113 results about "Estriol" patented technology

The present invention discloses administering steroid hormones to mammals to treat autoimmune related diseases, more particularly, Th1-mediated (cell-mediated) autoimmune diseases including: multiple sclerosis (MS), rheumatoid arthritis (RA), autoimmune thyroiditis and uveitis. Most preferably the invention is used to treat a patient with a therapeutically effective amount of estriol of 8 milligrams once daily via oral administration to treat the symptoms or prevent the onset of multiple sclerosis.

The present invention relates to pregnancy hormone combinations and methods of treatment for autoimmune diseases having at least two hormonal components, a pregnancy hormone (such as estriol), and a gestagen (such as levonorgestrel or norethindrone) thereby providing for the continuous, uninterrupted administration of pregnancy hormones for the treatment for autoimmune disorders, such as multiple sclerosis.

The present invention discloses administering steroid hormones to mammals to treat autoimmune related diseases, neurodegenerative diseases or disorders, such as Alzheimer's disease, Parkinson's Disease, multiple sclerosis, stroke, ALS Pick's disease, prion disease and Huntington's disease. Most preferably the invention uses estrogens, estranges, estriol or estrogen receptor active agents to prevent or ameliorate clinical symptoms of the diseases and disorders.

The present invention discloses administering steroid hormones to mammals to treat autoimmune related diseases, neurodegenerative diseases or disorders, such as Alzheimer's disease, Parkinson's Disease, multiple sclerosis, stroke, ALS Pick's disease, prion disease and Huntington's disease. Most preferably the invention uses estrogens, estranges, estriol or estrogen receptor active agents to prevent or ameliorate clinical symptoms of the diseases and disorders.

The invention relates to the use of low doses of estriol by vaginal route for treatment and / or prevention of urogenital atrophy due to estrogen deficit in women.

A topical composition for relief from certain menopausal, peri- and post-menopausal symptoms is disclosed, consisting of a low dosage of progesterone, testosterone and estriol in a pharmaceutically acceptable topical carrier in a weight ratio of from about 1:0.1:0.01 to about 1:0.1:0.02, respectively. In a preferred single dose, the three active ingredients are present as follows: (a) from about 20 to about 35 mg progesterone; (b) from about 2 to about 3.5 mg testosterone; and (c) from about 0.2 to about 0.7 mg estriol. A preferred carrier is cocoa butter, and a preferred single dose form is a vaginal suppository.

A pharmaceutical composition for treatment of skin aging including a pharmaceutically effective amount of estriol; a pharmaceutically effective amount of estradiol; a pharmaceutically effective amount of hyaluronic acid; a pharmaceutically effective amount of green tea extract; and a pharmaceutically acceptable carrier is provided. In some embodiments, the composition further includes ascorbic acid, date palm extract, or a combination thereof. Also disclosed is a method for treating symptoms of skin aging and photoaging by administering a pharmaceutical composition having estriol, estradiol, hyaluronic acid, green tea extract, a pharmaceutically acceptable carrier and optionally, ascorbic acid and date palm extract. A method of promoting urogenital function is likewise provided wherein a pharmaceutical composition having estriol, estradiol, hyaluronic acid, and green tea extract is applied intravaginally.

The invention relates to a detection technology of endocrine disrupters in water environment, in particular relating to a method for detecting seven substances such as oestrone, 17 beta-estradiol, estriol, 17 alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A and the like in a water environment sample by virtue of liquid chromatogram-tandem mass spectrometry. The method comprises the following steps: concentrating and gathering the estrogen, the nonyl phenol, the octylphenol and the bisphenol A in a water sample by utilizing a solid phase extraction column; eluting the estrogen, the nonyl phenol, the octylphenol and the bisphenol A from the solid phase extraction column by utilizing ethyl acetate; and analyzing the concentrations of the estrogen, the nonyl phenol, the octylphenol and the bisphenol A by virtue of liquid chromatogram-tandem mass spectrometry. The method has the advantages of environment friendliness and easiness for operation, ensures relatively high recovery, and can be used for fast analyzing the concentrations of the estrogen, nonyl phenol, octylphenol and bisphenol A in the water sample.

The invention relates to a detecting technique of endocrine disrupters in water environment and particularly relates to a technique for quantitative analysis on oestrone, 17beta-estradiol, estriol, 17alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A in a complex substrate water sample by adopting a liquid chromatogram-tandem mass spectrum combined technique. The method comprises the following steps: enriching a collected water sample by using a HLB (Hydrophile-Lipophile Balance) solid-phase extraction column and washing with carbinol; drying elution liquid nitrogen and purifying by using Florisil; and finally, analyzing by utilizing the liquid chromatogram-tandem mass spectrum combined technique. The method is environment-friendly, easy to operate and high in recovery rate. The method can quickly analyze the trace amount of oestrone, 17beta-estradiol, estriol, 17alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A in the complex substrate water sample.

The invention discloses a derivatization method of environmental estrogens (estrone, 17beta-estradiol, estriol, 17alpha-ethynylestradiol), which is a treatment method and a core technology before a gas chromatography-mass spectrum (GC-MS) is used for analyzing the samples of the substances. The method comprises the following steps: firstly, leading high-purity nitrogen in at a room temperature to dry a standard mixed solution containing environmental estrogens; adding derivative reagents N, O-BSTFA and pyridine to react at room temperature (20 DEG C)-50 DEG C to obtain derivative products; drying; adding a sampling solvent and an internal standard; sampling and analyzing. The method has the advantages of simple and convenient experimental operation, time saving and low energy consumption and cost, simultaneously derivates the four environmental estrogens without generating side products, has favorable linear correlation among various derivative products and achieves an instrument detection limit up to 0.5pg / muL, thereby being applied to the GC-MS detection of environmental estrogens in water bodies, sediment, biological samples, and the like.

Pseudomonas fluorescens capable of degrading estrogen substances is named as SJTE-2, and a preservation number is CGMCC No.6587. The pseudomonas fluorescens can use natural estrogen or environmental estrogen as the only carbon source to grow and can degrade estrogen substance oestrone, 17-Beta-estradiol, estriol and polycyclic aromatic hydrocarbon substance naphthalene, luxuriant, bisphenol A, and can be used for decomposing and removing an estrogen substance and a polycyclic aromatic hydrocarbon substance in an environment. Under the culture condition that the estrogen or the polycyclic aromatic hydrocarbon is used as the only carbon source, a bacterial strain SJTE-2 can degrade oestrone, 17-beta-estradiol and estriol within 24 hours, wherein the initial concentration of the oestrone, 17-beta-estradiol and estriol is 1 mg / L, and degradation rate of naphthalene, luxuriant and bisphenol A is over 90%, wherein the concentration of the naphthalene, luxuriant and bisphenol A is 50 mg / L. The bacterial strain can degrade oestrone, 17-beta-estradiol and estriol for more than 90% in 7 days, wherein the initial concentration of oestrone, 17-beta-estradiol and estriol is 50 mg / L, and degradation rate of naphthalene, luxuriant and bisphenol A is larger than 80%, wherein the concentration of naphthalene, luxuriant and bisphenol A is 500 mg / L.

The present invention discloses administering steroid hormones to mammals to treat autoimmune related diseases, including post-partum auto immune diseases) and more particularly. Most preferably the invention uses estrogens, estranges, estriol or estrogen receptor active agents to prevent or ameliorate clinical symptoms of these Th1-mediated (cell-mediated) autoimmune diseases known to either have an initial onset following the birth of a child or which are exacerbated in patients in the post-partum period.

Methods, compositions and kits for treating psoriasis and other autoimmune diseases in human or animal subjects. The disclosed methods generally comprise administering to the subject a therapeutically effective amount of a naturally occurring or synthetic agent comprising estriol or another estrane, estrogen or estrogen receptor-effective composition. The disclosed compositions and kits generally comprise topical preparations and / or combination preparations wherein 2 or more agents are combined for either simultaneous administration or administration at different times and by the same or different route(s) of administration.

The invention discloses an enzyme immunoassay method for progestational hormone in excrement of sows. The method comprises the steps of: (1) sample collection: collecting the excrement of the sows, weighing and dissolving 0.5 g of excrement of the sows into 20 ml of measuring buffer liquid, carrying out centrifuging, taking and placing supernatant at 4 DEG C to be detected; and (2) measuring content of progestational hormone in a sample by utilizing competitive ELISA (enzyme-linked immuno sorbent assay): measuring according to an ELISA process. According to the enzyme immunoassay method for the progestational hormone in the excrement of the sows, the detection sensitivity of progesterone reaches 0.2 ng / ml or 8 ng / g; the recovery rate of the progesterone is 95.78%; inter-assay and intra-assay variable coefficients are respectively 6.8% (n is equal to 9) and 11.23% (n is equal to 8); the specificity of the progesterone is that the cross reaction rates of the progesterone to estradiol, estriol and oestrone are all less than 0.01%, and the cross reaction rate of the progesterone to testosterone is 0.35%; the stability is good; the required sample is easy to collect, animals are not injured, a measured result is reliable, and a method for measuring blood of the animals can be replaced to monitor a series of reproductive activities of the animals.

The invention provides a non-invasive assay for the detection of a SLOS affected individual by determining the ratio of at least one of the specific SLOS steroid analytes, dehydro-estriol (8-DHE3) and a dehydro-pregnanetriol (7-DHPT) to their normal steroid counterparts estriol (E3) and pregnanetriol (PT), respectively. 8-DHE3 and 7-DHPT represent metabolites which accumulate in the blood and urine of an individual with SLOS or an individual carrying a SLOS affected fetus. The invention provides for a reliable and reproducible method of screening women for SLOS affected fetuses early on in pregnancy.

The invention relates to a pseudomonas citronelloalis strain and application thereof in degrading estrogen, wherein the pseudomonas citronelloalis strain has the preservation number of CGMCC No.3634, can grow by using natural or synthetic estrogen as a unique carbon source, can efficiently degrade theelin (E1), 17beta-estradiol (E2) and 17alpha-ethinyl estradiol (EE2) and can be used for a micro-biological degradation technology for removing the estrogen. Under the culture condition of using the estrogen as the unique carbon source, the theelin and the 17beta-estradiol with the initial concentration of 2mg / L can be respectively degraded by 99 percent by the SS-2 strain within 36 hours, the 17alpha-ethinyl estradiol with the initial concentration of 4mg / L can be degraded by 93.6 percent within 168 hours, and estriol has no remarkable degradation phenomenon. The theelin is generated in the process of degrading the 17beta-estradiol, and the theelin is also gradually degraded. Because the pseudomonas citronellolis SS-2 strain has the characteristics of effectively degrading the natural and synthetic estrogen in an environment, the pseudomonas citronellolis SS-2 strain can be applied to an estrogen-contained waste sewage treatment technology or a soil remediation technology.

The invention relates to the technical field of biosensors and discloses an estriol hapten and antibody and the application thereof in a fast sensor method for uncojugated estriol detection. The structure of the hapten is scientifically designed, so that sensitivity and specificity of the antigen-antibody are improved; electro-deposition of a prussian blue-chitosan-nanogold composite membrane on the surface of an electrode is conducted, the estriol antigen is fixed to the surface of the membrane, a novel horse radish peroxidase labeled estriol antibody and a sample to be tested are added after an unbound site is sealed, the sample and a fixed antigen compete for a free enzyme labeled antibody, hydrogen peroxide is added after washing, a current signal is measured, a cyclic voltammetry estriol standard curve is obtained, the fast sensor method for uncojugated estriol detection is provided successfully, detection range is 0.001-100 ng / mL, and detection limit is 0.001 ng / mL. The method has the advantages of being easy to operate, high in sensitivity, quick, convenient and the like and can be well applied to ultra-sensitive and fast detection of estriol in the sample.

The present invention relates to the fields of analytical chemistry and food safety, and provides a method for simultaneously detecting residue quantities of multiple hormone veterinary drugs. The method comprises steps of: extracting a sample with acetonitrile; purifying by three SPE columns, including a C18 column, a silica gel column and an amino column; carrying out a microwave assisted derivatization reaction; and using a DB-5MS capillary column (0.25mm *30m *0.25 mum) to separate and detect residue quantities of nine hormone veterinary drugs. Detection limits of the method of the invention on the nine hormone veterinary drugs, including hexestrol, diethylstilbestrol, dienestrol, etiocholanolone, epitestosterone, oestrone, estradiol, ethinyloestradiol and estriol, are 0.1 mug / kg, 0.3 mug / kg, 0.15 mug / kg, 1mug / kg, 0.17 mug / kg, 0.3 mug / kg; 0.4 mug / kg, 0.35 mug / kg and 0.3 mug / kg respectively. The lower limit of detection of the method provided by the invention meets requirements of related domestic and foreign laws and regulations, and technical indicators, such as precision and recovery rate, are in line with the provisions of relevant standards.

The invention relates to an oat acidovorax avenae strain capable of biodegrading natural estrogen and the application thereof, and the preservation code is CGMCC No. 3633; natural estrogen serves as the only one carbon source and is grown, can degrade estrone, 17Beta-estradiol and estriol with high efficiency, and can be used for a microbial technology for removing estrogen. The SS-1 strain can degrade the estrone with the initial concentration of 1mg / L by 99 percent within 12h, and degrade the 17Beta-estradiol and estriol with the initial concentration of 1mg / L by 99 percent within 24h. Estrone is generated in the degradation process of 17Beta-estradiol, and the estrone is also gradually degraded. Because the acidovorax avenae SS-1 strain has the property of effectively degrading the natural estrogen in the environment, and thereby can be applied in a technology for controlling wastewater and sewage which contain estrogen or a soil restoration technology.

The invention discloses a test strip for detecting estriol and applications thereof. The test strip comprises a sample absorbing pad (1), a combined substance releasing pad (2), a reaction membrane (3), a water absorbing pad (4), and a bottom plate (7). The reaction membrane comprises a detection line (5) embedded with an estriol semi-antigen-carrier protein conjugate and a quality control line (6) embedded with a goat-anti-mouse anti-antibody. An estriol monoclonal antibody-colloid gold marker is sprayed on the combined substance releasing pad (2). The invention also provides a method using the provided estriol test strip to detect residual estriol in fish meat, chicken, shrimp meat, and pork. The provided test strip has the characteristics of simple operation, high sensitivity, fast detection speed, and low cost, and is suitable for screening of massive samples and onsite monitoring.

Film-shaped medicaments administered orally, particularly buccally, for treating climacteric complaints. The medicaments contain estriol and / or at least one pharmacologically acceptable ester of estriol, either alone or in combination with at least one gestagen.

A pharmaceutical dosage unit comprising drospirenone and an estrogen sulphamate, such as an estradiol sulphamate or an estriol sulphamate for use in hormone replacement therapy is disclosed. This, combination therapy may comprise continuous or discontinuous administration of drospirenone and / or the estrogen sulphamate, such as weekly administration of both agents or weekly administration of the estrogen sulphamate and daily administration of the drospirenone.

The invention relates to a method for rapidly detecting steroid hormones in urea through an UPLC-MS / MS technology, and belongs to the field of analysis chemistry and medical science. The concentrations of estriol, hydrocortisone, 17beta-estradiol, testosterone and progesterone in human urea are simultaneously detected through the UPLC-MS / MS technology by adopting a beta-glucuronidase enzymatic hydrolysis technology, the detection limit is 0.1-1.0 ng / mL, and the quantitation limit is 0.3-3.0 ng / mL. The method is fast, sensitive and accurate, can meet requirement of simultaneous detection of above five steroid hormones in urea, and has wide application prospect.

The invention discloses an estriol homogeneous phase enzyme immunoassay reagent as well as a preparation method and a detection method thereof and belongs to the technical field of immunoassay reagents. An estriol immunogen prepared by the invention is high in immunogenicity, can be induced to obtain a high-titer anti-estriol specific antibody and does not have any cross reaction with 62 common drugs. The estriol immunoassay reagent prepared by the antibody is capable of accurately and rapidly determining the estriol content in biological samples such as urea, blood and the like. Compared with the existing assay reagents in the market as well as detection methods and preparation methods thereof, the detection reagent disclosed by the invention has the advantages of simplicity and convenience in operation, high sensitivity, high specificity, accurate result and the like, is capable of effectively reducing the estriol detection cost and is favorable for large-scale clinical popularization and application.

The invention discloses a system for removing antibiotics and estrogen in livestock excrement through Fenton oxidation. The system comprises a tail gas treatment system, a Fenton reaction system and a dewatering and water discharging system, wherein the Fenton reaction system comprises a reaction tank, a stirrer motor, a telescopic motor, a telescopic stirring shaft, an impeller, a feeding opening, an exhaust opening, a reagent spray head, a material scraping device, a ventilation pipe, a material discharging opening, a heating device, a water inlet, an annular spraying pipe, a temperature sensor, a temperature controller and a support frame; the exhaust opening is connected with the tail gas treatment system; the material discharging opening is connected with the dewatering and water discharging system; excrement slurry is transferred into the dewatering and water discharging system to be subjected to solid-liquid separation. The system disclosed by the invention has the advantages that the equipment flow process is simple and effective; the occupied ground is small; the treatment is fast; the investment cost is low; cleanness and reliability are realized; the tail gas is effectively treated; meanwhile, the antibiotics and estrogen in livestock excrement are removed; the one-time removal effect on sulfadimethoxypyrimidine, estriol, estradiol, bisphenol A and ethinyloestradiol in the livestock excrement can reach 83.37 percent to 96.22 percent.

The present invention relates to methods and kits useful for predicting the time of onset of labour in a pregnant subject. In particular, the invention relates to methods and kits for predicting the time of onset of labour wherein the levels of at least two hormones, selected from estriol, estradiol and progesterone, are determined and a ratio of said levels is calculated, and wherein the time of onset of labour is predicted by comparison of said ratio with a predetermined ratio. The invention further contemplates methods for preventing preterm delivery of an infant / offspring.

The invention discloses a steroid environment endocrine disturbing chemicals hydroxyl group / keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals (oestrone, 17 beta-estradiol, 17 alpha-ethinyl estradiol, estriol and progesterone). The method comprises the following steps: first fetching standard mixed solution containing the steroid environment endocrine disturbing chemicals and internal standard and introducing high-purity nitrogen at normal temperature so as to dry the standard mixed solution; and adding derivatization reagent prepared from N-methyl-N-trimethylsilyl trifluoroacetamide, iodotrimethylsilane and dithiothreitol based on a proportion of 1,000 muL:5 to 10 muL:5 mg and reacting at 20 to 60 DEG C for 5 to 10 min so as to obtain the derivatization product shown in figure. The method has the advantages of simple and time-saving operation, low energy consumption and cost and the like. The hydroxyl group and the keto group can be derivatized synchronously, the linear correlation among the derivatization products is good, and the detection limit of instrument can reach 0.01 to 1 pg / mu L. The method can be used for the GC-MS detection of the steroid environment endocrine disturbing chemicals in samples such as water, bottom sediment, organisms and the like.

The invention relates to the technical field of breeding of small yellow croakers, in particular to a feminization inducing method for small yellow croakers with undifferentiated sex glands. The method comprises the following steps: placing fry of small yellow croakers hatched for 13-15 days and having undifferentiated sex glands in seawater containing estrogen with a low concentration of 1-10 [mu]g / L for dipping bath, and performing dipping bath for 2 hours every day and continuous for 60 days, so as to enable the crowd of small yellow croakers to be completely feminized, wherein the selectable estrogen comprises estrone, soybean isoflavone, estradiol, estriol, coumestrol and genistein. The effect of estradiol is more obvious, 1 [mu]g / L estradiol can ferminize 96.6% of a crowd of small yellow croakers, 10 [mu]g / L estradiol can ferminize 100% of a crowd of small yellow croakers, the induction rate is high, the operatability is high, natural environment does not cause limit. Female parent fish guarantee is provided for large-scale artificial breeding of small yellow croakers, a technological route is opened up for study on sex control and monosex breeding of small yellow croakers, and a route for solving the problem of less of female population of small yellow croakers is provided.