Method for acquiring hemerocallis hybrida regeneration plants by inducing adventitious buds

A technology of Hemerocallis grandiflora and regenerated plants, which is applied in the field of plant tissue culture, to achieve the effects of facilitating rooting, preventing browning, and increasing the content of

Inactive Publication Date: 2019-05-07
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the number of chromosomes, DNA content, and the proportion of DNA polymorphic fragments will vary to varying degrees with the increase in the number of subcultures of plants obtained through the callus approach, and the research report of using Hemerocallis grandiflorum tissue to directly induce adventitious buds rarely

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  • Method for acquiring hemerocallis hybrida regeneration plants by inducing adventitious buds

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Embodiment 1

[0039] Embodiment 1 induces adventitious buds and obtains the method for Hemerocallis grandiflora regeneration plant, comprises the following steps:

[0040] 1) Select explants, pretreatment

[0041] Select the root shoots of Hemerocallis grandiflora with a length of 1-2 cm and not yet unearthed as explants, wash the soil, cut off all the roots, peel off the outermost 2-3 layers of leaves, wash and soak with detergent About 20 minutes, rinse under running water for 2-3 hours. On the ultra-clean workbench, first treat the explants with 75% alcohol for 30 seconds, rinse them with sterile water three times, and dry them with sterile filter paper; Wash 5 times, each time for 1-2min; then blot the water on the surface of the explant with sterile filter paper, cut off the outermost layer of tissue at the base, and set aside.

[0042] 2) Induce adventitious buds

[0043] The pretreated explants were equally divided into 3 small pieces, inoculated on the induction medium for induct...

Embodiment 2

[0056] Embodiment 2 industrialization method for breeding Hemerocallis grandiflora, comprises the following steps:

[0057] 1) Select explants, pretreatment

[0058] Select the unearthed Hemerocallis grandiflora rhizome sprouts with a length of 1-2cm as explants, wash the soil, cut off all the roots, peel off the outermost 2-3 layers of leaves, wash and soak with detergent for 20 minutes Left and right, rinse under running water for 2 to 3 hours. On the ultra-clean workbench, first treat the explants with 75% alcohol for 30 seconds, rinse them with sterile water three times, and dry them with sterile filter paper; Wash 5 times, each time for 1-2min; then blot the water on the surface of the explant with sterile filter paper, cut off the outermost layer of tissue at the base, and set aside.

[0059] 2) Induce adventitious buds

[0060] The pretreated explants are equally divided into 2 small pieces, inoculated on the induction medium for induction and culture for 30 days, an...

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Abstract

The invention discloses a method for acquiring hemerocallis hybrida regeneration plants by inducing adventitious buds. Hemerocallis hybrida rhizome buds serve as explant materials and are directly induced to generate the adventitious buds, the adventitious buds are split into single plants and subjected to multiplication culture to obtain cluster buds, the cluster buds are split into single plants, the adventitious buds, calluses and redundant basal discs are removed, rooting culture is implemented to obtain rooting seedlings, and seedling hardening and transplanting are implemented to obtainthe hemerocallis hybrida regeneration plants. The method is simple to operate, inducing and breeding time is shortened, induction rate is high, inducing time is short, rooting rate is high, transplanting survival rate is high, the acquired regeneration plants can stably keep excellent characters of female parents, the method overcomes the shortcoming variation of future generation plants by a callus pathway, direct regeneration of tissue culture seedlings of hemerocallis hybrida is achieved, and the method serves as an effective way of industrialized production of the hemerocallis hybrida.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for obtaining regenerated plants of Hemerocallis grandiflorum by inducing adventitious buds. Background technique [0002] Hemerocallis (Hemerocallis), also known as golden needle, forget worry grass, day lily, Yiman grass, cure sorrow, etc., is a perennial herbaceous flower of the genus Hemerocallis in the family Liliaceae. It has the advantages of beautiful shape, various colors, robust growth, and wide adaptability. , flower beds, and important flowers in street greening. [0003] Hemerocallis fulva, also known as polyploid Hemerocallis fulva, is a processed polyploid dwarf variety based on Hemerocallis fulva. The propagation methods of Hemerocallis mainly include seedling method, stem bud cutting method, division method and tissue culture method. [0004] The seedlings produced by sowing are more suitable for colder climates, but the produc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨柳燕王艺程张永春蔡友铭
Owner SHANGHAI ACAD OF AGRI SCI
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