1946 results about "Sputum" patented technology

Apparatus and methods are disclosed by which a closed ventilating system accommodates multiple access to the respiratory system of an intubated medical patient without compromising the closed character of the system. Access to the respiratory system through one or more access sites of the closed system apparatus is provided at proximal adapter ports to ventilate the lungs of the patient with gas or gases, to aspirate secretions from the lungs, to oxygenate the lungs to eliminate or reduce residual co2 therefrom, to visually inspect selected parts of the respiratory system, to sample sputum and gases, to sense parameters such as flow rates, pressure, and temperature, to flush with washing solution, and / or to administer medication, gases, and / or lavage. A distal swivel fitting provides multiple sealing sites by which entry of atmosphere is prevented.

The present invention relates to compositions and methods for preserving and extracting nucleic acids from saliva. The compositions include a chelating agent, a denaturing agent, buffers to maintain the pH of the composition within ranges desirable for DNA and / or RNA. The compositions may also include a reducing agent and / or antimicrobial agent. The invention extends to methods of using the compositions of the invention to preserve and isolate nucleic acids from saliva as well as to containers for the compositions of the invention.

Apparatus and methods are disclosed by which a closed ventilating system accommodates multiple access to the respiratory system of an intubated medical patient without compromising the closed character of the system. Access to the respiratory system through one or more access sites of the closed system apparatus is provided at proximal adapter ports to ventilate the lungs of the patient with gas or gases, to aspirate secretions from the lungs, to oxygenate the lungs to eliminate or reduce residual co.sub.2 therefrom, to visually inspect selected parts of the respiratory system, to sample sputum and gases, to sense parameters such as flow rates, pressure, and temperature, to flush with washing solution, and / or to administer medication, gases, and / or lavage. A distal swivel fitting provides multiple sealing sites by which entry of atmosphere is prevented.

A low-frequency lung vibration apparatus is designed to vibrate lungs clogged by sputum, using acoustic waves. The freed sputum becomes easier to be removed by expectoration. The apparatus includes a garment composed of layers, a plurality of electro-mechanical or dynamic vibrators and a controlling unit. An inner layer contains a loading and shape conforming material, while an outer shell holds the vibrators, the controller and the inner garment together. An outer shell is made of a non-stretch material. Several fasteners / zippers hold the inner garment in place and several strategically placed straps allow a tight fit of the garment. The loading created by the layers of the apparatus is tailored to cause the existence of a uniform compression-expansion resonance of the lungs beneficial for sputum removal. A controlling unit allows the detection of a person's lungs resonance frequency and controls the vibrator(s) frequency and amplitude during a treatment.

In a biological measurement system (10) for collecting one or more samples of sputum and / or mucus from a patient in the form of an aerosol and for analyzing said one or more samples to detect whether or not pathogens are present therein, the one or more samples are in solution within the system (10) and detection of the pathogens is performed using a fluorescently labeled assay. The system (10) is adapted to detect bacterial pathogens using evanescent-wave spectroscopy preferably by using a single-reflective technique. The one or more samples are advantageously provided to the system (10) in aerosol form. However, the system (10) is capable of being adapted for use in analyzing samples in liquid form. Methods of analyzing said one or more samples in the system (10) are described.

The invention provides an officinal health-care solid electronic aerosolization liquid, mainly comprising the following components by weight percent: 35-45% of propylene glycol, 30-35% of medicament, 10-15.0% of deionized water, 3-3.5% of tobacco leaf extract solution, 3-3.5% of tobacco flavour, 2-3.0% of excipient, 0.2-1.0% of calcium pectate and 0.3-0.8% of curing agent. The invention also provides a preparation method of the officinal health-care solid electronic aerosolizatioon liquid. The invention contains multiple officinal components having treating function on lungs, thus having treating function on symptoms such as dryness and itch of the throat and irritable cough which are caused by smoking, chronic pharyngitis and dryness of mouth and nose, thirst and dry cough, less sputum or distressed cough caused by the fact that sputum is glued; meanwhile the invention is solid, thus the stability of storing and use is improved, production and transportation are more convenient and flexible, and fragrance can be remained to be more complete.

The invention relates generally to the fields of making biological unit lysates or admixtures of body fluids and of RNA analysis. More specifically, it relates to direct methods for the detection of a specific sequence of RNA in a biological unit, for example a virus, cell or tissue sample, or a body fluid, for example saliva, sputum, blood plasma, etc. More generally, the invention may be used to enzymatically manipulate and protect the RNA in lysate or bodily fluids for a number of applications.

A method of facilitating the obtaining of a mucus sample from at least one lung of a subject comprises administering to at least one lung of the subject, in an amount effective to hydrate lung mucous secretions and / or stimulate cilia beat frequency therein, uridine 5'-triphosphate, an active analog thereof, or a pharmaceutically acceptable salt of either thereof, and, optionally, concurrently administering to said at least one lung a physiologically acceptable salt in an amount effective to hydrate lung mucus secretions therein. A sputum or mucus sample is then collected from said at least one lung, which sample can then be analyzed for lung disease. Pharmaceutical compositions useful for carrying out the method comprise UTP or a salt thereof, alone or in combination with a physiologically acceptable salt, or a pharmaceutically acceptable salt of either thereof. The composition may be a liquid / liquid suspension composition or a dry powder composition.

The invention relates to a pathological examination, in particular to a liquid-based cell preservation solution used in cytopathological examination of human cervical mucus, sputum, urine, pleural fluid, tracheal mucus and the like. It is characterized in that it is prepared from alcohols, sodium phosphate buffer, edetate disodium, sodium chloride 0.08%-0.12%, potassium chloride, formaldehyde, dithiothreitol, calcium acetate, magnesium acetate, etc. As a result, it can not only maintain the stability of the cell structure. It can reduce the agglomeration and precipitation of cell mucus and the loss of cell rupture, and can also make cells easy to stain, improve the clarity of cell preparation, facilitate the smooth progress of pathological examination, and the cost is low, which is conducive to popularization and use.

A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.

The invention relates to a microfluidic system for processing biological samples comprising a rotary motor; a means for controlling said motor; a platform coupled to the rotary motor and adapted to provide at least one particle-washing structure and one particle receiving structure for receiving washed particles; and a detection zone for detection of particles of the sample in the particle receiving structure while the platform rotates. The invention provides a sample processing system that is both automated and prone to fewer errors than manual processing. This is accomplished using a centrifugal microfluidic platform that can process raw biological samples (e.g., blood, sputum, urine,) in order to perform high-quality bead-based immunofluorescent assays. The invention uses a simple rotary motor and custom-designed plastic disc to perform the sample preparation steps outlined above.

The invention relates to a breathing machine which comprises a control system, an inhalation module and an exhalation module, wherein the exhalation module comprises a switching valve, an exhalation branch, a sputum excretion branch and a pressure regulating valve; the switching valve comprises a first joint, a second joint, and a switching element used for connecting or disconnecting the first joint and the second joint; the first end of the exhalation branch is connected with a patient end, and the second end of the exhalation branch is connected with the first joint of the switching valve; the first end of the sputum excretion branch is connected with the second joint of the switching valve, and the second end of the sputum excretion branch is connected with a negative pressure joint of a negative pressure source; the pressure regulating valve is arranged on the exhalation branch and is used for maintaining gas pressure of the exhalation branch to be in a preset range; and the gas pressure comprises positive pressure P+ and negative pressure P-. Therefore, the breathing machine disclosed by the invention has the advantages that a negative pressure sputum excretion function is increased on the basis of the existing breathing machine, normal human coughing can be simulated, and the problem that a clinical mechanical ventilation patient has sputum excretion difficulty is solved. Moreover, compared with the existing breathing machine and sputum excretion machine combination, the breathing machine disclosed by the invention is simple and compact in structure.

The invention relates to a system and method for intelligently identify and count sputum smear microscopic image tubercle bacillus. The system includes a microscopic imaging device which is additionally provided with a CCD image sensor and the microscopic image of the tubercle bacillus is input into a computer by the CCD image sensor and an image collecting card; the lower side of the microscopic imaging device is provided with a 3D accurate control platform used for finishing the automatic switches of automatic focusing and continuous eyeshot so as to realize dynamic and automatic checking on the continuous eyeshot. A dry lens is adopted as an object lens and a multiple extender lens is added in a light path. Computer control realizes the dynamically intelligent identifying and counting on the tubercle bacillus with continuous eyeshot of the same sputum smear. The invention can replace manual spelling and counting, overcome the defects of manual spelling, improve the efficiency and accuracy of a sputum smear lens detecting method and is simultaneously helpful for improving the scientific level of a normal detecting method of the tubercle bacillus. The invention provides a scientific evidence for the clinical diagnosis and control of tuberculosis.

The present invention relates to the detection of a cell proliferative disorder associated with alterations of microsatellite DNA in a sample. The microsatellite DNA can be contained within any of a variety of samples, such as urine, sputum, bile, stool, cervical tissue, saliva, tears, or cerebral spinal fluid. The invention is a method to detect an allelic imbalance by assaying microsatellite DNA. Allelic imbalance is detected by observing an abnormality in an allele, such as an increase or decrease in microsatellite DNA which is at or corresponds to an allele. An increase can be detected as the appearance of a new allele. In practicing the invention, DNA amplification methods, particularly polymerase chain reactions, are useful for amplifying the DNA. DNA analysis methods can be used to detect such a decrease or increase. The invention is also a method to detect genetic instability of microsatellite DNA. Genetic instability is detected by observing an amplification or deletion of the small, tandem repeat DNA sequences in the microsatellite DNA which is at or corresponds to an allele. The invention is also a kit for practicing these methods.

The invention relates to a device and a method for detecting sputum smear tubercle bacillus quickly. The device comprises a support, a charge coupled device (CCD) image sensor, a microscopic imaging system, a computer system, a three-dimensional accurate control console, an X-axis motor, a Y-axis motor, a Z-axis motor, wherein a high-accuracy grating ruler with a grating sensor is arranged in the Z-axis direction of the support additionally; a light-emitting diode (LED) light source plate of which the light intensity is controllable is positioned on the lower side of the microscopic imaging system and is used as an object stage; and the computer system comprises three software functional modules, namely a control module, an image target fusion module and a neural network classification and counting module. The method comprises a visual sense area interpretation algorithm and an optimum detection path scanning algorithm which are used for image detection and a method for fusing targets twice in image segmentation. By the device and the method, the detection accuracy and efficiency of machine vision can be improved, and the scientific reference is provided for the clinical diagnosis and prevention and treatment of tuberculosis.

The invention discloses an automatic sputum suction type artificial airway. The automatic sputum suction type artificial airway comprises an airway duct, wherein an air ventilating channel is arranged inside the airway duct, and an air inflation bag and an air inflation tube are arranged on the airway duct. The automatic sputum suction type artificial airway is characterized in that a sputum suction tube is arranged in the air ventilating channel, and a sputum suction channel is formed inside the sputum suction tube. A sputum suction system comprises a sputum collecting bottle, a sputum-pumping pump and a control circuit, wherein a sputum inlet tube and a sputum outlet tube are arranged on the sputum collecting bottle, a sputum-pumping inlet tube is arranged on the sputum-pumping pump, the sputum inlet tube is communicated with the sputum suction tube, the sputum-pumping inlet tube is communicated with the sputum outlet tube, and the control circuit controls the sputum-pumping pump to carry out sputum pumping. The automatic sputum suction type artificial airway and the sputum suction system avoid stimulation caused by an existing movable sputum suction tube to the trachea wall of a patient in the process of sputum suction, and damage caused by the existing movable sputum suction tube to trachea mucosae, overcome the defect that hypoxia is caused by removal of a respirator, and relieve pain of the patient. Medical workers only need to carry out analysis on the symptoms and the physical sign of the patient, and set relevant parameters of a device, the device can carry out automatic sputum suction, and workload and working intensity of the medical workers are reduced.

A method for detection of mycobacterium tuberculosis antigens in biological fluids provides immunoassay methods, diagnostic kits, and an immunochromatoraphic assay device for detection of Mycobacterium tuberculosis antigens in biological specimens, preferably body fluids and tissues. The preferred body fluids are blood, serum, plasma, urine, pulmonary fluid, sputum, cerebrospinal fluid, and the preferred tissue is the lung biopsy specimen. The immunoassays require two primary antibodies against RD1, RD2, or RD3 of Mycobacterium tuberculosis. At least one of the primary antibodies is attached to a solid carrier. Optional, a second antibody against an animal species producing one of the primary antibodies can be added. Either the other primary antibody or the secondary antibody is labeled with a detection agent, which can be an enzymatic marker, a fluorescent or luminescent agent, a radio active label or a color particle. The biological specimens may be used directly, concentrated or diluted for the immunoassays.

To provide a tracheostomy tube which can be easily attached to and detached from an incision site and in which sputum or the like does not readily longer, a tracheostomy tube A includes a lumen body 10 having an airway securing lumen 10b, a connector section 11 formed at the base end portion of the lumen body 10, and a cuff 13 which is formed on the outer circumference of the tip end portion of the lumen body 10 and which can be expanded and contracted. A circumferential wall 10a of the lumen body 10 defines a cuff-expanding lumen 15 which puts the surface portion of the connector section 11 in communication with the inside of the cuff 13. it also defines a suction lumen 14 allowing the surface portion of the connector section 11 to communicate with the surface portion of the lumen body 10. A coating layer exhibiting surface lubricity in moisture is formed on the surface of the tracheostomy tube A and the inner surface of the lumen body 10 in which the airway securing lumen 10b is formed.

The invention belongs to the technical field of microorganism molecular biological detection, and specifically discloses a host removal extraction method for a sputum microorganism metagenome. The method comprises primary sputum liquefaction, secondary sputum liquefaction, microorganism wall breaking, DNA extraction and nucleic acid quality detection. The invention further discloses a database building method for a sputum microorganism metagenome, wherein the method comprises genome DNA extraction, genome DNA treatment, library amplification, library pre-purification, two-step fragment selection and computer detection. A mild digestion method combining trypsin with SDS is adopted to release a host genome to a solution, thereby obtaining microorganism with complete cell wall structure maintained and extracting nucleic acid from microorganisms with host removed. The provided extraction method and database building method are good in compatibility, and can be widely used to a mainstream sequencing platform. The methods are flexible and simple, efficient and fast, and cost-saving, and have bright popularization application prospects.

A bagged lip sunning tobacco and a preparation method thereof are characterized in that the tobacco powder particles sacked are included. The tobacco powder particles are mostly comprised by the following ingredients which are 30 percent to 55 percent sunning tobacco, 35 percent to 60 percent water, 0.5 percent to 6 percent edible salt, 1 percent to 3.5 percent sodium bicarbonate, 0.02 percent to 8 percent spices and 0.5 percent to 8 percent humectants. The preparation method of the tobacco powder particles is that the sunning tobacco is mixed after comminution and screen separation and the water and the edible salty are added in, after adopting water vapor fumigation or ferment, the spices and the humectants are added and mixed evenly. The method has the advantages that the harassment that the traditional tobacco brings smoke is conquered and the method can provide the contented sensation similar to the traditional cigarette products to the tobacco consumers to reduce the damage to health because of smoking. The invention takes the sunning tobacco which is unique in the country as the ingredients to adopt the taste character of the domestic consumers. The method is convenient to use, by which, not only passive smoking is avoided but also the lip people can get the efficacies of promoting salivation and exterminating sputum.

The invention discloses an e-cigarette oil with the effect of clearing the lungs and moistening the throat. The e-cigarette oil includes a base liquid and a traditional Chinese medicine extract, the base liquid includes propylene glycol and glycerin, and the Chinese medicine extract used Chinese medicinal materials are selected from one or more of Luo Han Guo, bellflower, lily, fat sea, mint, fritillaria, tangerine peel, honey, licorice, honeysuckle, reed root, and green fruit. Phlegm and other health effects.

The invention discloses an intelligent total thoracic high-frequency oscillating sputum ejection machine and a sputum ejection method. The sputum ejection machine comprises a hose, a pressure sensor, a conducting wire, a power supply device, a box body and a cover plate, and is characterized by also comprising a pressure jacket, an air oscillator and a cough sensor, wherein the pressure jacket consists of a metal spring ring, a transverse membrane, a longitudinal membrane, skin and an adhesive buckle; and the metal spring ring is arranged at the bottom of the skin, the transverse membrane is arranged above the metal spring ring, the longitudinal membrane is arranged inside the skin, the upper end of the longitudinal membrane is connected with the upper edge of the inner wall of the skin, and the lower end of the longitudinal membrane is vertical to the transverse membrane and is reserved with a gap. In order to solve a new problem that fixed two-point type anterior chest wall pulse cannot beat the lateral surface and the back of the chest so as to cause poor sputum ejection effect for partial patients, the sputum ejection machine and the sputum ejection method adopt intelligent air supply modes of an electric motor and an air pressure compensator to periodically supply pulse airflow to the pressure jacket, can adjust the pulse airflow according to different press degrees required by patients, and are greatly convenient to treat the patients of different types.

The invention discloses an oxygen supply and sputum suction visual device for the breath internal medicine department. The oxygen supply and sputum suction visual device comprises a breathing mask, a sputum suction device, an oxygen inhaler and a visual controller, wherein the breathing mask is composed of a mask support main body, a mask attaching ring, a face protecting jacket and a mask fixing belt; the sputum suction device is composed of a sputum suction pipe and a cleaning pipe; the oxygen inhaler is composed of a humidifier, an oxygen tank, an oxygen inhaling tube and an air outlet; the visual controller is composed of a display screen and a control button, the display screen is connected with a speculum, and a magnifying lens is arranged between the display screen and the speculum. The oxygen supply and sputum suction visual device is simple in structure and convenient to use, the real-time monitoring can be realized, and thus very good economic benefits are obtained.

It is intended to provide: a sampling device for quantitatively sampling a liquid sample having a high viscosity such as sputum; an auxiliary jig therefor; an agent for homogenizing sputum by which sputum can be quickly treated under mild conditions and a microbe contained in the sputum can be detected at an improved stability; a homogenization device; a homogenization method; a method of detecting a microbe by using the above method; a separation device for conveniently, quickly and efficiently collecting a microbe contained in sputum; a method of collecting a microbe; and a method of detecting a microbe by using the above method. A sampling device for a viscous sample includes: a tubular body; a gasket; a long rod-like plunger; a plate-shaped nozzle that has substantially the same diameter as that of a straight section of the tubular body and is adapted to close a distal end of the tubular body; and a slit formed in the plate-like nozzle, the slit having a predetermined shape adapted to serve as an aspiration and discharge port for aspirating and discharging the viscous sample.

The invention relates to a method of producing honey tea extract, which is characterized in that: extracting effective active ingredients from honey, tea, ginger, dried orange peel and others in a certain ratio, according to the corresponding process, preparing into extract. It has the following functions: relieving cough, eliminating sputum and transforming qi, clearing away summerheat, solving constipation, relieving discomfort due to excessive smoking and drinking. It is suitable and convenient for men, women, old and young.

The present invention relates to pharmaceutical kits and methods to treat lung inflammation and redox imbalance in human cystic fibrosis patients using pharmaceutical compositions containing N-acetylcysteine (NAC), pharmaceutically acceptable salts of N-acetylcysteine, or N-acetylcysteine derivatives. In phase I studies, treatment with oral NAC at a dose of from about 1800 mg / day to about 3000 mg / day for a period of 4 weeks produced significant positive effects, namely, it decreased absolute numbers of white blood cells and neutrophils in the sputum and produced concomitant decreases in sputum neutrophil elastase specific activity and sputum interleukin-8 levels, suggesting an amelioration of lung inflammation in the patients. These effects were associated with an increased total GSH level in whole blood as well increased staining for reduced GSH in blood neutrophils, both of which reflect an amelioration of the redox imbalance in the patients. In ongoing phase II studies, oral NAC at a dose of about 2700 mg / day administered in double-blind manner for 12 weeks showed excellent safety and significantly decreased white blood cells in sputum as compared to placebo.

The invention relates to a total bile acid extract of bear bile powder and a preparation method and application of injection thereof. The total bile acid extract of the bear bile powder is prepared by the following steps of: performing reflux extraction on the bear bile powder by using ethanol; recovering the ethanol from extracting solution, and concentrating the extracting solution; and performing alkaline hydrolysis, neutralization, acidification, ethyl acetate extraction, ethyl acetate crystallization and ethanol crystallization. The invention also provides a method for preparing the injection from the total bile acid extract of the bear bile powder. The prepared total bile acid extract of the bear bile powder and the injection thereof can be used for preparing medicaments for resisting bacteria and viruses, relieving cough and reducing sputum and protecting liver and gallbladder. The invention can improve the extraction yield and purity of ursodesoxycholic acid in the total bile acid extract of the bear bile powder and reduce the chenodeoxycholic acid content simultaneously, so the ursodesoxycholic acid content in the total bile acid extract of the bear bile powder is no less than 70 percent and the chenodeoxycholic acid content is no more than 20 percent. The ursodesoxycholic acid content in the total bile acid extract of the bear bile powder is higher, and the ursodesoxycholic acid yield is high and is over 18 percent generally.

The invention relates to a traditional Chinese medicine formula beneficial to relieving cough and reducing sputum and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine formula beneficial to relieving cough and reducing sputum comprises the following raw materials in parts by weight: 10-20 parts of cyclobalanopsis hui, 10-20 parts of longan bacterial parasite, 10-16 parts of phyllanthus emblica fruit, 6-12 parts of Chinese eriosema root, 8-14 parts of humulus scandens, 5-12 parts of beautiful millettia root, 8-15 parts of lawn pennywort herb, 5-10 parts of pitcher plant, 5-10 parts of loquat leaf, 5-9 parts of lemon leaf, 6-12 parts of purslane, 6-10 parts of acronychia pedunculata, 5-11 parts of Chinese chestnut, 4-9 parts of canarium album root, 5-10 parts of solanum pseudocapsicum, 5-10 parts of pink reineckea herb, 5-10 parts of roxburgh sumac and 6-9 parts of phoenix tree leaf. The traditional Chinese medicine formula beneficial to relieving cough and reducing sputum, disclosed by the invention, is obvious in treatment effect and short in treatment course and has the advantages that pneumonia can be exactly and effectively treated; the effects of relieving cough and reducing sputum are realized; and patients can free from the pain of diseases and return to normal work and life as soon as possible.