102results about How to "Short induction time" patented technology

The invention disclosed provides a method for inducing nucleation in a polymer by subjecting the polymer containing dissolved gas to an external stress generated, for example, by applying hydrostatic or mechanical pressure. The applied stress restricts the bubble growth so that the foamed materials have small cells and high cell density. Such microcellular foams can be produced over a wide low temperature range, i.e. from the temperature at which the polymer is conditioned with the blowing agent up to about the glass transition temperature of the polymer-blowing agent system. Stress induced nucleation can also be conducted at higher temperatures i.e. up to about the Tg of the neat polymer, leading to foams with larger cells. A variety of homogeneous and heterogeneous foams can be produced by this technique.

The invention discloses a method for preparing polyether polyol in a recycling manner by the aid of a DMC (double metal cyanide) catalyst. The method includes carrying out polymerization reaction on first low-molecule alcohol and alkylene oxide to obtain polymers with low molecular weights; carrying out reaction on the polymers with the low molecular weights, DMC, acid and alkylene oxide, simultaneously filling the polymers with the low molecular weights with a certain quantity of second low-molecule alcohol used as a raw material to obtain intermediate target polymers; preparing target polymers from a part of the obtained intermediate target polymers and preparing other intermediate target polymers from another part of the obtained intermediate target polymers in a recycling manner. The first low-molecule alcohol is used as a raw material. The polymers with the low molecular weights are used as initiators, the DMC is used as the catalyst, and the acid is used as an auxiliary. The average molecular weight of the intermediate target polymers is identical to the average molecular weight of the polymers with the low molecular weights. The method has the advantages that the polyether polyol can be prepared by the aid of the method in the recycling manner without alkali manufacturing initiators, and accordingly loss of materials and discharge of filter residues and wastewater can be reduced; the DMC catalyst in reaction systems for initial reaction is already activated under the condition that the concentration of the DMC in the target polymers keeps identical, accordingly, the dehydration time and the initiation time can be shortened to a great extent in production procedures, and the utilization efficiency of devices can be improved.

The invention relates to a culture method for inducing adipose tissue-derived stromal cells to differentiate to chondrocyte, and aims to solve the problem that the prior art is low in differentiation rate. The culture method comprises the following steps: 1) performing separation of primary cells of adipose tissue-derived stromal cells; 2) performing amplification and passage of the adipose tissue-derived stromal cells; and 3) performing differentiation culture on P5-generation adipose-derived stem cells to chondrocyte, namely, adding the adipose tissue-derived stromal cells into a condition culture medium, namely, an adipose tissue-derived stromal cell chondrocyte differentiation culture medium, after P5 generation of passage, performing chondrocyte differentiation culture, replacing the medium of the cells every 3 days, observing the morphological change of the cells, and after 16 days of induction, performing Alcian blue dyeing, and identifying the chondrocyte differentiation situation of the adipose tissue-derived stromal cells. The result of Alcian blue dyeing identification shows that the chondrocyte can be formed, and the Alcian blue dyeing is positive. The culture method has the characteristics of being simple and feasible, short in induction time as the induction culture medium is a serum-free culture system, good in test repeatability and high in osteoblast differentiation rate.

The invention relates to a rapid propagation method for culturing Rieger begonias tissue, which comprises the following steps that: explant material is treated, that is, the explant material is selected for treatment to prepare the explant meeting the requirement; then inducement is carried out, that is, the explant prepared in the first step gets vaccinated to culture medium to induce bud on inducing conditions; the inducing condition that the light intensity with illuminance is less than 1500lx is met. As the lighting condition during the fast propagation of Rieger begonias tissue is further optimized, the application of the rapid propagation method for culturing Rieger begonias tissue causes the inducement time to be shorter and ensures the inducement rate to be higher and reach 100 percent.

The invention discloses a sucrose isomerase gene, namely a pal I gene, which has the nucleotide sequence shown as SEQ ID NO:1. The invention also discloses a coding protein for the gene, namely an SIase enzyme. The invention also discloses an expression vector and a host cell containing the gene, and a high-efficiency expression method for the gene. A recombinant strain containing the sucrose isomerase gene has isomerism activity and can transform sucrose into isomaltulose. The recombinant strain has the advantages of high stability, high catalytic efficiency, obvious improvement of product specificity, and application to industrial production of the isomaltulose.

A Direct Synthesis of making organohalosilanes with greater selectivity to the dialkyldihalosilane is disclosed herein. By using nanosized copper catalyst precursors, and preferably nanosized promoters as well, D / T values of greater than 10, and preferably greater than 15, are obtainable with silicon conversions in excess of 80 wt. %. Shorter induction times are realized using the nanosized copper catalysts in the Direct Synthesis. The nanosized copper catalyst precursors most preferably have an average particle size of less than 100 nanometers.

The invention relates to a tissue culture method of Gentiana dahurica Fisch for rapid in vitro reproduction. The method comprises the following steps: (1) selection and sterilization process of explant, comprising taking the stem tip of seedling of biennial Gentiana dahurica Fisch in a greenhouse as the explant, dipping for sterilization and washing so as to obtain the sterilized explant stem tip; (2) inoculation of explant, comprising inoculating the explant stem tip after sterilization at a callus tissue induction culture medium so as to form callus tissue; (3) proliferation and differentiation culture of the callus tissue, comprising inoculating the callus tissue to a callus tissue proliferation culture medium to culture so as to form single buds of Gentiana dahurica Fisch; (4) induction of root growth, comprising separating the single buds of Gentiana dahurica Fisch and afterwards transplanting into the root growing culture medium to culture so as to form complete regenerated plantlets; (5) sterilization of seedling medium; (6) transplanting of plant, comprising transplanting the complete regenerated plantlets into the seedling substrate subjected to sterilization directly, and acclimating the seedling so as to grow normal Gentiana dahurica Fisch plants. According to the method, the problems of overlong seedling period and low reproduction coefficient of Gentiana dahurica Fisch are overcome, and the method is easy to operate and can be used for rapid factory seedling culture.

The invention relates to a method for synthesizing gas hydrates and a device thereof. The device mainly comprises a snake-shaped heat exchanger with vertical metal fins, a crystal growth pump and a steel gas hydrate reaction tank with heat-insulating coating. After the snake-shaped heat exchanger reduces the temperature inside the tank below a critical decomposition temperature, the crystal growth pump is started to allow the liquid water to pass through the bottom of a liquid refrigerant and enter the refrigerant phase and thereby enhancing the mixture of water and the refrigerant and simultaneously enhancing the heat exchange between the heat exchanger and reactants. At the beginning of gas hydrate synthesis, the crystal growth pump is shut down, so that the gas hydrate grows upward along the vertical metal fins in the refrigerant phase until the whole reactants are used up. The inventive device has the advantages of short induction time for hydrate synthesis, high density of synthetic gas hydrate, high energy-storage density, and good heat exchange performance.

The invention provides a Chuanminshen violaceum tissue culture method. According to the method, the stem tip, tender leaves and fresh tender leaf stalks of the Chuanminshen violaceum plant with good characters in the vigorous growth period are utilized as explants, and the method comprises the following steps of: performing disinfection treatment and inoculation induction to form callus; performing callus multiplication, bud induction culture and rooting culture to obtain complete Chuanminshen violaceum plant seedling; and transplanting the complete seedling into a seedling culture medium, and culturing to obtain a Chuanminshen violaceum annual seedling. All the culture media are based on an MS culture medium in combination with components such as 6-benzyladenine, naphthylacetic acid, 2,4-dichlorphenoxyacetic acid, indolebutyric acid, sucrose an agar. The method provided by the invention can be used for realizing the tissue rapid propagation of Chuanminshen violaceum by use of a plant tissue culture technology, and has important application values in terms of annual seedling supply and improved-variety rapid expanding propagation of the industrialized cultivation of Chuanminshen violaceum.

The invention provides a method for rapid propagation of pinellia ternate by tissue culture. The method comprises the steps as follows: (1) preparation of culture media; (2) selection of explants; (3)obtaining of callus by induction; (4) multiplication culture of callus; (5) differentiation culture of the callus; (6) acceleration culture; (7) strong seedling culture; (8) acclimatization and transplantation. By use of the special culture media and liquid culture of the callus for 10 d, the volume and weight are increased by 300%, and the quality of the callus is better; when green bud points appear in solid culture media, the callus is transferred to liquid culture media again, the callus differentiates after 10 d of culture, a large number of root systems and bud points appear, and the volume and weight are obviously increased; then, the callus is transferred to the solid culture media, root systems continuously grow, the bud points continuously develop to form a large number of leaves, complete pinellia ternate plants can be formed after 15 d, the propagation coefficient reaches 20 or above, the rooting rate reaches 95% or above, and the transplantation survival rate is 99% or above.

The invention discloses an angelica tissue culturing and breeding method, which comprises the following steps: adopting angelica root as external growing bulk, sterilizing, grafting to induce callus, breeding, proceeding bud and root induction, forming entire plant sprout, planting the entire sprout in the substrate, adopting MS culture medium as base and 6-furfuryl adenine, 6-benzyl adenine, 2, 4-dichlorophenoxyacetic acid, fruitone, sucrose and active carbon as auxiliary compositions.

The invention discloses a method for promoting hydration, solidification and separation of a coal mine mash gas mixture, which relates to a method for promoting separation of mash gas. The method solves the problems that the prior method for hydrating, solidifying and separating the coal mine mash gas mixture has long induction time of generating a hydrate of 59.8 percent CH4, low growing rate of a hydrate of 40.4 percent CH4, low concentration of the CH4 in the mash gas mixture, and low separation efficiency. The method comprises the following steps: firstly, adding a separation carrier; secondly, adding an accelerating agent into a reaction kettle, and then pumping out air; thirdly, extracting the mash gas from a coal mine and compressing the mash gas into the reaction kettle to perform the hydration and the solidification; and fourthly, making the hydrates after the hydration and the solidification enter into a storage tank to finish the operation. In the method, the induction time of growing the hydrate of 59.8 percent CH4 is 51 minutes. The concentration of the CH4 in the mash gas mixture is improved by more than 10 percent, and the separation efficiency is high. The growing rate of the hydrate of 40.4 percent CH4 reaches up to 23.03*10 mol per minute.

The invention discloses a butterfly orchid induction culture medium and an asexual propagation method of butterfly orchid. The butterfly orchid induction culture medium is obtained by mixing a standard N6 culture medium serving as basic mother liquor with 6-benzylaminopurine BA, gibberellin GA3 and an organic additive. The butterfly orchid induction culture medium can be used for inducing pedicel internode parts of butterfly orchids to quickly propagate the butterfly orchids and has the advantages of low cost, high protocorm-like body induction rate, short induction time, high growth speed and the like. According to the asexual propagation method of the butterfly orchid, tender pedicel internodes are used as an explant, and the butterfly orchid induction culture medium is used as the culture medium; by the adoption of the method, pedicel materials are fully used; waste is turned into treasure, and the cost is reduced; furthermore, the parent is not damaged; the flowers on the lower parts of the pedicels can be still used for hybridization.

The invention discloses a method for acquiring hemerocallis hybrida regeneration plants by inducing adventitious buds. Hemerocallis hybrida rhizome buds serve as explant materials and are directly induced to generate the adventitious buds, the adventitious buds are split into single plants and subjected to multiplication culture to obtain cluster buds, the cluster buds are split into single plants, the adventitious buds, calluses and redundant basal discs are removed, rooting culture is implemented to obtain rooting seedlings, and seedling hardening and transplanting are implemented to obtainthe hemerocallis hybrida regeneration plants. The method is simple to operate, inducing and breeding time is shortened, induction rate is high, inducing time is short, rooting rate is high, transplanting survival rate is high, the acquired regeneration plants can stably keep excellent characters of female parents, the method overcomes the shortcoming variation of future generation plants by a callus pathway, direct regeneration of tissue culture seedlings of hemerocallis hybrida is achieved, and the method serves as an effective way of industrialized production of the hemerocallis hybrida.

The invention discloses a method for culturing friable embryogenic calluses of manihot esculenta. The method comprises the following steps: (1) inducing the friable embryogenic calluses; (2) regenerating the friable embryogenic calluses. The method for culturing the friable embryogenic calluses of the manihot esculenta has the advantage that friable embryogenic callus induction is short in generation time and high in generation rate; the manihot esculenta is preferably the conventional cultivated varieties such as Huanan 8# and Huanan 6#.

The invention relates to a method for inducing synovium mesenchymal stem cells (SMSCs) to be differentiated to chondrocytes by in-vitro lentivirus mediated BMP-2 (Bone Morphogenetic Protein) genes. The method comprises the following steps of: separating and culturing synovium mesenchymal stem cells; constructing a recombinant plasmid pFUGW-oBMP-2; preparing morbus virosus of transfected lentivirus; and transfecting synovium mesenchymal stem cells by the morbus virosus of transfected lentivirus, wherein in the step of preparing morbus virosus of transfected lentivirus, the transfected lentivirus is jointly formed by the recombinant plasmid pFUGW-oBMP-2 and a packaging plasmid; and in the step of transfecting synovium mesenchymal stem cells by the morbus virosus of transfected lentivirus, synovium mesenchymal stem cells over third generation is taken to be mixed with the morbus virosus of transfected lentivirus, and then added into incomplete chondroblast inducing culture liquid to induce so as to obtain chondrocytes. SMSCs transfected by the transfected lentivirus provided by the invention are safe enough and can be spontaneously differentiated to cartilage in vitro.

The invention relates to a preparation method of a multipotential stem cell-like cell, a composition and an application. The method includes steps of 1), culturing a mononuclear cell in a culture mediu containing a cell growth factor M-CSF, wherein the working concentration of the M-CSF in the culture medium is 10-50 ng / ml, and the culture time is 3-5 days; 2), after step 1), culturing the mononuclear cell in the culture medium containing M-CSF and ophiopogonone to obtain the multipotential stem cell-like cell, wherein the working concentrations of the M-CSF and ophiopogonone in the culture medium are 10-30 ng / ml and 30-100 mu g / ml; the culture time is 4-8 days. Compared with the traditional stem cell induction method, the method has the advantages of being simple in operation, high in inducing efficiency and high in performance cost.

The invention relates to parturition hastening methods for shellfish products, in particular to a method for hastening parturition of shellfishes by sterilized and neutralized sea water. According to the method, outdoor sea water is sedimented in a sedimentation tank, sodium hypochlorite or bleaching powder are added for sterilization, sodium thiosulfate is used for neutralizing residual chlorine in the water, water in a nursery pond is firstly drained off during parturition hastening, the neutralized sea water is injected into the nursery pond until the sea water submerges seed shellfishes, air inflation and oxygenation are performed, the seed shellfishes are added immediately once spawning of the seed shellfishes is discovered, and the sea water is injected until the pond is filled with the sea water. The method is simple, and easy to implement. The method has the advantages of short time, high parturition hastening rate and good effect in terms of shellfish parturition hastening. The method belongs to methods for changing growing environmental conditions of the seed shellfishes, but the method is slow and gentle and does not cause intense stimulation and damage to the seed shellfishes. Indexes such as fertilized-egg hatching rate, metamorphosis rate and blood-shellfish repeating utilization factor are increased by 20% as compared with those in the prior art. The problems of shellfish source shortage and difficulty in seed breeding during shellfish product production are solved indirectly.

The invention provides construction and usage of lactobacillus single-plasmid Nisin inducible expression vector, which belongs to the field of biotechnology. The construction comprises: a, acquisition and identification, the preparation of chromosome DNA producing Nisin lactococcus lactis, the design and synthesis of primer, the PCR amplification, recovery, pGEM-T connection and transformation ofa nisRK gene, and the identification of the nisRK gene; and b, the construction of pW425N, the recovery, connection and identification of the nisRK gene and a basic vector pW425et, the acquisition and identification of inducible promoter nisA gene, and the construction of pW425N. The usage comprises: a, the acquisition and identification of a green fluorescent protein gfp gene; b, the construction and identification of a recombinant expression vector; c, the determination of the inducible expression function of a Nisin inducible expression vector for green fluorescent protein; and d, the detection of lactobacillus stability. The invention has the advantages of avoiding the troublesome operation of integrating chromosomes of host bacteria in the prior system and simplifying the construction of recombinant genetic-engineering lactic acid bacteria.

The invention relates to a lilium pumilum somatic embryo direct generation method with effect of significantly shortening induction time. The method comprises the following steps: inoculating asepticseedlings of lilium pumilum into an MS medium with 60g.L<-1> sucrose, culturing the seedlings for 45 days, taking outer scale, inoculating the outer scale into a somatic embryo induction medium, culturing the outer scale in dark for 30 days to obtain globular embryos; transferring a culture containing the globular embryos to a somatic embryo proliferation medium, performing culturing for 25 days to obtain secondary proembryos, and performing multiplication in the form of secondary somatic embryos; transferring the secondary proembryos to the MS medium, performing culturing in light for 20 daysfor germination into seedlings. The lilium pumilum somatic embryos are obtained in a direct regeneration way for the first time, the problem of long embryo induction cycle is effectively solved, obtaining time of the globular embryos and the final tissue culture seedling obtaining time is shortened by 90 days, the method has the advantages of stable explant source, simple and implementable stepsand high repeatability, and an efficient and stable regeneration system can be provided for rapid propagation, germplasm preservation and genetic transformation of the lilium pumilum.

The invention relates to the technical field of establishment of an animal model used for screening drug and researching disease mechanism, in particular to a screening method of a febrile convulsion model rat, which can be used for obtaining the model rat by screening in a way of combining a typical water bath heating induction model with artificial directional selection and mating breeding. Themethod is unique and can be used for successfully obtaining the good febrile convulsion sensitive model rat and a tolerance model rat.

The invention provides a method for rapidly culturing and producing galanthamine, lycorine and lycoramine by rapid propagation of bulblets of lycoris. The method comprises the following steps: taking a bulb of 'lycoris chinensis' as an explant, sterilizing, inoculating and culturing for a period of time, generating bulblets at a wound of a scale, and continuing carrying out multiplication culture on the bulblets; and drying tissues of the bulblets of the lycoris chinensis, grinding and ultrasonically extracting with methanol for three times to obtain a methanol solution containing the galanthamine, the lycorine and the lycoramine. The problems that resources of lycoris herbs are in shortage, and chemical synthesis of the galanthamine, the lycorine and the lycoramine is complicated are solved by a tissue culturing technology. According to a used basic medium, an SH medium is used as a base, and ingredients such as 6-benzylamino adenine, alpha-naphthylacetic acid and a yeast extract are used as auxiliary ingredients; and the method lays a foundation for industrial production of the galanthamine, the lycorine and the lycoramine and is environment-friendly, raw materials are abundant, and the supply gap of markets to the galanthamine, the lycorine and the lycoramine can be relieved well.

The invention provides a method for extracting paclitaxel from induction of taxus chinensis var. mairei axillary bud germination in vitro and then cultivation and production. On the basis of a WPM (woody plant medium) minimal medium, the method uses (800-1000) mg / L of (NH4)2SO4 to replace 990 mg / L of K2SO4 to obtain the improved WPM minimal medium, and adding 0.01-4.5 mg / L of TIBA; pruning annualyoung shoots from a taxus chinensis var. mairei tree, cutting into small stem sections in 2.5cm, sterilizing, inoculating to the improved WPM minimal medium, and performing alternative culture, namely light culture and dark culture, in an artificial climate box for culturing temperature, wherein the temperature is 25+ / -1 DEG C in the daytime and is 20+ / -1 DEG C in the night, the illumination intensity is 1500lx, and relative humidity is 80%, thus rapidly and successfully inducing axillary buds based on the WPM plus 0.01-4.5 mg / LTIBA. By using the method, the industrialization of the taxus chinensis var. mairei axillary buds can be realized with large scale, short period, high reproduction rate and low cost. Buds can be induced around 15 days with 100% induction rate; and the taxus chinensis var. mairei axillary buds induced by the method is thick and short and has freshly green color. The content of paclitaxel in buds obtained by the method can reach 0.0042% in fifty days, which is higher than that of paclitaxel in natural buds.

The invention provides a method for preparing a rejuvenation culture medium for the tissue culture of high-bush blueberry. The method comprises the following steps: (1) treating explants materials, i.e., selecting and treating explants materials, and preparing explants satisfying the requirements at the balanced point of the minimal killing rate and contamination rate; (2) inducing, i.e., inoculating the explants in step (1) on a culture medium, and inducing the explants to bud under the condition of inducing, wherein the culture medium is particularly an improved agar synthetic medium; and (3) carrying out the rejuvenation culture, i.e., carrying out the enrichment culture on the shoots of the cluster buds obtained in step (2) in a rejuvenation culture medium.

The invention relates to a culture medium for promoting Vibrio parahemolyticus to enter non-culturable state and a method, belonging to the field of microorganism separation culture. The formula of the culture medium for promoting Vibrio parahemolyticus to enter non-culturable state comprises the following components in each 1000mL culture medium: 5-15g peptone, 1-5g beef extract, 20-40g sodium chloride, and 0.1-1g potassium sorbate. The method comprises the following steps: adding distilled water till 1000mL; adjusting the pH to be 5-8; vaccinating vibrio parahemolyticus in logarithmic phase into the culture medium; stewing at the temperature of -10 to -20 DEG C; inducing for 105h and then the vibrio parahemolyticus enters the non-culturable state. The culture medium for promoting vibrio parahemolyticus to enter non-culturable state provided by the invention is simple to prepare, so that the induction time of vibrio parahemolyticus entering the non-culturable state is short, and the scientific research cost is greatly saved.

The invention discloses a compound inducing culture medium. The compound inducing culture medium contains three differentiation culture solutions AD, DB and DC. A method for inducing umbilical cord mesenchymal stem cells into neuron-like cells by virtue of the compound inducing culture medium comprises the following steps: pre-inducing the umbilical cord mesenchymal stem cells for 2 days by virtue of the differentiation culture solution DA; carrying out differentiation culture for 1 day by virtue of the differentiation culture solution DB; finally, carrying out maintenance culture for 1 day by virtue of the differentiation culture solution DC; and observing the morphological characteristics of the induced neuron-like cells, and detecting the mRNA level of induced neural differentiation relevant genes and the expression conditions of neuron migration proteins DCX and neuron specific enolase NSE which induce the neuron-like cells, wherein positive subjects of DCX expression are neural precursor cells, and positive subjects of NSE expression are the neuron-like cells. According to the method, the induction process is divided into three stages, and different culture mediums are adopted in each stage, so that the induction time is short, the induction efficiency is high, and the induced differentiated neuron-like cells can stably live and have no repellence after being transplanted.

The invention discloses an establishment method of an apoptosis model for inducing pig small intestinal epithelial cells by hydrogen peroxide. The establishment method comprises the following steps: preparing a cell culture medium; inoculating the pig small intestinal epithelial cells into the cell culture medium for culturing, and carrying out trypsin digestion treatment to obtain to-be-induced cells; and adding the cell culture medium containing the hydrogen peroxide into the to-be-induced cells and carrying out hydrogen peroxide induction. In the technical scheme provided by the invention,a cell apoptosis model is established by taking a pig small intestinal epithelial cell as a model cell for the first time and the hydrogen peroxide is used as an inducer; the establishment method hasthe advantages of short induction time, simple operation, low cost and stable and reliable effects; and the establishment of the cell apoptosis model provides an important way to study a pig intestinal injury mechanism and regulation measures thereof.

The invention discloses oxygen scavenging films having at least one oxygen scavenging layer comprising a blend of ethylene / methyl acrylate / cyclohexene methyl acrylate copolymer (EMCM) as oxygen scavenger resin and a catalyst in a carrier resin, and an outer substrate layer having a thickness greater than 5% with respect to the total thickness of the film. The invention also relates to a process for the manufacturing of such films, to the use of said films in food packaging and to the packages obtained therefrom.

The invention discloses an auditory sense attention characteristic extraction and recognition system and method based on middle latency auditory evoked potential. The system comprises an equipment control module, a data storage unit, a stimulus sound generating device, a data acquisition device and a data processing and analyzing module. The stimulus sound generating device, the data acquisition device and the data processing and analyzing module are connected with the equipment control module, and the data storage unit is connected with the equipment control module, the data acquisition device and the data processing and analyzing module. Effective event related potential can be induced, and the energy, variance, area, AR model coefficient and waveform peak value are calculated as characteristic values. Finally, classification is carried out through a mode recognition algorithm. The experimental result shows that the average accuracy of eight testees with an artificial neural network (ANN) as a classifier can reach 77.2%. The experimental scheme of the design is convenient, simple and effective.