Single particle size based reagent kit capable of simultaneously detecting retionl binding protein in blood serum and urine samples

Abstract

The invention discloses a single particle size based reagent kit capable of simultaneously detecting a retionl binding protein in blood serum and urine samples. The reagent kit comprises a reagent R1,a reagent R2, a calibration product A, and a calibration product B, wherein the reagent R2 comprises emulsion-polyclonal antibody cross-linking product and a reagent R2 buffer solution and the emulsion-polyclonal antibody cross-linking product accounts for 0.05-1.0wt% of the reagent R2 buffer solution; the reagent R2 buffer solution comprises the following ingredients: 50-120mM Hepes buffer solution, 0.1% of Proclin 300, 1-5% of sucrose, 1-5% of NaCl, 0.1-1% of triton and the balance of purified water; and a pH (potential of hydrogen) of the reagent R2 buffer solution is 6.5-8.5. The reagentkit has the advantages that the reagent kit detects blood and urine simultaneously, and is simple to operate, high in sensitivity, wide in linear range, and low in cost (only a single particle size and a polyclonal antibody can meet detection requirements, and an emulsion-polyclonal antibody can meet marking requirements only by single centrifugation during preparation), and market popularizationis facilitated. At the same time, the detection reagent kit is compatible with most biochemical detection equipment on a market and can be put on the market directly.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a kit based on a single particle size and capable of simultaneously detecting retinol binding proteins in serum and urine samples. Background technique [0002] Retinol Binding Protcin (RBP) is a transporter of retinol (vitamin A) in the blood. RBP is mainly synthesized by liver cells and is widely distributed in human serum, cerebrospinal fluid, urine and other body fluids. In the blood, RBP, retinol and prealbumin exist as a 1:1:1 (mol) complex, transporting 90% of the retinol in the body to the body tissues, when RBP binds to the RBP receptor on the cell surface When retinol enters the cell, the complex is disintegrated, and free RBP is filtered out from the glomerulus, most of which are reabsorbed by the proximal tubular epithelial cells and decomposed. [0003] Studies have shown that retinol binding protein is of great significance in the diagnosis of hypertension and early diabetic...

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to solve the low detection sensitivity (can only detect RBP in serum) or the high production cost (can detect RBP in serum and urine simultaneously on the market at present, but the production cost based on the technical scheme of double particle size is relatively low). High) technical defect, provide a kind of kit based on single particle size and can detect retinol binding protein in serum and urine sample simultaneously

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