The invention discloses an amplification method for an NK
cell without trophocyte. The method comprises the following steps: adopting a blending culture solution for culturing the NK
cell and culturing for 7 days, and then using a self-
plasma culture medium for culturing, supplying the fluid per 2-3 days in the culturing process, maintaining the
cell density of 2.0*106 / mL and culturing for more than 20 days. The blending culture solution is composed of 1-10% of self-
plasma, 200-1500 IU / mL IL-2, 10-50ng / mL IL-15 and 10-50ng / mL IL-21 added into a
lymphocyte culture medium. The self-
plasma culture medium is composed of 1-10% of self-plasma and 200 IU / mL IL-2 added into a serum-
free medium. According to the amplification method for the NK cell without trophocyte disclosed by the invention,
cord blood or autologous
peripheral blood is taken as a cell source, no trophocyte is required in the culturing process, the amplification efficiency is high and the purity of the acquired NK cell is high. The NK cell acquired according to the method can be used as a
medicine compound for cell immunization therapy and is mainly used for treating and / or preventing infectious diseases and / or
cancer.