The invention relates to desulfurized gypsum based antistatic self-leveling mortar and its preparation method. Adopting calcined desulfurized gypsum as the main raw material, the desulfurized gypsum based antistatic self-leveling mortar comprises the following components by weight: 25-45 of desulfurized gypsum, 5-10 of slag powder, 3-10 of fly ash, 5-12 of portland cement, 15-25 of quartz sand, 15-25 of steel slag sand, 0.03-0.2 of modified carbon fiber, 0.5-2.0 of redispersible emulsion powder, 0.02-0.07 of cellulose ether, 0.10-0.25 of a water reducer, 0.1-0.4 of an antifoaming agent, 1.2-2.5 of an early strength agent, 0.02-0.06 of a retarder, and 0.002-0.005 of a carbon nanotube. The desulfurized gypsum based antistatic self-leveling mortar has good antistatic performance, bonding property, wear resistance, as well as high compressive strength, and overcomes the defect of poor water proofness of gypsum based materials. By utilizing a lot of industrial solid waste, the self-leveling mortar of the invention has the advantages of environmental protection, energy saving, and low cost, etc., and can be applied in various building ground projects with requirements for electrostatic protection and levelability.