62 results about "Embryonic Tissue" patented technology

The present disclosure describes mammalian pluripotent embryonic-like stem cells (ELSCs) isolated from corneal limbal tissue, a non-embryonic tissue. The ELSCs of the present disclosure are capable of proliferating in an in vitro culture, maintain the potential to differentiate into cells of endoderm, mesoderm, and ectoderm lineage in culture, and are capable of forming embryoid-like bodies when placed in suspension culture. Thus, these cells possess multi-lineage differentiation potential and self-renewing capability. ELSCs may be a promising therapeutic tool, and may provide new therapeutic alternatives for various diseases, conditions, and injuries.

A stem cell which is obtainable from the non-embryonic tissue isolated from the dental follicle of tooth or wisdom tooth which are able to differentiate into a periodontal ligament like membrane structure.

The present invention provides methods and compositions for establishing and maintaining growth of cells and embryonic tissue on a synthetic polymer matrix. For example, the present invention provides synthetic growth matrices for stem cells, gametes, mature differentiated cells, and embryonic tissue (e.g., blastomeres, embryos, and embryoid bodies). In certain embodiments, the cells are capable of going through multiple passages while remaining in an undifferentiated state as a result of the synthetic polymer matrix.

The present disclosure describes the generation of neural cells and neurons from mammalian pluripotent embryonic-like stem cells (ELSCs) isolated from corneal limbal tissue, a non-embryonic tissue. Specifically, the present disclosure describes the generation of neuroprogenitor cells and differentiated dopaminergic neurons from ELSCs. The disclosed methods demonstrate the potential of ELSCs as a therapeutic tool, and may provide new therapeutic alternatives for various diseases, conditions, and injuries. Neuroprogenitor cells generated from ELSCs isolated from corneo-limbal tissue were transplanted into a rat model of Parkinson's disease, and were able to alleviate motor abnormalities in the rats for at least six months.

The invention discloses a method for extracting histocytes from large Michelia alba lateral buds, and relates to the technical field of biological histocyte extraction. The method comprises the following steps: 1, plant culturing; 2, bud cultivation; 3, lateral bud cutting; 4, lateral bud propagation growth; 5, lateral bud growing point embryonic tissue extraction; 6, extraction and purification;and 7, extraction component analysis. Large Michelia alba V3 variety flowered plant segment stalk buds undergo tissue culture, and newborn cells cut from the lateral buds undergo high frequency extraction at 50-60 DEG C in an ultrasonic device with pure water as a solvent.

The invention discloses a disposable uterine cavity tissue aspirator which comprises a needle cylinder, a connection device and a suction tube. The connection device is provided with a first port, a second port and a third port. The second port and the third port of the connection device are internally provided with a first one-way valve and a second one-way valve respectively; the first one-way valve only allows the second port to input air, and the second one-way valve only allows the third port to output air; the needle cylinder is connected with the first port of the connection device; the rear end of the suction tube is connected with the second port of the connection device, and the front of the suction tube is provided with an inlet. The disposable uterine cavity tissue aspirator is simple in structure, low in cost, capable of being used in a disposable mode and free of the problem of cross infection generated due to the fact that the disposable uterine cavity tissue aspirator is not cleaned completely or sterilized strictly; it is convenient for a doctor to search for the embryonic tissue in the uterine cavity or pick the uterine cavity tissue; the negative pressure in the suction tube can be controlled by controlling the pulling speed of the needle cylinder by the doctor according to requirements, and operation is quite convenient.

The invention discloses a gum tree embryonic tissue long term fertilizing method that includes selecting and processing heir embryonic tissue, fertilizing process of heir embryonic tissue, restoring process of heir embryonic, and inducing maturing process of embryonic tissue after inheriting. The invention has the advantages of simple technology flow, low producing cost, and high fertilizing of embryonic tissue, and long time of inheriting. It has crucial application values.

The invention relates to an embryonic tissue segmentation method based on a generative adversarial network, and belongs to the technical field of medical image processing. The method comprises the steps of step 1, performing tissue segmentation mask mapping on an embryonic tissue slice image through a U-net network; step 2, making a segmentation network training set; step 3, configuring parametersrequired by network training to obtain a set network; step 4, training the set organization quality identification network by using the manufactured segmentation network training set; step 5, fixingparameters of the organization quality identification network, and training the set U-net network by using the manufactured segmentation network training set in combination with the organization quality identification network; and step 6, taking the embryonic tissue slice image without the marked segmentation result as input, and generating a corresponding mask image. The network relied on by thesegmentation method uses a classification model to supplement loss during training and segmentation, fully utilizes the information of the cell growth state, and improves the accuracy of the segmentation network in the field of embryo tissue segmentation.

The invention discloses an embryoid induction method taking a polyphylla bud axis as an explant. The method comprises the following steps: firstly, carrying out disinfection and pre-cultivation on an un-germinated bud on polyphylla rhizome, and then cutting off coleoptile of the bud of which the volume obviously expands after pre-cultivation; inserting the bud axis into a callus-inducing medium to cultivate from the center after cutting in length, and then cultivating the callus tissue generated by the bud axis to obtain polyphylla embryonic tissues; finally carrying out induction cultivation on the embryonic tissues, so as to generate white spherical embryoids. By adopting the embryoid induction method, a new path is supplied for cultivation of excellent variety of the polyphylla and massive and rapid propagation of tissue culture seedlings; mass production of polyphylla plants at low cost within a short period of time can be achieved.

The invention discloses a trachemys scripta elegans embryo fibroblast cell line and a construction method thereof. The construction method comprises the following steps: taking a trachemys scripta elegans egg hatched for 12 to 18 days, selecting a live embryo and then sterilizing the trachemys scripta egg; shelling, taking the trachemys scripta embryo, discarding the head, fours limbs and visceraof the trachemys scripta embryo, and flushing the rest embryonic tissues with a PBS (Phosphate Buffered Saline) containing double-antibody until no obvious bloodstain is found; using trypsin to digestthe embryonic tissues which are flushed until no obvious bloodstain is found, collecting digested monoplast, and inoculating the monoplast into a complete medium for static culture; when the cells are partially adherent and do not float by slightly oscillating, absorbing the complete medium and the cells which are not adhered, and adding the rest adherent cells into a new complete medium for continuously culturing; when the confluence of primary cultured cells reaches 90 percent or above, digesting with the trypsin, collecting the monoplast and then inoculating into the complete medium for subculture. The invention lays a material and technical foundation for further using RSTEFs for conducting basic theory and experimental research.

The present invention provides a supplement and a culture media useful for culturing mammalian gametes and embryonic tissue. The culture media comprises at least one of recombinant human albumin, fermented hyaluronan, and citrate. Because the constituents are produced from non-conventional sources, the culture medium is free from contaminants such as viruses, prions and endotoxins. Additionally, because the medium is completely defined, the medium is not subject to variations which can impair the development of mammalian cells and prevent meaningful comparisons of empirical studies.

The present invention provides methods and compositions for establishing and maintaining growth of cells and embryonic tissue on a synthetic polymer matrix. For example, the present invention provides synthetic growth matrices for stem cells, gametes, mature differentiated cells, and embryonic tissue (e.g., blastomeres, embryos, and embryoid bodies). In certain embodiments, the cells are capable of going through multiple passages while remaining in an undifferentiated state as a result of the synthetic polymer matrix.

The invention discloses a propagation method for a pseudotsuga sinensis dode plant through somatic embryogenesis. The propagation method comprises the following steps that embryonic tissue induction culture, multiplication culture, embryonic tissue adjustment culture, somatic embryo (somatic embryo) maturation culture, mature somatic embryo germination culture and seedling transfer culture are carried out on a sterile explant of the pseudotsuga sinensis dode plant. Through the method, the stable pseudotsuga gaussenii flous embryogenic cell strain and a large number of mature somatic embryos are obtained through a somatic embryogenesis induction way for the first time, so that a complete somatic embryo plant is obtained, by using the method, a large number of pseudotsuga gaussenii flous somatic embryo seedlings can be produced in a relatively short period, and the technology is suitable for large-scale and industrialized production of the cloned pseudotsuga gaussenii flous seedlings.

The invention discloses a primary culture method of the embryonic cell tissue pieces of a Chinese softshell turtle. The primary culture method comprises the following steps of: (1) collecting the fertile eggs of the Chinese softshell turtle, and disinfecting; (2) breaking the eggshells of the fertile eggs, separating embryos from yolk, cleaning the separated embryonic tissues with a 1*PBS (Phosphate Buffer Solution), and then soaking for sterilization; (3) cleaning the embryonic tissues for 4-5 times in an M199 culture medium, then transferring into a complete culture medium, cutting the embryonic tissues into pieces to obtain tissue pieces, and then cleaning the tissue pieces once by using the complete culture medium; (4) uniformly dispersing the tissue pieces to the inner wall of a cell bottle by using a sterile disposable inoculating needle so that the tissue pieces grow for 3-4 hours in a way of being attached to the wall, then adding the complete culture medium to submerge the tissue pieces, and culturing at the constant temperature of 30-31 DEG C for more than 3 days. The primary culture method disclosed by the invention has the advantages of easy operation, convenient culture, no need of specific cell separation liquid and high culture success rate.

This present invention features methods and composition for the isolation and proliferation of neural crest stem cells (NCSCs) from embryonic tissues as well as from tissues from a post-natal mammal. According to this invention, NCSCs are capable of producing non-neuronal and neuronal cells under the appropriate conditions. The cells of the invention therefore provide an accessible source for autologous and heterologous transplantation into the central nervous system, the peripheral nervous system, as well as other damaged tissues.

The present invention provides a kind of transgenic cotton preparing process. The process includes a bacterially culturing cotton seedling, adopting the hypocotyl piece of the seedling with the growing point retained as the explant, longitudinally cutting the growing point, infecting the explant with agrobacterium carrying exogenous gene, organ differentiating to form test tube plantlet, and grafting transplantation. The process features that the agrobacterium infected cell or tissue is cultured into seedling directly through organ differentiation without forming embryonic tissue. The present invention can shorten the exogenous gene transforming period of cotton obviously, and has no limitation from the genotype of acceptor material, high transforming efficiency, simple operation, low cost and few mutations except that caused by the exogenous gene.

The invention discloses an adjustable flushing-type uterine curettage device for the gynecology department. The device comprises a U-shaped frame main body, the U-shaped frame main body comprises a C-shaped rod at the leftmost end, the upper end and the lower end of the C-shaped rod are bent rightwards to be connected to holding rods, the right end of each holding rod is connected to a fixed rod,the right end of each fixed rod is connected to an arc rod, a scraper rod is connected to the right end of each arc rod, a suction cup is installed at the top of the left side of each scraper rod at the top, an elastic scraper is installed on the outer wall of each scraper rod, a spring column is connected to the left side between two fixed rods, a thumb clamping sleeve is welded to the top of oneholding rod at the top, and sliding rods are slidably installed at the ends, close to each other, of the two holding rods. Through two elastic scrapers arranged in a mirroring mode, the U-shaped frame main body is rotated to drive the two elastic scrapers to rotate, scraping blades are arranged on the two sides of each elastic scraper in the length direction, and embryonic tissue in the uterus can be comprehensively scraped. Scraping columns on the scraping blades scrape mucosal tissues on uterine lining, the uterine lining cannot be damaged, and the safety is good.

The invention relates to a traditional Chinese medicine for clearing embryonic tissues and a preparation method and an application thereof. The traditional Chinese medicine is made from the following raw material medicines in parts by weight: 9-22 parts of ground beeltle, 12-27 parts of leonurus, 4-14 parts of rhizoma sparganii, 4.2-14.5 parts of curcuma zedoary, 6-13.7 parts of peach kernel, 5-16.4 parts of safflower, 1.3-5.8 parts of rheum officinale, 1.2-8 parts of pollen typhae, 6-16 parts of red peony root, 8-24.5 parts of radices trichosanthis, 5-13 parts of corydalis tuber, 5.1-17 parts of radix clematidis, 5-13 parts of talc, 5-15.8 parts of rhizoma alismatis, 5-18.6 parts of angelica and 5-12 parts of radix cyathulae. The traditional Chinese medicine provided by the invention has the function of removing blood stasis, and thus is capable of clearing placenta tissues left in a uterus and promoting the uterus to contract, and the traditional Chinese medicine is singly used or cooperatively used with mifepristone to obviously accelerate the discharge of the fertilized egg, obviously reduce colporrhagia, shorten the bleeding time and obviously improve the clearing rate of embryonic residues in the uterine cavity.

The present disclosure describes mammalian pluripotent embryonic-like stem cells (ELSCs) isolated from corneal limbal tissue, a non-embryonic tissue. The ELSCs of the present disclosure are capable of proliferating in an in vitro culture, maintain the potential to differentiate into cells of endoderm, mesoderm, and ectoderm lineage in culture, and are capable of forming embryoid-like bodies when placed in suspension culture. Thus, these cells possess multi-lineage differentiation potential and self-renewing capability. ELSCs may be a promising therapeutic tool, and may provide new therapeutic alternatives for various diseases, conditions, and injuries.

The invention discloses a method for promoting development and maturation of a Chinese pine somatic embryo (somatic embryo). The method comprises the step of carrying out liquid pretreatment culture on a Chinese pine embryonic tissue, wherein a polyamine synthesis inhibitor regulator is contained in an embryonic tissue pretreatment culture medium. According to the method, the polyamine synthesis inhibitor regulator is added into the liquid pretreatment culture medium and an embryo maturation culture medium, so that purposes of inhibiting excessive proliferation of the embryonic tissue in the embryo maturation process, promoting development and maturation of the somatic embryo and improving the quality and the quantity of the mature somatic embryo are achieved. By using the method, more mature somatic embryos and somatic embryo seedlings can be obtained from a genotype material with weak Chinese pine embryonic nature and excessive proliferation, and diversification of Chinese pine somatic embryo seedling genotypes is facilitated, so that the limitation of clone forestry genotypes can be overcome, and multi-genotype Chinese pine somatic embryo seedlings can be produced in a large-scale and industrialized manner.

The invention discloses a medicine for removing embryonic tissue residues and a preparation method of the medicine. The medicine is prepared from the following raw material medicines: immature bitter orange, carbonized human hair, motherwort herb, Szechwan raspberry, root of Korean raspberry, thlaspi arvense, beautiful sweetgum fruit, descolor cinquefoil herb, cherokee rose fruit, perfoliote knotweed herb, root of sinkiang arnebia, peach seed, Chinese angelica, ginseng, gynostemma pentaphyllum, common ginger, donkey-hide glue and licorice root. Various medicines are combined to ensure that blood stasis can be dispersed, pathogenic heat can be cleared, qi and blood can be replenished, and blood can be returned to meridians, and all medicines can play roles in activating blood and removing blood stasis, clearing heat and stopping bleeding, and nourishing blood and invigorating qi together; and the whole prescription can be used for relieving both primary and secondary symptoms, achieving tonification and purgation in combination, ensuring 'bleeding stopping in blood stasis dispersion', ensuring 'blood stasis dispersion in bleeding stopping', and achieving combined use in circulation and astringing so as to ensure that blood stasis cannot be remained in a bleeding stopping process and blood activation can be achieved without causing massive hemorrhage; and moreover, products with functions of reinforcing qi and nourishing blood are additionally provided so as to ensure that the effects of strengthening the body resistance and removing blood stasis, increasing the tension and motility of the uterine smooth muscles, enhancing the contraction amplitude of the uterine smooth muscles, removing the embryonic tissue residues after medical abortion, reducing the bleeding volume, shortening the vagina bleeding time are achieved, toxic or side effects are small and the safety is good.

The invention discloses a shaping direct-viewing curettage device for induced abortion. The curettage device includes a curettage mechanism, an observation mechanism, a circuit, a negative pressure suction mechanism, an operating rod and a shaping mechanism. The shaping mechanism can adjust the angles and directions of the front end of the operating rod so as to adapt different uterine shapes andpositions. Through the technical scheme of a rear arranged camera, a diagnostic curettage device can be completely in the field of view of the camera, so that full visibility can be realized during operation, and observation dead zones can not exist. Meanwhile, the operation dead zones of the diagnostic curettage device can not exist due to the shielding generated by the height of a lens module; embryonic tissue implanting at any positions in the uterus can be completely removed, especially near the outlet of the fallopian tube which is the implantation position at the bottom of the uterus; and the diagnostic curettage device can also remove the embryonic tissue easily, so that the diagnostic curettage device is safer and more efficient during clinical using. A direct-viewing curettage system for induced abortion is also disclosed. The curettage system including the curettage device can perform operation under the real-time displaying of a display system, so that accurate, safe and efficient abortion operation can be realized.

The invention discloses a human diploid cell ZFB as well as a construction method and a large-scale culture method thereof, a classification name of the cell is ZFB-2, and a preservation number is CGMCC No. 17481; the preservation number of the ZFB-3 is CGMCC No.17482; the construction method of a ZFB cell strain comprises the following steps: taking embryonic tissue blocks for adherent culture; when the cells grow into a compact monolayer, culturing the monolayer cells by adopting an MEM culture solution containing 10% of fetal calf serum, and carrying out passage; discarding a cell culture supernatant, and digesting and cryopreserving; the large-scale culture method of the ZFB cells comprises the following steps: inoculating a ZFB cell strain of a working cell bank into a primary bioreactor for cell amplification; taking a microcarrier covered with cells in the primary bioreactor, washing, adding trypsin containing 0.1% of collagenase, and digesting; and then inoculating into a secondary bioreactor for culturing. The ZFB-2 cell and the ZFB-3 cell provided by the invention are good in state, rapid in growth, long in passage life, high in sensitivity to viruses and convenient to widely use.

The present invention provides methods and compositions for establishing and maintaining growth of cells and embryonic tissue on a synthetic polymer matrix. For example, the present invention provides synthetic growth matrices for stem cells, gametes, mature differentiated cells, and embryonic tissue (e.g., blastomeres, embryos, and embryoid bodies). In certain embodiments, the cells are capable of going through multiple passages while remaining in an undifferentiated state as a result of the synthetic polymer matrix.

The invention discloses a primary culture method and special reagents for cuttlefish embryonic cells. The primary culture method includes the steps of collecting fertilized eggs, obtaining embryonic tissues and cells and performing primary culture of the embryonic cells. The specific reagents for the primary culture specifically include culture medium solution, methylcellulose, D-glucose, calf serum, cuttlefish serum and double antibiotics. The primary culture method and special reagents for cuttlefish embryonic cells have the advantages that the number of separated cells by the primary culture method is large, the disinfection is complete, the damage to an embryo during separation can be reduced, the number of embryonic cells attached to the wall is large, the success rate of the cultureis high, and the survival time is long; the special reagents can stimulate the cells themselves to secrete more growth factors, the division of the embryonic cells is accelerated and the cell culturetime is shortened.

The invention relates to the technical field of tissue embryo teaching, and discloses a low-temperature fresh-keeping storage device for tissue embryo teaching, which comprises a cabinet body, a cabinet door adaptively mounted on the cabinet body, at least one base inserted in the cabinet body, at least two carrier plates arranged on the base along the moving direction of the base, and an action mechanism, the top of the carrier plate is provided with at least one vessel containing groove used for fixing vessels containing embryonic tissues. According to the low-temperature fresh-keeping storage device for tissue embryo teaching, when a vessel needs to be stored, all the butt-joint insertion plates on the base can rotate and lift synchronously through the action mechanism when the base is pulled out of the cabinet opening, so that a carrier plate is inserted, and the pulling-out distance of the base relative to the cabinet opening is shortened; the supporting effect between the track surface of the track groove and the base surface in contact with the track groove is reduced, loss between the track surface and the base surface is reduced, damage and deformation are avoided, follow-up use is guaranteed, time and labor are saved, and convenience and rapidness are achieved.

A direct-view induced abortion uterine curettage device with an electronic protection mechanism disclosed by the invention comprises a uterine curettage mechanism, an observation module, a circuit, a negative pressure suction mechanism, an operating rod and the electronic protection mechanism. The uterine curettage mechanism comprises at least one curettage device which is arranged at the front end of the operating rod. The electronic protection mechanism is arranged outside the observation module and / or the circuit and is used for performing waterproof, gas-proof and insulating protection on electronic components of the observation module and / or the circuit. A direct-view induced abortion uterine curettage system disclosed by the invention comprises the direct-view induced abortion uterine curettage device with the electronic protection mechanism disclosed by the invention; meanwhile, a negative pressure aspirator or a flushing system can be arranged according to needs; during a clinical operation, the implantation position of embryonic tissue can be conveniently found through a camera of a lens module; the embryonic tissue is stripped from a uterus by using the curettage device; and then the stripped embryonic tissue is sucked and discharged out of a body by using the negative pressure suction mechanism. The electronic protection mechanism arranged outside the observation module and the circuit can carry out waterproof, gas-proof and insulating protection on the electronic components of the observation module and the circuit, so that blood or tissue fluid generated in the operation process is effectively prevented from influencing normal work of the electronic components, normal operation of the operation is guaranteed, and the clinical process is relatively safe.

A method for inducing embryonic calluses of large-fruit camellia chekiangoleosa comprises the following steps of (1) selecting tender shoots on newly-drawn branches in the same year as explants, carrying out disinfection treatment, and cutting off old wounds for later use; 2) inoculating the explant subjected to disinfection treatment in the step 1) into an induction culture medium for induction culture for 3-4 weeks to obtain the embryonic calluses; and 3) transferring the embryogenic callus obtained in the step 2) into a proliferation culture medium for proliferation culture, and performingsubculture once every three weeks to obtain a large number of embryogenic tissues. The method for inducing is convenient and simple to operate, can quickly achieve induction and proliferation to obtain the large number of embryogenic calluses, and provides a basis for industrial production of the large-fruit camellia chekiangoleosa.

The invention relates to a trimmed woven label and a preparation method thereof. The preparation method includes the preparation of overlock yarn, the design of the fabric structure, the weaving of the fabric, and the cutting of the fabric to obtain the trimmed woven label, wherein: the preparation of the overlock yarn: the overlock yarn is made of the inner core yarn and the outer yarn. Core-spun and twisted, the outer yarn is a low-melting point hot-melt filament, and the inner core yarn is a low-elasticity yarn; weaving: weaving the embryonic cloth according to the embryonic cloth structure, wherein the woven fabric is The technological parameters are as follows: the weaving temperature is between 110 and 160° C., and the pick is 300 to 700 times per minute. The edge-cut woven label prepared by the preparation method has better softness, is not easy to scatter edges, and is dimensionally stable.