796 results about "Marine fish" patented technology

The present invention discloses a natural fruit and vegetable ferment beverage, and a preparation method thereof. The preparation method for the ferment beverage comprises: adding freshly-squeezed fruit and vegetable juice to marine fish skin collagen peptide; adopting the resulting mixture as a fermentation substrate; carrying out dilution, slurry mixing and sterilization, and then adopting a direct vat and freeze-dried type lactic acid starter to carry out fermentation; stopping the fermentation after 7-11 days until the pH value is 4.3; adding a supplementary feed and carrying out sterilization; then adding high-activity dried yeast to carry out fermentation; stopping the fermentation after 5-7 days until the pH value is 4.0; after storing the fermentation broth at the low temperature, carrying out homogenization, concentration, centrifugation, blending, sterilization and bulking to finally prepare the novel natural fruit and vegetable ferment beverage. According to the ferment beverage of the present invention, the stepwise fermentation is adopted for the freshly-squeezed fruit and vegetable juice by using the lactic acid bacteria and the yeast; the marine fish skin collagen peptide is added to promote the growth and the metabolism of fermentation bacteria, such that metabolic products of nutritional ingredients in the natural fruits and vegetables are rich so as to provide a beneficial nutrition and health effect.

The invention relates to a water circulation system and a method for breeding marine fishes inland, and mainly solves the technical problems of complex process, large investment, high operating cost, large limitation of breeding varieties, low survival rate, slow growth and the like in the process of breeding the marine fishes in fresh water or low salinity salt water at present. A technical scheme comprises the following steps of: a, establishing the water circulation system; b, performing domestication on juvenile fishes of the marine fishes in the low salinity salt water in a gradient mode, namely breeding the juvenile fishes which are bred in the seawater in the brackish water temporarily, formulating a domestication strategy of slowing salinity gradually, and performing domesticationon the juvenile fishes of the marine fishes through three gradients, namely the brackish water phase, the low salinity phase and the ultra-low salinity phase; c, adding a physiological salt preparation, namely adding metal ions which play a key role in osmoregulation into the water or a feed; and d, managing the breeding, namely providing a preference temperature, illumination intensity and a daily illumination period. The water circulation system and the method are mainly used for breeding the marine fishes inland.

The invention relates to a method for making a pet food attractant, which takes duck liver and marine fish as a main material, wherein the addition of the duck liver is 50-100%. The method comprises the following steps: mincing the raw material by a mincer, adding a proper amount of water for disinfection, adding protease for enzymatic hydrolysis, adding amino acid and carbohydrate for carrying out maillard reaction after the enzymatic hydrolysis reaction is finished, spray drying to obtain the food attractant specially used for pet, wherein the water content of the finished product is 6-10%.According to the enzymatic hydrolysis method in the invention, the partial protein is degraded to a polypeptide substance which is easy to be absorbd by pet, The specific local flavor food attractant is prepared by controlling the maillard reaction condition and the food attractant product is finally prepared, the food attractant with dark red color has rich meat fragrance, thereby the initiative for ingestion of pet can be enhanced for first time. The food attractant is capable of applying to exploitation of various pet principal food and snacks products.

The invention relates to a marine-fish environmental protective compound feed, consisting of the following materials in weight ratio: 13 to 20 portions of domestic fish powder, 8 to 15 portions of biological fish meal ABP600, 8 to 12 portions of soybean meal, 10 to 18 portions of peanut bran, 3 to 10 portions of corn, 3 to 7 portions of cotton seed meal, 3 to 7 portions of rapeseeds meal, 3 to 5 portions of meat and bone meal, 3 to 5 portionsof squid bowels powder, 20 to 30 portions of high gluten flour, 1.0 to 3.0 portions of blood meal, 1.0 to 2.0 portions of soybean lecithin, 2.0 to 3.0 portions of marine-fish oil, 0.3 to 0.6 portions of choline chloride (50%), 0.05 to 0.1 portions of Vitamin C phosphate (35%), 0.15 to 1.5 portions of calcium biphosphate, 0.2 to 0.5 portions of compound multiplex vitamin, 0.5 to 1.5 portions of compound mineral salt, 0.1 to 0.3 portions of feed attractant, 0.1 to 0.3 portions of micro-bio-preparation, 0.03 to 0.08 portions of A3Alpha peptidoglycan, 0.01 to 0.04 portions of phytase, and 0.04 to 0.08 portions of non-starch polysaccharase. The compound foodstuff can meet the nutrition demands of such high density cultivated marine fishes such as weever, large yellow croaker, American red fish, sparidae fishes, and trachinotus ovatus and rudd etc, and the cultivated objects can rapidly grow and has high foodstuff conversion rate; the compound foodstuff can intensify the immunity and anti-stress ability of fishes and endow them with good vitality, vivid body color and high survival rate; compared with marine fished cultivated by other foodstuffs, the excretion rates of nitrogen and phosphorous of the marine fishes cultivated by the compound foodstuffs is lowered by 10 to 15 percent, and cause smaller contamination to the cultivation water body.

The invention discloses a garden stuff, spice, edible flower, marine fish and shrimp combined sweet, sour, hot and delicious sauce. The sauce is prepared from main materials and auxiliary materials through the steps of combining the main materials and the auxiliary materials, and then, adding seasonings including white sugar, stevia rebaudiana, table salt, shallot, ginger, garlic and pepper, wherein the main materials include one or more selected from fresh fruits, fresh vegetables, botanical spices and edible flowers, and the auxiliary materials include marine fishes and shrimps. According to the invention, the main materials including fruits, vegetables, botanical spices and edible flowers are rich in various celluloses, vitamins, enzymes, polysaccharides, polyphenol and other natural nutritional ingredients, botanical spices provide special fragrance for the sauce, the delicate flavor of the sauce is increased through adding the marine fish and shrimp components, and the protein component is additionally provided, so that the nutritional value of the sauce is increased. The garden stuff, spice, edible flower, marine fish and shrimp combined sweet, sour, hot and delicious sauce is prepared from pure natural raw materials, is high in nutritional value, sweet, sour, hot and delicious in flavor through natural fermentation, free of additives and special in taste, and can be eaten instantly, dipped, used for cold dressing and cooking and eaten with rice or bread.

The invention relates to a composition for enhancing immunity and a preparation method of the composition for enhancing the immunity. The composition comprises the following raw materials: cordyceps sinensis powder, marine fish oligopeptide, corn oligopeptides, wheat oligopeptides, American ginseng extract, ganoderma extract, medlar extract and ganoderma spore powder. According to the preparation method, the cordyceps sinensis powder and three short peptide materials with biological activity are combined, and are supplemented with the plant extract capable of improving the immunity, therefore, the effect of enhancing the immunity can be achieved.

The invention provides a grouper environmentally-friendly soft granulated feed and a preparation method thereof. The grouper environmentally-friendly soft granulated feed comprises the following nutritional components in percentage by weight: 40 to 50 percent of crude protein, 6 to 12 percent of crude fat, 8 to 15 percent of crude ash, 22 to 28 percent of carbohydrate, and 6 to 10 percent of moisture. According to the invention, the high-quality grouper environmentally-friendly soft granulated feed is studied, popularized and applied, so that the nutrition of the launched feed goes to be complete and balanced, and the palatability of the feed is good, not only can the feed digestive absorption rate of grouper be improved, but also the survival rate and growing speed of the grouper are improved, and the fish quality is improved. Therefore, non-polluted fish products are produced, and the cultivation economical benefit is improved; and the feed has active and profound significance in reducing the usage of small trash fish, lightening the sea area pollution, protecting the marine eco-environment, and promoting the sustained stable and health development of the marine fish culture industry.

The invention provides a compound feed capable of improving the fatty liver and body colour of the marine fish. The compound feed comprises a premixed feed, a bile acid, curcumin and betaine, wherein the premixed feed comprises fish meal, soya bean meal, corn gluten meal, high protein flour, corn, shrimp shell meal, monocalcium phosphate, fish oil, liquid phosphatide, vitamin premixes, mineral premixes, and the like. Artificial marine-fish breeding experiments prove that the compound feed provided by the invention can meet the nutritional needs of marine-fish growth, effectively enhance the physique of the marine fish, improve the immunity of the marine fish, reduce the incidence of a fatty liver disease, and improve the body color of the marine fish, so that the body color of the marine fish is bright-colored and close to a wild natural color, thereby enhancing the quality of artificially cultured marine fish.

The invention relates to a method and a device for fish flesh detection. The method comprises the following steps of 1, acquiring a hyperspectral image of a fish flesh sample, wherein the hyperspectral image comprises spectral data of multiple wavebands, 2, extracting the spectral data of N feature wavebands, wherein N is greater than 1, and 3, utilizing the extracted spectral data of the N feature wavebands as an input value of a learning vector quantization model, and identifying if the fish flesh sample belongs to marine fishes or freshwater fishes according to an output value of the learning vector quantization model. The method and the device can realize fast and accurate identification of marine fishes or freshwater fishes and can realize fast and accurate detection of mercury content of fish flesh.

The invention provides a nutritional composition suitable for patients suffering from bone and joint diseases. The nutritional composition is prepared from 20%-40% of marine fish oligopeptides, 10%-30% of ossein protein peptides, 1%-5% of organic calcium, 1%-5% of casein phosphopeptides, 0.1%-1% of a saussurea involucrate culture, 1%-5% of shark cartilage powder, 5%-20% of inulin, 5%-30% of oligopeptides, 0.1%-1% of vitamins, 1%-3% of mineral substances and the like. The marine food which is used for special dietary uses, is suitable for being eaten by the patients suffering from the bone and joint diseases and contains the marine fish oligopeptides and the ossein protein peptides is provided according to physiological characteristics, nutritional requirements and conventional diet defects of the patients suffering from the bone and joint diseases, and the food has the effects of improving the nutritional condition of the organism, promoting rehabilitation of bones and joints, assisting in controlling blood glucose and conditioning the intestinal functions of the organism and the like.

The invention relates to a hatching device and method for demersal spawns of marine fishes. The hatching device comprises a net cage, a water channel, a bracket, a temperature controller indicator, a probe, a heating rod, a water inlet hose, a sinker, an air inlet hose, an air stone and an annular water injector, wherein the net cage and the bracket are located in the water channel; the net cage is bound on the bracket; the water inlet hose and the air inlet hose are located in the water channel outside the net cage; the upside of the net cage is provided with the annular water injector; and the probe and the heating rod are arranged in the water channel. The hatching method comprises the following steps of: controlling a hatching condition; hatching; carrying out microscopic examination observation; carrying out secondary water temperature controlling; managing routinely; collecting larva fish and the like. The hatching method has the advantages that spawn deposition is avoided because of conical point inflation; spawns adhered on net cage edges are washed at any time by the annular water injector; the net cage is conveniently replaced; the water temperature is stably controlled by an electric temperature controller; and the fresh water quality can be ensured due to water feeding and drainage modes of water feeding of the annular water injector at the upside and water feeding of the water channel at the bottom and overflowing of the periphery of the water channel. Therefore, by means of the hatching device and method, a hatching rate of the demersal spawns of the marine fishes in the industrial fry breeding is improved.

The invention relates to a feed additive premix for low-salinity cultured marine fishes. The feed additive premix for the low-salinity cultured marine fishes consists of multi-vitamins and composite trace elements, and 20 to 30kg of feed additive premix is added into each ton of marine fish feed, wherein the multi-vitamins are 5 to 10kg and the composite trace elements are 15 to 25kg. Long-term tests of the low-salinity cultured marine fishes in the Pearl River Delta, such as trachinotus ovatus, Japanese sea bass, sciaenops ocellatus, sparus latus, sparus macrocephalus, latescalcarfer and the like, prove that the feed additive premix for the low-salinity cultured marine fishes has comprehensive nutrition and can meet the requirement of various marine fishes on total nutrition in a low-salinity culture environment; the feed additive premix is safe and high-efficiency, does not have any toxic or side effect, makes cultured objects grow rapidly and has high feed conversion rate and good culture benefit if taken for a long time; meanwhile, the additive can obviously enhance the immunologic function and anti-stress capability of the cultured objects, and the cultured marine fishes have high vigor, bright body color and high survival rate.

The invention relates to the field of marine active substance preparation from aquatic animal processing byproduct sources, and aims to provide a simple and quick high-extraction-rate comprehensive utilization technique of marine fish bones. The technique provided by the invention comprises the following steps: processing the raw material, crushing the fish bones, extracting chondroitin sulfate, carrying out enzymolysis on the filtrate to remove proteins, inactivating enzymes at high temperature, precipitating the chondroitin sulfate, removing fat from the filter residues, extracting collagen, salting out the collagen, collecting collagen, dialyzing the collagen, and carrying out freeze-drying and the like. The invention is simple and quick, has the advantage of high extraction rate, and achieves the goal of simultaneously producing chondroitin sulfate and collagen.

The invention relates to a fish skin sauce and a method for producing the fish skin sauce. The constituents of the fish skin sauce and the percentage of each constituent are as follows: fish skin accounts for 30-33%, soy bean paste 10-13%, chili sauce 1-3%, tomato sauce 5-7%, carrot 3-5%, mushroom 1-3%, pork 3-5%, onion 10-13%, white vinegar 1-3%, cooking wine 0.5-1.5%, onion powder 0.5-1.5%, ginger powder 0.1-0.5%, oyster juice 0.5-1%, sugar 1-3%, sea fish extract 0.5-1%, skipjack powder 0.2-0.6%, salad oil 7-9%, and water in balancing amount. The fish skin sauce is made by the steps of material selecting, mixing, stir-frying, filling and packing, and sterilizing. The invention has the advantage that a more rational nutrition balance is achieved by integrating the nutrients of the raw materials such as parrot, mushroom, pork, onion and the like with the nutrients of the fish skin.

The invention discloses an alga control method for a freshwater environment based on cultivation of barracudas and mullets, relating to a water treatment method. Aiming at solving the problem that algae can not be stably and thoroughly controlled in a eutrophic fresh water body, the method comprises the following steps: at least introducing one species of estuarine / marine fishes, such as barracudas and mullets which can only be propagated in sea water, and carrying out desalination processing before cultivation to enable the barracudas and the mullets to be adapted to a freshwater living environment, wherein the mesh size in the large water body ensures whether the cultivated fishes can escape; dividing or mixing according to different ages, carrying out culture in enclosure in girds by meshes, and simultaneously cultivating and propagating silver carps, bighead carps, other local fishes and local shellfish; and propagating local common aquatic plants. Thus, the fishes can be fed on the algae in a filter-feeding mode and simultaneously swallow detritus substances, thereby achieving the purpose of thoroughly purifying the water environment. By harvesting the cultivated and propagated aquatic organisms to remove nitrogen / phosphorus nutrients in water and humus in bed material from the water body, the method disclosed by the invention greatly improves the water quality of the cultivation water body.

The invention discloses a production method of fish protein hydrolyzed chelate. The production method includes: preprocessing residual waste of low-value marine fishes such as ribbonfish, adding complex enzymes to hydrolyze the waste, and subjecting polypeptides to chelate modification. The fish protein hydrolyzed chelate has effects of antibiosis, antioxidation and ferric strengthening. The fish protein hydrolyzed chelate is purely natural, has good antioxidation, and has evident antibacterial effects on escherichia coli, bacillus subtilis, staphylococcus aureus and salmonella. Absolute ethyl alcohol can be recycled in production. Production cost is low. High-value development and high utilization of aquatic products are achieved.

The invention provides a method for preparing fish meal protein peptide. The method comprises the following steps: (1) taking defatted marine fish meal as a raw material, and adding water for dissolving according to the weight-bulk ratio of material to liquid being (1: 5)-(12.5); (2) under the temperature of 115-130 DEG C and pressure of 0.75-1.75 kg / cm<2>, cooking for 20-30 minutes; cooling to obtain cooking liquid; (3) adjusting the pH value of the cooking liquid to 7.0-9.5; adding protease, controlling the mass concentration ratio of protease to substrate to be 0.5-2.5 percent, and carrying out enzymolysis under the temperature of 45-70 DEG C to obtain enzymatic hydrolysate; and (4) heating the enzymatic hydrolysate to the temperature of 90-95 DEG C, inactivating and drying. The fish meal protein peptide is high in small-molecule oligopeptide content and digestibility. The fish meal protein peptide as a feed additive is matched with feed to be eaten by animals, so that the bioavailability of fish meal protein can be improved, the animal immunity also can be improved, and the disease resistance is enhanced.

The invention provides a uric acid reducing food composition which comprises the following components in parts by weight: 22-28 parts of marine fish oligopeptide powder, 25-35 parts of lophatherum gracile powder, 18-22 parts of poria cocos powder and 450-550 parts of soybean peptide. The composition provided by the invention has the following technical effects: 1) uric acid is reduced, and gout is relieved; 2) the composition is safe without side effects and causes no burden to body; and 3) the component marine fish oligopeptide can inhibit the synthesis of uric acid, the diuresis effect of lophatherum gracile and poria cocos can promote excretion of uric acid, and the soybean peptide supplements the proteins necessary to body and improves the basic metabolic capability and has a function of accelerating metabolism of uric acid in body.

The invention belongs to the field of research of marine fish mitochondrial genome control areas, and relates to an amplification primer of the marine fish mitochondrial genome control area. The amplification primer is composed of two chains of single-chain oligonucleotide, wherein a light-chain primer is a nucleotide sequence shown in SEQ ID No.1 and a heavy-chain primer is a nucleotide sequenceshown in SEQ ID No.2. The invention also provides a design method of the amplification primer and a method for amplifying the marine fish mitochondrial genome control area utilizing the amplificationprimer. The amplification primer provided by the invention can efficiently and specifically amplify the marine fish mitochondrial genome control area, and can be applicable to large-scale investigation and analysis of fish seed resources, thereby providing a powerful tool for rapidly and efficiently obtaining the marine fish mitochondrial genome control area sequence to carry out variety identification, seed resource investigation and control area evolution research.

The invention relates to a selective breeding method for disease-resistant superior strains of paralichthys olivaceus, which comprises group selective breeding and family selective breeding of the paralichthys olivaceus and is characterized by also comprising molecular marker-assisted selective breeding, manual gynogenesis selective breeding or inbreeding selective breeding of a disease-resistant paralichthys olivaceus family. Multiple kinds of biotechnology such as group selective breeding, intraspecific hybridization, family selective breeding, molecular marker-assisted selective breeding, manual gynogenesis selective breeding, inbreeding and the like is comprehensively adopted; a selective breeding method for the disease-resistant superior strains of marine fishes in China is established for the first time by taking the paralichthys olivaceus as a material; the superior strains of the paralichthys olivaceus of which the vibrio anguillarum resistance is obviously improved are bred; and the breeding survival rate is improved by 20 to 47 percent and the growth rate is improved by over 20 percent. The method opens up a new technical approach for the selective breeding of the disease-resistant strains of the fishes, is suitable for popularization and application to all cultured fishes, has great significance and application value for breeding the disease-resistant superior strains of the cultured fishes, and can generate great economic and social benefits.

The invention relates to seafood sauce and a preparation process thereof; fresh sea fish or sea shrimp or sea crab or sea intestine or seashell meat is taken as the base material; the base material receives enzymolysis by proteolytic enzyme and is changed into liquid material; trimeric sodium phosphate, ethyl maltol and glucose are added into the liquid material to be fermented at high temperature; maltodextrin and corn starch are taken as thickening agent, flavouring agent, preservative, stabilizing agent and water are added in, and the product is regulated and prepared into the seafood source at high temperature, the weight percent of the base material is 25 percent to 45 percent, the proteolytic enzyme is 0.05 percent to 0.1 percent, the trimeric sodium phosphate is 0.1 percent to 0.3 percent, the ethyl maltol is 0.01 percent to 0.03 percent, the glucose is 0.5 percent to 1.5 percent, the maltodextrin is 6 percent to 12 percent, the corn starch is 2 percent to 6 percent, the flavouring agent is 10 percent to 23 percent, the preservative is 0.1 percent to 0.2 percent, the stabilizing agent is 0.7 percent to 1.5 percent, and the rest is water. The method prepares the seafood sauce through enzymolysis, fermentation and regulation, and has short production cycle; the whole process is completed in a closed reaction kettle, is not affected by the season and the climate and does not produce pollution; the prepared seafood sauce is pure in color, fresh and heavy in flavor and rich in nutrition, and can meet the demand of different consumers.

The invention discloses an intelligence developmental brain tonic hygienic soft capsule and preparing method, which comprises the following parts: calico salmon carrier extract oil (1-5)%, condensed deep-marine fish oil (50-65)%, walnut oil (15-25) %, perilla oil (5-20)%, phosphatidyl serine (1-5)% and vitamin E (0.1-0.2)%. The manufacturing method comprises the following steps: (1) preparing calico salmon carrier extract oil, (2) allocating, (3) metering filled soft capsule.

The invention relates to a glycopeptide mixture product and application thereof in preparing a medicament for improving the nutritional status and immunologic function of tumor patients receiving radiotherapy and chemotherapy and health food or food. A glycopeptide mixture is prepared by mixing marine oligopeptide and bioactive polysaccharide according to certain proportion. The marine oligopeptide is a group of oligopeptide mixtures with low molecular weights which are produced by taking fish skin, fish meat or fish bone of marine fishes as main raw materials through enzymatic hydrolysis, can be dissolved by trichloroacetic acid, and have relative molecular mass less than 1,000, and has the characteristics of easy absorption, quick absorption, low viscosity, good water solubility, and the like. The bioactive polysaccharide comprises lentinan, ganoderan, grifola polysaccharide, panaxan, lycium bararum polysaccharide and algal polysaccharide. Animal experiments and clinical observation prove that the glycopeptide mixture prepared by mixing the marine oligopeptide and the bioactive polysaccharide has the effect of improving the immunologic function of tumor-bearing mice and the nutritional status of the tumor patients receiving radiotherapy and chemotherapy.

The invention discloses a light emitting diode (LED) illumination system with an adjustable color temperature for industrial fish farming. in construe, the system comprises a power supply module, a white light LED module and a color temperature adjustment module, wherein the power supply module is respectively connected with the white light LED module and the color temperature adjustment module; the color temperature adjustment module is composed of a red light LED module, a green light LED module, a blue light LED module and a current control module; and the red light LED module, the green light LED module and the blue light LED module are respectively connected with the current control module in series. The light emitting diode illumination system with adjustable color temperature for industrial fish farming has the beneficial effects that the color temperature can be adjusted according to the practical situation in the farming process so as to satisfy requirements of different marine fishes in different growth periods on light color, light intensity and the light period, thereby accelerating fishes to healthily grow and improving the economic benefit of industrial fish farming.

The invention discloses substitutional tea with the uric acid reducing effect and a preparation method thereof. The substitutional tea is prepared from the following raw materials in parts by weight:10-60 parts of chicory, 40-110 parts of corn stigma, 20-100 parts of radix puerariae, 1-12 parts of rhizoma polygonati, 1-15 parts of poria cocos, 0.1-2 parts of celery seeds, 1-10 parts of emblic leafflower fruits, 300-500 parts of semen coicis, 0.5-3 parts of marine fish oligopeptide, 0.5-3 parts of seaweed extract, 0.5-3 parts of cherry powder, 30-80 parts of fructus momordicae grosvenori, 20-70 parts of fructus crataegi, 10-35 parts of horseradish tree leaves, 20-60 parts of cranberries and 20-80 parts of honeysuckle flower, wherein part of the raw materials are extracted and then granulated and are mixed and packaged with other raw materials so as to form the substitutional tea. The substitute tea with the uric acid reducing effect has the advantages that the raw materials such as leaves, rhizomes, flowers, fruits, seeds and functional factors of a food source are scientifically proportioned, aiming at different forming reasons of uric acid, adjuvant materials are not added, in addition, the use is convenient, the taste is good, the substitutional tea has no toxic and side effects after being drunk for a long time, is suitable for being drunk by people suffering from hyperuricemia for a long time and has very good uric acid reducing effect.

The invention discloses a fish protein organic compound fertilizer and a preparation method thereof. The fish protein organic compound fertilizer is prepared by using a deep marine fish as a main raw material and adopting the steps of fermenting through microorganisms to form a liquid base fertilizer and adding copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, molybdenum salt, fulvic potassium, EDTA (Ethylene Diamine Tetraacetic Acid) and the like. The fish protein organic compound fertilizer is rich in fish protein and multiple trace elements, is favorable for improving the soil, promoting the aggregation, improving the water retention and ventilation of the soil and eliminating the hardening, and is suitable for various vegetables, fruit trees and field crops.

The invention relates to the field of preparation of marine active substance from byproducts processed by aquatic animals and aims at providing a process for extracting collagen and dermatan sulfate by comprehensively utilizing marine fish skins. The process comprises the steps of fish skin treatment, removal of impure protein of the fish skin, removal of fat of the fish skin, fish skin enzymolysis, collagen salting, collagen collection, collagen dialysis and lyophilization, dermatan sulfate glucosidic bond protection, dermatan sulfate precipitation, dermatan sulfate purification and the like. According to the process disclosed by the invention, the active substances can be extracted from the marine fish skins simply, quickly and efficiently, the aim of cogenerating the collagen with the dermatan sulfate is achieved, the utilization benefits of the fish skins are increased and economic benefits are increased. The process is simple and feasible, high in extraction efficiency and suitable for industrial production.

The invention discloses a polypeptide nutritional health-care food containing marine fish oligopeptides. The food comprises the following raw materials in percentage by weight: 20 to 35 percent of marine fish oligopeptide powder, 10 to 25 percent of soy peptide powder, 10 to 25 percent of spirulina, 10 to 15 percent of soy isolate protein, 10 to 15 percent of soyabean lecithin, 5 to 10 percent ofblack fungus powder, 5 to 10 percent of fructo-oligosaccharide, 2 to 5 percent of microcrystalline cellulose, and 1 to 3 percent of magnesium stearate. The product has wide application range, is suitable for patients with high blood sugar or diabetes mellitus, can be easily absorbed by a human body, has the effects of preventing diseases and beautifying besides high nutritional value, is advantageous to removing waste generated in the body in time and enriching the variety of polypeptide foods home and abroad.

The invention relates to a large-scale preparation method of fish scale type I collagen peptides. The preparation method comprises the following steps: firstly, performing alkali and acid pretreatment on marine fish scales or freshwater fish scales serving as raw materials to remove impurities, and directly performing directed enzymatic hydrolysis on collagen type I in the fish scales by adopting a one-step biological enzymatic hydrolysis technology to form collagen peptides type I with relatively centralized molecular weight distribution; secondly, separating, purifying and concentrating directed enzyme liquid at the normal temperature by adopting a continuous centrifugal separation and membrane separation combined technology to obtain a fish scale type I collagen peptide solution with purity of not less than 95% and molecular weight of less than 1,000 Dalton; finally, quickly drying finished fish scale type I collagen peptides by adopting a spray-drying technology. The method is simple and feasible in overall process, efficient, energy-saving, short in production cycle and suitable for large-scale production; a fish scale type I collagen peptide product produced by adopting the method is high in purity and good in quality, and the content of peptides with molecular weight of less than 1,000 Dalton is more than 97%.

The invention belongs to the field of marine fish mitochondrial genome research, and particularly relates to a marine fish mitochondrion 12S rRNA (ribosomal ribonucleic acid) gene amplification primer. The primer is composed of two single-chain oligonucleotide chains, wherein a light chain primer is a nucleotide sequence shown as SEQ ID NO.1, and a heavy chain primer is a nucleotide sequence shown as SEQ ID NO.2. The invention also provides a design method of the amplification primer and a method for amplifying a marine fish DNA (deoxyribonucleic acid) solution by using the amplification primer. The invention can efficiently and specifically amplify multiple marine fish mitochondrion 12S rRNA genes and can be used for the analytical study on the system evolution of different taxonomic categories of fishes, thereby providing a powerful tool for fish species identification, germplasm resource survey and system evolution research.