79 results about "Environmental Estrogen" patented technology

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

The invention relates to a preparation method and application of an environmental estrogen electrochemical immunosensor, belonging to the technical field of electrochemical detection. The sensitivity of the sensor is obviously improved by adopting the characteristics that the conductivity of chitosan dispersed graphene is high, the stability is high, the specific surface area of a gold hybrid mesoporous silicon dioxide nano composite material is large, the biocompatibility is good, the catalytic efficiency is high and the like. The immunosensor is constructed by adopting the method of layer-by-layer self-assembly of the chitosan dispersed graphene, cross-linking agent, composite solution of nano materials and antibodies, and bovine serum albumin. In combination with specificities of antigens and antibodies, by virtue of an electrochemical workstation instrument, by recording current change before and after corresponding antigens modify the sensor, the environmental estrogens in water and food can be directly detected. The environmental estrogen electrochemical immunosensor prepared by adopting the preparation method has the advantages that the sensitivity is high, the specificity is good, the operation is easy to conduct and the detection limit is low, and the sensitive, rapid and accurate detection of various environmental estrogens in actual samples can be realized.

The invention provides a method for degrading environmental estrogen pollutants in a water body. The method comprises the steps of adding nascent state hydration manganese dioxide colloid into water containing estrogen pollutants with a concentration of between 0.1 and 10 mu mol / l, controlling a concentration of the manganese dioxide in the system of between 0.25 and 25 mu mol / l, a PH value of between 4.0 and 7.0 and a temperature of between 5 and 35 DEG C and stirring or oscillating the system solution with a rotating speed of between 100 and 150 rmp to degrade the environmental estrogen pollutants in the water body. The method is low in treatment cost, free of the restriction of field conditions, high in degradation efficiency, uneasy to generate secondary pollution, applicable to the degradation and removal of environmental estrogen pollutants in various natural water bodies and man-made water bodies, possessing a good industrialization promotion prospect.

The invention provides a preparing method of a functional magnetic MOFs (Metal-Organic Frameworks) material. A carboxyl or amino modified magnetic MOFs material is used as the functional magnetic MOFs material. The preparing method comprises the following steps: first, carboxyl or amino functional magnetic particles are prepared, and then, the functional magnetic MOFs material is prepared. The preparing method has the advantages that the preparing method of the functional magnetic MOFs material is simple in technology and easy to implement, the prepared functional magnetic MOFs material is used for extracting magnetic solid phases from estrogens, the regulation of the hydrophobicity, hydrophilicity and polarity of the magnetic MOFs surface is realized through the functional modification of the surfaces of Fe3O4 particles so as to realize the regulation and improvement of the absorption performance of the magnetic MOFs material, polluted trace environmental estrogens in environmental water can be efficiently absorbed and enriched, and powerful technique support is provided for the rapid, sensitive and efficient detection of the trace estrogens in the environmental water.

The invention discloses a preparation method of an environmental estrogen label-free immunosensor and a use of the environmental estrogen label-free immunosensor in environmental estrogen detection, and belongs to the technical field of novel functional nano-materials and environment detection. The preparation method utilizes the two novel functional nano-materials to modify working electrodes, utilizes synergetic catalysis effects and antibody sorption effects of the two novel functional nano-materials, and prepares the environmental estrogen label-free immunosensor which has high sensitivity, good specificity and fast detection rate, is prepared by simple processes and is used for environmental estrogen detection.

The invention discloses a preparation method of magnetic beads of dendritic molecular imprinting polymer on a surface of estrogenic nano silica gel. The preparation method comprises the following steps of: carrying out cross-over study on a magnetic nano material and dendritic polymer study technology, a dummy template technology and a surface molecular-imprinted technology and then carrying out dendritic molecular imprinting design of the surface of the magnetic nano silica gel on low-abundance environmental estrogens; by means of the advantage of nano construction with rich functional groups provided by the dendritic molecules, preparing a controllable, ordered and dense molecular imprinting nuclear shell on the surface of a carrier material and synthesizing a new molecular imprinting material with high efficiency and strong magnetism. According to the preparation method, the problem of low efficiency of surface molecular imprinting is solved and the fast and sensitive detection of residual estrogens in foods and environments is realized.

The invention discloses a derivatization method of environmental estrogens (estrone, 17beta-estradiol, estriol, 17alpha-ethynylestradiol), which is a treatment method and a core technology before a gas chromatography-mass spectrum (GC-MS) is used for analyzing the samples of the substances. The method comprises the following steps: firstly, leading high-purity nitrogen in at a room temperature to dry a standard mixed solution containing environmental estrogens; adding derivative reagents N, O-BSTFA and pyridine to react at room temperature (20 DEG C)-50 DEG C to obtain derivative products; drying; adding a sampling solvent and an internal standard; sampling and analyzing. The method has the advantages of simple and convenient experimental operation, time saving and low energy consumption and cost, simultaneously derivates the four environmental estrogens without generating side products, has favorable linear correlation among various derivative products and achieves an instrument detection limit up to 0.5pg / muL, thereby being applied to the GC-MS detection of environmental estrogens in water bodies, sediment, biological samples, and the like.

The invention belongs to the technical field of nanometer functional material, environmental monitoring, immunoassay and bio-sensing and provides a preparation method and an application of a two-channel environmental estrogen electrochemical immunosensor. The electrochemical immunosensor adsorbing metal ions and marked by aminated mesoporous ferroferric oxide is used for simultaneously detecting two environmental estrogens, and the electrochemical immunosensor has high sensitivity and effectively overcomes the defects of the existing environmental estrogen detecting methods, thereby having important scientific significance and application value on environmental monitoring.

The invention discloses a solid-phase micro-extraction capsule and application of the same in detection of phenol environmental estrogen in a beverage. A preparation method for the solid-phase micro-extraction capsule comprises the following steps: adding 4,4'-dipyridine and 1,2,3-benzenetricarbonic acid into a silver nitrate solution, carrying out a reaction at 150 to 170 DEG C for 2 to 4 d, and then successively carrying out cooling to room temperature, washing and drying so as to obtain a metal-organic nanotube material containing silver; and adding 0.5 to 1.5 mg of the prepared nanotube material into per 100 [mu]L of methanol, carrying out uniform dispersion, then injecting the obtained mixture into a hollow fiber film with a sealed end, then sealing the other end of the hollow fiber film and carrying out drying so as to obtain the solid-phase micro-extraction capsule. The invention also discloses a method for detecting phenol environmental estrogen in a beverage by using the solid-phase micro-extraction capsule. According to the invention, detection of multiple components of phenol environmental estrogen in the beverage is realized; and the method has the characteristics of rapidness, high efficiency, high sensitivity and the like and provides novel detection technology for quality safety monitoring of the beverage.

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

The invention belongs to the technical field of chemical analysis and test instruments, relates to the fields of polymer chemistry and analytical chemistry, and relates to a sol-gel ionic liquid solid phase microextraction extraction fiber and a preparation method thereof. In the preparation method, 1-allyl-3-methylimidazolium hexafluorophosphate or 1-allyl-3-methylimidazolium bis [(trifluoromethyl)sulfonyl] imide ionic liquid is taken as a coating material; and a layer of ionic liquid and hydroxy silicone oil composite coating is chemically bonded on the surface of a quartz fiber by a method for combining the sol-gel with free radical initiated crosslinking. The sol-gel ionic liquid solid phase microextraction extraction fiber prepared by the technical scheme can be directly combined with gas chromatogram, and has the advantages of porous surface structure, high thermal stability, high solvent washing resistance, wide pH application range, high repeatability, good extraction effect on strongly polar phenol environmental estrogen, arylamine and non-polar polycyclic aromatic hydrocarbon, and good application prospects in the fields of environmental monitoring, food sanitation, inspection and quarantine, and the like.

The invention relates to a test kit for qualitatively detecting fish vitellogenin by utilizing lipovitellin antibody. The test kit comprises pure product of goldfish lipovitellin and anti-rat goldfish lipovitellin polyclonal antibody. The preparation method of the test kit comprises the following steps: obtaining goldfish lipovitellin through purifying at first; then preparing anti-rat goldfish lipovitellin polyclonal antiserum through immune rats; and further purifying anti-rat goldfish lipovitellin polyclonal antibody. According to the invention, the test kit can be used for screening environmental estrogen materials and monitoring the situation of water environment which is polluted by the environmental estrogen materials, so that vitellogenin in fish blood, overall uniform serous fluid, skin mucus, liver tissues and hepatic cell nutrient solution can be monitored sensitively and conveniently. The test kit can be used for qualitatively detecting cyprinidae fish vitellogenin, and has the same monitoring sensitivity to the fish vitellogenin of a far provenance relationship fish. The biggest advantage of the test kit is that the test kit has a very wide application scope, and the minimum monitoring limit can reach 100ng / ml.

Pseudomonas fluorescens capable of degrading estrogen substances is named as SJTE-2, and a preservation number is CGMCC No.6587. The pseudomonas fluorescens can use natural estrogen or environmental estrogen as the only carbon source to grow and can degrade estrogen substance oestrone, 17-Beta-estradiol, estriol and polycyclic aromatic hydrocarbon substance naphthalene, luxuriant, bisphenol A, and can be used for decomposing and removing an estrogen substance and a polycyclic aromatic hydrocarbon substance in an environment. Under the culture condition that the estrogen or the polycyclic aromatic hydrocarbon is used as the only carbon source, a bacterial strain SJTE-2 can degrade oestrone, 17-beta-estradiol and estriol within 24 hours, wherein the initial concentration of the oestrone, 17-beta-estradiol and estriol is 1 mg / L, and degradation rate of naphthalene, luxuriant and bisphenol A is over 90%, wherein the concentration of the naphthalene, luxuriant and bisphenol A is 50 mg / L. The bacterial strain can degrade oestrone, 17-beta-estradiol and estriol for more than 90% in 7 days, wherein the initial concentration of oestrone, 17-beta-estradiol and estriol is 50 mg / L, and degradation rate of naphthalene, luxuriant and bisphenol A is larger than 80%, wherein the concentration of naphthalene, luxuriant and bisphenol A is 500 mg / L.

The invention discloses a preparation method of a solid phase extraction column of molecular imprinted of phenolic environmental estrogens. The method comprises steps of: uniformly mixing 100-500mg of molecular imprinted polymer of phenolic environmental estrogens with 5-20mL of pure organic solvent, and uniformly mixing 100-500mg of traditional solid phase extraction packing with 5-20mL of pure organic solvent; orderly moving the mixtures into a hollow solid phase extraction column; plugging two ends of the solid phase extraction column with sieve plates; adding an acid organic solvent into the solid phase extraction column to wash after the solid phase extraction column being uniformly filled; flushing the filled solid phase extraction column with the pure organic solvent; purging the filled solid phase extraction column with inert gases to remove residual organic solvent; and drying to obtain the product. The preparation method disclosed by the invention is simple, and has particularity matching with the molecular imprinted polymer of phenolic environmental estrogens; and the prepared solid phase extraction column performs high selectivity and high recovery rate to the phenolic environmental estrogens, and is capable of obviously reducing the matrix effects.

The invention relates to a co-detecting method for environmental estrogens in a surface water body, and aims at solving the problems that the treatment process time is long, a large quantity of samples and reagents are consumed in an off-line solid phase extraction method, the process is complicated, the interference factors are more, and loss is large. The co-detecting method is implemented through the following steps that 1, sample collecting and pretreating are performed; 2, filtering and enriching are performed, wherein a to-be-detected surface water body sample which is preserved after being pretreated are enriched and purified through an on-line solid phase extraction device column; 3, detecting and analyzing are performed, wherein a result obtained in the step 2 is detected by adopting an ultra-high performance liquid chromatography tandem mass spectrometer to obtain the detection limit and a quantitative and qualitative result of the seven environmental estrogens in the to-be-detected surface water body sample; 4, a standard curve is obtained; 5, the result obtained in the step 3 is compared with the standard curve to obtain the content and recovery rate of the environmental estrogens. The co-detecting method is applied to the field of surface water detection.

The invention discloses a method for detecting estrogen pollution of water on the basis of polymorphic vitellogenin of periophthalmus modestus, and belongs to the field of molecular biology and environment pollution detection. Amino acid sequences of the vitellogenin of the periophthalmus modestus are shown as SEQ ID NO.2. The method has the advantages that the problems of deficiency of biological in-vivo detection for environmental estrogen by the aid of periophthalmus modestus and deficiency of corresponding biological detection technologies at present can be solved; vivo models of the Periophthalmus modestus which is high in tidal flat settlement property and sensitive to environments are selected, primers are designed, expression of the vitellogenin VtgAa of the periophthalmus modestus is detected by the aid of real-time quantitative fluorescent PCR (polymerase chain reaction) processes, and the method can be used for detecting estrogen pollution degrees of water environments such as seawater and mouths of rivers.

The invention discloses a baby product TPE (Thermoplastic Elastomer) and a preparation method of the baby product TPE, and solves the problems that: adding a plasticizer in the TPE material causes baby allergies easily, and as the plasticizer contains phthalate ester in environmental estrogen and forms pseudo-hormone easily, the hormone content in the body is affected, the original mechanism of internal secretion is further disturbed and the endocrine dyscrasia of a baby is caused. The key point of the technical scheme is as follows: the material of the baby product TPE comprises the following components by mass: 20-40 parts of SEBS (Styrene Ethylene Butylene Styrene block copolymer), 50-60 parts of cosmetics grade white oil, 5-15 parts of shell powder, 5-15 parts of polyolefin, 5-10 parts of a compatilizer, 0.5-1.5 parts of a slipping agent, 0.1-0.3 part of an antioxygen, 0.1-0.2 part of an antiaging agent and 0.1-0.5 part of an antibacterial agent, no plasticizer is added, and the condition that the allergy of the baby skin is caused by the addition of the plasticizer is avoided.

The invention discloses a recombined human estrogen Alpha receptor ligand binding domain protein as well as a preparation method and application of the recombined protein. The recombined protein includes a segment from the 270th amino acid residue to any one of the 554th to 595th amino acid residue of the human estrogen Alpha receptor protein as well as a 6*His tag connected with an N end of the segment and a Strep tag II connected with a C end, and is superior than the already-reported congeneric proteins in the aspects of stability and binding activity with an estrogen compound. The recombined human estrogen Alpha receptor ligand binding domain protein is provided with the double tags, thereby facilitating purification and follow-up application. An affinity adsorbent for an estrogen receptor, which is prepared by immobilizing the recombined protein on a carrier surface, can be applicable for the selective purification and the enrichment of an environment estrogen pollutant and providing a powerful way for the high throughput detection of the environment pollutant.

The invention discloses a method for testing toxicity of environmental estrogen on whitebait embryonic development. The method of the invention utilizes the sensitivity of embryo to the simulation of environmental pollutant or chemical matter to perform a test under semi-static state on whitebait embryo which is exposed in standard diluted aqueous solution of subject having a series of concentrations. The test lasts about 30 days, begins as exposing a live embryo at the blastula stage in the aqueous solution of subject, and ends with hatching all embryos in a control group and an exposed group. The toxicity endpoint contains death, monstrosity and delayed hatching. The method comprises the steps of confirming the toxicity of the subject on the whitebait embryonic development by observing the toxicity endpoint of the exposed group and comparing with the control group; researching on the influence of two natural estrogens E2 and EE2 on the whitebait embryo-yolk sac stage so as to confirm the toxic dose-effect relationship of the natural estrogens to the whitebait embryonic development, and evaluating the potential risk of this type of fish exposed under the estrogen with environmental concentration.

The invention relates to a water estrogen pollution detection method based on oryzias melastigma vitellogenin, and belongs to the field of environmental pollution detection. Currently, no marine fish is used for environment estrogen biological detection, and biological detection technology of seawater estrogen pollution is insufficient. According to the invention, euryhalic male oryzias melastigma with strong adaptability is selected as an in-vivo model; a primer is designed; the expression of vitellogenin (VTG1) is detected by a real-time quantitative PCR method; and 18S ribosome RNA (18S) is used as reference genes to establish a detection system of the VTG1 transcriptional level. Male fish is exposed to 17-beta-estradiol and clean artificial seawater, which are used as a positive and a negative control of the system, and appropriate exposure time is explored. The detection method can be used for detection of environment estrogen pollution degrees of seawater samples, river estuary samples, and saltwater internal lake water samples.

The invention discloses a method for detecting trace phenols environmental estrogen and belongs to the technical field of biological samples and environment detection (monitoring). According to the method, the extraction comprises the following steps: adjusting the pH (Potential of Hydrogen) value of a solution to be detected to be 2.5-3.5; adding a surfactant and an extracting agent; uniformly mixing by ultrasonic waves; centrifuging at a low temperature; removing an upper layer; quantitatively detecting by adopting a high performance liquid chromatography. According to the method, an ultrasonic wave assisting surfactant emulsification and micro-extraction technology is adopted and the surfactant with low toxin is used for replacing toxic dispersants including acetone, methanol, acetonitrile and the like; the high performance liquid chromatography (HPLC) is combined to be used for quantitative detection; the method has the advantages of high efficiency, low toxin, simplicity and convenience in operation, low cost and the like; the enriching multiple is up to more than 200 times and the detection range is within 0.01 microgram / liter-0.1 microgram / liter; the method can be widely applied to detecting (monitoring) and analyzing the phenols environmental estrogen in spiral seaweed biological samples, foods and medicines.

The invention discloses recombinant yeast for detecting environmental estrogen and a constructing method and use of the recombinant yeast. The recombinant yeast comprises a yeast expression plasmid and a yeast report plasmid, wherein the yeast expression plasmid comprises a tanichthys albonubes estrogen receptor gene TERalpha represented by the sequence shown as SEQ ID No.1, and a carrier of the yeast report plasmid is pMP206, and is linked with a tanichthys albonubes vitellogenin gene promoter 2 represented by a sequence shown as SEQ ID No.4. The tanichthys albonubes estrogen receptor is used for constructing the expression plasmid, the tanichthys albonubes vitellogenin gene promoter is linked with the report gene to construct the report plasmid. The tanichthys albonubes is sensitive to the change of the living environment, and is a wonderful fish biological marker for monitoring the environmental estrogen pollution by using a biological method, and the vitellogenin is a good biological marker for the environmental internal-secretion interfering-substance. The estrogen receptor and the vitellogenin promoter of the tanichthys albonubes are used for constructing a recombinant yeast evaluation system, so that the sensitivity can be greatly improved.

This invention discloses environment organic pollution estrin based on molecule rapid filter method, which comprises the following steps: firstly establishing filter estrin module with aromatic nucleus compound along structure into agent eight compound, wherein, it uses Dragon software to compute structure information parameter and to sort them; using QASAR module to establish identification function and sort gravity; according to structure to judge type of organic compound; then using dragon software to compute relative structure parameter value to judge the results.

The invention relates to a hydrothermal carbon modified material capable of removing an environmental estrogen pollutant 17beta-estradiol, in particular to a hydrothermal carbon / attapulgite / beta-FeOOH composite material. A preparation method of the material comprises the following steps: performing hydrothermal carbonization on attapulgite and biomass; assembling the attapulgite on the surface of hydrothermal carbon; activating with KOH; loading beta-FeOOH onto the surface of the material; increasing the specific surface area to prepare a high-performance hydrothermal carbon / attapulgite / beta-FeOOH composite adsorption material. The modified hydrothermal carbon material prepared by the method has a large quantity of adsorption sites, and can be used for efficiently removing the environmental estrogen 17beta-estradiol. Moreover, the hydrothermal carbon modified material is easy to prepare, is low in cost, has high adsorption performance, and is an adsorbent with a wide market prospect.

The invention provides a gobrocypris rarus vitellogenin monoclonal antibody and application thereof. The gobrocypris rarus vitellogenin monoclonal antibody is secreted by a hybridoma cell line with a storing number: CGMCC No.2869. The hybridoma cell line can stably secrete the gobrocypris rarus lvitellogenin monoclonal antibody. A provided kit which comprises the monoclonal antibody can simply, accurately and sensitively detect the vitellogenin in gobrocypris rarus serum in a qualitative / quantitative mode. The Gobrocypris rarus vitellogenin monoclonal antibody is an analysis tool which can simply and effectively detect / evaluate environmental estrogen hormone effect and has wide application prospect.

The invention discloses a fabrication method of an environmental estrogen electrochemical light-emitting sensor, and belongs to the technical field of novel nanometer functional material and biological sensing analysis. A cobalt hydroxide nanosheet array is fabricated on a disposable throwable electrode, and an electronic medium-containing polydopamine thin film and a molecular imprinting polymertaking environmental estrogen as a template molecule are directly and sequentially fabricated on the cobalt hydroxide nanosheet array by an in-situ growth method according to large specific area, high-activity hydroxyl functional group and amide group of polydopamine. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecular imprinting molecule of the template molecule is eluted. Therefore, the environmental estrogen electrochemical light-emitting sensor is fabricated and completed.

The invention relates to a genetic engineering kit for detecting environmental estrogen pollutants and application thereof, and belongs to the technical field of detection. The kit comprises the following components in part by volume: 2*10<4> parts of LB culture medium, 1 part of isopropyl-beta-D-thiogalactoside, 20 parts of chloroform, 10 parts of solution of lauryl sodium sulfate, 20 parts of o-nitrophenyl-beta-D-galactoside and 100 parts of solution of sodium carbonate. A detection process of the genetic engineering kit is simple, convenient and quick, and results can be obtained within 2 to 3 hours; the genetic engineering kit does not have special requirements on instruments, demand quantity of samples is fewer, detection stability is high, and the detection sensitivity can reach n g / L; and the genetic engineering kit improves the analytical rate while reducing the analytical cost greatly.

The invention discloses a degradation method and evaluation method for cooperatively processing nine environmental estrogens.The degradation method includes the steps that a to-be-processed water sample is filtered through a 0.45-micron microfiltration membrane, a Ti / IrO2-Pt electrode serves as a positive pole, a Ti plate serves as an electrode plate of a negative pole, and the Ti / IrO2-Pt electrode and the Ti plate are inserted into the to-be-degraded water sample; a stirrer is put into the to-be-processed water sample, sodium sulfate serves as an auxiliary electrolyte, and sodium chloride and hydrogen peroxide are added; the stirrer is started, the to-be-processed water solution is in the evenly-stirred-mixed state, and a switch of a direct-current power source is turned on; hydrogen peroxide is added into the water sample subjected to electrochemical processing, and the mixture is further subjected to electro-beam irradiation processing.The evaluation method includes the steps of water sample pretreatment; solid phase extraction; derivatization; gas chromatograph-mass spectrometer detection; data processing.According to the degradation method and the evaluation method, under the effect of a direct current, the good degradation effect on the nine environmental estrogens is achieved, and the degradation method and the evaluation method are simple and easy to carry out, high in operability and low in cost.

The invention discloses application of a scatophagus argus vitellogenin gene to detection of water area environmental hormones. The application comprises the following steps: obtaining a VTGAb full-length sequence according to a RACE method; sampling and extracting liver RNA (Ribonucleic Acid) after injecting estrogen into scatophagus argus in a gradient manner; detecting expression of VTGAb by adopting an RT real-time quantitative PCR (Polymerase Chain Reaction) method; taking EF1-a as an internal control gene and carrying out absolute quantification on cDNA (complementary Deoxyribonucleic Acid) of liver tissues to establish a detection system of a VTGAb transcriptional level; putting scatophagus argus which is not sexually mature into a water body to be detected and sampling and extracting the liver RNA; detecting expression amount of the VTGAb; and comparing with the detection system, and analyzing the pollution degree of the environmental hormones of a water sample. By adopting the application disclosed by the invention, the pollution condition of the environmental hormones can be measured under the condition that the economic loss is relatively small and the single variable of the scatophagus argus is controlled; and the scatophagus argus vitellogenin gene can be used for the pollution degree of the environmental hormones of water samples of seawater, the mouth of a river and a saline water internal lake.

The invention discloses a recombined human estrogen Alpha receptor ligand binding domain protein as well as a preparation method and application of the recombined protein. The recombined protein includes a segment from the 270th amino acid residue to any one of the 554th to 595th amino acid residue of the human estrogen Alpha receptor protein as well as a 6*His tag connected with an N end of the segment and a Strep tag II connected with a C end, and is superior than the already-reported congeneric proteins in the aspects of stability and binding activity with an estrogen compound. The recombined human estrogen Alpha receptor ligand binding domain protein is provided with the double tags, thereby facilitating purification and follow-up application. An affinity adsorbent for an estrogen receptor, which is prepared by immobilizing the recombined protein on a carrier surface, can be applicable for the selective purification and the enrichment of an environment estrogen pollutant and providing a powerful way for the high throughput detection of the environment pollutant.