The invention relates to an intelligent instrument for measuring the tissue thickness. The intelligent instrument comprises a button cell type intelligent thickness measuring clip or an external power supply type intelligent thickness measuring clip, a linear incision anastomat or a manual thickness measuring instrument or an automatic thickness measuring instrument, and a staple anvil. The button cell type intelligent thickness measuring clip comprises an outer shell, a pressing key, waterproof soft glass, an integrated circuit board and a bottom cover. The waterproof soft glass is arranged between the pressing key and the integrated circuit board and covers the surface of the pressing key. A button cell, a power module, a display module, a tissue pressure intensity measuring module, a tissue thickness measuring module, a control module and a wireless transmission module are mounted on the back face of the integrated circuit board. An eddy-current transducer and a pressure sensor are mounted on the front face of the integrated circuit board. The button cell type intelligent thickness measuring clip is matched with a wireless transmission device. The intelligent instrument for measuring the tissue thickness has the advantages that the tissue thickness can be measured in a quantitative mode, tissue thickness measurement is accurate, corresponding anastomotic staple cartridges can be selected conveniently, and the success rate of anastomotic surgery is increased.