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683results about How to "Cheap method" patented technology

Methods Using Pores

The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
Owner:OXFORD UNIV INNOVATION LTD

Composite gas separation modules having a layer of particles with a uniform binder metal distribution

A composite gas separation module includes a porous substrate; an intermediate layer at the porous substrate that includes particles and a binder metal, wherein the binder metal is uniformly distributed throughout the intermediate layer; and a dense gas-selective membrane, wherein the dense gas-selective membrane overlies the intermediate layer. In one embodiment, the intermediate layer includes a gradient of particle size from a surface of the intermediate layer proximate to the porous substrate to a surface of the intermediate layer distal to the porous substrate. The particles of the intermediate layer can include particles selected from the group consisting of metal particles, metal oxide particles, ceramic particles, zeolite particles, and combinations thereof.
Owner:WORCESTER POLYTECHNIC INSTITUTE

Preparation method and application of environmental estrogen electrochemical immunosensor

The invention relates to a preparation method and application of an environmental estrogen electrochemical immunosensor, belonging to the technical field of electrochemical detection. The sensitivity of the sensor is obviously improved by adopting the characteristics that the conductivity of chitosan dispersed graphene is high, the stability is high, the specific surface area of a gold hybrid mesoporous silicon dioxide nano composite material is large, the biocompatibility is good, the catalytic efficiency is high and the like. The immunosensor is constructed by adopting the method of layer-by-layer self-assembly of the chitosan dispersed graphene, cross-linking agent, composite solution of nano materials and antibodies, and bovine serum albumin. In combination with specificities of antigens and antibodies, by virtue of an electrochemical workstation instrument, by recording current change before and after corresponding antigens modify the sensor, the environmental estrogens in water and food can be directly detected. The environmental estrogen electrochemical immunosensor prepared by adopting the preparation method has the advantages that the sensitivity is high, the specificity is good, the operation is easy to conduct and the detection limit is low, and the sensitive, rapid and accurate detection of various environmental estrogens in actual samples can be realized.
Owner:UNIV OF JINAN

Nucleic acid screening method of staphylococcus aureus, salmonella, shigella and listeria monocytogenes

The invention discloses a nucleic acid screening method of staphylococcus aureus, salmonella, shigella and listeria monocytogenes, belonging to the field of biological assay. In the method, by virtue of a loop-mediated isothermal amplification (LAMP) technology and a specific primer, the specific area of target genes is amplified by an LAMP technical platform; and with the aid of a positive and negative quality control system and an internal control detection system, nucleic acid screening or detection is carried out on the staphylococcus aureus, the salmonella, the shigella and the listeria monocytogenes from the molecular level. The nucleic acid screening method of the invention has the characteristics of being simple, convenient, economical, fast, sensitive and specific, and the method has wide application prospect.
Owner:中华人民共和国徐州出入境检验检疫局
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