Novel efficient method for separating human T-Lymphocytes by immunomagnetic beads

Abstract

The invention establishes an operation method in T-Lymphocytes culture in vitro by immunomagnetic bead separation technique to maximize the separation of human T-Lymphocytes from the same volume of human peripheral blood to obtain a lot of purified T-Lymphocytes and cover the shortage that lymphocytes can be only primary cultured but not be subcultured to the greatest extent. The method is simple and practicable.

Description

technical field [0001] The invention belongs to the field of cell culture, and in particular relates to the sorting of human T lymphocytes and the operation method of the immunomagnetic bead separation method. Background technique [0002] Immunomagnetic bead sorting is an advanced cell separation technology widely used in scientific research. It combines cell surface antigen molecules with specific monoclonal antibodies connected to magnetic beads, and under the action of an external magnetic field, cells connected to magnetic beads through monoclonal antibodies are attracted and retained in the magnetic field; without this surface antigen Cells, because they cannot bind to the specific monoclonal antibody attached to the magnetic beads, cannot stay in the magnetic field, thereby separating the cells. As cells grown in suspension, T lymphocytes cannot be isolated from adherent cells by adherent selection. Therefore, the method of sorting T lymphocytes can be mostly used i...

AI Technical Summary

Problems solved by technology

The culture of T lymphocytes belongs to the primary culture. Due to the inability to be passaged, the number of living cells used in the experiment gradually decreases with the prolongation of the experiment time.

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