Process for industrialized tissue culture and rapid propagation of high-bush blueberry
A rapid propagation and high irrigation technology, applied in the field of bioengineering, can solve the problems of long induction time, low survival rate, low induction rate of emergence, etc., and achieve the effect of convenient induction and cultivation and good effect
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Embodiment 1
[0027] 1. Treatment of explant materials: including cleaning, disinfection and cutting of explant materials to prepare explants that meet the requirements.
[0028] The specific method is: use the tender stems of the current year with the leaves cut off, rinse them with tap water, rinse them with sterile water 3-5 times on a sterile workbench, rinse them with tap water for 20-30 minutes, and then soak them in water with detergent Wash for 20-30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant solution with activated carbon for 10 minutes, and put it into a sterile bottle for later use after disinfection.
[0029] 2. Induce germination: Inoculate the explants in a medium formulated as agar comprehensive medium + zeatin 0.8 mg / L + naphthalene acetic acid 0.2 mg / L, inoculate the explants on the induction medium, and start after 1 week After germination, after 40 days, the buds grow into new sh...
Embodiment 2
[0032] 1. Treatment of explant materials: including cleaning, disinfection and cutting of explant materials to prepare explants that meet the requirements.
[0033] The specific method is: use the tender stems of the current year with the leaves cut off, rinse them with tap water, rinse them with sterile water 3-5 times on a sterile workbench, rinse them with tap water for 20-30 minutes, and then soak them in water with detergent Wash for 20-30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant solution with activated carbon for 20 minutes, and put it into a sterile bottle for later use after disinfection is completed.
[0034] 2. Induce germination: inoculate the explants in a medium formulated as agar comprehensive medium + zeatin 1.0mg / L + naphthaleneacetic acid 1.0mg / L, inoculate the explants on the induction medium, and start after 1 week After germination, after 45 days, the buds grow i...
Embodiment 3
[0037] 1. Treatment of explant materials: including cleaning, disinfection and cutting of explant materials to prepare explants that meet the requirements.
[0038] The specific method is: use the tender stems of the current year with the leaves cut off, rinse them with tap water, rinse them with sterile water 3-5 times on a sterile workbench, rinse them with tap water for 20-30 minutes, and then soak them in water with detergent Wash for 20-30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant solution with activated carbon for 30 minutes, and put it into a sterile bottle for later use after disinfection is completed.
[0039] 2. Induce germination: Inoculate the explants in a medium formulated as agar comprehensive medium + zeatin 1.3mg / L + naphthaleneacetic acid 1.6mg / L, inoculate the explants on the induction medium, and start after 1 week After germination, after 52 days, the buds grow i...
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