70 results about "Proclin" patented technology

The invention provides a human blood fat (serum / plasma) quality management reference kit. The kit takes human serum (plasma) as a matrix; a human blood fat component (human apolipoprotein A1 antigen, apolipoprotein B antigen, high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL) and the like), a stable system and an anticorrosion system are added to the matrix; the stable system is any combination of a surfactant of Triton series, Tween series, EDTA (ethylene diamine tetraacetic acid) series and the like, a polymer of polyvinyl alcohol series, polyvinylpyrrolidone series, polyethylene glycol series and the like, and a phosphate buffer solution; and the anticorrosion system is any combination of sodium azide, gentamicin, amphomycin and ProClin series. The invention further provides a preparation method of the kit. The kit is free from medium effect interference, is used for quality control and evaluation between the detection systems, and more really reflects actual quality conditions of human serum (plasma) samples, which are detected by detection systems.

The invention relates to a lipoprotein (a) detection kit which comprises a reagent R1, a reagent R2 and a reference substance, wherein the reagent R1 is an HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer system which comprises 0.5-10 g / L HEPES, 2-20 g / L sodium chloride, 0.05-1.0 ml / L Tween-20, 0.1-2 g / L bovine serum albumin, 5-25 g / L polyethyleneglycol-6000, 1-5 g / L EDTA (ethylene diamine tetraacetic acid) and 0.1-2 g / L Proclin 300; the reagent R2 comprises a polystyrene latex particle mixture which is prepared by a chemical crosslinking method, coated with an anti-human-apolipoprotein (a) polyclonal antibody and provided with carboxylic groups on the surface, a 0.5-10 g / L HEPES buffer solution and aspartame, wherein the weight-to-volume ratio of the aspartame to the reagent R2 is (0.01-0.5):100 (g / L); and the reference substance is a human serum or serum-matrix-like liquid with human recombinant apolipoprotein (a). The kit provided by the invention has the advantages of high detection sensitivity, high accuracy, favorable precision, favorable linearity within detection range, high stability, low production cost and low blank absorbance, and has anti-interference performance.

The invention provides a protein chip stabilizing agent as well as a preparation method and application thereof. The protein chip stabilizing agent is prepared from saccharose, bovine serum albumin, Tween-20, trehalose and a biological preservative Proclin 300 in a PBS buffer system. The protein chip stabilizing agent prepared by the preparation method is capable of prolonging the protein immune activity storage life and reducing the cost, is safe and effective and contains no other chemical product; the saccharose and the trehalose are remarkably in synergistic effect, the stability of each of the saccharose and the trehalose can be respectively coordinated, and the stability of a whole protein chip can be more effectively increased; the protein immune activity of the protein chip in a kit during the storage life cannot be changed; a formula is simple, the cost is low, the application range is wide, no potential safety hazard exists, and good stabilizing effect and a wide application prospect are obtained.

The invention discloses composite cleaning liquid for a chemiluminescent instrument pipeline and a preparation method thereof. The composite cleaning liquid comprises the following components in partsby weight: 36 to 67 parts of sodium hydrogen phosphate, 33 to 55 parts of monopotassium phosphate, 12 to 21 parts of zinc chloride, 15 to 27 parts of sodium lauryl sulfate, 5 to 11 parts of glycerol,0.2 to 0.8 parts of potassium hydrogen phthalate, 0.1 to 0.4 parts of sodium hydroxide, 0.5 to 1 part of gentamicin, 39 to 61 parts of 2-morpholineethanesulfonic acid, 28 to 50 parts of dipotassium hydrogen phosphate, 11 to 29 parts of citric acid, 17 to 25 parts of polyethylene glycol, 4 to 8 parts of bovine serum albumin, 12 to 21 parts of sucrose, 0.5 to 1 part of Tween-20, 0.2 to 1 part of Proclin 300, and 300 to 500 parts of deionized water. The method utilizes the synergistic effect among the components to obtain the composite cleaning liquid for the chemiluminescent instrument pipeline, The preparation method of a diluent is simple, and is suitable for large-scale production in a factory. The cleaning liquid has good effect, little damage to the pipeline, and is suitable for long-term storage.

The invention aims to provide a reagent for quantitatively detecting ractopamine by a latex enhanced immune turbidimetry. The reagent comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared by adding distilled water into 3.75-15.01 g of glycine and 0.2-1.1 g of a ractopamine-carrier antigen complex and fixing a volume to 1000 ml; the reagent R2 is prepared by adding distilled water into 1.4-7.0 g of glycine, 10-30 g of polyethylene glycol 6000, 100-600 mg of carboxyl latex and anti-ractopamine monoclonal antibody cross-linked latex, 10-30 g of bovine serum albumin (BSA) and 1-5 m of Proclin 300 and fixing a volume to 1000 ml. The reagent provided by the invention is scientific and reasonable in formula, easy to produce and prepare, and convenient, safe and accurate to use, can be effectively used for quickly detecting the ractopamine in food, ensures the food safety and has significant economic and social benefits.

The invention provides a stable reagent for measuring aspartate aminotransferase. The reagents of the present disclosure are dual reagents comprising TRIS, NADH, LDH, MDH, EDTA, Triton X-100, BSA, Proclin. The disclosed reagent can effectively improve the thermal stability, airborne stability and transportation stability of the NADH indicator system in vitro diagnostic reagent, and can effectively prolong the storage time of the kit.

The invention discloses an antigen stabilizer and a preparation method and application thereof. The antigen stabilizer comprises, by weight, 100 parts of Tris-HCl buffer solution, 1-5 parts of bovine serum albumin, 1-10 parts of glycerin, 1-10 parts of mannitol, 0.1-0.5 part of beta-mercaptoethanol and 0.01-1 part of Proclin-300. The antigen stabilizer can stabilize protein, inhibit protein oxidation, resist corrosion, avoid biological-source pollution and lower cost.