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Comprehensive utilization technique of marine fish bones

A technology of fish bones and fish, which is applied in the field of marine active substance preparation, can solve the problems of low extraction efficiency of chondroitin sulfate, complex process, and incomplete utilization of marine fish bones, etc., so as to improve utilization efficiency, increase economic benefits, The effect of efficient extraction

Active Publication Date: 2012-09-26
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses marine fish processing by-product fish bones (squid bones) to extract chondroitin sulfate alone, but does not comprehensively utilize marine fish bones, and the extraction efficiency of chondroitin sulfate is not high, and the process is relatively complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Raw material processing: remove the residual meat from the fresh anglerfish bone, and wash it in hot water at 40°C;

[0036] (2) Fishbone crushing: add NaOH solution accounting for 15% of the weight of the fishbone to the fishbone obtained in step (1), the concentration of the NaOH solution is 0.5mol / L, and add NaCl solution of 200% of the weight of the fishbone at the same time, The mass concentration of NaCl solution is 15%; Fishbone is pulverized with pulverizer, forms pulverizing liquid;

[0037] (3) Chondroitin Sulfate Extraction: Stir and extract the pulverized liquid obtained in step (2) at 50°C for 8 h, filter through a 150-mesh filter cloth, extract the obtained filter residue once more under the same conditions and filter, and combine twice The filtered filtrate is obtained as an extraction filtrate, and the filter residue obtained by the second filtration is collected for further extraction of collagen;

[0038] (4) Deproteinize the filtrate enzyme: adjust...

Embodiment 2

[0047] (1) Raw material processing: remove the residual meat from the fresh ray fish bones, and wash them in hot water at 50°C;

[0048] (2) Fishbone crushing: Add NaOH solution accounting for 10% of the weight of the fishbone to the fishbone obtained in step (1). The mass concentration of the NaCl solution is 10%; the fish bones are pulverized with a pulverizer to form pulverized liquid;

[0049] (3) Extraction of chondroitin sulfate: Stir and extract the pulverized liquid obtained in step (2) at 58°C for 6 hours, filter with a 200-mesh filter cloth, extract the obtained filter residue once more under the same conditions and filter, combine and filter twice The filtrate was obtained as the leaching filtrate, and the filter residue obtained by the second filtration was collected for further extraction of collagen;

[0050] (4) Deproteinize the filtrate enzyme: adjust the pH of the extraction filtrate obtained in step (3) to 7.5 with 4 mol / L hydrochloric acid, add neutral prot...

Embodiment 3

[0059] (1) Raw material processing: remove the residual meat from the fresh Chinese stingray bones, and wash them in hot water at 45°C;

[0060] (2) Fishbone crushing: add NaOH solution accounting for 12% of the weight of fishbone to the fishbone obtained in step (1), the concentration of NaOH solution is 0.8 mol / L, and add NaCl solution of 250% by weight of fishbone The mass concentration of the NaCl solution is 12%; the fish bones are pulverized with a pulverizer to form pulverized liquid;

[0061] (3) Chondroitin Sulfate Extraction: Stir and extract the pulverized liquid obtained in step (2) at 55°C for 7 h, filter through a 180-mesh filter cloth, extract the obtained filter residue once more under the same conditions and filter, and combine twice The filtered filtrate is obtained as an extraction filtrate, and the filter residue obtained by the second filtration is collected for further extraction of collagen;

[0062] (4) Deproteinize the filtrate enzyme: adjust the pH o...

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Abstract

The invention relates to the field of marine active substance preparation from aquatic animal processing byproduct sources, and aims to provide a simple and quick high-extraction-rate comprehensive utilization technique of marine fish bones. The technique provided by the invention comprises the following steps: processing the raw material, crushing the fish bones, extracting chondroitin sulfate, carrying out enzymolysis on the filtrate to remove proteins, inactivating enzymes at high temperature, precipitating the chondroitin sulfate, removing fat from the filter residues, extracting collagen, salting out the collagen, collecting collagen, dialyzing the collagen, and carrying out freeze-drying and the like. The invention is simple and quick, has the advantage of high extraction rate, and achieves the goal of simultaneously producing chondroitin sulfate and collagen.

Description

technical field [0001] The invention relates to the field of preparation of marine active substances derived from aquatic animal processing by-products, in particular to a comprehensive utilization process of marine fish bones. Background technique [0002] Chondroitin Sulfate (CS) is a kind of glycosaminoglycan, which is polymerized by D-glucuronic acid and N-acetyl-D-galactosamine with 1,3 glycosidic bonded disaccharide as the basic unit macromolecular polysaccharides. CS resources are abundant and widely exist in human and animal bones, cartilage, tendons, ligaments and other tissues. As a drug, CS is mainly used to treat arthritis and coronary heart disease, and also has certain effects on neuralgia, migraine, and atherosclerosis. Traditionally, chondroitin sulfate is mainly extracted from animal cartilage tissues such as pigs, cattle, and sheep, and the source and process are relatively simple. [0003] Collagen is the most abundant protein in animal sources, account...

Claims

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Application Information

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IPC IPC(8): C08B37/08C12P21/06C07K1/14C07K14/78
Inventor 付万冬廖妙飞钟明杰郑斌周宇芳
Owner ZHEJIANG MARINE DEV RES INST
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