The invention discloses preparation of dendritic molecule-modifiedhydrophilic immunomagnetic beads and application of the hydrophilic immunomagnetic beads to rapid and efficient
cell capture. The preparation method comprises the following steps: firstly, synthesizing
magnetic nanoparticles through a hydrothermal method; hydrolyzing ethyl
orthosilicate and modifying with a silylating
reagent in order that the surface of a material is rich in amino groups;
coupling dendritic molecules to the surface of the material in order that the material is hydrophilic; lastly, fixing an
antibody to the surface of the material to prepare a capture substrate capable of recognizing
tumor cells specifically. As proved by experiments, the hydrophilic immunomagnetic beads modified by the
antibody and the dendritic molecules can shorten the time of combining the material and cells, perform high-specificity recognition and capture
tumor cells rapidly, and capturing efficiency of 86+ / -5 percent can be achieved only in 15 minutes; meanwhile, the activity of the cells is kept, and the
tumor cells can be successfully separated from
whole blood into which a tumor
cell standard is added. A template-free, low-cost and low-
toxicity material is applied to
cell capture, so that the method is novel, convenient, practical and efficient, and has a tremendous clinical application potential.