A liquid-based cell preservation solution

Abstract

The invention relates to a pathological examination, in particular to a liquid-based cell preservation solution used in cytopathological examination of human cervical mucus, sputum, urine, pleural fluid, tracheal mucus and the like. It is characterized in that it is prepared from alcohols, sodium phosphate buffer, edetate disodium, sodium chloride 0.08%-0.12%, potassium chloride, formaldehyde, dithiothreitol, calcium acetate, magnesium acetate, etc. As a result, it can not only maintain the stability of the cell structure. It can reduce the agglomeration and precipitation of cell mucus and the loss of cell rupture, and can also make cells easy to stain, improve the clarity of cell preparation, facilitate the smooth progress of pathological examination, and the cost is low, which is conducive to popularization and use.

Description

technical field [0001] The invention relates to a pathological examination, in particular to a liquid-based cell preservation solution used in cytopathological examination of human cervical mucus, sputum, urine, pleural fluid, tracheal mucus and the like. Background technique [0002] The existing cell preservation solution does not take long to fix the cells and maintain the stability of the cell structure. The cells are easy to deteriorate after more than three days at room temperature, and 50% methanol is added with formaldehyde as a fixed preservative, which is easy to cause cells to form agglomerates and protein Mucus is also easy to agglomerate and deposit, which is not conducive to the dispersion and observation of cells. Neither can solve the problem that there are a large number of red blood cells and mucus in the specimen, because these components will directly affect the cell volume after the cells are made on the glass slide, resulting in the loss of the cell vol...

AI Technical Summary

Problems solved by technology

[0002] The existing cell preservation solution does not take long to fix the cells and maintain the stability of the cell structure. The cells are easy to deteriorate after more than three days at room temperature, and 50% methanol is added with formaldehyde as a fixed preservative, which is easy to cause cells to form agglomerates and protein Mucus is also easy to agglomerate and deposit, which is not conducive to the dispersion and observation of cells

Neither can solve the problem of a large number of red blood cells and mucus in the specimen, because these components will directly affect the cell volume after the cells are placed on the glass slide, resulting in the loss of cell volume

In addition, the general preservation solution lacks inorganic salt buffer solution, and the pH value is unstable, which can easily cause cell rupture and loss of some components, and also cause instability of cytological staining.