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92results about How to "High in lysine" patented technology

Novel Insecticidal Proteins and Methods for Their Use

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
Owner:PIONEER HI BRED INT INC

Eel bone, barbary wolfberry and water spinach juice noodles and preparation method thereof

The invention discloses eel bone, barbary wolfberry and water spinach juice noodles. The noodles are prepared from raw materials in parts by weight as follows: 300-350 parts of flour, 30-33 parts of gluten powder, 9-20 parts of pollen, 13-16 parts of corn peel dietary fiber, 28-35 parts of eel bones, 5-6 parts of konjac flour, 23-25 parts of barbary wolfberry fruit powder, 20-22 parts of water spinach, 3-4 parts of condensed milk, 2-3.2 parts of sharpleaf galangal fruits, 0.4-1.3 parts of palmleaf raspberry fruits, 2-2.4 parts of elm seeds and proper amounts of table salt and papain. The noodles taste fragrant and soft, are chewy and have good elasticity, and food and medicinal materials such as oroxylum seeds, Murdannia simplex, corydalis roots and the like are also added, so that the noodles have health-care functions of nourishing yin, clearing heat, moistening the lung, soothing the liver, harmonizing the stomach and tonifying the spleen.
Owner:许菲

Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants

This invention relates to four chimeric genes, a first encoding lysine-insensitive aspartokinase (AK), which is operably linked to a plant chloroplast transit sequence, a second encoding lysine-insensitive dihydrodipicolinic acid synthase (DHDPS), which is operably linked to a plant chloroplast transit sequence, a third encoding a lysine-rich protein, and a fourth encoding a plant lysine ketoglutarate reductase, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine or threonine in the seeds of transformed plants are provided. Also provided are transformed plants wherein the seeds accumulate lysine or threonine to higher levels than untransformed plants.
Owner:EI DU PONT DE NEMOURS & CO

Grain milk and manufacturing method thereof

The invention discloses a grain milk and a manufacturing method thereof. The grain milk is prepared from the following raw materials in parts by weight: 30-50 parts of sesame, 20-40 parts of red bean, 400-600 parts of fresh milk, 200-300 parts of water, and 10-30 parts of semen coicis. The manufacturing method comprises the following steps: 1) putting 30-50 parts by weight of sesame, 20-40 parts by weight of red bean, 10-30 parts by weight of semen coicis and 300 parts by weight of water into an agitator for pulping, and filtering to obtain a mixed liquid; and 2) uniformly mixing the obtained mixed liquid and 400-600 parts by weight of milk, boiling the mixture with small fire, and waiting for cooling down to obtain the grain milk. The preparation method of the grain milk is simple, and the grain milk is high in nutritive value, does not contain any chemical additive, has rich tastes, and is a green and delicious healthy drink.
Owner:杨晨

High protein supplement

ActiveUS7982066B2Prevention and improvementImbalance conditionOrganic active ingredientsNervous disorderProtein C deficiencyProtein
The present disclosure relates to high protein dietary supplements for treating various symptoms and diseases associated with protein deficiency including weight gain, obesity, catabolic diseases, fibromyalgia, anxiety reactions, posttraumatic stress and chronic fatigue syndrome. Embodiments of dietary supplements comprise combinations of proteins, essential and semi-essential amino acids including L-Lysine, L-Arginine, and / or L-Histidine.
Owner:SCHEELE GEORGE

Transgenic plant seed with increased lysine

Disclosed herein are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase and a gene suppression molecule for lysine ketoglutarate reductase, the activity of which results in increased lysine in a plant seed.
Owner:MONSANTO TECH LLC

Planting method of selenium-rich millet

The invention discloses a planting method of selenium-rich millet. The method comprises the steps that 1, soil preparation and fertilization are carried out, wherein deep ploughing is carried out in autumn, harrowing and ridging are carried out after ploughing, and vibrating compaction is carried out in time after ridging; 2, seeds are treated, wherein the seeds are aired for 24 h before being sown, then added into a mixed solution to be soaked for 8 h at the temperature of 45-48 DEG C, and taken out to be dried in the air; 3, planting is carried out, wherein 0.65-0.7 kg of seeds are sown in each mu of land, the millet seed sowing depth is 3-4 cm, and the line spacing is 45 cm; 4, topdressing is carried out, wherein 45-50 kg of organic fertilizer is evenly spread in each mu of land in the booting stage 15 days before head sprouting; 5, harvesting is carried out, wherein harvesting is carried out after grains are completely yellow and hardened. By means of the planting method of selenium-rich millet, the millet yield can be obviously improved, lysine content, crude fat content and protein content are improved, particularly, the selenium content in millet can be remarkably improved, the amylase ratio is increased, and the nutrient and taste of millet are improved.
Owner:吴优

Rabbit excrement fermented leaf fertilizer and preparation method thereof

The invention relates to a rabbit excrement fermented leaf fertilizer and a preparation method of the rabbit excrement fermented leaf fertilizer. The rabbit excrement fermented leaf fertilizer comprises the following components in parts by weight: 5-15 parts by weight of rabbit excrement, 0.1-0.4 part by weight of zinc sulfate, 0.01-0.05 part by weight of EM (Effective Microorganisms) stock solution and 50-90 parts by weight of water. The leaf fertilizer provided by the invention has multiple fertilizer efficiencies, can increase zinc content of crops, contents of zinc content, soluble sugar and lysine, can improve quality of the crops, has an effect of removing insect, and is capable of removing plant insect attack. Because of multiple functions of the leaf fertilizer provided by the invention, labor and economic expense in cultivation can be saved, and the production cost of crops is favorably lowered. The rabbit excrement fermented leaf fertilizer is completely prepared from green raw materials without bringing health hazard caused by pesticide residue.
Owner:HEBEI AGRICULTURAL UNIV.

Method of increasing lysine content in paddy rice seed and special carrier

This invention discloses method and carrier for improving the content of lysine in rice seeds. The carrier is a plant expression carrier containing a rice seed specific promoter, dihydropicolinic acid synthetase gene and rice lysine-oxoglutaric acid reductases / saccharopin dehydrogenase gene interfering RNA coding sequence. The said dihydropicolinic acid synthetase gene is located at the downstream of the rice seed specific promoter, and the said rice lysine-oxoglutaric acid reductases / saccharopin dehydrogenase gene interfering RNA coding sequence is located at the downstream of the constitutive promoter. The genetically modified rice strains obtained in this invention have much higher free lysine content in seeds (10 times higher than that of Wild rice). Besides, the exogenous gene has no effect on rice growth. The method and the carrier have important applications in improving the lysine content in rice seeds, and provide a new way for modifying rice and other plants.
Owner:BEIJING KAITUO DNA BIOTECH RES CENT

Batata porridge and production method thereof

InactiveCN101390599AReduce "astringent" taste substances"Astringent" flavor material makes sweet potato porridge of the present invention have no astringent tasteFood preparationBiotechnologyFlatulence
The invention discloses a sweet potato porridge and a production method thereof; the raw material of the sweet potato porridge comprises raw sweet potatoes after aging treatment; the raw sweet potatoes after aging treatment are the sweet potatoes at the beginning of germination when the raw fresh sweet potatoes are placed in the embryonic environment. The production method comprises the following steps: (1) aging treatment of raw fresh potatoes, that is, the raw fresh sweet potatoes are stored at 16-20 DEG C and under a 85-90% relative humidity and turned into the raw sweet potatoes after aging treatment at the beginning of germination; (2) the raw potatoes obtained in step (1) are peeled, cut into small cubes and then cased in a jar; (3) rice is washed and put into the jar; (4) coarse cereals are washed and / or soaked and then put into the jar; (5) water is added into the jar, and a top gap is retained, following by the steps of mouth sealing, sterilizing, porridge boiling and cooling. The sweet potato porridge tastes delicious and unsour and has rich taste of sweet potato, without flatulence and hiccup after consumption.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

Jack fruit seed whole flour cookies and preparing method thereof

The invention discloses jack fruit seed whole flour cookies and a preparing method thereof. The jack fruit seed whole flour cookies are prepared from, by weight, 110-130 parts of low-gluten flour, 50-80 parts of powdered sugar, 40-60 parts of eggs, 60-100 parts of jack fruit seeds and 60-80 parts of butter. According to the technical scheme, the jack fruit seed whole flour is utilized to produce the cookies, the jack fruit seed starch is low in water retention rate, the quality of the cookies is improved, and the additive amount of the butter is effectively reduced; the nutrients of the jack fruit seed whole flour are made full use of, the jack fruit seed whole flour is high in lysine content, the lack of lysine in grains is made up, the efficiency ratio of protein can be increased, and the protein contained in the jack fruit seed whole flour does not form a gluten network; the jack fruit seed flour is high in content of resistant starch, can effectively lower the glycemic index of the cookies, and has a very good health-care function; the jack fruit seed whole flour cookies are pure in scent, not sticky and loose in mouthfeel, can be produced in large scale, and can be widely circulated, and nutritional utilization of the jack fruit seeds is promoted.
Owner:海南省农垦科学院集团有限公司 +1

Preparation method of broad bean aqueous extract feed, product and application of broad bean aqueous extract feed in embrittlement of fish muscles

The invention relates to a preparation method of broad bean aqueous extract feed, a product and application of the broad bean aqueous extract feed in embrittlement of fish muscles. The method comprises the following steps: preparation of broad bean powder, preparation of a saturated calcium hydroxide solution, preparation of 4M hydrochloric acid, extraction of a broad bean aqueous extract, preparation of broad bean aqueous extract powder, preparation of compound feed powder, preparation of a mixture of a compound feed and the broad bean aqueous extract, and preparation of a broad bean aqueousextract feed. The broad bean extract feed extracted by the method is used for fishes, can significantly improve the muscle hardness, chewiness and cohesiveness of the fishes and promote the growth performance of the fishes, and is an innovative application in fish culture.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Floury 2 gene-specific assay in maize for floury (FL2) trait introgression

ActiveUS20160153055A1Reliably and predictably introgressingHigh in lysineBryophytesMicrobiological testing/measurementMolecular breedingGermplasm
The disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more floury2 (fl2) mutations. In embodiments, the disclosure concerns a gene specific PCR-based molecular (KASPar®) assay for identifying the floury2 trait in plants. In certain embodiments, compositions and methods are disclosed for determining the zygosity of corn plants with respect to the fl2 allele. In particular embodiments, the assay may be used for fl2 germplasm identification, accelerating introgression and molecular breeding programs in corn and other plants.
Owner:CORTEVA AGRISCIENCE LLC

Compound fermented feed additive for egg-laying poultry as well as preparation method and application of additive

The invention discloses a compound fermented feed additive for egg-laying poultry as well as a preparation method and an application of the additive and belongs to the technical field of feed additives. The compound fermented feed additive for egg-laying poultry is prepared from components in parts by weight as follows: 22-25 parts of fermented sunflower seed meal, 15-20 parts of fermented alga powder, 2-4 parts of oyster powder, 1-3 parts of yellow mealworm powder, 0.05-0.1 part of soybean isoflavone, 0.1-0.3 part of carboxymethyl chitosan and 0.05-0.1 part of perilla oil. The compound fermented feed additive for egg-laying poultry can increase the egg laying rate of the poultry, improve the egg laying property and further improve the laid egg quality. The compound fermented feed additivecan promote digestive absorption of nutrient substances and enhance immunity by regulating internal environments of intestinal tracts of the poultry and promoting growth and propagation of probiotics.
Owner:QINGDAO AGRI UNIV

Method for producing oyster mushrooms with high lysine content through purple perilla straw

The invention discloses a method for producing oyster mushrooms with the high lysine content through purple perilla straw. According to the method, after the purple perilla straw is mixed with lime water and fermented, the mixture is mixed with cottonseed hulls to manufacture an edible fungi compost, the edible fungi compost is sterilized at high temperature after being bagged, an oyster mushroom strain is inoculated, and cultivation management is carried out in a conventional method. Compared with oyster mushrooms cultivated through a traditional cottonseed hull compost, the oyster mushrooms cultivated in the method is remarkably improved in the content of lysine, has the advantages of being short in growth period, good in quality, high in yield, rich in nutrition and the like, can meet the requirement of the market for high-quality oyster mushrooms special for supplementing amino acid, and provides an efficient way for comprehensive utilization of purple perilla.
Owner:山西金紫苏生物科技股份有限公司

Tilapia feed

InactiveCN102461738AHomoserine contentHigh Isoleucine ContentFood processingAnimal feeding stuffMeat and bone mealCottonseed
The invention discloses a tilapia feed. The tilapia feed comprises the following components, wherein the components comprise, by weight, 3-6 parts of hydrolyzed feather meal, 4-10 parts of soybean powder, 20-25 parts of cottonseed powder, 35-40 parts of rice, 5-10 parts of crude wheat powder, 5-10 parts of meat and bone meal, 6-10 parts of dried vinasse, 5-10 parts of peanut powder, 5-10 parts of a mineral premixing additive, and 2-6 parts of a vitamin premixing additive. According to the present invention, the formula of the tilapia feed of the present invention is scientific, the nutrition is balanced, and the tilapia can grow rapidly with the feed.
Owner:罗朝雯

Chicken feed

InactiveCN104247866AHigh white matter contentImprove qualityAnimal feeding stuffDiseaseAnimal science
The invention discloses a chicken feed. The chicken feed comprises 1.5-3.5wt% of grass meal and 0.05-0.3wt% of plant extract as effective components; the grass meal is composed of corncob powder, alfalfa meal and purslane powder; and the plant extract is a green powdery substance prepared through the steps of crushing and pulping fresh corncobs and purslane, pressing through a presser, collecting the obtained juice, and drying. The chicken feed has nutritional and medicinal functions, improves the body immunity, reduces diseases in the breeding process, reduces the use of drugs, and makes safe animal products produced. The chicken feed reduces the respiratory diseases, vent picking, feather picking and other phenomena of chickens in the breeding process, improves the conversion rate of feeds, reduces the concentration of ammonia in excreta, improves the commodity, reduces the abdominal fat percentage, improves the quality of meat, saves the feeds, reduces the dosage, reduces the feeding cost, and improves the chicken raising benefit.
Owner:尹伟

Transgenic maize encoding an endosperm specific prolamin box binding factor peptide, which produces seed with increased methionine or lysine conent

The invention provides a fertile transgenic monocot, e.g., maize, plant encoding an endosperm specific prolamin box binding factor peptide (PBF) that is expressed so as to increase the methionine and / or lysine content of the seeds of said plant over said content in the seeds of the corresponding plant that lacks said gene, wherein said corresponding plant was used to prepare said transgenic plant, or an ancestor of said transgenic plant.
Owner:RGT UNIV OF CALIFORNIA

Expression vector for improving content and quality of protein in rice corn and its making method and use

InactiveCN1966691AAvoidance of an important constraint for nutritional quality improvementImprove nutritional qualityFermentationVector-based foreign material introductionAgricultural scienceDNA fragmentation
The invention discloses an expression vector for increasing rice protein content and quality and its production method and application, having the structure and characteristics as a and b shown. The vector contains glutelin gene promoter, soybean Gy7 full length gene or Gy7 gene coding sequence cDNA fragment, terminator and incision enzyme sensitive sequence or marker gene sequence. The vector can be used for transforming Gramineae plants like rice to obtain rice variety containing high protein content and high restriction amino acid content.
Owner:SHANGHAI NORMAL UNIVERSITY

Skin-moistening and hair-blackening fresh flower noodles and preparation method of fresh flower noodles

The invention discloses skin-moistening and hair-blackening fresh flower noodles. The skin-moistening and hair-blackening fresh flower noodles are prepared from the following raw materials in parts by weight: 300-350 parts of flour, 30-33 parts of vital gluten, 9-20 parts of pollen, 13-16 parts of corn peel dietary fiber, 28-35 parts of ricefield eel bone, 17-18 parts of raspberry, 25-26 parts of rose petals, 14-16 parts of lemon juice, 5-7 parts of honey, 1.8-2.1 parts of radix rehmanniae praeparata, 2.2-2.3 parts of flower buds of panax notoginseng, 2-2.4 parts of corn poppy and a proper amount of table salt and papain. The fresh flower noodles are aromatic, soft and chewy while being eaten and have good elasticity; and by adding the edible medicinal materials including radix rehmanniae praeparata, the flower buds of panax notoginseng, the corn poppy and the like, the fresh flower noodles are endowed with health efficacies of soothing the liver, clearing heat, reducing blood pressure, relieving sore throat, nourishing yin and blood, boosting essence, filling marrow, enriching yin and nourishing kidneys.
Owner:庐江县海神面业有限公司

Method for improving content of protein and combined lysine in wheat seeds

The invention relates to the field of wheat breeding, in particular to a method for improving content of protein and combined lysine in wheat seeds, which guides high chili lysine protein gene (cflr) into conventional wheat by adopting a genetic engineering method and expresses the gene to improve the content of the protein and the combined lysine in the wheat seeds. The method is characterized by comprising the following steps of: constructing an expression vector pAHC25-Cf1r of the high chili lysine protein gene (cflr); guiding the pAHC25-Cf1r into an acceptor wheat embryo cell through a gene gun to perform callus induction and plant regeneration on the wheat embryo cell so as to obtain a T5-generation transgenic homozygous strain; and detecting cflr gene expression abundance in progeny seeds of the T5-generation transgenic homozygous strain, measuring the content of the protein and the combined lysine in the seeds, and screening the strains of which the content of the protein and the combined lysine in the seeds is remarkably improved. Compared with the conventional germ plasm breeding method, the method has the advantages of short breeding cycle, obvious improvement effect and little influence on important agronomic traits.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Honeysuckle intelligence-improving and restlessness-relieving nut flour and preparation method thereof

The invention discloses honeysuckle intelligence-improving and restlessness-relieving nut flour which is prepared from the following raw materials in parts by weight: 300-350 parts of flour, 30-33 parts of wheat gluten powder, 9-20 parts of pollen, 13-16 parts of corn peel dietary fiber, 28-35 parts of ricefield eel bone, 17-18 parts of sesame, 17-18 parts of walnut kernels, 20-22 parts of corn kernels, 15-17 parts of shrimps, 2-3 parts of elecampane, 2.2-2.3 parts of red orange, 2.3-2.4 parts of honeysuckle and appropriate table salt and papain. The nut flour disclosed by the invention is aromatic and soft and also chewy with good elasticity; and by adding the edible medicinal materials such as elecampane, red orange and honeysuckle, the nut flour has the healthcare functions of invigorating the spleen and harmonizing the stomach, regulating qi and relieving depression and relieving restlessness and resolving phlegm.
Owner:庐江县海神面业有限公司

High-protein cassava feed and preparation method thereof

InactiveCN109329568AImprove the shortcomings of poor palatabilityIncrease feed intakeAnimal feeding stuffBiotechnologyFermentation
The invention provides a high-protein cassava feed. The feed comprises the following raw materials: a cassava solid culture medium and a culture solution. The invention also provides a preparation method of the feed. According to the high-protein cassava feed disclosed by the invention, cassava protein fermented by yeast is good in taste, and can obviously improve the defect of poor palatability of cassava, and improve the feed intake of animals. According to the high-protein cassava feed disclosed by the invention, the cassava protein with high protein content can be obtained by using a single strain fermentation mode. The method can fully utilize local feed resources, save production cost, and improve animal production.
Owner:湖南赛福资源饲料科技有限公司 +1

Construction method of high-lysine protein gene SB401 expression vector and application thereof

The invention relates to construction of a high-lysine protein gene SB401 expression vector and application thereof and aims to solve the technical problems of efficient introduction of an exogenous high-lysine protein gene into a plant and efficient expression of the protein gene. The method comprises the following steps of: extracting ribonucleic acid (RNA) of a potato, obtaining full-length complementary deoxyribonucleic acid (CDNA) of the potato through reverse transcription, obtaining a sb401 gene by a polymerase chain reaction (PCR) method, recombining the gene and cloning the recombined gene into a plant dual-element expression vector; and introducing the gene into a corn gene group by an agrobacterium-mediated method, obtaining an expression transgenosis positive seedling and performing efficient expression so as to increase the lysine content of corn and further enhance the quality of corn. The method has unique advantages of low cost, small copy number, high repeatability, a small number of gene silencing phenomena, short transformation period, capability of transforming large fragments and the like.
Owner:HENAN ACAD OF AGRI SCI

High-protein composition, high-protein pet food and preparation method of high-protein pet food

The invention discloses high-protein composition, high-protein pet food and a preparation method of the high-protein pet food, and relates to the field of pet food. The high-protein composition is made of edible insect protein. Compared with traditional meat, the high-protein composition is made of the edible insect protein, has the advantages that edible insects are widely sourced and are naturalproducts, a large quantity of resources need not to be input, and emission of greenhouse gases can be reduced. Moreover, the edible insect protein has the advantages of high protein, low fat and lowcholesterol, and the high-protein pet food can completely replace traditional meat to provide pets with nutrients and energy required by the pets every day.
Owner:青岛亚鲁特食品有限公司

Method for controlling the digestive coagulation of proteins

This invention relates to the coagulation of protein containing nutritional compositions in the upper gastro-intestinal tract, more in particular in the stomach and provides a method for reducing such coagulation.
Owner:NUTRICIA

High-yield cultivation method for needle mushrooms

The invention discloses a high-yield cultivation method for needle mushrooms. The method comprises following steps: preparation of growth mediums, package, cold storage treatment, normal temperature, inoculation, bacteria growth, acceleration of mushrooms, fruiting and harvesting. Medium raw material is adopted and free from pollution and full of nutrition. During a cultivation process, no fertilizers, pesticides, disinfectors and other chemical agents is used such that damage of chemical agents to human bodies is avoided. While ensuring health of human bodies, environment pollution is avoided. Nutrition value of needle mushrooms is ensured. Fruiting rate is greatly increased and yield is improved. The growth medium prepared by the method facilitates absorption of roots for needle mushrooms and shortens growth period of needle mushrooms and increase stress resistance of needle mushrooms.
Owner:安徽金豪生态农业科技有限公司

Microbial organic fertilizer containing modified municipal sludge, and preparation method thereof

ActiveCN105061037AHigh VE contentIncrease tryptophan contentFertilizer mixturesCelluloseNutrients substances
The invention provides a microbial organic fertilizer containing modified municipal sludge, and a preparation method thereof. The microbial organic fertilizer comprises bioactive peptides, modified municipal sludge, chicken bones, cottonseed powder, wheat bran, waste molasses, vinyltrimethoxy silane, Scenedesmus acuminatus, polyamine cross-linked cellulose resin, honeysuckle flower extract, auxin, biologic humic acid, earthworm globulin and a composite bacterium agent. The effective quantity of live bacteria in the microbial organic fertilizer prepared in the invention is 0.83-0.96 billion / g, the total nitrogen content is 7-10wt%, the total phosphorus content is 3-5wt%, the total potassium content is 8-11wt%, the content of organic matters is 52.6-53.9wt%, the water content is 2.0-2.5wt%, the particle strength is 10.8-11.5N, and the percentage of contaminating microorganism is 2.1-2.6%; and the microbial organic fertilizer can effectively improve the output of agricultural products and the content of nutrients, and has an insecticidal effect.
Owner:SHANDONG WOKEQI IND CO LTD
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