262 results about "Functional modification" patented technology

The present invention provides a high-selection hydrogen permeation separation performance metal organic framework membrane for efficient preparation, and especially relates to a zeolite imidazole organic framework membrane, and preparation method of ZIF-8 membrane and a metal organic framework membrane prepared by the method are provided. The method comprises the following steps: using dopamine for functional modification of a solid carrier; and synthesizing the metal organic framework membrane on the surface of the functionally modified solid carrier. By preparation of the metal organic framework membrane by the method, nucleation and growth are greatly enhanced, the prepared metal organic framework membrane is homogeneous and dense, falling-off condition after membrane formation can be significantly improved, and gas separation performance is good; the method has the advantages of mild operation condition, easy industrialized amplification, environment friendliness, and strong applicability. The method can also adopts microwave heating method, the reaction time can be shortened, and the cost of industrial production can be further reduced.

The invention discloses a preparation method and an application of a nano material monolithic column immobilized enzyme biological micro-reactor. The preparation method comprises the following steps of firstly, preparing a porous organic polymer monolithic column by using a mixed solution of a functional monomer, a crosslinking agent, a pore forming agent and an initiating agent through in-situ thermal-initiated or light-initiated polymerization in the column, and then bonding a nano material after functional modification to obtain a nano material monolithic column; and secondly, realizing immobilization of the enzyme on the monolithic column by using the nano material as an intermediate ligand to obtain the nano material monolithic column immobilized enzyme biological micro-reactor. The biological micro-reactor is successfully applied to proteomic analysis, medicament chiral resolution and catalyzed ester exchange reactions. The biological micro-reactor disclosed by the invention has the following advantages that the preparation method is simple, the immobilization amount of the enzyme is large, the catalytic activity is high, the enzymolysis speed is high, the efficiency is high, the service life is long, and the biological micro-reactor can be reused.

The invention discloses a preparation method of a magnetic antiphase demulsifier. The preparation method is characterized by firstly, preparing monodisperse ferroferric oxide magnetic microspheres by a hydrothermal method; secondly, coating a layer of thin silicon dioxide on the surfaces of the monodisperse ferroferric oxide magnetic microspheres, synthesizing magnetic microspheres with nuclear shell structures, performing group functional modification on the nuclear shell magnetic microspheres by using a silane coupling agent KH560; at last, performing grafting reaction by using the activity of functional groups and an antiphase demulsifier to obtain the magnetic antiphase demulsifier; the magnetic antiphase demulsifier prepared by the preparation method can improve the efficiency of original antiphase demulsifiers and has the characteristics of easy separation, high oil removing rate, obvious separation effect to oil-containing sewage, little use amount and high efficiency.

The invention discloses a carrier for immobilization as well as a preparation method thereof and immobilized beta-glucosaccharase. The carrier for the immobilization comprises magnetic nano particles, a carboxyl-rich composite material and transitional metal ions, wherein the carboxyl-rich composite material wraps the surface of the magnetic nano particles to form a carboxyl-rich shell, carboxyl on the surface is complexed with the transitional metal ions, and the transitional metal ions are combined with to-be-immobilized enzyme through a metal ion coordination bond. The preparation method comprises the following steps of (1) preparing the magnetic nano particles; (2) carrying out hydroxyl functional modification for the particles; (3) carrying out epoxy functional modification for the particles; (4) carrying out carboxyl functional modification for the particles; (5) complexing the particles with the transitional metal ions. The functional carrier is combined with beta-glucosaccharase through the metal coordination bond to obtain immobilized beta-glucosaccharase. The biological enzyme is firm in combination, high in enzyme activity, strong in operation stability and suitable for the mass production.

The invention discloses a preparation method and application of a three-dimensional graphene electrode for an electrochemical biosensor. The preparation method comprises the following steps of: fixing spongy graphene in which industrially produced foam nickel is taken as a substrate and which has a three-dimensional structure and is synthesized through chemical vapor deposition on a glass or quartz sheet; connecting the spongy graphene with the three-dimensional structure and a wire by using a silver conductive adhesive; and coating organic silica gel on a connection point of the metal wire and the graphene for insulation to obtain a spongy graphene electrochemical electrode with the three-dimensional structure. The three-dimensional spongy graphene electrode has the outstanding characteristics of high conductivity, high specific surface area, high electrochemical stability and the like, is easily subjected to surface functional modification, and has high detection sensitivity to dopamine and nicotinamide adenine dinucleotide; and a highly sensitive electrochemical biosensor for non-enzymatically and selectively detecting glucose can be obtained after the surface of the electrode is modified by Co3O4.

The invention relates to a high strength chitosan complex fiber which has a mold resistance and is applicable to medical treatment and clothing textile fabric, and a preparing method thereof. The chitosan complex fiber obtained according to the technical scheme has a much higher overall performance than the products obtained through the prior art, has a good spinning property and good stitchability, and can be further prepared into fabrics through a knitting machine or a weaving machine as well as non-woven fabrics through a nonwoven equipment. The adopted oxidized graphene in itself has a good antibiosis effect, and compared with a graphene material, the oxidized graphene has the advantages of being easy to prepare, high in performance, low in cost, good in water dispersibility, good in adsorptive property, capable of easily conducting various kinds of functional modification and the like. Dosage adjustment and chemical modification can maintain superiority of the graphene and at the same time prevent the graphene from inducing biotoxicity so that the research and application of the graphene in the field of biomedical engineering can be broadened. The preparing method of the high strength chitosan complex fiber has the advantages of being simple in producing technology, low in preparing cost; meanwhile, the obtained high strength chitosan complex fiber has a stable performance. Therefore, the preparing method is very suitable for popularization and market expanding of the chitosan fiber and graphene industries.

The invention discloses a biological enzyme process antibacterial and anti-wrinkle finishing method of a silk fabric, belongs to the field of a textile biotechnology, and aims to overcome the defects of high baking temperature, high probability of damage of silk fibers and reduced whiteness in real silk antibacterial and anti-wrinkle treatment of the conventional chemical method and realize enzyme-promoted antibacterial and anti-wrinkle functional modification of a real silk fabric. According to the method, a chitosan natural finishing agent with a primary amine structure is grafted to the surface of the silk fiber by utilizing the catalytic oxidation effect of tyrosinase, so that the antibacterial and anti-wrinkle effects of the silk fabric are improved. The process flow comprises the following steps of: pre-treating the silk fabric; catalyzing grafting modification treatment of chitosan by the tyrosinase; washing; and drying. Through the silk fabric treated by the invention, the antibacterial performance is improved, the wrinkle recovery performance is improved, and the mechanical property and the dyeing performance of the fabric are also improved. Compared with the conventional chemical method, the method has the advantages of adoption of the tyrosinase process for catalysis of the antibacterial and anti-wrinkle treatment process of the real silk, low energy consumption, high efficiency, less pollution and contribution to environmental protection.

The invention provides a preparing method of a functional magnetic MOFs (Metal-Organic Frameworks) material. A carboxyl or amino modified magnetic MOFs material is used as the functional magnetic MOFs material. The preparing method comprises the following steps: first, carboxyl or amino functional magnetic particles are prepared, and then, the functional magnetic MOFs material is prepared. The preparing method has the advantages that the preparing method of the functional magnetic MOFs material is simple in technology and easy to implement, the prepared functional magnetic MOFs material is used for extracting magnetic solid phases from estrogens, the regulation of the hydrophobicity, hydrophilicity and polarity of the magnetic MOFs surface is realized through the functional modification of the surfaces of Fe3O4 particles so as to realize the regulation and improvement of the absorption performance of the magnetic MOFs material, polluted trace environmental estrogens in environmental water can be efficiently absorbed and enriched, and powerful technique support is provided for the rapid, sensitive and efficient detection of the trace estrogens in the environmental water.

The invention relates to a mixed matrix type cation exchange membrane and a preparation method thereof. The cation exchange membrane takes a sulfonated modified polymer material as a substrate and isdoped with Schiff base type covalent organic framework materials loaded with different functional groups. The preparation method comprises the following steps of: sequentially performing monomer preparation and polymerization of a covalent organic framework material, sulfonation modification of a polymer, uniform mixing of the covalent organic framework material and a sulfonated polymer, and flow-spreading film formation; and finally, performing acid treatment to obtain the cation exchange membrane. The covalent organic framework material can effectively improve the vanadium resistance of themembrane based on the rigid porous structure; the organic porous structure of the covalent organic framework and the different functional modifications can partially compensate the loss of membrane proton conductivity caused by the introduction of particles; the rigid framework can obviously inhibit the swelling in the membrane and can improve the tensile strength of the membrane; COFs is composedof organic structures and can promote compatibility between sulfonated polymers and covalent organic framework materials; and moreover, the polymer as the substrate can improve the film forming performance of the covalent organic framework material, so that the covalent organic framework material has the better mechanical strength.

The invention relates to a functional improvement method of woods, in particular to a manufacturing method of a carbonization heat treatment-modified solid wood flooring, belonging to the technical field of functional modification of the woods and wood product processing. The technical solution for the manufacturing method comprises the following steps: (1) a solid wood flooring blank manufacturing step; (2) a drying step; (3) a carbonization heat-treatment improving step; and (4) a solid wood flooring manufacturing step. Due to the technical solution for the manufacturing method of a carbonization heat treatment-modified solid wood flooring, a production method of a solid wood flooring designed special for fast-growing woods while the existing technical problems are solved. Compared with the prior art, the manufacturing method has the advantages of simple production process, short production cycle, low energy consumption, low production cost and the like, and technical indexes of obtained solid wood flooring made of fast-growing woods achieve the national standard quality requirement.

The invention discloses a cross-linked hollow fiber anion exchange membrane and a preparation method thereof. The preparation method is characterized by comprising the steps that an aromatic polymer subjected to halomethylation is dissolved in an organic solvent, and a membrane casting solution is formed; the membrane casting solution is subjected to dry-wet spinning, a hollow fiber basement membrane is obtained, cross-linking and positively charged modification are conducted on the hollow fiber basement membrane through a one-step or two-step method, and the cross-linked hollow fiber anion exchange membrane is obtained finally. According to the cross-linked hollow fiber anion exchange membrane and the preparation method thereof, the basement membrane and a modified functional agent which are high in activity are selected, and by means of a nucleophilic substitution reaction between the basement membrane and the modified functional agent, functional modification to the basement membrane can be achieved under a very mild condition. The method has the advantages of being simple in process and low in cost, and meanwhile the prepared membrane has a good diffusion permeability property and has a large-scale application prospect in the field of acid recovery.

The invention discloses a multi-element functional modification polyvinyl alcohol-based lithium ion battery aqueous binder and application of the binder in an electrochemical energy storage device. According to the binder, polyvinyl alcohol is taken as substrate, and hydrophilic monomers and oleophylic monomers are taken as functional modification monomers. Through adoption of Michael addition reaction modification preparation, the binder has good water solubility, elasticity and bonding performance. Through application of the binder in the preparation process of the lithium ion battery electrode plate, the homogeneity of electrode slurry can be improved. Therefore, the electrode slurry can form film on a current collector evenly and flatly, the material of the electrode plate will not fall off, the capacity will not be reduced, the high rate capability and circulation stability of the lithium ion battery cathode and anode materials can be greatly improved, and the service life of the battery can be effectively extended.

The invention discloses a functional polymer containing phosphorylcholine and PEG and a method for forming a biological pollution resistant interface. The polymer is a functional polymer that contains multiple function groups on a side chain and has a controllable proportion; a PEG flexible chain with larger steric hindrance and outer cell membrane phosphorylcholine hydrophilic group interact to achieve good anti-pollution effect; the polymer contains less than 10% of catechol group, and can be strongly combined with surfaces of various polydopamine-mediated base materials to form a stable hydrophilic monomolecular coating, so that the functional modification of material surfaces is implemented. The method for forming coatings is simple; the coatings can exist on the surface of any base material, so that the ability of resisting adhesion and pollution of multiple biological ingredients of the material surface is improved.

The invention discloses a new synthesis method of a polyaniline / carboxylated graphene composite material and belongs to the technical field of composite materials. Firstly, functional modification is conducted on the surface of graphite oxide through NaOH and monochloroacetic acid to convert an epoxy group, a hydroxide group and other oxygen-containing groups on the surface of graphite oxide into carboxyl groups; secondly, graphite oxide is wrapped by a surfactant, and then the carboxyl groups are grafted onto the edge of graphite oxide together with diazonium salt, and carboxylated graphene is obtained; lastly, the carboxylated graphene and an aniline monomer are polymerized, and the polyaniline / carboxylated graphene composite material is prepared. According to the composite material, polyaniline is loaded on the surface of graphene and connected with graphene under the action of a covalent bond; the composite material has a large specific surface area, a novel structure and good electrochemical properties, mechanical properties and chemical stability, and can be used for preparing a supercapacitor, a selective absorbent for specific ions, a catalyst and the like.

The invention relates to a method for preparing functional modified hollow mesoporous or core / shell mesoporous SiO2 nanometer granules. The method comprises the following steps of: (1) synthesizing, namely mixing a silane coupling agent with long-chain functional groups and a silicon source uniformly, adding the mixture into an alkaline solution containing monodisperse SiO2 nanometer particles, and performing centrifugal separation on a reaction product to prepare solid core / shell mesoporous SiO2 nanometer spheres; and (2), dispersing the solid core / shell mesoporous SiO2 nanometer spheres prepared in the synthetic process in the alkaline solution, and performing hydro-thermal reaction to obtain the functional modified hollow mesoporous or core / shell mesoporous SiO2 nanometer granules. According to the method, the synthesis and functional modification of the hollow or core / shell mesoporous SiO2 nanometer granules can be implemented synchronously by the process. By the process for synthesizing the functional hollow or core / shell mesoporous SiO2 spheres 'synchronously', the blockage of pore channels in shells by a post modification method is avoided, and the process is simple and easy, pollution-free, high in yield, low in cost and high in efficiency.

The invention relates to a functional separator for a lithium sulfur battery, and a preparation method and application thereof, belonging to the technical field of electrochemistry. The functional separator of the invention is composed of a polymer separator matrix and a functional modification layer applied one side surface of the polymer separator matrix, wherein the functional modification layer comprises a binder, a conductive carbon material and a dendritic branched macromolecule. The adhesive of the invention has good adhesion and high stability. The conductive carbon material has a veryhigh electron conduction rate, can improve the utilization rate of the active substance and greatly reduce the internal impedance of the battery. The dendritic branch macromolecule contains more organic functional groups, the dendritic branched macromolecule has chemisorption to the polysulfide generated in the sulfur positive electrode region during the cycle, and the carbon material has physical adsorption to the dissolve polysulfide, thereby effectively inhibiting the shuttle effect in the lithium-sulfur battery. Therefore, the lithium sulfur battery prepared using the functional separatordescribed in the present invention exhibits excellent cycle performance and rate performance.

The invention provides preparation and application of an electrochemical micro-fluidic device for nucleic acid isothermal amplification. A micro-fluidic electrochemical sensor device is directly established on a printed electrode. The device comprises two layers of PDMS chip. The micro-channels on the upper layer are used to fully mix various samples. The channel area in the lower layer is divided into a reaction area and a detection area. A temperature-control element is integrated to the device. The electrode surface is subjected to functional modification. Corresponding primers aiming at the hepatitis B virus nucleic acid are designed. Electrochemical reporter molecules are grafted onto the primers. The labeled primers and the nucleic acid to be detected are evenly mixed, then the mixture is injected into a micro-fluidic chip, and a proper temperature is controlled to carry out isothermal amplification reactions on the nucleic acid sample. The labeled primers are combined to the electrode surface through the base pairing effect during the amplification process. Then the virus can be detected through the detection on the reporter molecules. The provided device has the advantages of miniaturization, low cost, and portability, can perform rapid and high-sensitive detection, and is convenient for diagnosis in outdoors / home.

The invention provides a functional plant aromatic antibacterial cellulose fiber. The fiber comprises a microcapsule. The wall of the microcapsule is divided into an inner layer and an outer layer. The inner layer is made of polyacrylonitrile, and the outer layer is made of aerogel. The invention also provides a preparation method of the fiber. According to the preparation method, a plant essential oil primary aromatic microcapsule is prepared at first. The plant essential oil primary aromatic microcapsule is prepared by the following steps: adding plant essential oil into distilled water containing an emulsifier, and adopting a dual layer capsule wall, the dual layer capsule wall has the advantages that in the subsequent functional modification, the pH change will not damage the essential oil due to the dual layer capsule wall, and at the same time, the fiber has a new function. The inventor accidentally finds that the prepared fiber can store the effective components of natural plant derived fragrance. Moreover, compared with the prior art, the releasing effect of the microcapsule is better, and the releasing time is longer and more uniform.

The invention discloses graphene modified waterborne polyurethane resin and a preparation method thereof. The preparation method specifically includes: subjecting graphene oxide obtained through a Hummers method to functional modification and dispersing to obtain a high-concentration graphene aqueous dispersion; adding polyester polyol (or polyether polyol) and a hydrophilic chain extender which are of formula ratio into the graphene aqueous dispersion, evenly stirring, and removing moisture under a vacuum condition; feeding nitrogen immediately after the vacuum condition is ended, adding diisocyanate of formula ratio, performing heating and stirring reaction for a certain time, then stopping nitrogen feeding, and adding a neutralizing agent; adding deionized water, performing high-speed dispersing for a certain time, then adding a small-molecule chain extender to perform reaction, and discharging. The preparation method has the advantages that the method is simple and controllable inoperation and capable of easily preparing and synthesizing the graphene modified waterborne polyurethane resin in a large-batch manner; the graphene modified waterborne polyurethane resin prepared bythe method is good in adhesive force, corrosion resistance and impact resistance and widely applicable to fields such as the marine field, steel structures, anti-static coating, conductive coating andantifouling and waterproof coating.

The invention provides a carbon material film with high electric conductivity, heat conductivity and strength and a preparing method thereof. The carbon material film is prepared according to the steps of adding functional carbon material powder and a dispersant to a solvent to be mixed and stirred; adding a binder and a plasticizer, and conducting mixing to obtain uniform and stable slurry; forming a blank film; placing the blank film in the solvent for reaction; evaporating the solvent; increasing temperature to 1000-3000 DEG C for sintering lasting 1-10 h to obtain the carbon material film. Through functional modification and then tape-casting and sintering of the surface of the carbon material, active groups between different sheets of the carbon material react with one another to form chemical bonds, and a carbon net stable structure is formed finally so that different sheets of the carbon material can be connected in the true sense. In this way, the mechanical strength, electric conductivity and heat conductivity of the carbon material film are improved greatly.

A polystyrene microsphere is provided. The polystyrene microsphere is micron or nano-scale, and the surface of the microsphere is coated with a polyacrylic resin Eudragit. The polystyrene microsphereof the present invention is a polystyrene microsphere with very low non-specific adsorption, and the surface of the microsphere contains a functional group carboxyl group, so that further functional modification of the surface of the polystyrene microsphere is not required, the operation is further greatly simplified, and the loss is reduced.

The invention discloses a preparation method for polyazobenzene multifunctional nanoparticles based on a rare earth up-conversion material. The preparation method comprises the following steps: 1) synthesizing NaYF4: Tm<3+>, Yb<3+>, Tm<3+> / NaYF4 core / shell up-conversion nanoparticles; 2) coating a silica shell layer onto the surface of hydrophobic UCNPs through a reversed-phase microemulsion method, carrying out functional modification with an MPS silane coupling agent, and allowing particle surface to obtain carbon-carbon double bonds so as to make a preparation for subsequent polymerizationreaction; 3) preparing a double response shell with photoresponse azobenzene as a cross-linking agent and methacrylic acid as a monomer; and 4) etching a silicon layer so as to prepare multifunctionalnanoparticles with rare earth nanoparticles as a core and a double response shell-yolk structure as a shell. According to the invention, by utilization of the up-conversion effect of the rare earth nanoparticles to near-infrared laser, ultraviolet light and visible light are generated, so a microsphere shell azobenzene element can control drug release under the action of a light switch, and a novel idea and a route are provided for solving the problem that stimuli responsiveness of a carrier is limited in an ultraviolet area.

The invention belongs to the technical field of inorganic materials and bioanalysis and in particular relates to an aminophenylboronic acid surface-modified nano-carbon material, as well as a preparation method and application thereof. According to the invention, an available nano-carbon material is subjected to carbodiimide reaction on the basis of acid treatment. Firstly, the surface of the nano-carbon material is modified by polylysine and polyethylene glycol / diglycolic acid, then the nano-carbon material reacts with aminophenylboronic acid for surface functional modification of the nano-carbon material. The synthesis method provided by the invention is simple and efficient. The prepared aminophenylboronic acid-functionalized nano-carbon material, as a nano-adsorbent, has a large specific surface area and strong selective specificity, exhibits better dispersibility and stability in aqueous solutions, and can rapidly achieve the effect of specifically separating and concentrating glycopeptide / glycoprotein. The material is significant in use value and application prospects in the glycoprotein proteomics field and the like.

The invention relates to a hyperbranched polythioether preparation method. According to the preparation method disclosed by the invention, a bi-functionality asymmetric alkene compound and tri-functionality sulfur alcohol are utilized as raw materials, thiol-ene is utilized to perform unequal-reactivity addition reaction, and hyperbranched polythioether is synthesized by a one-pot method. The preparation method disclosed by the invention has the advantages that the raw materials are easy to obtain, and the steps are simple; a terminal group of the prepared hyperbranched polythioether is sulfydryl; functional modification can be further performed; thus, the hyperbranched polythioether with specific performance can be prepared.

The invention relates to cage-shaped oligomerization polyhedral silsesquioxane with eight phenolic hydroxyl groups and a preparation method thereof. Cage-shaped octamer(dimethylsiloxy)silsesquioxane containing a large number of Si-H bonds is prepared by taking low-cost tetraethyl orthosilicate as the raw material through controllable hydrolytic condensation, and then the cage-shaped silsesquioxane with the eight phenolic hydroxyl groups is finally obtained by conducting functional modification on the cage-shaped octamer(dimethylsiloxy)silsesquioxane. The synthesized cage-shaped silsesquioxane with the eight phenolic hydroxyl groups has a Si-O-Si inorganic skeleton structure which is of a nanoscale cage-shaped structure, phenolic hydroxyl functional groups with high reaction activity are arranged on the apex angles, the cage-shaped silsesquioxane can serve as a nanoparticle additive with high reaction activity to be used for resin filler and be bonded in a polymer cross-linked network in a chemical bond mode, the cage-shaped silsesquioxane can serve as a reactive modified additive, and meanwhile the effects of inorganic nanoparticle enhancement and organic chemical crosslinking points are achieved. The preparation technology progress is simple and efficient, and the obtained product is high in yield and high in purity.

The invention discloses a composite particle with multiple core-shell structures. The composite particle uses inorganic silica nanoparticles as a core, uses an organic acrylate polymer as a middle layer, and uses a carbon nanotube adsorption layer as a shell. The invention also discloses a preparation method for the composite particle, first nano-silica is synthesized through a Stober method, thenthe surface of nano-silica is coated with an acrylate polymer layer based on a dispersion polymerization method, and an initiator that can be subjected to hydrolytic ionization is used to achieve coating of the nano silica surface with the polymer and charge modification in one step, then further carbon nanotubes are adsorbed through electrostatic self-assembly. The invention provides the composite particle with multiple core-shell structures and the preparation method thereof, the preparation process is green and efficient, and does not need complicated functional modification, and the prepared composite particle has excellent overall performance.

The invention provides a composite zinc cathode with an inorganic functional modification layer in a water system battery and a preparation method thereof, and application of a composite zinc cathodewith an inorganic functional modification layer in a water system battery. The composite zinc cathode comprises a base layer and a modification layer located on the base layer; the material of the base layer is metal zinc or zinc alloy, and the material of the modification layer comprises ceramic powder and an electronic conductive material.

The invention discloses organic silicone modified styrene-butadiene latex and a preparation method thereof. By adopting a core-shell emulsion polymerization process, the latex takes deionized water as a dispersion medium, and butadiene and styrene as main monomers, adopts an organic silicone monomer for functional modification, and uses reactive emulsifiers, such as allyl ether sulfonates, acrylamide sulfonates, maleic acid derivatives and the like. The particle size of the latex is 120-150nm; the particle size distribution presents relatively narrow monodispersion; the liquidity is good; the viscosity is appropriate; the latex has very good mechanical stability and chemical stability, remarkable improvements on alkali resistance, water resistance, yellowing resistance and the like, and particularly solves the defects that present acrylate emulsion in the market is not good in alkali resistance or water resistance when used on a textile coating of a fiberglass mesh, the coating yellows easily, and the present acrylate emulsion is sticky when heated and fragile when cooled, therefore, the comprehensive performance of the latex is greatly superior to those of the commonly used acrylics and modified emulsion.

A catalase immobilization method on the basis of using an amidoxime PAN nanofibrous membrane as carrier relates to the technical fields of nanofibrous membrane preparation utilizing electrostatic spinning technology, PAN functional modification and enzyme immobilization. The method comprises the following steps: the electrostatic spinning technology is utilized to obtain a PAN nanofibrous membrane, amidoximation modification is performed to the nanofibrous membrane to obtain the amidoxime PAN nanofibrous membrane with stable morphology, high porosity and strong load capacity and biocompatibility, then Cu<2+> is used as centre body, and catalase is immobilized on the amidoxime PAN nanofibrous membrane through coordinate bond. Compared with the conventional catalase immobilization method, the method of the invention solves the defects of catalase immobilization that the recovery rate of enzyme activity is low, the storage instability is low and the reusability is unstable. The immobilized catalase on the basis of using the amidoxime PAN nanofibrous membrane as carrier can be used in various fields such as biosensor preparation, membrane bioreactor and environmentally friendly material.

The invention discloses a portable sensing system for early diagnosing liver cancer and functional modification method of the portable sensing system. The system comprises a micro cantilever array sensing unit, a surface modifying layer, an encapsulation shell, a driving module and a digital display interface processing circuit, wherein the micro cantilever array sensing unit is used for converting a mechanical signal of a cantilever into an electric signal and outputting the electric signal to a digital display interface processing circuit; the surface modifying layer grows on the surface of the micro cantilever array sensing unit and is used for functionalizing the surface of the micro cantilever array sensing unit so that the micro cantilever array sensing unit is capable of combining with a liver cancer landmark antigen specificity; the encapsulation shell is used for protecting the micro cantilever array sensing unit and providing a biochemical reaction sample inlet / outlet and a reaction chamber; the driving module is used for providing a resonant drive signal; and the digital display interface processing circuit is used for obtaining and outputting the liver cancer landmark quality according to the variation of resonant frequency of the micro cantilever. According to the system and method provided by the invention, the early screening of the liver cancer is possible, and the important significance is provided for early diagnosing and early treating the liver cancer and improving the survival rate of the liver rate.