63results about How to "Improve nitrogen utilization" patented technology

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

The invention discloses an efficient multi-element compound fertilizer, which consists of hexanoic acid 2-(diethylamino) ethyl ester, polypeptide chelated potassium, polypeptide L-aspartate potassium, urea, potassium chloride, diammonium phosphate, calcium superphosphate, potassium dihydrogen phosphate, calcium-magnesium phosphate fertilizer, ammonium chloride, thiamine, zinc chloride, borax, ferrous sulphate, copper sulphate, manganese sulfate, sodium molybdate, cobalt chloride and silicon-manganese phosphate fertilizer. The efficient multi-element compound fertilizer has the advantages of promoting the growth of crops, improving the disease resistance of the crops, resisting falling, insect pests and frozen injury, improving the fertilizer effect, improving the soil, accelerating the growth of branches and leaves, reinforcing the photosynthesis, accelerating the growth of roots, enhancing the drought resistant capacity, improving the yield of the crops and improving the quality of fruits. The yield of grain crops is averagely increased by more than 26 percent, the yield of economic crops is averagely increased by more than 33 percent, and the yield of vegetable crops is averagely increased by more than 42 percent.

The invention provides a method for preparing a protein powder by utilizing proteinase to hydrolyze albumen. The method is as follows: the albumen of fresh poultry eggs is taken as raw material and stirred to obtain a protein liquid; the liquid is added with ferment for fermentation and desugarization, added with water to be diluted, and heated to be denatured; hydrolysis through alkaline protease, neutral protease and flavor enzyme is carried out and then the mixture is heated to passivate protease and dried, thus obtaining the protein powder. The method has the advantages of simple technique, convenient operation, high efficiency, low cost, high nitrogen utilization ratio, needing no debitterizing, decolorizing or desalination treatment and having significant application prospect.

The invention discloses a nitrogen fertilizer stabilizer composition. The nitrogen fertilizer stabilizer composition comprises the following components in part by weight: 1-60 parts of nitrapyrin, 1-15 parts of wall material, 2-10 parts of emulsifier, 0.05-0.3 parts of viscosity regulator, 2-10 parts of dispersing agent and 0.1-100 parts of water. The invention further provides a preparation method of the nitrogen fertilizer stabilizer composition. The preparation method of the nitrogen fertilizer stabilizer composition comprises the following steps of: heating and melting active components, adding the macromolecular wall material, and stirring uniformly; adding a mother liquid containing the active components and the wall material into an emulsifier-containing water solution under a shearing condition, and emulsifying into an oil-in-water state; and controlling grain size as required, wherein the nitrogen fertilizer stabilizer composition can be in a liquid or solid state; and liquid drops containing the active components react with water molecules to form the macromolecular wall material. Through addition of a nitrapyrin product, the nitrogen fertilizer stabilizer composition can improve the using efficiency of an ordinary nitrogen fertilizer by decreasing nitrogen leaching and denitrification, is good in compatibility with a variety of fertilizers, pesticides, weedicides and bactericides, and can be mixed therewith for application.

The present invention relates to non-naturally occurring plants that display enhanced agronomic characteristics due to elevated levels of nitrogen utilization proteins in the root of the plants. In particular, the present invention relates to non-naturally occurring plants with elevated levels of nitrogen utilization proteins specifically localized to the root epidermis of the plant. In addition, the present invention includes a preferred method of generating such non-naturally occurring plants by introducing into the plant a transgene encoding a nitrogen utilization protein operably linked to a root-epidermis-specific promoter.

The present invention relates to a nitrogen-regulated GATA transcription factor gene required for sugar sensing and the modulation of the expression of this gene to modulate a characteristic in a plant. The GATA transcription factor of the present invention is involved in regulating sugar sensing in plants and its expression is influenced by nitrogen status. Increased expression of this or substantially similar genes can produce plants with improved nitrogen utilization and increased yield.

The invention relates to composite prebiotics capable of regulating the intestinal tract function of a human body. The composite prebiotics comprise the following components in percentage by weight: 25%-35% of oligomerization isomaltose, 25%-35% of polydextrose, 10%-20% of peanut peptides, 5%-15% of fructo-oligosaccharide, 5%-15% of inulin, and 3%-7% of oligomerization xylose. According to the composite prebiotics disclosed by the invention, the fructo-oligosaccharide, the polydextrose, the oligomerization xylose, the oligomerization isomaltose, the inulin and the peanut peptide powder which are selected are in concerted application, the mixed ingredients are reasonable, the dosage is scientific, and all the components are acted jointly for regulating the intestinal tract of the human body, promoting the growth of the probiotics, providing egg white nutrition and promoting the absorption of the nutrition.

The invention provides a method for preparing cellulase by bacilli. The method includes the steps of using bacillus as a producing strain, subjecting the producing strain to slant culture, shaking culture and fermenting culture to obtain the cellulase. Fermenting culture medium comprises, by weight percent, 0.5-2% of starch, 1-5% of soybean cake meal, 8-12% of soybean cake meal hydrolysate, 0.3-0.8% of bran, 1-2% of inorganic salt, and 0-0.3% of foaming agent. The cellulase prepared by the method has high activity in neutral pH and alkali conditions and is better than acidic cellulase in terms of water abrasion enzyme application. Starch material is used as a carbon source by the method, and accordingly the cellulase is better than that of the fiber material in terms of transfer, feeding, sterilization and the like. The soybean cake meal hydrolysate is used as a soluble nitrogen source, and accordingly the cellulase is low in cost and convenient to prepare and use. The cellulase prepared by the method is low in cost, high in activity, wide in pH application range, high in stability and short in production cycle.

The present invention relates to the technical field of materials, and provides a heat-conduction filling material for the heat dissipation of electronic components, wherein the heat-conduction filling material comprises, by mass, 14-20% of a silicon rubber, 20-40% of a graphene / carbon nano-tube composite material, 30-60% of a metal oxide, 0.1-1% of a coupling agent, and 1-2% of a liquid state metal. According to the present invention, the graphene / carbon nano-tube composite material is selected as the raw material and has the three-dimensional network structure, and through the synergistic effect between the graphene and the carbon nano-tubes, the excellent performances are provided compared to the single use of any one material, wherein the excellent performances comprise good isotropic thermal conductivity, good isotropic electric conductivity, good three-dimensional space micro-pore network and the like; and the limitation problem of the thermal conduction direction due to the single use of the two materials such as graphene and carbon nano-tubes in the prior art is solved.

The invention discloses a formula of preparation method of composite peptide powder. The composite peptide powder comprises the following raw materials of hydrolyzed whey protein peptide, soybean peptide powder, wheat oligopeptides, crystalline fructose, citric acid, lemon essence, potassium citrate and sucralose. The citric acid mode of the composite peptide powder is similar to an FAO / WHO mode,and the composite peptide powder is good in absorbing effect and is suitable for crowds with disorder of digestion and absorption and needing supplementing protein.

The present invention relates to an 8% pig premix under the net energy system. A pig feed is characterized by preparing from the following raw materials in weight percentage in a recommended formula: 67% of corn, 18% of soybean meal, 7% of wheat bran, and 8% of the premix, and is prepared into a batch mixture with CP 16%, DLys 1.0% and NE2230 Kcal / kg. The premix can be applied in growing pigs of 20-50 kg, reduce NE and CP levels in daily ration and feed costs, control energy deposition and carcass fat, fully enhance the absorption and utilization rate of raw materials, significantly reduce nitrogen excretion amount, thus improve animal performance, at the same time improve pig hair color, and increase economic efficiency pig farms.

The invention discloses a method for nitriding molten steel stably, which comprises the following step of refining the molten steel in a ladle furnace (LF). The method is characterized by comprising the following step of: nitriding in an electric arc area by using a nitriding gun in the slagging process after the molten steel enters the LF, wherein the nitriding gun comprises an air blow main pipe (5), a protective pipe (7) and a gun body fixing device (6); the protective pipe (7) is approximately in a Y shape; the air blow main pipe (5) is fixed in the protective pipe (7); and a plurality ofspray orifices (13) are formed at inner arcs of arc sections (15 and 16) of the air blow main pipe (5) and arc sections (11 and 14) of the protective pipe (7). In the method, the nitriding effect is stable, and the utilization rate of nitrogen is up to over 75 percent; and the nitrogen blowing time is short, nitriding of the molten steel can be completed in the normal process of electric furnace heating without the prolonging of the smelting time, and the average nitriding amount of the molten steel is 0.0143 percent.

The present invention relates to a nitrogen-regulated GATA transcription factor gene required for sugar sensing and the modulation of the expression of this gene to modulate a characteristic in a plant. The GATA transcription factor of the present invention is involved in regulating sugar sensing in plants and its expression is influenced by nitrogen status. Increased expression of this or substantially similar genes can produce plants with improved nitrogen utilization and increased yield.

The present invention relates to a nitrogen-regulated RING-like ubiquitin E3 ligase gene required for sugar sensing and the modulation of the expression of this gene to modulate a characteristic in a plant. The RING-like ubiquitin E3 ligase of the present invention is involved in mediating nitrogen limitation adaptive responses in plants and its expression is influenced by nitrogen status. Increased expression of this or substantially similar genes can produce plants with improved nitrogen utilization and increased yield.

The invention belongs to the field of seedling culturing matrixes and relates to a functional seedling culturing matrix, in particular to a functional seedling culturing matrix and an application thereof. The functional seedling culturing matrix comprises wormcast, activated carbon, a long-acting organic fertilizer, solid water, a photosynthetic biological fertilizer and grass carbon. The designed seedling culturing matrix contains the solid water and the photosynthetic biological fertilizer; the solid water can effectively relieve the water-deficient phenomenon of the seedling culturing matrix due to the fact that the matrix is not watered for a long time and can supplement water for a long time; the photosynthetic biological fertilizer can greatly increase the utilization rate of nitrogen, also has a nitrogen fixation function, improves the ecological environment of crop root systems, has sterilization and pest inhibition functions and improves plant quality; the seedling culturing matrix is stored in a designed round plastic tank, and the action effect of the seedling culturing matrix can be further improved. The growth cycle of cultured plants can be shortened; the interest and capability of children can be cultivated, and the functional seedling culturing matrix can be used for ornament in the growth period and is very suitable for a sightseeing park.

The invention provides a method for preparing cellulase by bacilli. The method includes the steps of using bacillus as a producing strain, subjecting the producing strain to slant culture, shaking culture and fermenting culture to obtain the cellulase. Fermenting culture medium comprises, by weight percent, 0.5-2% of starch, 1-5% of soybean cake meal, 8-12% of soybean cake meal hydrolysate, 0.3-0.8% of bran, 1-2% of inorganic salt, and 0-0.3% of foaming agent. The cellulase prepared by the method has high activity in neutral pH and alkali conditions and is better than acidic cellulase in terms of water abrasion enzyme application. Starch material is used as a carbon source by the method, and accordingly the cellulase is better than that of the fiber material in terms of transfer, feeding, sterilization and the like. The soybean cake meal hydrolysate is used as a soluble nitrogen source, and accordingly the cellulase is low in cost and convenient to prepare and use. The cellulase prepared by the method is low in cost, high in activity, wide in pH application range, high in stability and short in production cycle.

Plant growth enhancing mixture and method of selectively timing the application of same during the development of crop plants or other plants to positively augment cell number increase and cellular development of crop plants or other plants to enhance development and / or productivity of the economic portion of the crop plant or other plant. Application of the plant growth enhancing mixture at flowering enhances both weak flowers and normally strong flowers. The plant growth enhancing mixture and method of application have also been shown to impart varying disease resistance to the treated crop or other plants. The plant growth enhancing mixture and method of application also increases the depth and strength of rooting for greater access and transport of water and nutrients for growth and productivity of the crop plant.

The invention provides a high-temperature-resistant nitrification inhibitor as well as a preparation method and application thereof. The nitrification inhibitor is prepared from the following raw materials in parts by weight through reaction: 0.5-1.5 parts of a compound initiator, 1-10 parts of silicate, 3-15 parts of inorganic acid, 2-10 parts of a pyrazole derivative, 5-15 parts of organic acidcontaining carbon-carbon double bonds and 20-50 parts of solvent water. The preparation method is simple, efficient, free of pollution and low in cost, extra inert protective gas does not need to be injected, potential risks in the industrial production process are reduced, and the cost is further reduced. The nitrification inhibitor is good in stability, less in residue in agricultural products,small in toxicity, low in environmental pollution and capable of effectively improving the nitrogen utilization rate, and has the effects of dissolving phosphorus, preventing phosphorus from being fixed by soil and the like. The nitrification inhibitor also has the characteristic of high temperature resistance and can participate in the preparation process of a high-tower melt granulation compoundfertilizer, so that the comprehensive performance of the high-tower melt granulation compound fertilizer is improved.

The invention particularly relates to a nitrogen-filling electroslag process for a cavity of a nitrogen-containing steel hollow blank, which is different from a common process in which nitrogen is added into an electrode blank of nitrogen-containing steel and then electroslag is carried out. The process is characterized in that nitrogen is not added during smelting of an electrode blank, a nitrogen-free hollow electrode blank is adopted, nitrogen is injected from a reserved hole during electroslag, nitrogen is added into steel through the physical and chemical reaction of nitrogen and steel slag, the nitrogen flow is controlled according to the nitrogen content of different steel types, and alloying of the nitrogen element is completed in the electroslag remelting process. The process can be applied to common electroslag equipment only, and a special pressurizing electroslag furnace high in price does not need to be arranged. Due to the fact that the blank is a hollow blank, the hot spot is not in the center of a molten pool, the shape of an electroslag molten pool is greatly improved, the molten pool tends to be in a flat state, the consistency of internal and external crystalline states of an ingot is higher, redundant nitrogen overflows from the periphery to form a protective atmosphere, oxygen increasing and hydrogen increasing of molten steel are avoided, the alloying target of the nitrogen element can be easily achieved, and the quality of the electroslag ingot can be improved.

The invention relates to the technical field of fertilizer, in particular to an efficient environment-friendly nitrogen fertilizer based on biomass charcoal and a production process thereof. The efficient environment-friendly nitrogen fertilizer is prepared from, by weight, 100 parts of urea, 1-5 parts of biomass charcoal, 0.1-0.5 part of phosphoric acid, 1-5 parts of dicyandiamide and 0.5-1.5 parts of starch gel. The efficient environment-friendly nitrogen fertilizer has a slow release function and is long-acting, ammonia gas volatilization speed is low, leaching loss of nitrate is small, the nitrogen fertilizer can be efficiently absorbed by plants, and the nitrogen use ratio is large.

The invention discloses a biological organic fertilizer as well as a production method and application thereof, belonging to the field of fertilizer. The biological organic fertilizer contains the following components of: not less than 28.0 percent of organic matter (counted by dry basis), not less than 5 percent of nitrogen and not more than 20.0 percent of water, wherein the effective viable count (cfu) is not less than 0.33 hundred million / gram (milliliter); the pH value is 5.5-7; the fecal coligroup count is not more than 100 / gram (milliliter); the mortality rate of ascaris eggs is not less than 90 percent; and the validity period is not less than 6 months. The biological organic fertilizer protected by the invention has effects of microbial fertilizer and organic fertilizer; and wastes can be changed into valuables so as to protect environment.

The invention relates to a method for determining gas channeling of unit nitrogen flooding in a fractured-vuggy reservoir and a method for preventing channeling in injection and production. The methodfor determining gas channeling comprises the following steps of step 1, gas channeling determination principles: (1) the nitrogen content in produced gas of a production well is greater than 10%, andthe nitrogen content when nitrogen flooding is not implemented is not greater than 5%, which are both volume fractions; and (2) the gas production of the production well is greater than 1.5 times that when nitrogen flooding is not implemented; and step 2, determination that gas channeling occurs in the unit nitrogen flooding when the unit nitrogen flooding with high gas channeling risks at leastsatisfies any gas channeling determination principle, wherein the unit nitrogen flooding with the high gas channeling risks comprises at least one easy gas channeling factor that there are cross-wellfracture zones or secondary fractures between wells, the injection-production relationship is low injection and high production, and there is no structural high point or dip angle in a local structural form between the wells. If the gas channeling of the unit nitrogen flooding is determined by the above-mentioned method for determining gas channeling, the method for preventing channeling in the mode of first dynamically adjusting channeling prevention and then automatically preventing channeling or automatically preventing channeling directly is used based on the characteristics of gas channeling and the degree of gas channeling of the unit nitrogen flooding.

The present invention relates to a method for judging gas channeling in fractured-cavity reservoir units with nitrogen drive and an anti-channeling method for injection and production. 10%, and the nitrogen content is not more than 5% when nitrogen flooding is not implemented, both are volume fractions; ②The gas production of production wells is greater than 1.5 times that of when nitrogen flooding is not implemented; Step 2, when nitrogen flooding in units with high gas channeling risks If any gas channeling judgment principle is met, it is judged that gas channeling has occurred in unit nitrogen flooding, and unit nitrogen gas flooding with high risk of gas channeling includes at least one easy gas channeling factor: there is a cross-well fault zone or secondary fault between wells, and the injection-production relationship is low There is no structural high point or a dip in the local structural form between high injection and production wells. If gas channeling in unit nitrogen drive is judged by the above gas channeling determination method, then based on the gas channeling characteristics and gas channeling degree of unit nitrogen drive, first dynamically adjust the anti channeling method, followed by active channeling prevention or direct active channeling prevention method.

The present invention provides a method for changing nitrogen utilization efficiency in a plant comprises regulating the expression of Arabidopsis NRT 1.7 or an orthologue thereof so that the nitrate remobilization from older leaves to young leaves in the plant is regulated, thereby the nitrogen utilization efficiency is changed. The present invention also provides a transgenic plant obtainable by transforming a plant with an expression construct with a high or low level of expression of NRT 1.7. On the other hand, the present invention yet provides a chimera nitrate transporter, a DNA molecule coding for this chimera transporter and an expression vector thereof.

This invention relates to methods and materials for providing a benefit to a plant by associating the plant with a complex endophyte comprising a host fungus further comprising a component bacterium, including benefits to a plant derived from a seed or other plant element treated with a complex endophyte. For example, this invention provides purified complex endophytes, purified complex endophyte components such as bacteria or fungi, synthetic combinations comprising said complex endophytes and / or components, and methods of making and using the same.

The invention relates to the technical field of biological organic bacterial fertilizers, and discloses a composite biological organic bacterial fertilizer and a preparation method thereof. The composite biological organic bacterial fertilizer comprises the following raw materials in parts by weight: 88-176 parts of crop straws, 12-22 parts of rape seed cakes, 5-14 parts of a straw fermenting agent, 200-360 parts of water, 18-26 parts of a chemical fertilizer, 7-16 parts of a mixed microbial agent, 2-7 parts of acrylic acid, 3-5 parts of potassium persulfate, 6-14 parts of polyacrylamide, 5-9 parts of tetramethrin and 2-8 parts of permethrin. The composite biological organic bacterial fertilizer of the invention realizes the mechanism of improving the nitrogen supply capability of soil by applying the straw bio-organic fertilizer and the inorganic nitrogen fertilizer, promotes the absorption of plants to nutrients, has the effects of inhibiting and killing soil pathogenic microorganisms, improving the disease resistance of crops and improving the continuous cropping harm, provides a healthy growth environment for plants, has good pest resistance, protects the growth environment of the plants, and improves the survival rate of the plants.

The invention discloses a photocatalytic foliar fertilization method, relating to agriculture technology. To be specific, the solution containing photocatalysts and polyols is sprayed on the leaves of crops to provide nitrogen fertilizer under sunlight; the photocatalysts are nanocatalysts responding to the sunlight spectrum, of which the conduction band position is lower than −0.092 V(vs NHE); the mass concentration of photocatalysts in the solution is 100˜2000 mg / L, and the volume fraction of polyols accounts for 1%˜20%. In the invention, with the introduction of hole sacrificial agents to constrain the annihilation of photogenerated carriers, the electrons can be generated over the catalysts under sunlight and then react with dinitrogen to form ammonia as nitrogen fertilizer on the leaves of crops. This method has no demand for extra supplementation of nitrogenous fertilizer. Besides, it improves the utilization rate of nitrogen with a simple, secure and convenient fertilization.

The invention discloses fodder for improving nitrogen utilization rate and production performance of diary cows. The fodder is composed of basic materials and an auxiliary material, wherein the basic materials comprise the following components in parts by weight: 24.2 to 25 parts of silage corn, 13.0 to 15 parts of alfalfa, 5.4 to 6.0 parts of leymus chinensis, 2.0 to 4.0 parts of cottonseed cake, 3.6 to 4.0 parts of bagasse, 15.0 to 15.2 parts of corn, 2.0 to 2.4 parts of wheat bran, 5.07 to 5.47 parts of barley, 2.00 to 2.60 parts of soybean meal, 7.4 to 8.0 parts of bean cake, 5.0 to 5.6 parts of cottonseed meal, 3.00 to 3.08 parts of distillers' grains, 0.71 to 0.91 part of soybean curb residue, 3.0 to 4.0 parts of rapeseed meal, 0.02 to 0.06 part of methionine, 0.31 to 0.71 part of premix, 0.31 to 0.71 part of salt and 0.40 to 0.80 part of calcium bicarbonate; the auxiliary material comprises the component of 0.67 to 1.07 parts of N-carbamylglutamic acid.

The invention discloses a method for nitriding molten steel stably, which comprises the following step of refining the molten steel in a ladle furnace (LF). The method is characterized by comprising the following step of: nitriding in an electric arc area by using a nitriding gun in the slagging process after the molten steel enters the LF, wherein the nitriding gun comprises an air blow main pipe (5), a protective pipe (7) and a gun body fixing device (6); the protective pipe (7) is approximately in a Y shape; the air blow main pipe (5) is fixed in the protective pipe (7); and a plurality ofspray orifices (13) are formed at inner arcs of arc sections (15 and 16) of the air blow main pipe (5) and arc sections (11 and 14) of the protective pipe (7). In the method, the nitriding effect is stable, and the utilization rate of nitrogen is up to over 75 percent; and the nitrogen blowing time is short, nitriding of the molten steel can be completed in the normal process of electric furnace heating without the prolonging of the smelting time, and the average nitriding amount of the molten steel is 0.0143 percent.