2425 results about "Polyphosphate" patented technology

The present invention describes new compositions of matter in the form of labeled nucleoside polyphosphates with four or more phosphates. In addition compositions of nucleoside polyphosphates with four or more phosphates that are substrates for nucleic acid polymerases with enhanced substrate properties and methods of using these nucleoside polyphosphates for nucleic acid detection, characterization and quantification are described. The compositions provided by this invention include nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogues which have colorimetric, chemiluminescent, or fluorescent moieties, mass tags or an electrochemical tags attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Removal of the polyphosphate product of phosphoryl transfer via phosphate or polyphosphate transferring enzyme leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, a non-hydrolyzable primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and an enzyme having 3′→5′ exonuclease activity which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species characteristic of the sample; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on the detection.

This invention relates to flame retardants for polyester and polyamide compositions, and specifically relates to compositions containing (1) about 30 to about 70 weight percent of a polyester or a synthetic, aliphatic polyamide and synthetic aliphatic, aromatic polyamide copolymers or a mixture thereof; (2) about 15 to about 40 weight percent of glass or mineral reinforcing agent; and (3) a flame retardant of (a) about 5 to about 45 weight percent of melamine polyphosphate; (b) about 15 to about 30 weight percent of melamine polyphosphate and up to about 10 weight percent of a charring catalyst; (c) about 15 to about 30 weight percent of melamine polyphosphate, up to about 10 weight percent of a charring catalyst and up to about 10 weight percent of a char former, wherein all percents by weight are based on the total weight of (1)+(2)+(3) only.

A method of characterizing an analyte sample is provided that includes the steps of: (a) anchoring the analyte to a nucleic acid template of known sequence; (b) conducting a DNA polymerase reaction that includes the reaction of a template, a non-hydrolyzable primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and an enzyme having 3'->5' exonuclease activity which reaction results in the production of labeled polyphosphate; (c) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species characteristic of the sample; (d) detecting the detectable species. The method may include the step of characterizing the nucleic acid sample based on the detection. Also provided are methods of analyzing multiple analytes in a sample, and kits for characterizing analyte samples.

The present invention describes new compositions of matter in the form of labeled nucleoside polyphosphates with four or more phosphates. In addition compositions of nucleoside polyphosphates with four or more phosphates that are substrates for nucleic acid polymerases with enhanced substrate properties and methods of using these nucleoside polyphosphates for nucleic acid detection, charcterization and quantification are described. The compositions provided by this invention include nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogues which have calorimetric, chemiluminescent, or fluorescent moieties, mass tags or an electrochemical tags attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

The present invention relates to a process for producing a water-absorbent polysaccharide including the process steps of bringing into contact an uncrosslinked polysaccharide with a polyphosphate or a polyphosphoric acid as crosslinking agent in the presence of water to form a polysaccharide gel and crosslinking the polysaccharide gel. The invention further relates to a water-absorbent polysaccharide obtainable by this process, a water-absorbent polysaccharide, a composite, a process for producing a composite, a composite produced by this process, the use of the water-absorbent polysaccharides or of the composites as well as the use of polyphosphates.

The present invention relates to improved methods of detecting a target using a labeled substrate or substrate analog. The methods comprise reacting the substrate or substrate analog in an enzyme-catalyzed reaction which produces a labeled moiety with independently detectable signal only when such substrate or substrate analog reacts. The present invention, in particular, describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

A flame retardant thermoplastic composition comprises: a poly(arylene ether); an impact modifier; a polyolefin; a phosphoric acid salt selected from the group consisting of melamine phosphate, melamine pyrophosphate, melamine orthophosphate, diammonium phosphate, monoammonium phosphate, phosphoric acid amide, melamine polyphosphate, ammonium polyphosphate, polyphosphoric acid amide, and combinations of two or more of the foregoing; a metal hydroxide; and an organic phosphate wherein the amount of phosphoric acid salt by weight is greater than or equal to the amount of organic phosphate by weight. The flame retardant composition is may be used in the production of electrical wires.

A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3'->5' exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.

A process for preparing a flowable slurry comprising mixing 25-70 wt. % water; an alkaline material selected from the group consisting of chlorosilicon manufacturing byproducts, direct process residue gels, cement kiln dust, and mixtures thereof; and a process aid selected from the group consisting of sucrose, raffinose, lignin, methylglucopyranoside, lactose, fructose, sodium polyphosphate, trehalose and mixtures thereof to form a flowable slurry. This slurry is especially useful in the manufacture of cement.

The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and / or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates.

A flame-retardant resin composition comprises 10 to 300 parts by weight of a flame retardant (B) and 1 to 200 parts by weight of an inorganic filler (C) (a glass fiber and / or a glass flake), relative to 100 parts by weight of a base resin (A). The flame retardant (B) comprises a polyphenylene oxide-series resin and / or a polyphenylene sulfide-series resin (B1), a phosphoric ester (B2), and a nitrogen-containing cyclic compound (B3) (for example, a polyphosphate of an amino group-containing triazine compound). The inorganic filler (C) has been treated with a surface-treatment agent or sizing agent containing a novolak epoxy resin. Such a resin composition is useful for obtaining a shaped article which has been inhibited from dripping and to which flame retardancy is highly imparted.

The present invention provides novel compounds of dinucleotide polyphosphates and the method of preventing or treating diseases or conditions associated with platelet aggregation. The method comprises administering systemically to a patient a pharmaceutical comprising a purinergic P2τ receptor antagonist, in an amount effective to elevate its extracellular concentration to bind to P2τ receptors and inhibit P2τ receptor-mediated platelet aggregation. Methods of systemic administration include injection by intravenous, intramuscular, intrasternal and intravitreal routes, infusion, transdermal administration, oral administration, rectal administration and intra-operative instillation.

Stain-removing oral compositions, such as gum compositions are herein provided. The compositions include an orally acceptable carrier and a stain-removing anionic surfactant. The surfactant includes a fatty acid salt having at least one hydroxyl functionality. The fatty acid salt may be a salt of ricinoleic acid, and may be combined with a chelating agent and / or an abrasive. The chelating agent may be a polyphosphate and the abrasive may be a silica abrasive.

An aluminum phosphate or polyphosphate-based pigment product is made by a process comprising contacting phosphoric acid with aluminum sulfate and an alkaline solution to produce an aluminum phosphate based product; and optionally calcining the aluminum phosphate based product at an elevated temperature, wherein the process is substantially free of an organic acid. The aluminum phosphate or polyphosphate-based pigment is amorphous. The amorphous aluminum phosphate or polyphosphate characterized by a bulk density of less than 2.30 grams per cubic centimeter and a phosphorus to aluminum mole ratio of greater than 0.8. The composition is useful in paints and as a substitute for titanium dioxide.

A flame retardant thermoplastic composition comprising a poly(arylene ether), a block copolymer, a liquid polyolefin, and a flame retardant additive composition. The flame retardant additive composition comprises a metal hydroxide, an organic phosphate, and a phosphoric acid salt selected from the group consisting of melamine phosphate, melamine pyrophosphate, melamine orthophosphate, melem polyphosphate, melam polyphosphate, diammonium phosphate, monoammonium phosphate, phosphoric acid amide, melamine polyphosphate, ammonium polyphosphate, polyphosphoric acid amide, and combinations of two or more of the foregoing. The block copolymer comprises a block that is a controlled distribution copolymer having terminal regions that are rich in alkylene units and a center region that is rich in aryl alkylene units. The flame retardant composition may be used in the production of covered conductors.

A novel and efficient single pot synthetic schemes are disclosed for preparing modified nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs. The novel method is used to prepare nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs having non-persistent or persistent and non-persistent modifications. Novel biomolecular reactions are also disclosed using the novel modified biomolecules disclosed herein.

A flame-retardant resin composition comprises (A) abase resin; (B) a flame retardant comprising (B1) at least one aromatic resin selected from a pblyphenylene oxide-series resin and a polyphenylene sulfide-series resin, (B2) a phosphoric ester, and (B3) a nitrogen-containing cyclic compound; and (C) a styrenic resin having a melt flow rate of not more than 8 g / 10 minutes. The base resin may be a polyester-series resin. The styrenic resin (C) may be a styrenic resin having a melt flow rate of 0.1 g / 10 minutes to not more than 5 g / 10 minutes. The phosphoric ester (B2) may be a condensed phosphoric ester, and the nitrogen-containing cyclic compound (B3) may be a polyphosphate of an amino group-containing triazine compound, a polyphosphoric acid amide, or others. The present invention provides a flame-retardant resin composition which has been flame-retarded without using a halogen-containing flame retardant.

A composition comprises a compatibilized poly(arylene ether) / polyamide blend, an optional electrically conductive filler, a phosphinate, and flame retardant augment selected from the group consisting of melamine polyphosphate, zinc borate, low melting glass, talc, and combinations of two or more of the foregoing flame retardant augments.

The fire retardant composition consists of pentaerythritol phosphate 18-54 wt%, melamine polyphosphate 40-80 wt%, stearic acid or zinc stearate 0.1-5 wt%, coupling agent 0.1-5 wt% and stuffing 0.1-5 wt%. It is used as fire retardant for polypropylene powder or grains, and when the addition amount is 28 wt% fireproof level of UL94V-0 is reached; and is also used as fire retardant for polyethylene,and when the addition amount is 30 wt% fireproof level of UL94V-0 is reached.

The present invention relates to an aqueous oral composition comprising:a) from 0.2% to 3% divalent metal ions comprising:i. from 0.1% to 1.5% of zinc ions;ii. from 0.1% to 2% of tin (II) ions; andb) a source of fluoride ions;c) a silica dental abrasive;d) one or more chelants having a MW of less than 1000 and capable of forming water-soluble complexes with the zinc ions, wherein the chelants comprise less than 0.2% linear polyphosphates having a chain length of four or more;e) an orally acceptable carrier comprising at least 20% total water;wherein the pH of the composition is from 5 to 6.5, the molar ratio of the chelants to the divalent metal ions is at least 0.70:1 and at least 80% by weight of the total zinc ions are solubilised within the composition.The composition of the invention has been found to give improved antimicrobial activity from the zinc / stannous combination without significant taste, staining or stability problems, compared to compositions having lower levels of chelants.

The invention provides a method for processing the surface of magnesium-lithium alloy. The technique of micro-arc oxidation is successfully applied to processing the surface of the magnesium-lithium alloy. Direct current / DC pulse / alternating current pulse multiplex power supply is used and the magnesium-lithium alloy with different lithium contents in a compound electrolyte system of sodium polyphosphate, sodium silicate, calgon and sodium hydroxide is carried out by micro-arc oxidation for 2-120min. A ceramic oxide film of 10-100Mum with a rigidity higher than 200Hv is formed in situ on the surface of the magnesium-lithium alloy, thus remarkably improving corrosion resistant property compared with an alloy matrix, solving the problem known at home and abroad concerning processing the surface of the magnesium-lithium alloy and broadening the application in the fields of aviation, aerospace, car, electronic, etc.

A process for the preparation of amorphous aluminum phosphate or polyphosphate-based pigment by reacting aluminum phosphate and sodium aluminate is provided. The amorphous aluminum phosphate or polyphosphate is characterized by a skeletal density of less than 2.50 grams per cubic centimeter and a phosphorus to aluminum mole ratio of greater than 0.8. In one embodiment, the composition is useful in paints as a substitute for titanium dioxide.

The present invention relates generally to the use of terminal-phosphate-labeled nucleotides having three or more phosphates as substrates for nucleic acid polymerases and their use in DNA amplification. The labels employed are chemiluminescent, fluorescent, electrochemical and chromogenic moieties as well as mass tags and include those that are directly detectable, detectable after enzyme activation or feed into other processes to generate a different signal. The signal generated from the attached dyes may also be used to quantify the amount of amplification. Further provided are stabilizers that enhance the stability of terminal-phosphate labeled nucleoside polyphosphates in aqueous solutions and are useful for reducing non-enzymatic hydrolysis of these nucleotides, hence decrease background.

A composition, preferably a halogen-free, flame retardant composition, comprising in weight percent based on the weight of the composition:A. 1 to 90% TPU polymer,B. 1 to 90% polyolefin polymer, preferably a polar polyolefin polymer,C. 1 to 60% phosphorus-based, intumescent flame retardant,D. 0.5 to 25% liquid phosphate modifier, e.g., bis-phenol-A-polyphosphate, andE. Optional additives and / or fillers.The compositions exhibit excellent strain and scratch whitening performance in combination with excellent burn performance, good flexibility and tensile properties, and good fabrication extrusion characteristics including improved surface smoothness.

A flame retardant additive composition comprising a phosphoric acid salt selected from the group consisting of melamine phosphate, melamine pyrophosphate, melamine orthophosphate, ammonium phosphate, ammonium phosphate amide, phosphoric acid amide, melamine polyphosphate, ammonium polyphosphate, ammonium polyphosphate amide, polyphosphoric acid amide and combinations of two or more of the foregoing; a metal hydroxide; and an organic phosphate. The flame retardant additive is useful in a wide range of thermoplastic compositions.