48 results about "Tissue Processor" patented technology

A method of managing resources of a histological tissue processor, the tissue processor comprising at least one retort (12, 14) selectively connected for fluid communication to at least one of a plurality of reagent resources (26) by a valve mechanism (40), the method comprising the step of: nominating resources according to one of: group, where a group nomination corresponds to a resource's function; type, where a type nomination corresponds to one or more attributes of a resource within a group; station, where a station nomination corresponds to a point of supply of a resource.

A tissue processor for processing tissue samples for histological analysis. The processor comprises two retorts, wax baths, reagent containers, a pump and valve. The vale distributes the reagent from one container to either retort. Separate reagent lines connect the wax baths to the retorts. A method of infiltrating a sample containing a reagent such as a dehydrating reagent like an alcohol, where the infiltrating material is heated to a temperature at or above the boiling point of the reagent, to boil off the reagent when the tissue sample is contacted by the infiltrating material. The pressure in the retort may be reduced to lower the boiling point of the reagent.

An automatic tissue processor for processing tissue samples for histological analysis. The tissue processor in one configuration includes a retort chamber, a wax storage chamber holding multiple wax containers, a reagent storage chamber holding multiple reagent containers, and a fluid transporting system including a rotary valve operated by a Maltese Cross gear, for selectively connecting the retort chamber with a particular wax or reagent container to supply wax or reagents to the retort chamber. Differential heating of the wax chamber is accommodated by multiple heating elements, with indirect heating of the rotary valve and the fluid transporting system. A computerized central control system for automatic monitoring and control of such tissue processor is advantageously arranged to execute a reagent management program, which enables the central control system to automatically manage reagent usage and replacement.

A tissue processing system includes a tissue processor for processing tissue using reagent fluids, a fluid container and a coupler providing bi-direction fluid communication between the tissue processor and the fluid container. The coupler includes first and second cylindrical rings separated by a wall and a fluid conduit disposed within the first and second cylindrical rings that pass through the wall, thereby providing fluid communication from the fluid container to the tissue processor. Fluid is returned from the tissue processor to the fluid container via at least one fluid return aperture in the wall that separates the first and second cylindrical rings.

A multispectral medical imaging device includes illumination devices arranged to illuminate target tissue. The illumination devices emit light of different near-infrared wavelength bands. The device further includes an objective lens, a near-infrared image sensor positioned to capture image frames reflected from the target tissue, and a visible-light image sensor positioned to capture image frames reflected from the target tissue. A processor is configured to modulate near-infrared light output of the plurality of illumination devices to illuminate the target tissue. The processor is further configured to determine reflectance intensities from the image frames captured by the near-infrared image sensor and to generate a dynamic tissue oxygen saturation map of the target tissue using the reflectance intensities. The device further includes an output device connected to the processor for displaying the dynamic tissue oxygen saturation map.

The invention provides a method for separating and culturing mouse cells, comprising the following steps: (1) disinfecting tissue processing instruments; (2) selecting male BALB/c mice aged 1 to 2 months, killing them by dislocation of the neck, and using surgical scissors Separate the synovial layer and fibrous layer of the joint capsule, then take out the synovial layer tissue, put the tissue into pre-cooled sterile double-antibody-containing PBS solution and wash several times to remove blood stains and fat tissue; (3) Cut the tissue into pieces and use Digest with preheated type II collagenase and trypsin respectively; (4) terminate the digestion, centrifuge, and discard the supernatant; (5) inoculate into the coated culture flask by artificial adherence method; (6) after incubation for 1-2 hours, Cultured in an environment of 37°C and 5% CO2; (7) observation of cell morphology; (8) subculture of cells; (9) identification of cells. The method for isolating and culturing mouse synoviocytes provided by the invention is simple to operate, has a large number of cells and a high survival rate, and is an ideal primary method for isolating and culturing mouse synoviocytes, providing a reliable basis for experiments. cellular resources.

A sensor module (38, 40, 42) for a tissue processor (20) has a thermal sensor (66) for measuring a temperature of a container (30, 32, 34) of the tissue processor (20), and further has a position sensor for detecting a working position of the container (30, 32, 34).

The invention provides a separating and culturing method for olfactory ensheathing cells of rats. The separating and culturing method comprises the following steps: (1) disinfecting a tissue processing instrument; (2) selecting male SD rats of 2-3 months old, killing the male SD rats after anesthesia, separating the olfactory mucosa tissue by adopting surgical scissors, placing the tissue in PBSsolution which is precooled, is sterile and contains double antibodies, and washing the tissue for a plurality of times, so that blood stains and outer membranes are removed; (3) cutting up the tissue, and carrying out digestion by adopting preheated neutral enzyme and trypsin respectively; (4) terminating the digestion, carrying out centrifugation, and discarding the supernatant; (5) inoculatingthe obtained product into an enveloped culture flask by adopting an artificial wall sticking method; (6) carrying out incubation for 1-2 h, and carrying out culturing in the environment being at 37 DEG C containing 5% CO2; (7) carrying out cell purification; (8) carrying out cell morphological observation; (9) carrying out cell subculturing; (10) carrying out cell counting and survival rate testing; and (11) carrying out immunological identification. For the separating and culturing method for olfactory ensheathing cells of rats, the operation is simple, a large number of cells are obtained, the survival rate is high, the method belong to an ideal primary separating and culturing method for the olfactory ensheathing cells of rats, and a reliable cell resource is provided for the experiment.

A tissue processor used for processing tissue sample for histopathology analysis, comprising two retorts, a paraffin tank, a reactant vessel, a bump and a valve from a vessel to any retort to distribute the reactant; a divided reactant tube is connected to the paraffin tank to the retort; a method of permeating a sample comprises a reactant like alcohol; wherein the temperature of the permeation material is heated to equal to or higher than the boiling point of the reactant, so that the reactant can be removed through boiling when using the permeation material in contact with the tissue sample, which can reduce the pressure in the retort so as to reduce the boiling point of the reactant.

The embodiment of the invention discloses a device, a method and a tissue treatment machine for prompting the state of a solvent in a solvent bottle in a tissue processor. The device comprises a detector, a controller and a prompter. The detector is used for detecting the state of the solvent. The controller is used for generating a control signal according to the state. The prompter is used for generating a signal for prompting the state of the solvent according to the control signal. According to the embodiment of the invention, an operator can be visually reminded of the state of the solvent through the signal for prompting the state of the solvent, so that the operator can accurately replace the solvent in time, and solvent replacement errors are avoided.