The invention provides a method for separating and culturing human
umbilical cord mesenchymal stem cells, which comprises the following steps: taking
umbilical cord of
fetus born after the normal period of
gestation by
caesarean section, flushing by PBS under aseptic condition, removing the bloodstain; using an aseptic scissor in an animal
cell medium to
cut the washed
umbilical cord to tissue blocks, removing
blood vessel and crushing, then fully mixing with the animal
cell medium and oscillating, centrifuging, mixing the centrifuged deposition and a MesenCult-XF complete medium and oscillating, and uniformly planting in a
cell culture dish, placing in an
incubator with the temperature of 37 DEG C and 5% of CO2 for culturing for 5 days, sucking the tissue blocks out and discharging, refilling the MesenCult-XF complete medium, and replacing the medium
every three days for continuously culturing to obtain the umbilical cord mesenchymal stem cells. According to the invention, the animal serum is used, the operation is simple, the
repeatability is good, the security is high, and the obtained cell enables stabilization through multitime passage.