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316 results about "Adherent Culture" patented technology

A type of culture in which cells grow as a monolayer that is attached to the culture substrate.

Preparation method of allogenic mesenchymal stem cells by CRISPR (clustered regularly interspaced short palindromic repeats) technique editing and IGF (insulin-like growth factor) optimization and application of allogenic mesenchymal stem cells in treating myocardial infarction

ActiveCN105985985AImprove anti-apoptotic abilityPromote homingUnknown materialsFermentationAntigenInflammatory factors
The invention belongs to the field of allogenic mesenchymal stem cells, and particularly relates to a preparation method of allogenic mesenchymal stem cells by CRISPR (clustered regularly interspaced short palindromic repeats) technique editing and IGF (insulin-like growth factor) optimization and application of the allogenic mesenchymal stem cells in treating myocardial infarction. The preparation method comprises the following steps: carrying out separation by density gradient centrifugation to obtain allogenic single karyocytes, and carrying out adherent culture to obtain mesenchymal stem cells; designing a mesenchymal stem cell surface antigen B2M-gRNA and an inflammatory factor TNF-alpha-gRNA; establishing recombinant slow virus particles, and transfecting the mesenchymal stem cells; optimizing the mesenchymal stem cells by using IGF-1; and preparing drugs for treating myocardial infarctions by using the modified and optimized mesenchymal stem cells. The CRISPR/Cas9 technique is utilized to remove the antigens capable of causing immunological rejection and the inflammatory factors capable of causing inflammatory reaction on the mesenchymal stem cell surface, and the IGF-1 is utilized to enhance the apoptosis resistance of the mesenchymal stem cells and promote the homing of the mesenchymal stem cells, thereby providing a new technical scheme for preparing drugs for treating cardiovascular diseases in clinic. The prepared allogenic mesenchymal stem cells can not cause immunological rejection after cell transplantation.
Owner:SUZHOU UNIV

Half-dry solid-state adherent culture device for microalgae industrial production

The invention belongs to the field of microalgae culture, and in particular relates to a half-dry solid-state adherent culture device for microalgae industrial production. The half-dry solid-state adherent culture device comprises a bracket system, adherent culture units, a supporting shaft transmission system, a speed regulating motor, liquid supplementation pipes, collection grooves and a culture medium liquid storage tank, wherein at least one adherent culture unit is arranged on the bracket system and is connected with the speed regulating motor through the supporting shaft transmission system; the liquid supplementation pipes are respectively arranged above the adherent culture unit and are communicated with the culture medium liquid storage tank through pipelines; the collection grooves are respectively formed below the adherent culture unit and are communicated with the culture medium liquid storage tank through liquid collection pipes; a liquid circulating pump and a carbon supplementation device are arranged in the culture medium liquid storage tank. The half-dry solid-state adherent culture device can be used for producing microalgae biomass and secondary metabolites on a large scale; the yield in unit floor area is effectively improved; the culture period is shortened; the culture water consumption is greatly reduced; the cost of the culture device and the operation cost are reduced; the industrial magnification is facilitated.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Inserting-plate type microalgae semi-dry solid adherent culture device

InactiveCN103289888AReduce water consumptionAchieve ultra-high density cultureSolid phase fermentation bioreactorsEngineeringBottle
The invention belongs to the field of microalgae culture, and particularly relates to an inserting-plate type microalgae semi-dry solid adherent culture device. The inserting-plate type microalgae semi-dry solid adherent culture device is characterized by comprising a supporting truss, culture plates, liquid distributing pipes, liquid collecting tanks and a liquid storage bottle, wherein the supporting truss is provided with at least one culture plate, and one liquid distributing pipe is arranged above each culture plate; the liquid collecting tank arranged on the supporting truss is arranged under each culture plate; all the liquid collecting tanks are communicated with the liquid storage bottle by the main liquid pipe; the bottom of the liquid storage bottle is respectively provided with a circulating pump and a carbon-supplementing gas distributing pipe communicated with a carbon dioxide source, and the circulating pump is communicated with a liquid distributing pipe by a pipeline; and each culture plate is provided with a picking scraper. The inserting-plate type microalgae semi-dry solid adherent culture device has the advantages that the yield in unit occupied area is effectively increased, the culture period is shortened, the water consumption for culture is greatly reduced, the cost and the operating cost of the culture device are reduced and the industrial culture on large scale is easily realized.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Separation method for human amniotic mesenchymal stem cells

The invention provides a separation method for human amniotic mesenchymal stem cells (hAMSCs) in a process of cultivating the hAMSCs through enzyme digestion and epidermal growth factor (EGF). The cultivating process comprises the steps of separation of hAMSCs, primary culture of hAMSCs, hAMSCs subculturing and amplification, hAMSCs cryopreservation and recovery, and hAMSCs immunophenotyping detection. According to the method disclosed by the invention, step-by-step digestion is implemented for multiple times through trypsin and collagenase to collect amnion cells, and adherent culture and digestion time control are combined through adding of 4ng / ml of EGF (Epidermal Growth Factor), so as to obtain human amniotic mesenchymal stem cells (hAMSCs) which are relatively high in both purity and activity. The method disclosed by the invention, which combines enzyme digestion and EGF, has the advantages of being simple to operate, rapid, practical and the like, being an effective method for separating and purifying hAMSCs.
Owner:陆华

Method for separating MVs (microvesicles) and MV exosomes from tumor cell supernatant

The invention discloses a method for separating MVs (microvesicles) and MV exosomes from a tumor cell supernatant. The method comprises the following steps: after adherent culture of tumor cells, a culture solution is taken and subjected to centrifugation, the supernatant is mixed with a coupling compound, the mixture is incubated at 4 DEG C under the 100-300-rpm horizontal vibration condition for 3-16 h, a liquid is removed, an incubated coupling compound is obtained and cleaned with PBS (phosphate buffered saline), and coupling microspheres with MVs adsorbed are obtained. The total MVs extracted with the method retain the structures and components of all the MVs, and the yield is high. Secondary sorting can be performed, specific MVs can be analyzed, and the extraction efficiency and the research value are increased. The total extraction time requires only at least 4 hours to finish purification of the MVs and requires only at least 5 hours to finish purification of the exosomes. The extraction purity is higher, and experiments show that the extracted MVs account for 90% or higher of the total extracted proteins while organic precipitate accounts for lower than 20%.
Owner:ZHEJIANG PROVINCIAL HOSPITAL OF TRADITIONAL CHINESE MEDICINE

Domestication method of full suspension culture type Marc-145 cell line

The invention provides a domestication method of a full suspension culture type Marc-145 cell line. The domestication method comprises the following steps: (1) cultivating adherent-culture Marc-145 cells; (2) domesticating a low-serum adherent-culture Marc-145 cell line; (3) domesticating low-serum full suspension culture type Marc-145 cells; and (4) domesticating serum-free full suspension culture type Marc-145 cells. With the application of the cell domestication method disclosed by the invention, the Marc-145 cells of suspension culture can be obtained; the cells are adaptive to low-serum and serum-free full suspension culture; the density of the cells, when subjected to serum-free culture in a 7.5L reactor, can reach 8*10<6> / ml, and at least 4.4*10<10> cells can be obtained, equal to the number of cells obtained from 80-90 15L roller bottles or 18-20 40-layer cell plants; and the domestication method disclosed by the invention is significant in effect.
Owner:NORTHWEST UNIVERSITY FOR NATIONALITIES

Autologous adipose-derived mesenchymal stem cell culture method and used culture medium

The invention relates to an autologous adipose-derived mesenchymal stem cell culture method and a used culture medium. Specifically, the culture method disclosed by the invention comprises the following steps of culture pretreatment, adherent culture, fluid replacement and fluid change, one time or optional multiple times of cell passage, optionally preparing an adipose-derived mesenchymal stem cell preparation and optionally performing cell cryopreservation and revival on the obtained adipose-derived mesenchymal stem cells. The the culture medium disclosed by the invention comprises DMEM and FBS. When the culture medium disclosed by the invention is utilized to culture the autologous adipose-derived mesenchymal stem cells, especially the autologous adipose-derived mesenchymal stem cells of eye adipose cells, an excellent technological effect of the culture method disclosed by the invention can be presented; the obtained autologous adipose-derived mesenchymal stem cells can be applied to autologous application; for example, the autologous adipose-derived mesenchymal stem cells obtained from eye adipose can be applied to face injection such as injection filling at parts of face wrinkles, temple and lacrimal sulcus.
Owner:广东博雅干细胞科技有限公司

Multipotential stem cell-derived mesoderm pedigree mesenchymal stem cell and preparation method thereof

ActiveCN107937338AIncreased therapeutic functionSolve the problem of limited sourcesCulture processSkeletal/connective tissue cellsCell phenotypeGerm layer
The invention discloses a multipotential stem cell-derived mesoderm pedigree mesenchymal stem cell and an induced differentiation method thereof. The multipotential stem cell-derived mesoderm pedigreemesenchymal stem cell is prepared by the following steps: performing in-vitro adherent culture on multipotential stem cells and maintaining the undifferentiation state of the multipotential stem cells; preparing unicellular or cell mass suspension from the cells, inoculating to a culture dish coated with matrix glue, and performing cultivation by using the multipotential stem cell culture liquid;after the cells adhere to the wall, adding GSK-3 pathway inhibitor combination into the culture liquid; after growing for 2 to 10 days, obtaining a mesoderm progenitor population; and after subculturing continuously for 2 to 6 times by using mesenchymal stem cell culture liquid, detecting the cell phenotype of mesenchyme. The defects that the human-derived mesenchymal stem cells and the mesenchymal stem cells derived from other non-finite induced way multipotential stem cells have heterogeneity and hybridity are overcome, and the obtained mesoderm pedigree mesenchymal stem cells have higher proliferation capability and immunoregulation capability; and the standardized induced differentiation process can guarantee that the cell populations obtained from different batches have high consistency.
Owner:SUN YAT SEN UNIV
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