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Method for testing toxicity of environmental estrogen on whitebait embryonic development

A technology for environmental estrogen and embryonic development, which is applied in the field of environmental estrogen toxicity testing for the development of whitebait embryos

Inactive Publication Date: 2011-03-02
SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] At present, domestic research on silverfish is still limited to its biological characteristics, proliferation and transplantation techniques, and FET testing research has not yet been carried out using silverfish embryos

Method used

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  • Method for testing toxicity of environmental estrogen on whitebait embryonic development
  • Method for testing toxicity of environmental estrogen on whitebait embryonic development
  • Method for testing toxicity of environmental estrogen on whitebait embryonic development

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The technical features of the present invention will be described in detail below in conjunction with specific embodiments and accompanying drawings.

[0036] The tested organisms are the embryos of big whitebait. The fertilized eggs of big whitebait are collected during the spawning period (from mid-December to mid-to-late March of the following year). Before the start of the experiment, the fertilized eggs were carefully moved into a glass petri dish filled with standard dilution water to spread evenly at the bottom of the petri dish, and then stored in a refrigerator at 2°C in the dark. The entire solution was changed every two days.

[0037] Pre-test

[0038] In the co-solvent methanol control group, all silverfish embryos died after 2 days of exposure, so no co-solvent was used in the formal experiment, and the maximum exposure concentration of estrogen was set at 1 mg / L (lower than the maximum solubility of estrogen in water). The development and hatching of ...

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Abstract

The invention discloses a method for testing toxicity of environmental estrogen on whitebait embryonic development. The method of the invention utilizes the sensitivity of embryo to the simulation of environmental pollutant or chemical matter to perform a test under semi-static state on whitebait embryo which is exposed in standard diluted aqueous solution of subject having a series of concentrations. The test lasts about 30 days, begins as exposing a live embryo at the blastula stage in the aqueous solution of subject, and ends with hatching all embryos in a control group and an exposed group. The toxicity endpoint contains death, monstrosity and delayed hatching. The method comprises the steps of confirming the toxicity of the subject on the whitebait embryonic development by observing the toxicity endpoint of the exposed group and comparing with the control group; researching on the influence of two natural estrogens E2 and EE2 on the whitebait embryo-yolk sac stage so as to confirm the toxic dose-effect relationship of the natural estrogens to the whitebait embryonic development, and evaluating the potential risk of this type of fish exposed under the estrogen with environmental concentration.

Description

technical field [0001] The invention relates to a toxicity testing method, in particular to a toxicity testing method of environmental estrogens on the embryonic development of whitebait, belonging to the field of fish acute toxicity testing. Background technique [0002] In the past 30 years, exogenous compounds in the environment—Environmental estrogens (Environmental estrogens) can cause health damage to human and wild animal development and reproduction, which has attracted great attention. The reported environmental estrogen hazards include : Reduced male semen, disordered sexual development of wild fish (including hermaphrodites, feminization of male fish, etc.), reduced populations of amphibians (such as alligators, toads), etc. Environmental estrogens are an important class of endocrine disruptors, which either belong to natural estrogens (that is, excreted into the environment by endogenous estrogens in organisms), or belong to artificially synthesized estrogens and...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 胡双庆沈根祥史江红孟耀斌张洪渠朱江王晓丹赵庆节郭春霞钱晓雍朱英顾海蓉王振旗
Owner SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
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