Liquid-based cell preservation solution

Abstract

The present invention relates to pathological examination, especially to a liquid-based cell preservation solution used in pathologically examining cells such as human cervical mucus, sputum, urine, thoracic liquid, and tracheal mucus. The liquid-based cell preservation solution is characterized by being prepared from alcohols, a sodium phosphate buffer solution, disodium ethylenediamine tetraacetate, 0.08-0.12% sodium chloride, potassium chloride, formaldehyde, dithiothreitol, calcium acetate, magnesium acetate and the like. The cell preservation solution cannot only maintain the cell structure stability and reduce the agglomerated precipitation of the cell mucus and the cell breakage loss, but also enables the cells easily to be stained, improves the clarity of the cell sheets, and facilitates smooth performance of the examination work. Moreover, the cell preservation solution is low in cost and easy to promote and use.

Description

technical field [0001] The invention relates to a pathological examination, in particular to a liquid-based cell preservation solution used in cytopathological examination of human cervical mucus, sputum, urine, pleural fluid, tracheal mucus and the like. Background technique [0002] The existing cell preservation solution does not take long to fix the cells and maintain the stability of the cell structure. The cells are easy to deteriorate after more than three days at room temperature, and 50% methanol is added with formaldehyde as a fixed preservative, which is easy to cause cells to form agglomerates and protein Mucus is also easy to agglomerate and precipitate, which is not conducive to the dispersion and observation of cells. Neither can solve the problem that there are a large number of red blood cells and mucus in the specimen, because these components will directly affect the cell volume after the cells are made on the glass slide, resulting in the loss of the cell...

AI Technical Summary

Problems solved by technology

[0002] The existing cell preservation solution does not take long to fix the cells and maintain the stability of the cell structure. The cells are easy to deteriorate after more than three days at room temperature, and 50% methanol is added with formaldehyde as a fixed preservative, which is easy to cause cells to form agglomerates and protein Mucus is also easy to agglomerate and precipitate, which is not conducive to the dispersion and observation of cells

Neither can solve the problem of a large number of red blood cells and mucus in the specimen, because these components will directly affect the cell volume after the cells are placed on the glass slide, resulting in the loss of cell volume

In addition, the general preservation solution lacks inorganic salt buffer solution, and the pH value is unstable, which can easily cause cell rupture and loss of some components, and also cause instability of cytological staining.