An athletic brassiere with breast band, having the general form of a brassiere, for use when engaging in vigorous physical exercise or activities, such as running, tennis, and the like. An embodiment comprises an inner liner and outer panel, an internal breast band sleeve, and a breast band slidably retained within the sleeve. The breast band has a fastener enabling the breast band length to be adjusted; the breast band is adjustable vertically and horizontally. Embodiments hold the breasts down with the breast band slidably retained within the garment, providing support and protecting the wearer from discomfort or injury from excessive upward and downward breast movement. The breast band alleviates upward and downward movement of the breasts and does not cinch, flatten or compress the wearer's breasts against the chest wall, nor lift and bold the breasts. The garment is attractive and can be worn alone or under another garment.