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Method for fast flaking paraffin by microwave radiation water bath diathermy and device

A microwave radiation and rapid technology, applied in the field of rapid paraffin preparation of biological tissue specimens, can solve the problems of insufficient, too brittle tissue, artificial ice crystals, etc. Membrane and nucleolus clear effects

Inactive Publication Date: 2012-11-07
CHANGSHA COMBINE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the frozen section technique is still the first choice, it often has disadvantages such as too thick slices, blurred tissue cell morphology, ice crystal artifacts, and sliced ​​specimens cannot be preserved for a long time.
In addition, the traditional rapid paraffin section method related to non-frozen section technology mainly uses alcohol lamps, electric furnaces or water baths to directly heat the tissue to shorten the time for tissue fixation, dehydration, transparency and wax immersion; but the disadvantages of this method are The volume of tissue samples is required to be too small, and the time and degree of heating are difficult to control accurately, which often causes the tissue to be too brittle or insufficiently processed, thus affecting the diagnosis
Although the ultrasonic rapid tissue dehydration device or tissue processor (Chinese patent application number: 02153488.8) commercially available on the market can meet the basic application requirements of rapid paraffin sectioning, but because it can only process 1-3 specimens at a time, the work efficiency is low. High, still use traditional poisonous and dangerous reagents such as xylene and acetone
If you want to buy a professional microwave rapid biological tissue processor (Chinese patent application number: 201120067102.7), not only the price of the instrument is quite expensive, but also the consumption of reagents is very large, which is not economical and practical for ordinary users

Method used

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  • Method for fast flaking paraffin by microwave radiation water bath diathermy and device
  • Method for fast flaking paraffin by microwave radiation water bath diathermy and device
  • Method for fast flaking paraffin by microwave radiation water bath diathermy and device

Examples

Experimental program
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Effect test

Embodiment 1

[0021] combine figure 1 , figure 2 with image 3 . figure 1 A schematic diagram of a common commercial microwave oven used as a microwave irradiation tool. Including the microwave oven shell 1, the oven cavity 2, and the oven door 3, place the water container 4 with prepared specimens and reagents in the center of the bottom of the oven cavity 2, and place the tissue sample box 6 and the reagent tube 5 in the reagent tube 5 filled with reagents. Part of the reagent is immersed in the water of the water container 4, adjusted to the central position, the furnace door 3 is closed, the fire gear is set up, and the operation is started.

[0022] figure 2 It is a schematic diagram of the composition of the water bath diathermy part of the rapid paraffin slicing device. Put 500 milliliters of water into a water container 4 with a volume of 1000 milliliters; at the same time, pour 30 milliliters of reagents such as fast fixative solution in the reagent tube 5 and add a cover,...

Embodiment 2

[0025] 1) Materials: Prepare rapid fixative solution (10% formaldehyde solution l5ml, 95% ethanol 80ml and glacial acetic acid 5ml); prepare rapid dehydration transparent agent: (100% isopropanol 500ml, absolute alcohol 400ml, poly Ethylene glycol (molecular weight 300) l00 ml and dimethyl sulfoxide (DMSO) l ml; melting point 58-60 ° C paraffin. Instrument: WP750 Galanz household microwave oven. Pour 100 ml of melted paraffin into a beaker and place it in an electric thermostat Stand by at any time, and the fast paraffin film production device is installed according to the above-mentioned embodiment 1 figure 1 microwave and figure 2 Water bath diathermy components and image 3 Vacuum negative pressure wax impregnated parts.

[0026] 2) Operation steps: (1) Preheating: Add about 500ml of water to a 1000ml beaker, put it in a microwave oven and preheat the water to 60°C for 3-5 minutes on high heat. (2) Fast tissue fixation: put the taken tissue block into a tissue specimen...

Embodiment 3

[0028] 1) Materials: Prepare a rapid decalcification solution: add 16 ml of hydrochloric acid, 10 ml of acetic acid, 20 g of salicylic acid and 174 ml of distilled water in sequence and mix, and the salicylic acid in the solution shows insoluble precipitation. The same equipment as in Example 1 was used.

[0029]2) Operation steps: take the sample to be decalcified with a volume of (1-1.5) cm × (0.7-1) cm × (0.3-0.5) cm and place it in a tissue specimen box. Pour 200 mL of rapid decalcification solution into a 500 mL glass beaker and put the tissue specimen box into it. Take another 1000ml beaker, add 500ml of water, fix the small beaker in the water of the large beaker, then place the two matching beakers in the center of the microwave oven cavity, heat for 5 minutes at high heat level 10, and then use low heat level 1 or 2 Continue to heat for 30 minutes. Use the needle to easily penetrate to judge the decalcification, and repeat the operation if necessary.

[0030] The p...

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Abstract

The invention provides a method for fast flaking paraffin for a biological tissue sample and a device. A microwave radiation water bath diathermy is applied to help reagents which are weak in absorbing energy and paraffin be fast heated. The device for fast flaking paraffin is characterized by comprising a microwave radiation water bath diathermy part having the function of fast processing a tissue sample, and a vacuum negative pressure waxing part having the function of fast waxing the tissue sample. The procedure of flaking paraffin comprises the steps of fast tissue fixing, fast tissue dehydration and transparent handling once or twice and fast waxing. The reagents special for flaking paraffin comprises a fast fixing solution, a fast dehydration transparent reagent and a fast decalcification solution. The method and the device have the flexibility of processing tissue samples individually or in batch and the capability of processing different tissue samples simultaneously, and are stable and reliable in the flaking quality, and applied to the fast paraffin flaking and pathologic diagnosis for biological tissue samples for the pathology and inspection in the hospital, scientific research institutes and other organizations.

Description

technical field [0001] The invention relates to a method and device for quick paraffin preparation of biological tissue specimens, which are applied in departments of hospital pathology, inspection and scientific research to carry out rapid paraffin preparation and pathological diagnosis of biological tissue specimens. Background technique [0002] At present, frozen section technology and other rapid diagnostic techniques are widely used at home and abroad to solve the problem of pathological diagnosis in surgery. Although the frozen section technique is still the first choice, it often has disadvantages such as too thick slices, blurred tissue cell morphology, ice crystal artifacts, and sliced ​​specimens cannot be preserved for a long time. In addition, the traditional rapid paraffin section method related to non-frozen section technology mainly uses alcohol lamps, electric furnaces or water baths to directly heat the tissue to shorten the time for tissue fixation, dehydr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/44
Inventor 钱翔钱津悦钱正泽
Owner CHANGSHA COMBINE MEDICAL TECH
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