1696results about How to "Wide linear range" patented technology

The invention relates to a liquid phase chip for joint detection of multiple tumor markers and a preparation method thereof. The liquid phase chip comprises micro-balloons of a coupled antibody (at least two of the followings: AFP, CEA, CA125, CA153, CA19-9, CA242, CA72-4, PSA, HGH, Beta-HCG), corresponding biotin-labeled detection antibodies, streptavidin phycoerythrin and vegetable hydrosol or polysaccharide hydrosol which has a solute content of 1-10 wt per thousand and does not contain protein. The preparation of the liquid phase chip comprises refining and purification of hydrosol, coupling between captured antibodies and micro-balloons, preparation of biotin-labeled antibodies, dispersing the coupled micro-balloons into the vegetable hydrosol or the polysaccharide hydrosol, and the like. The liquid phase chip has the advantages of stable performance, good micro-balloon dispersivity, long preservation time, fast and convenient use and operation, small amount of samples in use, high detection sensitivity, wide linear scope and low detection cost, can detect ten tumor markers at most at one time, and requires a cost which is a quarter of the total fee of conventional methods.

A method for determining chemical oxygen demand of a water sample comprises the steps of (a) applying a constant potential bias to a photoelectrochemical cell, having a photoactive working electrode (e.g. a layer of titanium dioxide nanoparticles coated on an inert conductive substrate) and a counter electrode, and containing a supporting electrolyte solution; (b) illuminating the working electrode with a light source and recording the background photocurrent produced at the working electrode from the supporting electrolyte solution; (c) adding a water sample, to be analyzed, to the photoelectrochemical cell; (d) illuminating the working electrode with a light source and recording the total photoelectrocurrent produced with the sample; (e) determining the chemical oxygen demand according to the type (exhaustive or non-exhaustive) of degradation conditions employed.

The invention discloses a method for detecting arc faults based on the time-frequency characteristics of high-frequency current component. The method comprises the following steps: measuring the transient high-frequency current in a primary loop by using a self-integrating Rogowski coil and performing the spectrum analysis of the high-frequency current; measuring the time difference between the zero-crossing time of a power-frequency current and the time that the signal of the transient high-frequency current appears by adopting a counting method via using an interrupt trigger and a timer / counter, so as to confirm the phase angle position where the high-frequency current occurs in the protected loop load current; and judging whether an arc fault is caused or not according to the spectrum characteristics of the high-frequency current, the phase angle position where the high-frequency current signal occurs and regularity of the signal of the high-frequency current. The invention is capable of detecting arc faults in the normal power supply, is limited by installation position slightly, can distinguish the transient current generated by normally connecting / disconnecting a circuit from the power electronic-load current of the switch power supply and the like, and reduces and avoids misoperation.

The invention relates to a kit for determining heart-type fatty acid binding protein in serum or urine by latex enhanced turbidimetric immunoassay. Specifically, the kit for determining the heart-type fatty acid binding protein comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 contains a reaction promoter, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; the reagent R2 contains latex particles with binding of anti-heart-type fatty acid binding protein monoclonal antibody and polyclonal antibody, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; and the calibrator contains an antiseptic, an electrolyte, a stabilizing agent, a heart-type fatty acid binding protein pure product and a buffer. By the complex coating method of latex particles with the monoclonal antibody and the polyclonal antibody, high sensitivity and wide linear range of the kit are guaranteed. Simultaneously, the kit also has advantages of high accuracy, good repeatability, strong singularity, easy operation and the like, and is applicable to an automatic biochemical analyzer which is commonly used in clinic.

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.

The invention discloses a needle amperometric determination type glucose sensor for subcutaneous tissue real-time monitoring and manufacturing method thereof. The sensor comprises a needle reference electrode (2) and at least one needle working electrode (1) including a conducting layer (8) and an enzyme membrane layer (6), and is characterized in that the working electrode (1) further comprises a polymer material inner membrane layer (7) and a polymer material control diffusion layer (5), and the conducting layer (8), the polymer material inner membrane layer (7), the enzyme membrane layer (6) and the polymer material control diffusion layer (5) are coated in turn from inner to outside. According to the manufacturing method in the invention, the glucose sensor has good stability, high flexibility, wide linear range of output current and glucose concentration, short response time, continuous real-time monitoring, good fastness and stability of enzyme bonding, high flexibility, selectivity and repeatability.

The invention relates to composite aerogel of graphene / Prussian-blue complexes, and a preparation method and application thereof. The composite aerogel is in a three-dimensional network structure formed by graphene sheets. Particles of the Prussian-blue complexes are nano-scaled and attach to the graphene sheets. The preparation method of the composite aerogel includes adding metallic cyanide in graphene oxide suspension, allowing for dispersion to obtain suspension A, dissolving metal salt in aqueous solution containing reductant to obtain solution B, ultrasonically mixing the suspension A and the solution B, allowing for standing, and drying to obtain the composite aerogel. The composite aerogel is large in specific surface area and low in density and is highly conductive. The preparation method of the composite aerogel is simple to operate, low in cost and applicable to large-scale industrial production. The composite aerogel has excellent hydrogen storage performance, hydrogen peroxide catalytic reduction and electrochromic property.

The invention discloses a quantum dot multicolor marking method for quantitative detection of various cardiovascular disease markers and a kit of troponin I / creatine kinase isoenzyme / myohemoglobin. The method realizes fluorescent quantitative detection by utilizing excellent fluorescent properties of quantum dots and combining a multicolour marking technology and an immunochromatographic assay on the basis of optimizing each component of a test strip. Compared with the common collaurum immunochromatographic assay, the method has the advantages of good mark stability, low nonspecificity, high sensitivity, wide linear range, small cross interference, and accuracy in quantification. The kit disclosed by the invention is used for carrying out quantification detection on the troponin I, the creatine kinase isoenzyme and the myohemoglobin simultaneously, is suitable for detection of whole blood, blood serum and plasma samples, can provide a reference for cardiovascular and cerebrovascular disease diagnosis, and is widely applied to primary hospitals and clinics.

The invention discloses a method for detecting aflatoxin through an immunosensor. The aflatoxin (AFT) is a fungal toxin which has the high carcinogenicity, teratogenicity and toxicity, and accurate and rapid analysis on the aflatoxin is one of most effective means for reducing and avoiding harm of the aflatoxin. A prepared gold-polyaniline-graphene (Au-PANI-GN) composite nanomaterial is represented by adopting an ultraviolet spectrum and a transmission electron microscope and used for constructing the electrochemical immunosensor, the electrochemical immunosensor is represented by adopting a cyclic voltammetry method, an electrochemical AC impedance method and a square wave voltammetry method, and the prepared electrochemical immunosensor detects the aflatorxin B1. Experiment results show that the constructed electrochemical immunosensor has the good electrochemical activity on detection of the aflatoxin, and the concentrations of the aflatoxin and peak currents have the good linear relation, wherein the linear equation of the relation is y=-0.177x+4.28.

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

The invention discloses a compound color super resolution differential confocal measuring device and comprises a first super resolution differential confocal measuring device, a second super resolution differential confocal measuring device, a dichroic mirror, a partial color difference correction objective lens as well as a computer device. According to the chromatic dispersion characteristics that the focusing of optical beams with different wavelength produces an ideal translation of focusing surface, the device forms two partly overlapped linear measuring areas and achieves two linear measuring areas with the characteristics of double-polar tracking, and therefore, not only measuring range expands for nearly one times, but also cross tracking measurement of the measuring area is realized; according to the application requirement, unanimous or inconsistent transverse or axial scanning characteristics can be achieved respectively in the two linear measuring areas with independent modulation of a multi-super resolution filter, moreover, on the condition of remaining the advantages of the differential and super resolution differential confocal measuring technology, the invention expands the linear range of the measuring device significantly.

The invention discloses a kit for detecting alpha 1-microglobulin and a preparation method of the kit. The kit consists of a reagent R1 and a reagent R2 according to the volume ratio of 1: 1. The reagent R1 comprises 120-150 mmol / L of a buffer solution, 3-20 g / L of a stabilizer, 5-20 g / L of electrolyte, 3-20 g / L of a sensitizer, 0.5-10 g / L of a surfactant, 0.2-1 g / L of a preservative and purified water. The reagent R2 comprises 1.5-3.5 g / L of latex microspheres with two grain sizes, 20-150 mmol / L of a suffer solution, 3-20 g / L of a stabilizer, 5-20 g / L of electrolyte, 0.5 g / L of a preservative and purified water, wherein the latex microspheres with two grain sizes are respectively coupled with the same anti-human alpha 1-microglobulin polyclonal antibody. The pH value of the kit is 7.0-9.5. The kit is rapid in detection and high in accuracy. The production cost is greatly reduced. The kit is suitable for industrial production and has high application value.

The invention relates to the technical field of microfluidic chip chemiluminescent immunoassay, in particular to a chemiluminescent detection microfluidic chip and a chemiluminescent detection microfluidic chip system and application thereof. The chemiluminescent detection microfluidic chip comprises a sample feeding unit (1), a liquid storage unit (2), a reaction unit (3) and a waste liquid unit(4), and further comprises a quantifying unit (5) for quantifying a sample to be detected, wherein the quantifying unit (5) comprises a liquid inlet (51) connected with the sample feeding unit, a liquid outlet (52) connected with the waste liquid unit, a quantifying structure (53) for quantifying, and a temporary storage structure (54) for temporarily storing excess liquid, which is connected withthe reaction unit. The chemiluminescent detection microfluidic chip system consists of three layers, namely an upper layer, a middle layer and a lower layer; the middle layer is the chip; the upper layer and the lower layer are used for closing the middle layer; the upper layer is provided with a sample feeding hole connected with the sample feeding unit and a yielding hole corresponding to the liquid storage unit. The chemiluminescent detection microfluidic chip is simple in detection process, can quantify the reaction sample and has high sensitivity and strong repeatability.

The invention relates to a doped nano sensitive material for monitoring benzene, which is characterized in that: the doped nano sensitive material consists of Ag atom-doped TiO2, Fe2O3 and Al2O3 powder, wherein the content ranges of the components are that: the content range of Ag is 10 to 12 percent, the content range of TiO2 is 30 to 40 percent, the content range of Fe2O3 is 25 to 35 percent, and the content range of Al2O3 is 15 to 25 percent; and the grain diameter is less than 30nm. Benzene sensors prepared from the sensitive material provided by the invention have wide linear range, goodselectivity and high sensitivity, can monitor micro benzene in the air on line, and are not influenced by coexisting substances.

An apparatus for checking diametral dimensions of crankpins and main journals in the course of grinding is provided. The apparatus includes a head with a Vee-shaped reference device and an axially movable feeler that contacts the surface of the pin to be checked, and an articulated support device, coupled to the grinding wheel slide that carries the head and enables it to keep contact with the surface of the pin during possible orbital rotations. A transducer in the head cooperates with the feeler for detecting its displacements and transmits signals to a processing and display device in turn connected with the computer numerical control of the grinding machine. The processing and display device processes the transducer signals with compensation values or coefficients stored in a memory unit for obtaining measuring signals output by the head that vary in a linear way in a wide range and for compensating errors due to temperature variations.

The invention relates to a three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode as well as preparation and application thereof. The electrode consists of a three-dimensional Pt-Pb nano floricome array on a stainless steel acupuncture needle substrate, wherein three-dimensional Pt-Pb nano floricomes exist on the surface of the electrode in a good crystallization form, and the diameter of the single nano floricome is 50-80nm. The preparation method comprises the following steps of: polishing a stainless steel acupuncture needle on deerskin; after the stainless steel acupuncture needle is repeatedly washed through secondary distilled water and processed through ultrasound, putting the stainless steel acupuncture needle in a PBS (Phosphate Buffer Solution) of whichthe pH is 7.0 to scan and activate for 10 circles through cyclic voltammetry; putting the stainless steel acupuncture needle into a hydrochloric acid electrolyte of 0.5mol / L, which consists of chloroplatinic acid and lead acetate by a ratio of 1:1; and preparing an electrode sample through an ultrasonic oscillation electro-deposition method. The three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode has good electro-catalysis activity, wide linear response range and high sensitivity and selectivity.

The invention relates to a magnetic particle chemiluminescence double-layer micro-fluidic chip used for whole-blood sample detection. The micro-fluidic chip comprises a top plate (1) and a bottom plate (2). The top plate comprises a sample feeding port (4), a sample filling region (12), a marked ligand storage pool (5) and a sample mixing region (13). The bottom plate comprises a filtering region (6), a magnetic particle coating region (7), a cleaning region (14), a detecting region (8), a cleaning fluid storage pool (9), a luminous substrate liquid storage pool (10) and a liquid release channel (16).

The invention discloses a kit for detecting the content of neutrophil gelatinase-associated lipocalin (NGAL). The kit comprises a reagent R1, a reagent R2 and an NGAL reagent reference standard substance, wherein the reagent R1 comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA (Bovine Serum Albumin), 0.01%-0.1% of Tween-20, 0.5%-3% of PEG (Polyethylene Glycol) and 0.1% of NaN3; the reagent R2 comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA, 0.01%-0.1% of Tween-20, 0.1% of NaN3, 1%-10% of saccharose and 0.1%-1% of NGAL antibody sensitization latex particles; the NGAL reagent reference standard substance comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA, 0.01%-0.1% of Tween-20, 0.5mM-5mM of EDTA (ethylenediamine tetraacetic acid) and 0.1% of NaN3. The kit is simple in reagent composition, high in test sensitivity, wide in linear range, high in stability, low in test cost, high in accuracy and convenient to popularize.

System, method, and test strip for solid phase, electrochemical, quantitative analysis of analytes contained in biological fluid samples. Preliminary to analysis, a test sample solution can be applied to a sample collection pad associated with the solid phase test environment of the test strip. The test sample solution and a test kit reagent are thereby initially contacted, under assay conditions, within this solid phase test environment, and caused to migrate along a fluid pathway therein. Irrespective of the assay format (competitive assay, sandwich assay, etc.), a test kit reagent (e.g. labeled substance) and the analyte of interest (e.g. proteins, hormones or enzymes, small molecules, polysaccharides, antibodies, nucleic acids, drugs, toxins, viruses or virus particles, portions of a cell wall and other compounds which have specific or characteristic markers that permit their identification), either interact with one another to form a complex, or, alternatively, compete with one another for interaction with another test kit reagent, resulting in the concentration of an indicator substance within a delimited area of the solid phase. Thereafter, the delimited area of the test strip is subjected to electrochemical analysis and the results determined by monitoring an electrochemical transition in the form of an indicator, or derivative of the indicator (e.g. indicator species), by potentiostatic or potentiometric quantitative analysis (e.g. anodic stripping voltammetry). This electrochemical transition of the indicator has a characteristic electrical fingerprint that can be measured and which, when compared to a standard, can be correlated with the concentration of the analyte in the sample. This method is suitable for the determination / monitoring of therapeutic range of drugs (anti-convulsants drugs), determination of critical and potentially dangerous levels of endogenous materials which are indicative of disease states (prostate cancer), and numerous other applications presently requiring elaborate and time-consuming clinical laboratory analysis.

The invention discloses a fluorescent immunochromatography method for whole quantitative detection of C-reactive protein and a reagent kit thereof. The fluorescent immunochromatography method for the whole quantitative detection of the C-reactive protein (CRP) utilizes excellent fluorescent characteristics of quantum dots, and combines double-color marking technology and immunochromatography technology to achieve fluorescent quantitative detection on the basis of optimizing each constituent elements of test paper. Compared with a conventional colloidal gold immunochromatography method, the fluorescent immunochromatography method for the whole quantitative detection of the CRP has the advantages of being good in stability, low in non-specificity, high in flexibility, wide in linear range and accurate in quantifying. The reagent kit of the fluorescent immunochromatography method can perform the whole quantifying and can simultaneously predict and evaluate infectious diseases, antibiotic effects and cardiovascular and cerebrovascular diseases. The fluorescent immunochromatography method for the whole quantitative detection of the CRP and the reagent kit of the fluorescent immunochromatography method are suitable for various-level hospitals, and particularly contribute to wide popularization in basic-level hospitals and clinics.

The invention relates to a magnetic particle direct chemiluminiscence microfluidic chip for whole blood sample testing. The chip is composed of a top adhesive tape (10), a chip substrate (1) and a bottom adhesive tape (13); the chip substrate comprises a filtering area (2), a coating area (3), a cleaning area (4), a testing area (5), a cleaning liquid storing pool (7), a luminescence substrate liquid storing pool (8) and a waste liquid pool (11), wherein the testing area is connected with the cleaning liquid storing pool and the luminescence substrate liquid storing pool through liquid releasing channels (9) respectively; the top adhesive tape comprises a sample feeding opening (11) and a cleaning liquid storing pool demising hole (12).

The invention relates to an optimized cesium-light-pump weak magnetic detection device. In the optimized cesium-light-pump weak magnetic detection device, a non-magnetic-effect thermostatic controller enables a cesium spectrum lamp and a cesium sample absorbing bulb to be in the constant-temperature steam state, a radio frequency source stimulates the cesium spectrum lamp to emit light, and the light is converted into left handed circular polarization light to illuminate the cesium sample absorbing bulb, and focused on a photoelectric detector through a convex lens; a magnetoresistive sensor magnetic measuring module assists in measuring the value of a geomagnetic field of the position where the device is located, the range of strength is judged through an embedded type processing terminal module, and a self-oscillation module is controlled in a segmentation mode; photomagnetic resonance signals of the cesium sample absorbing bulb are detected by a low-noise photoelectric conversion circuit and simulate a high-frequency coil on the cesium sample absorbing bulb after being amplified and dephased so as to constitute a self-oscillation circuit, and Larmor signals are output; a Larmor signal measuring module and a big-dipper / GPS locating module are used for accurately measuring the value of the geomagnetic field of the position where the device is located. According to the optimized cesium-light-pump weak magnetic detection device, by means of the method achieving auxiliary magnetic field measurement, subband control, accurate frequency measurement and dual-mode locating, detection accuracy and reliability of the device are guaranteed.

The invention provides a preparation method and an application of a co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The preparation method comprises steps of: immobilizing tyrosinase and peroxidase on oxidized grephene to obtain a co-immobilized enzyme liquid and smearing dropwisely the co-immobilized enzyme liquid and a chitosan solution successively on a surface of a glassy carbon electrode to obtain the co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The electrode is low in cost, is easy and convenient to operate, is quick and sensitive, is low in detection limit, is good in selectivity and can be used for detecting microscale p-dihydroxybenzene and o-dihydroxybenzene in environment.

The invention relates to an immunochromatographic test strip for semi-quantitatively and simultaneously detecting cTnI and Myo and a preparation method thereof. The test strip comprises a base plate, a nitrocellulose membrane, a first bonding pad, a second bonding pad, a sample pad and a water absorbing pad. According to the preparation method, the pretreated nitrocellulose membrane, first bonding pad, second bonding pad, sample pad and water absorbing pad are sequentially and mutually staggered and attached to the base plate so as to obtain the immunochromatographic test strip for simultaneously detecting the cTnI and the Myo. The immunochromatographic test strip disclosed by the invention can be used for simultaneously detecting two proteins with greater abundance difference, providing great convenience for quick diagnosis of clinical myocardial infarction and overcoming and making up the defects of low sensitiveness and narrow linear range during detection of polyprotein in a traditional immunochromatographic test strip technology. The preparation method has the advantages of simple process, low cost, simpleness and convenience in operation and favorable application prospect.

The analytical process utilized in the system of this invention comprises three (3) step distinct steps wherein an analyte of interest in a test sample is initially labeled and subsequently isolated within a porous medium of a test device. Once isolated within the medium, the label is displaced from the analyte, or from the complex with the analyte, and converted, under electrolytic condition, to a metallic species which is caused test to deposit upon a working electrode of the test device. This working electrode is part of an electrode array that is positioned coincident with the porous medium, yet maintained physically remote therefrom. This deposit is then stripped from the working electrode, under anodic stripping conditions, and the current generated within the electrode array monitored. The characteristic response curve that is produced thereby can be correlated with the identity and concentration of the analyte(s) with the test sample.

The present invention provides a human thyroglobulin antibody magnetic separation enzymatic chemiluminescent immunoassay method, belonging to the field of immunodetection and analysis technology. Said method includes the following steps: making antigen human thyroglobulin be connected with Fe3O4 mincrosphere surface as solid phase reagent, the diameter of Fe3O4 microsphere is 0.1-5.0 microns, after the self-body antibody human thyroglobulin antibody being in the sample is trapped and two antibodies are labeled by enzyme reagent, the solid phase-antigen-antibody-enzyme-labeled two-antibody sandwiched immune complex can be formed.

The invention discloses a magnetic particle chemiluminescence micro-fluidic chip used for whole-blood sample detection. The chip is composed of top adhesive tape (12), a chip substrate (1) and bottom adhesive tape (15), wherein a filtering area (2), a coating area (3), a reacting area (5), a cleaning area (6), a detecting area (7) and liquid release channels (8) on the chip substrate are sequentially connected, a marking ligand storage pool (4) on the chip substrate is connected with the reacting area (5), the detecting area (7) is connected with a cleaning liquid storage pool (9) and a light-emitting substrate liquid storage pool (10) through the liquid release channels (8), and the top adhesive tape comprises a sample adding port (13). Magnetic particle marking ligands are coated with the coating area (3). The marking ligand storage pool (4) is used for storing enzyme or light-emitting agent marking ligands.

The invention discloses a method for applying an ionic liquid functionalized graphene modified electrode in concurrent detection of 5-hydroxytryptamine and dopamine, wherein an amine terminated imidazole ionic liquid and the epoxy group of graphene oxide lamellas are subjected to a nucleophilic ring opening reaction to form ionic liquid functionalized graphene, and then the ionic liquid functionalized graphene is modified on a glassy carbon electrode to form a modification layer so as to concurrently detecting 5-hydroxytryptamine and dopamine. According to the present invention, research results show that the ionic liquid functionalized graphene modified electrode provides good electrocatalysis activity for oxidation of 5-hydroxytryptamine and dopamine, and can be provided for individually detecting 5-hydroxytryptamine and dopamine and concurrently detecting 5-hydroxytryptamine and dopamine, and the method has characteristics of simple detection process, high sensitivity, rapidness and convenience.

The invention discloses a design and manufacture technology of a sensing unit of a sensor and one or more types of sensor chips consisting of the sensing unit, in particular to one or more types of sensor chips capable of detecting the magnetic field parallel and vertical to the surface of the chip and the acceleration of an object. The invention has the advantages that the process is simple and easy, the sensitivity is high, and batched production is easily realized; the sensing unit of the sensor comprises a wafer substrate, a seed layer, a soft magnetic material layer, a conducting layer and a N-layer structure; the conducting layer is wrapped by the soft magnetic material layer; the N-layer structure is formed by combination of the soft magnetic material layer and the conducting layer; and simultaneously a bent structure is formed by N long lines in the plane (the sensing unit is a single-strip-shaped structure when N is equal to 1). The sensing unit of the sensor realizes single-axis, double-axis and three-axis magnetic field detection by different packaging forms. Simultaneously, due to the combination of the sensing unit of the sensor and a cantilever structure with magnetic mass blocks, the single-axis, double-axis and three-axis acceleration detection can be realized.