Culture medium for promoting vibrio parahemolyticus to enter non-culturable state and method

A technology of Vibrio hemolyticus and culture medium, applied in the field of microbial isolation and culture, can solve the problems of increasing scientific research costs, delaying scientific research progress, etc., and achieves the effects of saving scientific research costs, short induction time, and simple preparation

Inactive Publication Date: 2013-04-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of scientific research, the artificial cultivation of a live non-culturable state is the primary factor. The existing research results show that using low temperature combined with oligotrophic conditions to induce normal Vibrio parahaemolyticus into a live non-culturable state requires For half a month or even longer, this will undoubtedly increase the cost of scientific research and slow down the progress of scientific research

Method used

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  • Culture medium for promoting vibrio parahemolyticus to enter non-culturable state and method
  • Culture medium for promoting vibrio parahemolyticus to enter non-culturable state and method
  • Culture medium for promoting vibrio parahemolyticus to enter non-culturable state and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of culture medium to promote Vibrio parahaemolyticus into a viable non-culturable state

[0024] Weigh 10g of peptone, 3g of beef extract, 30g of sodium chloride, and 0.1g of potassium sorbate, add distilled water to 1000mL, adjust the pH to 7.4 with 1M HCl and 1M NaOH, and sterilize at 121°C and 1.05MPa for 15 minutes to obtain a parahemolytic arc Bacteria enter a liquid medium in a viable, non-culturable state.

[0025] Weigh 10g of peptone, 3g of beef extract, 30g of sodium chloride, 0.1g of potassium sorbate, 15g of agar, add distilled water to 1000mL, adjust the pH to 7.4 with 1M HCl and 1M NaOH, and sterilize at 121°C and 1.05MPa for 15 minutes to promote parahemolysis Vibrio enters solid media in a viable non-culturable state.

[0026] (2) Rapid induction of a viable non-cultivable state of Vibrio parahaemolyticus

[0027] 1) The standard strain of Vibrio parahaemolyticus GMCC1.1614 (purchased from China General Microorganism Culture Collection ...

Embodiment 2

[0034] (1) Preparation of culture medium to promote Vibrio parahaemolyticus into a viable non-culturable state

[0035] Weigh 10g of peptone, 3g of beef extract, 30g of sodium chloride, 1g of potassium sorbate, add distilled water to 1000mL, adjust the pH to 7.4 with 1M HCl and 1M NaOH, and sterilize at 121°C and 1.05MPa for 15min to obtain Vibrio parahaemolyticus Liquid culture medium that enters a living, non-culturable state.

[0036] Weigh 10g of peptone, 3g of beef extract, 30g of sodium chloride, 1g of potassium sorbate, 20g of agar, add distilled water to 1000mL, adjust the pH to 7.4 with 1M HCl and 1M NaOH, and sterilize at 121°C and 1.05MPa for 15min to obtain a parahemolytic arc Bacteria enter a viable, non-cultivable state on a solid medium.

[0037] (2) Rapid induction of a viable non-cultivable state of Vibrio parahaemolyticus

[0038] According to the method of Example 1 (2), the liquid medium prepared in Example 2 was used for induction at -20°C, and the resul...

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Abstract

The invention relates to a culture medium for promoting Vibrio parahemolyticus to enter non-culturable state and a method, belonging to the field of microorganism separation culture. The formula of the culture medium for promoting Vibrio parahemolyticus to enter non-culturable state comprises the following components in each 1000mL culture medium: 5-15g peptone, 1-5g beef extract, 20-40g sodium chloride, and 0.1-1g potassium sorbate. The method comprises the following steps: adding distilled water till 1000mL; adjusting the pH to be 5-8; vaccinating vibrio parahemolyticus in logarithmic phase into the culture medium; stewing at the temperature of -10 to -20 DEG C; inducing for 105h and then the vibrio parahemolyticus enters the non-culturable state. The culture medium for promoting vibrio parahemolyticus to enter non-culturable state provided by the invention is simple to prepare, so that the induction time of vibrio parahemolyticus entering the non-culturable state is short, and the scientific research cost is greatly saved.

Description

technical field [0001] The invention relates to the field of microorganism isolation and cultivation, in particular to a culture medium and an induction method for promoting Vibrio parahaemolyticus to enter a living non-culturable state. Background technique [0002] Vibrio parahaemolyticus (Vibrio parahaemolyticus) is an important member of the microbial system in oceans and salt lakes. It mainly exists in seawater, submarine sediment sludge, fish and shellfish products and salted food. It is an important food-borne pathogen. . This bacterium has a strong survival ability and can survive for more than 1 month on rags and cutting boards, and can survive in seawater for 47 days. Clinically, the main symptoms are abdominal pain, vomiting, diarrhea and watery stools, which mostly occur in coastal areas in summer and autumn, and often cause collective illness. In recent years, due to the air transportation of seafood, the number of cases in inland cities has gradually increase...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/63
Inventor 王丽钟青萍陈颖翘
Owner SOUTH CHINA AGRI UNIV
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