414 results about "Gentamicin" patented technology

This invention refers to a matter composition of the type used in the treatment of ophthalmic ailments and specifically refers to an ophthalmic carrier solution based on surface-active, emulsifying, antibacterial, antioxidant, etc. Agents which form a carrier that enwraps or masks an active ingredient such as sodium dyclophenac or other antibiotic agents such as tobracin, gentamicin or timolol sulfate, with the aim of avoiding the problems caused by the topical application of the mentioned active ingredients, such as pain, a burning sensation, irritation and other annoyances for the user.

The invention relates to a herbal medicine preparation method for the children bronchopneumonia treatment, belonging to the technical field of Chinese herbal medicine preparation methods; which adopts the technical proposal that: herbs like ginkgo seed, ephedra, apricot kernel, perilla, pinellia ternate, Swallowwort Rhizome (SRh), aster, Tussilago farfara, cortex mori, Chinese wolfberry root-bark, scutelarria, blackberry lily, dyers woad leaf, isatis root, bupleurum, dandelion, honeysuckle, houttuynia cordata, sargentgloryvine stem, sophora tonkinensis, APN (Andrographis Paniculta), Franchet Groundcherry Fruit, balloonflower and liquorice are immersed in 1000ml water for half an hour, and then decocted on mild fire; after decoctation, the herbal liquor is filtered to get 300ml herbal medicine for the treatment of children bronchopneumonia. In prior art, penicillin, streptomycin, and gentamicin are used for the treatment of children bronchopneumonia; the application of penicillin can cause allergic reaction, as a result, the life of the patient may be in danger if rescue is not timely. The preparation method has the advantages of simple manufacturing technology, small side effect and durable and stable therapeutic effect.

The invention relates to a culture medium for a normal epithelial cell of a human or mammal, primary separation culture, subculture, 3D gas-liquid culture and 3D matrigel culture methods, the normal epithelial cell generated by using the culture medium and the culture methods and application of the normal epithelial cell to a toxicological evaluation system. The culture medium is prepared by mixing DMEM and Ham's F-12NUTRIENT MIX according to the volume ratio of 3:1 and also adding 4-6% of fetal calf serum, 1-3nM triiodothyronine, 0.4-0.65% of insulin-transferrin-selenium reagent, 4-6mu g / ml transferrin, 9-11ng / mL epidermal growth factors, 0.3-0.5mu g / mL hydrocortisone, 0.5-1.5nM cholera toxin, 0.4-0.6mu g / mL amphoterrible B, 35-45mu g / mL gentamicin, 45-55nM calpeptin, 35-45ng / ml recombinant human IL-1RA and 3mu g / ml recombinant human R-Spondin-1. The culture medium disclosed by the invention can be used for carrying out separation culture or subculture on the normal epithelial cell of the human or the mammal and any other various tissue source, rapidly proliferating the normal epithelial cell in vitro and establishing a cell line; and the normal epithelial cell is a normal diploid cell and is applied to the toxicological evaluation system of the human or the mammal.

The present invention is directed to a pharmaceutical composition comprising a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. A suitable antibiotic is selected from the group consisting of doxycycline, gentamicin, tobramicin, ciprofloxacin, clindamycin, clarithromycin, azithromycin and metronidazole. Preferred antibiotics are doxycycline and ciprofloxacin. More preferably, the antibiotic is doxycycline. In its second aspect, the present invention is directed to a method for treating periodontal disease in a mammalian patient comprising administering to a mammalian patient in need of such treatment a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. Typically, the mammalian patient is a human.

The invention discloses a treatment method of antibiotic fermenting bacterial residues. The treatment method comprises the following steps: uniformly mixing the fermenting bacterial residues of lincomycin hydrochloride and gentamicin with water, and preparing a suspension; performing anaerobic fermentation treatment on the suspension. The treatment method is an efficient fermentation treatment process of the fermenting bacterial residues of lincomycin hydrochloride and gentamicin and belongs to the technical field of resource utilization of the bacterial residues. By adopting the method disclosed by the invention, the toxicity, the harm and an inhibition effect of residual antibiotics to fermenting bacteria in an anaerobic fermentation process of the traditional antibiotic bacterial residues are overcome, the problems of blockage, short flow and the like which are produced when a traditional filler is fed are simultaneously avoided, the biomass of a hydrolysis acidification pool and an L-fermentation tank is ensured, the gas production is improved and the method further has the advantages of low treatment cost, easiness in control of operation process, high load of a reactor matched with the method, small occupied area and the like.

The invention discloses a preparation method for a silver-containing carbon dot and application of the silver-containing carbon dot to preparation of an antibacterial agent. The preparation method comprises the following steps: with polyethyleneimine (PEI), citric acid and silver nitrate as raw materials, synthesizing a silver-doped functionalized carbon dot solution (a PEI-Ag carbon dot solution)by using a hydrothermal process or microwave process; and then carrying out centrifugation and drying so as to obtain the solid PEI-Ag carbon dot. The preparation method provided by the invention isenvironment-friendly in preparation and uses cheap and easily available raw materials; and the prepared PEI-Ag carbon dot has good biocompatibility and high quantum yield, can be individually used orcooperatively used with antibacterial agents like tetracycline, gentamycin and cefoxitin, and show inhibitory effect on Staphylococcus aureus, Escherichia coli, Candida albicans and the like.

The invention provides a human blood fat (serum / plasma) quality management reference kit. The kit takes human serum (plasma) as a matrix; a human blood fat component (human apolipoprotein A1 antigen, apolipoprotein B antigen, high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL) and the like), a stable system and an anticorrosion system are added to the matrix; the stable system is any combination of a surfactant of Triton series, Tween series, EDTA (ethylene diamine tetraacetic acid) series and the like, a polymer of polyvinyl alcohol series, polyvinylpyrrolidone series, polyethylene glycol series and the like, and a phosphate buffer solution; and the anticorrosion system is any combination of sodium azide, gentamicin, amphomycin and ProClin series. The invention further provides a preparation method of the kit. The kit is free from medium effect interference, is used for quality control and evaluation between the detection systems, and more really reflects actual quality conditions of human serum (plasma) samples, which are detected by detection systems.

The invention relates to an antibacterial bone formation-promoting composite functional porous orthopaedic implant and a preparation method. The preparation method comprises the following steps: (1) preparing a porous orthopaedic implant by utilizing a 3D printing technology; (2) carrying out surface treatment on the porous orthopaedic implant by utilizing a micro-arc oxidation method, and covering the surface of the porous orthopaedic implant with a micro-arc oxidation coating containing calcium and phosphorus elements; (3) soaking the implant treated by the step (2) into 2-5mg / mL dopamine hydrochloride solution, and vibrating for 20-24 hours at the constant temperature of 30-45 DEG C; and (4) by taking deionized water or distilled water as a solvent, adding heparin sodium and preparing aheparin sodium solution with the concentration of 1-5mg / mL, then by taking the heparin sodium solution as a solvent, adding gentamicin and preparing a gentamicin solution with the concentration of 1-5mg / mL; and soaking the implant treated by the step (3) into the gentamicin solution, and vibrating for 4-10 hours at the constant temperature of 30-45 DEG C, so that the implant is obtained.

The invention discloses an artificial diet for predative natural enemy insect-true bugs. The feeding prescription comprises 2-4g of soybean flour, 15-25g of raw pig liver, 24-50g of egg yolk, 30-50ml of water, 7-12g of cane sugar, 0.1-0.3g of vitamine C, 20-50mg of compound vitamin B, and 2-4mg of gentamicin. Each biological indicator or parameter of the stinkbugs fed by the feed disclosed by the invention is mostly the same as the parameter of the stinkbugs fed by tussah pupas; the pre-oviposition period of female fed by the artificial diet is a little longer than that of the female fed by the tussah pupas, and about 4 days longer than that of the female fed by the tussah pupas; however, the feed prescription disclosed by the invention is low in cost and simple in preparation; characters of the fed stinkbugs are similar with those of the stinkbugs fed by the tussah pupas, and the artificial diet is suitable for generalization and application in the industry.

A method of treating prostate cancer in a living mammal includes local administration of a composition that includes a therapeutically effective concentration of collagenase. In one embodiment, a method of treating prostate cancer in a living mammal includes local administration of a composition that includes a therapeutically effective concentration of collagenase and at least one of a glycosidase, a protease, a nuclease, a lipase, an esterase, a plasminogen activator, a streptokinase, and combinations thereof. Preferably a glycosidase, such as, for example, hyaluronidase, is administered. Compositions used in methods for treating prostate cancer can also include or be administered with calcium ions, a nonionic surfactant, such as, for example, Triton® X-100, and an antibiotic, such as, for example, gentamicin. Another method of treating prostate cancer in a living mammal includes activating PSA in vivo by, for example, locally administering calcium ions.

The invention discloses a novel frozen semen diluent used for improving the freeze-thaw quality and fertilization rate of frozen bovine semen and a preparation method thereof. Every 100ml of the prepared frozen semen diluent contains 0.9-1.2g of sodium citrate, 3-5g of sucrose, 18-22ml of yelk, 4-7ml of glycerin, 6-9mg of vitamin E, 500-700mg of vitamin C, 35mg of spectinomycin and 80 thousand units of gentamicin. The invention adds VE and VC into the semen diluent for the first time, so as to increase the motility rate and acrosome integrity of the frozen sperm as well as the activity of antioxidant enzyme in seminal plasma, thus improving the quality and fertilization rate of the freeze-thaw sperm.

The invention belongs to the field of environmental-stimulus-response-type medicines, and particularly relates to a sol system used for preparing pH-sensitive double-network hydrogel, the hydrogel andapplication. The sol system is prepared from gelatin methacryloyl, oxidized alginate, a photoinitiator, gentamicin and mesoporous silica nano particles loaded with phenamil medicinal molecules, the sol system is transformed from sol to gel fast under ultraviolet irradiation, thus the pH-sensitive double-network hydrogel can be obtained, and the hydrogel is excellent in physical and chemical property and good in biocompatibility, can effectively control release of an antibacterial medicine GS and an osteogenic differentiation promoting medicine phenamil, prevent early infection of a graft, induce C2C12 cell osteogenic differentiation, and promote endogenous bone generation. Based on the character that the sol system is transformed from the sol to the gel fast under ultraviolet irradiation,in practical application, in-situ gelation can be conducted by means of minimally-invasive injection, and the requirements of complex orbital bone defect repair can be met, so that the sol system used for preparing the pH-sensitive double-network hydrogel has important values in orbit repair and reconstruction.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

The invention provides a sterilizing method of a spherical phaeocystis culture solution, and relates to a microalgae culture solution. The method comprises the following steps: centrifuging algae solution which grows to an exponential phase, and removing supernatant; carrying out gravity suspension on algae cells with an f / 2 culture medium, centrifuging, and removing supernatant, repeating twice, and carrying out gravity suspension on the algae cells with the f / 2 culture medium; adding SDS (sodium dodecyl sulfate) and antibiotics, wherein the antibiotics are clindamycin, azithromycin, gentamicin, kanamycin, streptomycin, cefotaxime, ampicillin and rifamycin; culturing under illumination, centrifuging the algae solution and removing supernatant during culturing, carrying out gravity suspension on the algae cells with the f / 2 culture medium, centrifuging and removing supernatant, removing residual SDS and the antibiotics, transferring into the f / 2 culture medium, and culturing under illumination; and selecting survival transferred algae solution which is treated with SDS and the antibiotics, and detecting whether bacteria exist or not after the transferred algae solution grows to the exponential phase. The method is convenient to operate, complex operations, such as bacterium separation and test on sensitivity to antibiotics and the like, are avoided, and long-term treatment of high-concentration antibiotics has good sterilization effect.

The present invention comprises compositions for treating diseases caused by nonsense mutations, including dipeptide antibiotics of Formula (I) shown below, such as negamycin. Unlike aminoglycoside antibiotics such as gentamicin, dipeptide antibiotics can induce the expression of mature proteins by readthrough nonsense mutations without generating serious side effects.

The invention discloses a medical composite alginate dressing containing antibacterial drugs and a preparation method thereof. In parts by weight, the medical composite alginate dressing comprises the following components: 50-99.9 parts of alginate and 0.05-10 parts of antibacterial drugs, The antibacterial drug is selected from gentamicin or its salt, neomycin or its salt, amikacin or its salt, fusidic acid or its salt, kanamycin or its salt, polycresol , mupirocin, sulfamethonium or its salt and clindamycin or its salt at least one. The medical composite alginate dressing of the present invention has strong ability to absorb exudate, has good water vapor transmission rate, can be biodegraded and has good biocompatibility, does not adhere to the wound and can quickly form gel after absorbing wound exudate , has strong moisturizing properties, can effectively promote wound healing, shorten wound repair time, can effectively prevent or treat sensitive bacterial infections, and can be widely used in acute and chronic wounds with exudate.

An anti-infection catheter arrangement with catheter (1), which exhibits a rigid or flexible catheter tube with the connection piece (10) furnished at a rear side end, wherein the filling and suction device (1) with several reservoirs of active agent is connectable to the connection piece (10), wherein at least one of the active agent reservoirs is filled with a substance, containing at least one antibiotic, or, respectively chemo-therapeutic agent or, respectively, an anti-viral agent, preferably aminoglycoside, preferably gentamicin (13) in an at least minimum effective concentration, wherein a further one of the reservoirs is filled with a substance containing or forming at least one essentially non-damaging material to the tissue cells and blood cells, preferably an anticoagulant, in particular heparin (12), wherein the filling and suction device (1) is constructed such that this substance essentially can be filled into the region of the tip of the catheter tube (15) and wherein the total volume of the reservoirs corresponds at least to the filling volume of the catheter, possibly additionally to the volume of intermediate pieces, in particular of a three-way cock.

The invention discloses Pseudomonas nitroreducens and application thereof, belonging to the field of environmental microorganism. The Pseudomonas nitroreducens CQ1 of the invention is derived from thesewage of oxidation ponds in the sewage treatment system of Beijing Yanshan Petrochemical Co., Ltd., and obtained by enriching, separating and purifying. The Pseudomonas nitroreducens CQ1 is culturedand collected in the China General Microbiological Culture Collection Center (hereinafter referred to as CGMCC) with the collection number being CGMCC No.3159. The invention further discloses the application of Pseudomonas nitroreducens in the biodegradation of quinoline or derivatives thereof. Quinoline or derivatives thereof with the concentration reaching 700mg / L can be degraded by Pseudomonasnitroreducens of the invention, and the degradability can reach 100% after treating for 72h. Moreover, the Pseudomonas nitroreducens CQ1 of the invention is sensitive to kanamycin sulfate, tetracycline and gentamicin and does not carry plasmids.

The invention provides a flushing fluid for surgery, which comprises solute and solvent, wherein the solute is one or two selected from cellulose glycollic ether, carboxymethyl chitosan or carboxymethyl chitosan, the solvent being water for injection, physiological saline solution, sodium chloride flushing fluid, mannitol flushing fluid, glucose flushing fluid, chlorhexidine flushing fluid, gentamicin physiological saline flushing fluid, active iodine flushing fluid, benzalkonium bromide flushing fluid, aminoacetic acid flushing fluid, metronidazole flushing fluid or physiological equilibrium liquid comprising phosphates cushioning liquid, NaHCO3, sodium gluconate and mannitol.

The invention relates to a neural stem cells medium and a method for performing human neural stem cells in-vitro long-term culture and amplification by using the neural stem cells medium. The neural stem cells medium comprises the following ingredients by weight proportion: 100-1000 micrograms of heparin sodium, 10-100 micrograms of vitamin E, 5-50 milligrams of insulin human recombinant, 0.5-5 milligrams of putrescine, 2-10 micrograms of sodium selenite, 2-10 milligrams of human transferrin, 2-10 micrograms of progestin, 300 milligrams of L-glutamine, 5.9 grams of 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid, 10-100 micrograms of recombinant human epidermal growth factors, 10-100 micrograms of recombinant human basic fibroblast growth factors, 20-200 milligrams of vitamin C glucoside and 40,000-400,000 IU (international unit) of gentamicin. By the neural stem cells medium, the technical problems that human neural stem cells are easy to differentiate when cultured in vitro and long-term culture and amplification are difficult to implement are solved.

The invention relates to preparation and storage of umbilical cord-mesenchymal stem cells (UC-MSCs) used in clinical therapy. The preparation method of the UC-MSCs comprises the following steps: cleaning an umbilical cord and cutting the umbilical cord into granules; putting the granules in physiological saline containing 2 percent gentamicin; carrying out centrifugation, suspension and centrifugation; carrying out cultivation for 10 days; carrying out passage in the proportion of 1:3 to between fourth and fifth generation, and obtaining a great amount of mesenchymal stem cells; and finally, putting the mesenchymal stem cells in liquid nitrogen for storage after adding freezing medium and keeping the freezing temperature of the liquid nitrogen at 196 DEG C below zero.

The invention relates to placenta preserving fluid with pH value of 7.33-7.83; the placenta preserving fluid per 1,000ml contains 10-15mmol of potassium dihydrogen phosphate, 30-40mmol of sodium chloride, 40-50mmol of potassium chloride, 8-10mmol of magnesium sulfate, 70-100mmol of histidine, 1.5-2.0mmol of allopurinol, 3mmol of reduced glutathione, 1-8ml of 2-mercaptoethanol, 8-10mmol of adenosine, 1,000,00-1,000,000U of penicillin, 60-100mg of streptomycin and 50-70mg of gentamicin. The placenta preserving fluid can store the delivered placenta for a long time, prevent from reduction of cell activity and control the bacteria contamination rate of the placenta to be in a low level.

The present invention relates to the field of bacteriology. In particular, the present invention provides compositions (e.g., comprising a lantibiotic and mupirocin or gentamicin) and methods of treating (e.g., killing or inhibiting growth of) bacteria. For example, the present invention provides pharmaceutical compounds (e.g., comprising a lantibiotic and mupirocin or gentamicin) and methods of using the same in research, therapeutic and drug screening applications.

An antimicrobial composition comprising: a complex of a polysaccharide covalently bonded with an antibiotic. A medical device having an antimicrobial composition comprising: a complex of an oxidized regenerated cellulose covalently bonded with gentamicin.

The invention relates to an NK cell in-vitro amplification culture medium combination and a culture method. The culture medium combination comprises a base culture medium, an induced culture medium, a proliferation culture medium and an activated adaptive culture medium, wherein the base culture medium is prepared from the following components: an RPMI-1640 (Roswell Park Memorial Institute-1640) culture medium, a GT-T551 culture medium, gentamicin and BSA (Bovine Serum Albumin); the induced culture medium is prepared from the following components: the RPMI-1640 culture medium, the GT-T551 culture medium, the BSA, lentinan, ganoderma lucidum polysaccharides, tea polyphenols, allicin and paclitaxel; the proliferation culture medium is prepared from the following components: the RPMI-1640 culture medium, the GT-T551 culture medium, the BSA and interleukins; and the activated adaptive culture medium is prepared from the following components: the RPMI-1640 culture medium, the GT-T551 culture medium, insulin, transferrin and glutamine. According to the technical scheme, the quantity of NK cells cultured by the NK cell in-vitro amplification culture medium combination and the method is more, and the NK cells also have higher activity in clinical treatment.

The invention provides a preparation method of long-acting boar semen diluting powder. According to the method, 26-36 g of glucose, 3-7 g of dry chicken egg-yolk powder, 3-6 g of sodium citrate, 1.5-2.5 g of aminoacetic acid, 1.0-2.0 g of disodium hydrogen phosphate, 1.0-2.0 g of ethylene diamine tetraacetic acid disodium, 2.0-4.0 g of potassium sorbate, 1.0-2.0 g of calcium propionate, and 0.005-0.02 g of gentamicin powder are taken; the powdery raw materials taken according to the previous quantities are evenly mixed together so that the boar semen diluting powder is obtained. The preparation method of the dry chicken egg-yolk powder comprises the following steps: cooking a fresh chicken egg, taking out the egg yolk and drying the egg yolk to obtain dry chicken egg-yolk powder with the moisture content of 1-10%. The nutrient solution for diluting boar semen prepared by the raw materials provided in the method and distilled water contains a nutritional agent, a diluting agent, a buffering agent, a pH regulator and an antibiotic, as well as a preservative and a bacteriostatic agent, and can be preserved for 0-12 days under the condition of a constant temperature ranging from 15 to 20 DEG C.

The invention provides an enzyme linked immune regent box to detect gentamycin. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the gentamycin (when the antigen is on enzyme labeled board and the enzyme marker is the enzyme labeled antiantibody or the antiantibody is in the enzyme labeled board and the enzyme marker is the enzyme labeled antigen), the gentamycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the gentamycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

This invention protects the use of nicotine, analogues thereof precursors thereof or its derivates for treatment of inflammatory, infectious, candidal or degenerative (of the joints and / or of the central nervous system, of kidneys, the lungs, liver), depression, obesity, bone disease and the like, which can be improved by means of intensification of the actions of α-MSH, given the fact that this hormone are extraordinary properties: e.g., it has an antipyretic potency 20,000 times as great as acetaminophen, its antimicrobian potency, is comparable to gentamycine, it is the best anticandidiasic known; it inhibits apoptosis of various stem cells, and significantly modulates the immune reactions, and therefore the use of agents that affect its release may have significant therapeutic potential. This patent protects the use of nicotine, analogues thereof, precursors thereof or its derivates for the purpose of increasing and / or reducing the bioavailability of α-MSH in blood and / or central or peripheral tissues to accentuate or diminish the effect of the α-MSH by means of changes in its concentration or its effect on the corresponding receptors of any cell, tissue or organ in the body, administrated for therapeutic and / or prophylactic purposes in the short medium and / or long term.