting ginseng with 50-85 % concentration alcohol solution to obtain filtrate and filter residue; 2. vacuum drying filtrate to obtain ginseng extract; and 3. extracting the filter residue with water and spraying the water extract to obtain ginseng polyse. The ginseng extracting process has alcohol extraction, water extraction and alcohol precipitation combined organically to produce light brown polyse with effective component galacturonic acid content of 13-30 %, 10 % higher that of traditional process, and best utilization of the side product to obtain ginseng extract.

The invention discloses a method for extracting low-methoxy pectin from apple pomace, comprises the following steps: washing apple pomace by using water at 30-40 DEG.C, microwave treating at 380-500 W and then filtering, microwave drying, the filtered apple pomace, stoving and crushing to obtain apple pomace powder, mixing the apple pomac powder and acid water to perform microwave treatment at 480-500 W, concentrating centrifuged supernatant as concentrated solution, pouring the concentrated solution in an upright filled with active carbon, and collecting the filtrate; heating the filtrate to 5-10 DEG.C, adding alkali solution to regulate the pH value, adding the acid water in the obtained pectin concentration solution to regulate the pH value to 3-5, adding ethanol water in the pectin concentrated solution, mixing, standing and centrifuging to obtain the precipitate, washing by using the ethanol water for 7-8 times, washing, precipitating, vacuum freezing and drying to obtain a substance, namely, the low-methoxy pectin. The process is coherent and simple, the required reagent is reagent in common use, low in cost and easy to purchase; the extracting rate can achieve 7.9-9.4%, the esterfication degree is 38.7-41.4%, and the galacturonic acid content is 72-82%.

Sperm, oocyte, and embryo survival and function is improved in vivo or in vitro by the use of a polysaccharide containing arabinose, galactose and / or hexuronic acid. In particular, a nonspermicidal lubricant containing such a polysaccharide (e.g., gum arabic, pectin, or galacturonic acid) increases the fertilization potential of the sperm during coitus, artificial insemination or sperm collection. Similarly, a freezing medium containing a polysaccharide containing arabinose, galactose and / or hexuronic acid enhances sperm, oocyte, or embryo viability.

The invention discloses a diaphragma juglandis acidic polysaccharose and a preparation and an application thereof. The diaphragma juglandis acidic polysaccharose is prepared from polysaccharide with the weight percent being 99% or above, wherein the polysaccharide comprises galacturonic acid, glucose, galactose and arabinose at the mass ratio of (66.30-72.50) to (13.50-18.60) to (6.20-11.40) to (2.80-4.40); the preparation method of the diaphragma juglandis acidic polysaccharose comprises the following steps: extracting diaphragma juglandis powder crude polysaccharide by water extraction and alcohol precipitation, removing protein by employing an enzyme-Sevage combined technique, dialyzing and purifying by anion exchange chromatography and gel filtration chromatography; and carrying out vacuum freeze-drying, separating and purifying to obtain the diaphragma juglandis acidic polysaccharose. The purified polysaccharide is subjected to component analysis, structural identification and immune function research, so that the diaphragma juglandis acidic polysaccharose has relatively high anti-oxidation activity, can be used as an antioxidant or applied to preparation of the antioxidant, and can be widely applied to cosmetics, food supplements, animal feed additives, medicines and the like.

The invention discloses an extracting method of sisal hemp pectin. The extracting method comprises the following steps: 1, dividing sisal hemp residues into two parts, adding water into the lesser part of the sisal hemp residues, boiling to obtain a first mixed solution, and adding water into the other part of the sisal hemp residues to obtain a second mixed solution; 2, adding galactose into the first mixed solution, inoculating a beer yeast, fermenting to obtain fermentation liquor, adding the fermentation liquor into the second mixed solution, fermenting to obtain secondary fermentation liquor, supplementing galactose during fermentation, cooling the secondary fermentation liquor to obtain a mixed solution, filtering, collecting filtrate, and concentrating the filtrate to obtain a concentrated solution; 3, adding ethanol into the concentrated solution, then performing centrifugal separation, and collecting a precipitate; and 4, air-drying the precipitate, then drying, and crushing to obtain the sisal hemp pectin. According to the extracting method disclosed by the invention, pectin is extracted by adopting a microbiological fermentation method, the extracted pectin is relatively high in molecular mass and high in extraction rate, does not need heat and acid treatments, and is low in consumption and pollution and higher in galacturonic acid content.

The present invention provides a pectic polysaccharide, wherein a degree of methyl esterification of constituent galacturonic acid is 45% or less, a structure of a single molecule observed with an atomic force microscope comprises a star structure, and a diameter of the molecule is more than 100 nm and equal to or less than 200 nm.

The invention relates to the technical field of medicines, and discloses glabrous sarcandra herb acidic polysaccharide quality product SGP-2. The glabrous sarcandra herb acidic polysaccharide is characterized in that the content of polysaccharide is over 90 percent, and the number-average molar mass is 1,000-2,000KDa; monosaccharide comprises glucose, galactose, mannose, arabinose and galacturonic acid; the bond types of glucosidic bond comprise alpha(1->4)galacturonic acid, alpha(1->4) galacturonic acid methyl ester, alpha(1->4) arabinose, alpha(1->4)mannose, beta(1->6) glucose, beta(1->3)galactose, alpha(1->4)glucose, beta (1->4,6)glucose, alpha(1->3,6)galactose, and alpha(1->4,6)galacturonic acid. The invention provides application of the glabrous sarcandra herb acidic polysaccharide quality product SGP-2 in inhibiting alpha-glucosidase activity and application of glabrous sarcandra herb acidic polysaccharide in preparing a medicament for treating diabetes mellitus, and provides application of the glabrous sarcandra herb acidic polysaccharide quality product SGP-2 in preparing anti-tumor medicaments.