801 results about "Culture plates" patented technology

A method of determining the presence of soybean sudden death syndrome resistance in the soybean plant in a greenhouse setting, the method comprising the steps of: (a) inoculating soil with a low density inoculum of Fusarium solani; (b) planting a soybean plant in said inoculated soil; (c) growing said plant in said soil in a greenhouse; (d) isolating Fusarium solani-infected tissue from said plant; (e) culturing said infected tissue for a period of time sufficient to allow for fungal colony forming unit growth; (f) scoring at least one of disease severity and infection severity in said plant using the number of said fungal colony forming units; and (g) correlating at least one of said disease severity and said infection severity to at least one of disease severity and infection severity data from genetic markers associated with soybean sudden death syndrome resistance to identify a correlation, wherein a statistically significant correlation indicates presence of soybean sudden death syndrome resistance in said soybean plant. Also provided is a method of characterizing resistance to soybean sudden death syndrome in a soybean plant, the method comprising the steps of: (a) isolating roots from a soybean plant infected by Fusariurn solani; (b) culturing the root on a culture plate including a restrictive growth medium that provides for slow fungal growth and restricted bacterial growth; (c) determining root infection severity by statistically evaluating the number of Fusarium solani colony forming units on said culture plate; and (d) characterizing resistance to soybean sudden death syndrome in said soybean plant based on said determined root infection severity.

The invention discloses a device used for culturing and treating cells or tissues, which comprises a culture plate and a movable cover which can be arranged on the culture plate. The culture plate comprises a bottom, at least one culture hole extending out from the bottom, upright side walls arranged around the culture plate and at least a passage extending outside the culture plate from the internal surface of the bottom. The bottom comprises the internal surface of an upper side and the external surface of a lower side. The cover comprises an upper part and a lower part, a chamber arranged between the upper part and the lower part, at least an inlet conduit for liquid to flow into the chamber, at least an outlet conduit extending out from the lower part and a bounding wall extending from the peripheral hem of the lower part. The outlet conduit is aligned with the culture holes. The invention further discloses a method for culturing and treating cells or tissues. The invention provides the device for dynamically culturing and treating cells based on the multihole culture plate. On one hand, the device facilitates industrial production; on the other hand, the device is applicable for the high-flux dynamic culture and treatment of cells.

A multi-well plate can be loaded with a range of compliant substrates. Commerically-available assays can be used to test cellular responses across a plate with shear modulus from 50 to 51200 Pascals. Cells can be grown in the plates, and can be manipulated and analyzed. Hydrogels can be attached to the bottom of a well. The plates can support the attachment and growth of different cell types and can be compatible with standard 96-well and 384-well plate assays. The mechanical properties of the hydrogels can be reproducible and stable to increase the shelf life of the substrate. The hydrogel can be compatible with growth of a variety of cell types, various attachment ligands such as collagen I, collagen IV, flbronectin, vitronectin, laminin, or RGD peptides and can be coupled to the gel surface.