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Method for preparing rejuvenation culture medium for tissue culture of high-bush blueberry

A medium preparation and tissue culture technology, applied in the field of bioengineering, can solve the problems of long induction time, difficult rooting, low survival rate, etc., and achieve good effect and convenient induction culture

Inactive Publication Date: 2010-09-22
山东高端蓝莓生物技术股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art generally uses the traditional subculture medium, resulting in weak subculture buds; and the induction time is longer, the rooting is difficult, the survival rate is low, and it cannot adapt to the faster market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Treatment of explant materials: including cleaning, sterilizing and cutting the explant materials to prepare explants that meet the requirements.

[0028] Specifically: use the tender stems from which the leaves are cut from the current year, rinse with tap water, rinse 3-5 times with sterile water on a sterile workbench, rinse with tap water for 20-30 minutes, and then soak in water with detergent Wash for 20 to 30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant added with activated carbon for 10 minutes, and put it into a sterile bottle for use after disinfection.

[0029] 2. Induce budding: inoculate the explants in the medium with the formula of agar comprehensive medium + zeatin 0.8 mg / L + naphthalene acetic acid 0.2 mg / L, inoculate the explants on the induction medium, and start after 1 week. Germination, after 40 days, the buds grow into new shoots of about 4-5cm, and the ...

Embodiment 2

[0032] 1. Treatment of explant materials: including cleaning, sterilizing and cutting the explant materials to prepare explants that meet the requirements.

[0033] Specifically: use the tender stems from which the leaves are cut from the current year, rinse with tap water, rinse 3-5 times with sterile water on a sterile workbench, rinse with tap water for 20-30 minutes, and then soak in water with detergent Wash for 20 to 30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant added with activated carbon for 20 minutes, and put it into a sterile bottle for use after disinfection.

[0034] 2. Induce budding: inoculate the explants in the medium with the formula of agar comprehensive medium + zeatin 1.0 mg / L + naphthalene acetic acid 1.0 mg / L, inoculate the explants on the induction medium, and start after 1 week. Germination, after 45 days, the buds grow into new shoots of about 4-5cm, and the ...

Embodiment 3

[0037] 1. Treatment of explant materials: including cleaning, sterilizing and cutting the explant materials to prepare explants that meet the requirements.

[0038] Specifically: use the tender stems from which the leaves are cut from the current year, rinse with tap water, rinse 3-5 times with sterile water on a sterile workbench, rinse with tap water for 20-30 minutes, and then soak in water with detergent Wash for 20 to 30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant added with activated carbon for 30 minutes, and put it into a sterile bottle for use after disinfection.

[0039] 2. Induce budding: inoculate the explants in the medium with the formula of agar comprehensive medium + zeatin 1.3 mg / L + naphthalene acetic acid 1.6 mg / L, inoculate the explants on the induction medium, and start after 1 week. Germination, after 52 days, the buds grow into new shoots of about 4-5cm, and the ...

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PUM

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Abstract

The invention provides a method for preparing a rejuvenation culture medium for the tissue culture of high-bush blueberry. The method comprises the following steps: (1) treating explants materials, i.e., selecting and treating explants materials, and preparing explants satisfying the requirements at the balanced point of the minimal killing rate and contamination rate; (2) inducing, i.e., inoculating the explants in step (1) on a culture medium, and inducing the explants to bud under the condition of inducing, wherein the culture medium is particularly an improved agar synthetic medium; and (3) carrying out the rejuvenation culture, i.e., carrying out the enrichment culture on the shoots of the cluster buds obtained in step (2) in a rejuvenation culture medium.

Description

Technical field: [0001] The invention belongs to the technical field of bioengineering, relates to plant tissue culture technology, and in particular relates to the preparation and application of a high-bush blueberry industrial tissue culture fast-propagating and rejuvenating medium. Background technique: [0002] High-bush blueberry (Blueberry), scientific name bilberry, is a perennial shrub native to North America. Generally, seeds cannot be formed, and most of the propagation methods such as cuttings and layering are used. However, high-bush blueberries have strict requirements on temperature, soil, and climate. , the quality of cutting propagation is not ideal. The technology of tissue culture propagation of plants is a special way of vegetative propagation, which is now generally called rapid propagation technology or micropropagation technology. It is a method of multiplying plants under artificially controlled nutritional and environmental conditions using parts of...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 不公告发明人
Owner 山东高端蓝莓生物技术股份有限公司
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