70 results about "Proclin" patented technology

The invention provides a human blood fat (serum / plasma) quality management reference kit. The kit takes human serum (plasma) as a matrix; a human blood fat component (human apolipoprotein A1 antigen, apolipoprotein B antigen, high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL) and the like), a stable system and an anticorrosion system are added to the matrix; the stable system is any combination of a surfactant of Triton series, Tween series, EDTA (ethylene diamine tetraacetic acid) series and the like, a polymer of polyvinyl alcohol series, polyvinylpyrrolidone series, polyethylene glycol series and the like, and a phosphate buffer solution; and the anticorrosion system is any combination of sodium azide, gentamicin, amphomycin and ProClin series. The invention further provides a preparation method of the kit. The kit is free from medium effect interference, is used for quality control and evaluation between the detection systems, and more really reflects actual quality conditions of human serum (plasma) samples, which are detected by detection systems.

The invention provides a blocking buffer for encapsulated plate, which consists of 0.5 to 10g / L of protein substances, 0.5 to 5g / L of sugar substances, 0.5 to 10g / L of amino acid substances, 0.2 to 2g / L of surfactant, 50 to 5 million unit / L of gentamicin sulphate, 0.2 to 2g / L of Proclin 300 and 0.02mol / L of phosphate buffer with the pH of 7.4. The encapsulated plate using the blocking buffer of the invention can not only block off biological active substances, but can also maintain the activity of the biological active substances for a longer time when the encapsulated plate departs from low temperature environment, thus the problem in relevant technology that the encapsulated plate is liable to lose activity when departing from lower temperature storage environment can be settled. In addition, the blocking buffer of the invention is easy in formulation, free from the impact on sensitivity, low in cost and simple in operation and has no environmental pollution and no toxicity and harm to human body.

The invention relates to a lipoprotein (a) detection kit which comprises a reagent R1, a reagent R2 and a reference substance, wherein the reagent R1 is an HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer system which comprises 0.5-10 g / L HEPES, 2-20 g / L sodium chloride, 0.05-1.0 ml / L Tween-20, 0.1-2 g / L bovine serum albumin, 5-25 g / L polyethyleneglycol-6000, 1-5 g / L EDTA (ethylene diamine tetraacetic acid) and 0.1-2 g / L Proclin 300; the reagent R2 comprises a polystyrene latex particle mixture which is prepared by a chemical crosslinking method, coated with an anti-human-apolipoprotein (a) polyclonal antibody and provided with carboxylic groups on the surface, a 0.5-10 g / L HEPES buffer solution and aspartame, wherein the weight-to-volume ratio of the aspartame to the reagent R2 is (0.01-0.5):100 (g / L); and the reference substance is a human serum or serum-matrix-like liquid with human recombinant apolipoprotein (a). The kit provided by the invention has the advantages of high detection sensitivity, high accuracy, favorable precision, favorable linearity within detection range, high stability, low production cost and low blank absorbance, and has anti-interference performance.

The invention relates to a neuron-specific enolase stabilizer. The stabilizer is prepared from a Tris.HCl buffer solution, NaCl, sucrose, trehalose, casein, bovine serum albumin, PEG6000, lysine, disodium ethylene diamine tetraacetate, dithiothreitol, glycerin, Tween-20, Triton X-100, aprotinin with the final concentration of 100-300 KIU / mL, and Proclin 300, and above substances are uniformly mixed in Millipore ultrapure water used as a solvent, is filtered by a 0.22 [mu]m filter membrane, and finally is preserved at 0-10 DEG C. The stabilizer greatly improves the preservation stability of neuron-specific enolase, prolongs the shelf life of a kit, simplification of the production technology and the doctor's detection operation process of a neuron-specific enolase detection kit, improvement of the metamorphism resistance in the preparation, transportation and storage process of the neuron-specific enolase, and reduction of inter-assay variation caused by a freeze-drying technology.

The invention discloses a detection kit for LDL (low-density lipoprotein) cholesterol and a use method of the detection kit, relates to the field of biochemical detection and aims to provide a detection kit, having high stability, accuracy and precision as well as low toxicity, for LDL cholesterol and a use method of the detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from a surfactant, polyethylene glycol, SDS (sodium dodecyl sulfate), Emulgen A9 series, CHOD (cholesterol oxidase), CHER (cholesterol esterase), ascorbic acid oxidase, CAT (catalase), 4-AAP (4-aminoantipyrine) and the like; the reagent 2 is prepared from octylphenyl polyethylene glycol, cholate, a compound stabilizer, bovine serum albumin, POD (peroxidase), TOPS (sodium 3-(N-ethyl-3-methylanilino)propanesulfonate) and the like. The proclin series added to the kit is a novel biological preservative, has good compatibility with various enzymes, better stability and low toxicity, and the stability of the reagents is maintained.

The invention relates to a free fatty acid determination reagent kit which comprises dual reagents. The reagent 1 comprises Good's buffer solution (pH(potential of hydrogen)=7.0), coenzyme A, ATP (adenosine triphosphate), acetylcoenzyme A synthetase, magnesium chloride (MgCL2), Trinder bound constituent, tween 80 and liquid biological preservative / Proclin 300, and the reagent 2 comprises Good's buffer solution (pH=7.0), acetylcoenzyme A oxidase (ACOD), peroxidase (POD), Trinder bound constituent, tween 80 and liquid biological preservative / Proclin 300. The free fatty acid determination reagent kit has the remarkable advantages that the reagent kit is directly used for an automatic biochemical analyzer and is simple; and the adopted automatically separated and purified acetylcoenzyme A synthetase and acetylcoenzyme A oxidase (ACOD) have high specificity on free fatty acid in serum.

The invention discloses a Alpha1-AT (antitrypsin) immunoturbidimetry detection kit and belongs to the technical field of clinical external detection reagents. The detection kit comprises a reagent R1, a reagent R2 and a calibrator. A certain amount of magnetic nano-particles covered with silicon dioxide are added in the reagent R1, a certain amount of BSA (bovine serum albumin) and Proclin-300 are added in the reagent R2, and accordingly analytical sensitivity and stability of the detection kit are improved, linear range thereof is expanded, reagents accuracy is higher, and further application and promotion in the market is facilitated.

The invention belongs to the technical field of biological in vitro diagnostic reagents and particularly relates to a confining liquid for an enzyme linked immunosorbent assay in vitro diagnostic reagent. The confining liquid is prepared by the following technological steps: preparing PBS (Phosphate Buffer Solution); adding albumin bovine serum to be dissolved fully; adding trehalose to be dissolved fully; adding protein stabilizing agent to be dissolved fully, adding L-lysine to be dissolved fully; adding Proclin 300 to be mixed fully; adding sodium hydroxide solution or hydrochloric acid solution for adjusting the pH value to be mixed fully; and standing for more than 30 min at room temperature and preserving at 2-8 DEG C. Compared with the prior art, the confining liquid has a broad spectrum activity, can inhibit the growth of microbe like bacteria, fungi and microzyme for a longer time, and meanwhile can retain the activity of enzyme in the system.

The invention discloses a kit for measuring content of total bile acid of blood serum and a use method of the kit, relates to the field of biochemical detection and aims to provide a high-stability, high-accuracy and non-interfering kit for measuring the content of total bile acid of blood serum and a use method of the kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises a GOOD'S buffer solution, thionicotinamide adenine dinucleotide, polyoxyethylene ether of nonylphenol, polyoxyethylene lauryl ether, ADP.2Na, EDTA (ethylene diamine tetraacetic acid), bovine serum albumin, mannitol and a preservative (proclin series); the reagent 2 comprises a GOOD'S buffer solution, 3 alpha-hydroxysteroid dehydrogenase, reduced coenzyme and the like. Due to addition of ethylene glycol, sucrose and multiple compound stabilizers, the stability of the reagents is improved; due to addition of EDTA, the interference effect of metal ions can be reduced and the test result accuracy can be improved.

The invention discloses a fecal occult blood latex enhanced immunoturbidimetric kit comprising a R1 reagent, a R2 reagent and a human hemoglobin calibrator; the R1 reagent comprises a glycine buffer,PEG6000 and ProClin-300; the R2 reagent comprises a glycine buffer, rabbit anti-human hemoglobin-coated latex microspheres, bovine serum albumin, Tween-20, trehalose, ProClin-300; the latex microspheres in the reagent R2 have a particle size of 60 to 220 nm, and a hemoglobin antibody is rabbit anti-human hemoglobin polyclonal antibody, and is linked to the polystyrene latex microspheres by covalent coupling. Latex enhanced immunoturbidimetric detection results objectively reflect the amount of bleeding, the fecal occult blood latex enhanced immunoturbidimetric kit facilitates observing of changes in conditions of diseases; due to a full automatic operation mode, the detection process is more convenient and quick, and the fecal occult blood latex enhanced immunoturbidimetric kit can test inbatches, and is more suitable for screening experiments of large-scale populations.

The invention discloses an antigen stabilizer and a preparation method and application thereof. The antigen stabilizer comprises, by weight, 100 parts of Tris-HCl buffer solution, 1-5 parts of bovine serum albumin, 1-10 parts of glycerin, 1-10 parts of mannitol, 0.1-0.5 part of beta-mercaptoethanol and 0.01-1 part of Proclin-300. The antigen stabilizer can stabilize protein, inhibit protein oxidation, resist corrosion, avoid biological-source pollution and lower cost.

The invention provides a protein chip stabilizing agent as well as a preparation method and application thereof. The protein chip stabilizing agent is prepared from saccharose, bovine serum albumin, Tween-20, trehalose and a biological preservative Proclin 300 in a PBS buffer system. The protein chip stabilizing agent prepared by the preparation method is capable of prolonging the protein immune activity storage life and reducing the cost, is safe and effective and contains no other chemical product; the saccharose and the trehalose are remarkably in synergistic effect, the stability of each of the saccharose and the trehalose can be respectively coordinated, and the stability of a whole protein chip can be more effectively increased; the protein immune activity of the protein chip in a kit during the storage life cannot be changed; a formula is simple, the cost is low, the application range is wide, no potential safety hazard exists, and good stabilizing effect and a wide application prospect are obtained.

The invention relates to a novel saliva preserving fluid comprising the following components: 1-10mmol / L of EDTA, 1-50mmol / L of Tris-HCl, 10-100 mmol / L of sodium chloride; 0.1-3% (w / v) of sodium sorbate, 0.1-3% (w / v) of sodium diacetate, 0.01-0.1% (v / v) of proclin 300, 0.1-1% (v / v) of TWEEN20, 0.1-1% (w / v) of NP40 and 0.1-2% (w / v) of SDS. The novel saliva preserving fluid is used for preservingsaliva samples, can effectively inhibit nuclease activity, prevent microbial pollution, guarantee DNA fragment integrity and purity, simplify pretreatment steps, is suitable for centrifuge-free and low-temperature environments, can guarantee transport stability of samples among different places, and is suitable for molecular epidemiological large-scale population investigation and research.

The invention discloses composite cleaning liquid for a chemiluminescent instrument pipeline and a preparation method thereof. The composite cleaning liquid comprises the following components in partsby weight: 36 to 67 parts of sodium hydrogen phosphate, 33 to 55 parts of monopotassium phosphate, 12 to 21 parts of zinc chloride, 15 to 27 parts of sodium lauryl sulfate, 5 to 11 parts of glycerol,0.2 to 0.8 parts of potassium hydrogen phthalate, 0.1 to 0.4 parts of sodium hydroxide, 0.5 to 1 part of gentamicin, 39 to 61 parts of 2-morpholineethanesulfonic acid, 28 to 50 parts of dipotassium hydrogen phosphate, 11 to 29 parts of citric acid, 17 to 25 parts of polyethylene glycol, 4 to 8 parts of bovine serum albumin, 12 to 21 parts of sucrose, 0.5 to 1 part of Tween-20, 0.2 to 1 part of Proclin 300, and 300 to 500 parts of deionized water. The method utilizes the synergistic effect among the components to obtain the composite cleaning liquid for the chemiluminescent instrument pipeline, The preparation method of a diluent is simple, and is suitable for large-scale production in a factory. The cleaning liquid has good effect, little damage to the pipeline, and is suitable for long-term storage.

The invention aims to provide a reagent for quantitatively detecting ractopamine by a latex enhanced immune turbidimetry. The reagent comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared by adding distilled water into 3.75-15.01 g of glycine and 0.2-1.1 g of a ractopamine-carrier antigen complex and fixing a volume to 1000 ml; the reagent R2 is prepared by adding distilled water into 1.4-7.0 g of glycine, 10-30 g of polyethylene glycol 6000, 100-600 mg of carboxyl latex and anti-ractopamine monoclonal antibody cross-linked latex, 10-30 g of bovine serum albumin (BSA) and 1-5 m of Proclin 300 and fixing a volume to 1000 ml. The reagent provided by the invention is scientific and reasonable in formula, easy to produce and prepare, and convenient, safe and accurate to use, can be effectively used for quickly detecting the ractopamine in food, ensures the food safety and has significant economic and social benefits.

The invention provides a novel nano magnetic particle suspension system and a preparation method thereof. According to the suspension system, with purified water as a solvent, every 1L of the suspension system contains 0.1-5 g of sodium phosphate dibasic dodecahydrate, 0.1-1 g of sodium dihydrogen phosphate dihydrate, 5-50 g of sodium chloride, 0.01-1.0 g of 4-aminoantipyrine, 1-50 g of bovine serum albumin, 10-50 mL of isopropanol, 0.01-0.5 mL of Triton X-100 and 0.1-5 mL of Proclin-300. The mass concentration of magnetic particles in the suspension system is 5.0%. The novel nano magnetic particle suspension system has good dispersion performance, the magnetic particles are evenly distributed, and the good dispersion and stabilization performance can be guaranteed; under the effect of themagnetic field, the nano magnetic particles can quickly settle, solid and liquid separation is quickly achieved, and the system has good paramagnetism.

The invention provides a diluent capable of improving the stability of an acridinium ester antigen-antibody conjugate and reducing the background. The diluent comprises the components of 5 to 7g of Tris-base, 3.5 to 4.0 mL of concentrated hydrochloric acid, 9 to 11 g of polyethylene glycol-6000, 0.95 mL to 1.05 mL of 10% lauryl sodium sulfate, 9.5 mL to 10.5 mL of Tween, 0.8 to 1.0 g of ethylene diamine tetraacetic acid disodium salt, 9 to 11 g of sodium caseinate, 0.95 mL to 1.05 mL of Proclin 300, 9.5 to 10.5 mL of triton X-100, and 1000mL of purified water for an in-vitro diagnostic reagent. The diluent has the advantages that the components are few; polyethylene glycol 6000, sodium dodecyl sulfate, tween and several active agents are mixed for use, meanwhile, a Tris-base buffer solution system is used, and multiple raw materials play a mutual synergistic effect, so that the diluent has a better protection effect on acridinium ester labeled antigens and antibodies, can be stored for a long time, can provide good blocking property and protectiveness for the antibodies, and reduces non-specific reactions; meanwhile, the material cost is saved, the specificity, stability, repeatability and other properties of the reagent are not influenced, and the reagent is suitable for popularization and application.

The invention belongs to a clinical medicine detection technology, and particularly relates to a plasma fibrinogen detection reagent, a plasma fibrinogen detection method, and applications of the plasma fibrinogen detection reagent, wherein the plasma fibrinogen detection reagent comprises a plasma diluent R1 and a thrombin buffer system R2, the plasma diluent R1 comprises imidazole, NaCl and polyethylene glycol, and the thrombin buffer system R2 comprises imidazole, Triton-X 100, mannitol, glycine, trehalose, bovine serum albumin, Proclin-300 and thrombin. According to the present invention,the detection method has advantages of high precision and simple operation; and the safe, reliable and good-repeatability in vitro diagnostic reagent is provided for the clinical detection of plasma fibrinogen.

The invention provides a stable reagent for determining glutamic oxalacetic transaminase. The reagent is a dual reagent and comprises TRIS (tris (hydroxymethyl) aminomethane), NADH (reduced form of nicotinamide-adenine dinucleotid), LDH (lactate dehydrogenase), MDH (malate dehydrogenase), EDTA (ethylene diamine tetraacetic acid), TritonX-100, BSA (bovine serum albumin) and Proclin. According to the reagent, the heat stability, onboard stability and transportation stability of an in-vitro diagnosis reagent of an NADH indicating system are effectively improved; and the shelf life of a reagent kit can be prolonged effectively.

The invention provides a double-antibody sandwich enzyme-linked immunosorbent assay kit for detecting the content of anti-mullerian hormone and a detection method of the double-antibody sandwich enzyme-linked immunosorbent assay kit. The kit comprises an elisa plate coated with a capture antibody, a sample diluent, a standard substance, a biotin-labeled detection antibody, horseradish peroxidase-labeled avidin, a concentrated washing solution, a substrate solution and a stop solution, wherein the capture antibody is a mouse anti-human AMH antibody; the sample diluent is prepared from 1 * PBS,1% of BSA, 0.05% of Tween-20 and 0.1% of Proclin 300; the standard substance is a human AMH freeze-dried standard substance; the detection antibody is an anti-human AMH antibody; the concentrated washing liquid is 25 * PBST containing 25 * PBS, 1.25% of Tween 20 and 2.5% of Proclin 300, and the pH value of the concentrated washing liquid is 7.6; the substrate solution is a TMB chromogenic substrate solution; and the stop solution is 2M sulfuric acid. According to the kit and the detection method thereof, the manpower and equipment costs are reduced, the usage amount of raw materials and samples is reduced, and the cost is reduced, so that the use efficiency is improved.

The invention provides a stable reagent for determining alanine aminotransferase. The reagent is a dual reagent including TRIS, NADH, LDH, EDTA, TritinX-100, BSA, and Proclin. Through the reagent, thethermal stability, airboard stability and transportation stability of an in vitro diagnostic reagent of an NADH indicating system can be effectively improved, and the storage time of a kit can be effectively prolonged.

The invention discloses an in-vitro diagnostic kit for measuring prothrombin time. The in-vitro diagnostic kit comprises a prothrombin time measuring reagent and a plastic bottle for containing the prothrombin time measuring reagent, wherein the prothrombin time determination reagent is composed of a buffer solution system and thromboplastin, and the thromboplastin is obtained by taking rabbit brain powder as a raw material through extraction; the buffer solution system is prepared from the following raw materials: a buffer agent, a calcium ion reagent, a surfactant, amino acid, trehalose andprocline 300. In the kit disclosed in the present invention, the buffer solution system is creatively prepared by compounding the buffer agent, the calcium ion reagent, the surfactant, the amino acid,the trehalose and the proclin 300; through mutual cooperation and synergy with thromboplastin, the stability of the prothrombin time determination reagent can be improved on the basis of ensuring theaccuracy and sensitivity of the prothrombin time determination reagent.

The invention discloses an adsorbent for detecting an IgM antibody. A phosphate buffer solution of 10mM of PB and 150mM of NaCl and with the pH of 7.2 to 7.4 is taken as a base, polyethylene glycol 6000 of which the final concentration is 6wt%, 1mg / mL of an anti-human IgG antibody, 10mg / mL of BSA and 0.1wt% of proclin 300 are added, and the titer of the IgG antibody is not less than 1: 300000. The adsorbent is simple in reagent composition, and cheap in price of the anti-human IgG and PEG6000 which are taken as main reagents and mild in reaction, the pH of a buffer system of the adsorbent leans neutrality, and the ionic strength is suitable, thereby having little effect on the immune response of an antigen antibody; the adsorbent further has better sensitivity and specificity, a better absorption effect on specific IgG and RF factors and a good application range for most of IgM detection reagents, especially for indirect-method determination of IgM, and can obviously lower sample backgrounds which affect detected IgM antibody results and improve the detection accuracy.

The invention discloses a multifunctional antibody dilution solution and a preparation method thereof, and the multifunctional antibody dilution solution comprises, by weight, 1-3 parts of casein sodium, 0.1-0.5 part of gentamicin, 38-67 parts of sodium bicarbonate, 33-55 parts of sodium citrate, 11-21 parts of citric acid, 9-15 parts of p-aminobenzenesulfonamide, 7-18 parts of potassium chloride,6-13 parts of sodium chloride, 2-8 parts of cystine, 11-19 parts of sucrose, 0.3-1 part of sodium hydroxide, 0.1-0.6 part of bovine serum albumin, 0.1-0.7 part of goat IgG, 0.3-0.7 part of Tween 40,0.1-0.5 part of Proclin 300 and 200-400 parts of deionized water. The multifunctional antibody dilution solution is prepared by the synergistic action of the components. The multifunctional antibody dilution solution is simple in preparation method, and can provide good blocking and protection for an antibody so as to reduce the non-specific reaction and improve the stability of the antibody.

The invention provides a stable reagent for measuring aspartate aminotransferase. The reagents of the present disclosure are dual reagents comprising TRIS, NADH, LDH, MDH, EDTA, Triton X-100, BSA, Proclin. The disclosed reagent can effectively improve the thermal stability, airborne stability and transportation stability of the NADH indicator system in vitro diagnostic reagent, and can effectively prolong the storage time of the kit.

The invention discloses a single particle size based reagent kit capable of simultaneously detecting a retionl binding protein in blood serum and urine samples. The reagent kit comprises a reagent R1,a reagent R2, a calibration product A, and a calibration product B, wherein the reagent R2 comprises emulsion-polyclonal antibody cross-linking product and a reagent R2 buffer solution and the emulsion-polyclonal antibody cross-linking product accounts for 0.05-1.0wt% of the reagent R2 buffer solution; the reagent R2 buffer solution comprises the following ingredients: 50-120mM Hepes buffer solution, 0.1% of Proclin 300, 1-5% of sucrose, 1-5% of NaCl, 0.1-1% of triton and the balance of purified water; and a pH (potential of hydrogen) of the reagent R2 buffer solution is 6.5-8.5. The reagentkit has the advantages that the reagent kit detects blood and urine simultaneously, and is simple to operate, high in sensitivity, wide in linear range, and low in cost (only a single particle size and a polyclonal antibody can meet detection requirements, and an emulsion-polyclonal antibody can meet marking requirements only by single centrifugation during preparation), and market popularizationis facilitated. At the same time, the detection reagent kit is compatible with most biochemical detection equipment on a market and can be put on the market directly.

The invention discloses a sample diluent applicable to peripheral blood detection. The sample diluent per 1,000ml is prepared from the ingredients: 20.5g of glucose, 8.5g of sodium chloride, 2g of bovine serum albumin, 0.55g of citric acid, 0.4ml of Proclin 300 and 2ml of gentamicin sulfate. The sample diluent is readily available in required raw materials, low in price and free of blood serum ingredient, the risk of infecting some special viruses is avoided, the osmotic pressure of solutions inside and outside cells is balanced by various ions, and the preservation effect is remarkable. The optimized sample diluent is high in applicability, wide in range of application and superior in oxidation resistance and can exert a protecting action during the transportation and preservation of peripheral blood diluted samples.

The invention relates to the technical field of cell chromogenic reagents, in particular to an immune cell chemical labeling chromogenic kit for auxiliary diagnosis of cervical cancer. The kit comprises a ready-to-use antibody working solution, a reagent solution C, a reagent solution D, a reagent solution E, a reagent solution F and a reagent solution G; the ready-to-use antibody working solution comprises an antibody reagent, bovine serum albumin, non-reducing sugar, fish skin gelatin, a preservative proclin-950, tender leaf green pigment, polyethylene glycol, casein, a surfactant, 4-aminoantipyrine and sodium chloride, and the antibody reagent comprises a reagent solution A and a reagent solution B. The kit can be used for cervical decellularization and can also be used for tissue staining. The blue returning mode is newly created, the blue returning effect is better than that of kits of other manufacturers in the market, the sheet sealing mode is simpler, and the experiment time is greatly shortened.